Abstract：
:The possible role of cytokines in leprosy reactions was investigated by analysing the levels of tumour necrosis factor (TNF) and interleukin-1 (IL-1) in serum samples from 39 leprosy patients, 22 of them presenting either type I (upgrading) or type II (ENL) reactions. Fifty per cent of the patients showed elevated concentrations of TNF and IL-1 in at least one of the serum samples tested. This included all four patients undergoing type I reversal reaction and nine (50%) of the ENL patients studied. Concentrations of TNF above 1000 pg/ml were found in four patients with ENL. Development of erythema multiforme in these ENL patients represented an aggravating factor and all four patients suffering from this type of lesion demonstrated increased serum TNF levels. All BT patients tested presented elevated IL-1 levels, while only half of them presented elevated levels of TNF. No correlation was found between any particular systemic symptoms and the levels of TNF and IL-1. These results suggest that TNF and IL-1 may be implicated in leprosy reactions, either acting directly or in synergism with other cytokines.
journal_name
Clin Exp Immunoljournal_title
Clinical and experimental immunologyauthors
Sarno EN,Grau GE,Vieira LM,Nery JAsubject
Has Abstractpub_date
1991-04-01 00:00:00pages
103-8issue
1eissn
0009-9104issn
1365-2249journal_volume
84pub_type
杂志文章abstract：:Eighteen patients showing hypersensitivity to nitrofurantoin with symptoms like sudden fever, malaise, cough, pleuritis, leucocytosis and sometimes eosinophilia were analysed for immune reactions, i.e. lymphocyte transformation and specific antibodies. In addition, thirty-three individuals treated with the drug but wi...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1977-06-01 00:00:00
abstract：:The repeated administration of a monoclonal anti-Sm antibody (KSml) resulted in a significant prolongation of life in MRL-lpr/lpr lupus mice with a 50% mortality of 36 weeks compared with 18-24 weeks in the control groups. Control animals injected with APC11 (a myeloma protein of the same isotype) lived no longer than...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1986-07-01 00:00:00
abstract：:Specific assays for 5'-nucleotidase, adenosine diphosphatase (ADPase) and Mg2+-dependent adenosine triphosphatase (Mg2+-ATPase) have been optimized for human lymphocytes and their subcellular localizations determined by sucrose density gradient centrifugation. 5'-Nucleotidase was localized solely to the plasma membran...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1982-08-01 00:00:00
abstract：:Recombinant human deoxyribonuclease I (rhDNase) may be an effective therapeutic for the treatment of systemic lupus erythematosus (SLE). The pharmacodynamics of rhDNase in serum was investigated using two activity assays: one based on hydrolysis of a radiolabelled phage DNA and the other based on hydrolysis of human c...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2249.1998.00647.x
更新日期：1998-08-01 00:00:00
abstract：:Blood monocytes of patients with thyroid autoimmune disease (TAID) display defects in rearranging their cortical actomyosin cytoskeleton ('polarize') in response to chemoattractants. Such rearrangements also take place after the adherence of monocytes to the extracellular matrix (ECM). It is therefore not surprising t...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2249.2001.01583.x
更新日期：2001-07-01 00:00:00
abstract：:Tolerogenic dendritic cells (DCs) play a critical role in the induction of regulatory T cells (Tregs ), which in turn suppress effector T cell responses. We have previously shown the induction of DCs from human and mouse monocytic cell lines, mouse splenocytes and human peripheral blood monocytes by a novel apolipopro...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/cei.12370
更新日期：2014-09-01 00:00:00
abstract：:Cyclosporin A (Cy A) enhances immunoglobulin (Ig) production by EB virus stimulated peripheral blood lymphocytes from normal subjects with prior immunity to EB virus. In 11 normal adults, this enhancement of Ig production correlated with T cell-mediated cytotoxic regression. Cultures from normal healthy adults without...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1983-07-01 00:00:00
abstract：:The circulating and cervical B cell responses to Chlamydia trachomatis plasmid protein pgp3 were characterized in children and adults with ocular or genital chlamydial infection using the enzyme-linked immunospot assay (ELISPOT) and ELISA. No pgp3-specific ASCs were detected in healthy controls, but predominantly IgA ...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2249.2003.02163.x
更新日期：2003-06-01 00:00:00
abstract：:In order to define the optimal conditions for detection of microbial antigens in urine, urinary HBsAg excreted during hepatitis B was chosen as a model. Using commercial kits, which mainly involve anti-discontinuous epitopes, we found urinary HBsAg in only 50% of patients with HBsAg in their sera. In contrast, with an...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2249.1992.tb02991.x
更新日期：1992-02-01 00:00:00
abstract：:Regulation of the immune response directed against Leishmania is critical for the establishment of effective control of the disease. It is likely that some types of immune responses directed against Leishmania can lead to more severe clinical forms of leishmaniasis causing a poor control of the pathogen and/or patholo...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2249.2004.02426.x
更新日期：2004-05-01 00:00:00
abstract：:To investigate the possible role of tryptophan metabolism in immune regulation of primary Sjögren's syndrome (pSS) the serum concentrations of tryptophan and its metabolite kynurenine were measured by reverse-phase high-performance liquid chromatography (HPLC) in 103 patients with pSS, 56 patients with sicca symptoms ...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2249.2005.02889.x
更新日期：2005-10-01 00:00:00
abstract：:It has recently been proposed that theophylline activates T suppressor systems in vivo and this evidence is further supported by the ability of the drug to attenuate the allograft rejection in humans and in experimental animals. In this study the acute effects of aminophylline on human peripheral blood lymphocyte (PBL...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1984-09-01 00:00:00
abstract：:Commercial cellular tests are used to diagnose Lyme borreliosis (LB), but studies on their clinical validation are lacking. This study evaluated the utility of an in-house and a commercial enzyme-linked immunosorbent spot (ELISpot) assay for the diagnosis of Lyme neuroborreliosis (LNB). Prospectively, peripheral blood...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/cei.13393
更新日期：2020-03-01 00:00:00
abstract：:Peripheral blood natural killer (NK) activity was analysed from 32 asthma patients and 13 control donors. Male patients and female patients with atopic asthma had significantly stronger NK activity against the leukaemic cell line K-562. Fractionation of patient mononuclear cells by discontinuous density gradient centr...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1985-01-01 00:00:00
abstract：:Atherosclerosis is an inflammatory response, probably to a range of initiating causes. Chronic infection with Chlamydia pneumoniae (C.pn) has been suggested as one cause, but the nature of the association is controversial, in large part due to lack of an identified mechanism to link infection with the atherosclerotic ...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2249.2006.03185.x
更新日期：2006-11-01 00:00:00
abstract：:The Km for AMP for 5' nucleotidase was increased in lymphocytes from patients with common variable (CVH) and sex linked (XLH) hypogammaglobulinaemia and from patients with chronic lymphatic leukaemia (CLL): the Vmax of the latter was low. The kinetic constants for cord blood lymphocytes are similar to adults and the K...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1984-07-01 00:00:00
abstract：:A technique is described which shows how to obtain a suspension of only progressively motile spermatozoa, from semen, by active penetration into an overlying buffer layer. About 60% of the number of motile spermatozoa in the original semen sample can be obtained as an output in the removed buffer layer. With this meth...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1978-01-01 00:00:00
abstract：:In this study, we hypothesized that the granulomatous disorder sarcoidosis is not caused by a single pathogen, but rather results from abnormal responses of Toll-like receptors (TLRs) to conserved bacterial elements. Unsorted bronchoalveolar lavage (BAL) cells from patients with suspected pulmonary sarcoidosis and hea...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/cei.12138
更新日期：2013-09-01 00:00:00
abstract：:Granulocyte colony-stimulating factor (G-CSF) administration in vivo has been shown to improve the defence mechanisms against infection by different microbes. Here we evaluated a possible protective role of this molecule in a mouse model of mycobacterial infection. The administration of recombinant G-CSF promoted an e...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2249.2001.01552.x
更新日期：2001-05-01 00:00:00
abstract：:Activation of the interferon (IFN) system is an early defence mechanism against viral infections. The virus stimulates production of IFN by nucleated cells including the peripheral blood mononuclear cells (PBMC), and this IFN in turn activates several IFN-dependent immune mechanisms including the induction of an anti-...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1985-05-01 00:00:00
abstract：:We previously demonstrated that high levels of IL-6/sIL-6R complexes are present in sera of patients with systemic juvenile idiopathic arthritis (s-JIA) and that the amount of IL-6 estimated in the IL-6/sIL-6R complexes is markedly higher than that measured by the B9 assay. Here, we show that two additional bioassays,...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2249.2003.02052.x
更新日期：2003-02-01 00:00:00
abstract：:Significant levels of EAC-rosette inhibition compared to control subjects were found in the sera of patients with focal and segmental hyalinosis (FSH), membranoproliferative glomerulonephritis (MPGN) and extra-membranous glomerulonephritis (EGN). In patients with IgA disease, although some sera produced high levels of...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1977-12-01 00:00:00
abstract：:Dendritic cells (DCs) were prepared from human bronchoalveolar lavage (BAL) cells. We previously reported that, in particular, the CD1a fraction of the low autofluorescent (LAF) cells contains the precursors for DCs: after overnight culture, 40% of the LAF cells change into functionally and phenotypically prototypic d...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2249.2000.01354.x
更新日期：2000-11-01 00:00:00
abstract：:Analysis of the VH gene repertoire of the J558 family was done in lipopolysaccharide (LPS)-stimulated resting cells and in vivo activated cells derived from C57Bl/6-lpr mice (IghCb). Using a restriction fragment length polymorphism (RFLP) based on digestion with the restriction enzyme Pstl, the expression of the subfa...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2249.1995.tb05543.x
更新日期：1995-02-01 00:00:00
abstract：:An experimental model of immunological deficiency obtained by treating mice for 6 months with serum of human blood drawn from different healthy individuals has been studied. The results show that an alteration of a circulating lymphocyte population with alterations of the ratio CD4+/CD8+ appeared in mice stimulated fo...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2249.1997.tb08315.x
更新日期：1997-11-01 00:00:00
abstract：:Glucocorticoid hormones (GCH) regulate, through the apoptotic process, the negative selection of immature T cells in the thymus. Because apoptosis seems to occur also in the maintenance of peripheral tolerance, we have investigated whether GCH may induce apoptosis in human mature lymphocytes. Peripheral blood lymphocy...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2249.1996.tb08306.x
更新日期：1996-03-01 00:00:00
abstract：:Individuals with type 1 diabetes (T1D) are at increased risk of coeliac disease (CD), autoimmune thyroiditis and autoimmune gastritis, but the absolute risks are unclear. The aim of this study was to investigate the prevalence of autoantibodies to tissue transglutaminase (TGA), thyroid peroxidase (TPOA) and gastric H+...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/cei.13115
更新日期：2018-06-01 00:00:00
abstract：:Complement receptor type 1 (CR1) is an integral membrane protein of many haematopoietic cells and plays an important role in the clearance of complement-associated immune complexes, favouring their transport to liver and spleen macrophages. A small amount of soluble CR1 (sCR1) is also found in plasma and might origina...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2249.1998.00707.x
更新日期：1998-10-01 00:00:00
abstract：:Polyspecific IgG given intravenously at high doses (IVIG) is used for immunomodulatory therapy in autoimmune diseases such as idiopathic thrombocytopenic purpura and myasthenia gravis. It is assumed that the clinical effect is brought about in part by a modulation of mononuclear phagocyte function, in particular by an...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2249.1990.tb05421.x
更新日期：1990-10-01 00:00:00
abstract：:A new, specific haemolytic assay for C2 is described using EAC43 (Antrypol) cells and serum deficient in C4 and C2 from a patient with hereditary angio-oedema. The method is simple and the results in pathological and normal sera correlate well with those obtained using the EA and C2-deficient serum method. ...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1976-09-01 00:00:00
