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HBsAg in urine: a new approach for the detection of urinary antigens.

Abstract：

:In order to define the optimal conditions for detection of microbial antigens in urine, urinary HBsAg excreted during hepatitis B was chosen as a model. Using commercial kits, which mainly involve anti-discontinuous epitopes, we found urinary HBsAg in only 50% of patients with HBsAg in their sera. In contrast, with an inhibition method involving a monoclonal antibody recognizing a continuous epitope, urinary HBsAg was found in 100% of these patients. Structural analysis of HBsAg showed that urinary HBsAg is denatured; it can escape detection by commercial kits well fitted for detection of native serum HBsAg. General implications for the revelation of urinary microbial antigens are discussed.

journal_name

Clin Exp Immunol

journal_title

Clinical and experimental immunology

authors

Badur S,Grangeot-Keros L,Pillot J

doi

10.1111/j.1365-2249.1992.tb02991.x

subject

Has Abstract

pub_date

1992-02-01 00:00:00

pages

298-303

issue

2

eissn

0009-9104

issn

1365-2249

journal_volume

87

pub_type

杂志文章

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