Abstract:
BACKGROUND:SKI and SnoN proteins have been shown to inhibit TGF-β signaling, acting both as transcriptional co-repressors in the cell nucleus, and as sequestrators of SMAD proteins in the cytoplasm. TGF-β, on the other hand, induces rapid, proteasome-mediated, degradation of both proteins. How elevated SKI and SnoN protein levels co-exist with active autocrine TGF-β signaling in cancer cells is yet to be understood. RESULTS:In this study, we found elevated SKI and SnoN protein levels in a panel of melanoma cell lines, as compared to normal melanocytes. There was no correlation between SKI protein content and the capacity of melanoma cells to invade Matrigel™, to form subcutaneous tumors, or to metastasize to bone after intracardiac inoculation into nude mice. Nor did we find a correlation between SKI expression and histopathological staging of human melanoma. TGF-β induced a rapid and dose-dependent degradation of SKI protein, associated with SMAD3/4 specific transcriptional response and induction of pro-metastatic target genes, partially prevented by pharmacologic blockade of proteasome activity. SKI knockdown in 1205Lu melanoma cells did not alter their invasive capacity or transcriptional responses to TGF-β, and did not allow p21 expression in response to TGF-β or reveal any growth inhibitory activity of TGF-β. CONCLUSIONS:Despite high expression in melanoma cells, the role of SKI in melanoma remains elusive: SKI does not efficiently interfere with the pro-oncogenic activities of TGF-β, unless stabilized by proteasome blockade. Its highly labile nature makes it an unlikely target for therapeutic intervention.
journal_name
Mol Cancerjournal_title
Molecular cancerauthors
Javelaud D,van Kempen L,Alexaki VI,Le Scolan E,Luo K,Mauviel Adoi
10.1186/1476-4598-10-2subject
Has Abstractpub_date
2011-01-06 00:00:00pages
2issue
1issn
1476-4598pii
1476-4598-10-2journal_volume
10pub_type
杂志文章相关文献
Molecular Cancer文献大全abstract:BACKGROUND:Fusion of the MOZ and TIF2 genes by an inv (8) (p11q13) translocation has been identified in patients with acute mixed-lineage leukemia. Characterization of the molecular structure of the MOZ-TIF2 fusion protein suggested that the fusion protein would effect on nuclear receptor signaling. RESULTS:A series o...
journal_title:Molecular cancer
pub_type: 杂志文章
doi:10.1186/1476-4598-6-51
更新日期:2007-08-13 00:00:00
abstract:INTRODUCTION:Tumor-initiating cells (TICs), aka "cancer stem cells", are believed to fuel tumors and to sustain therapy resistance and systemic metastasis. Breast cancer is the first human carcinoma in which a subpopulation of cells displaying a specific CD44+/CD24-/low/ESA+ antigenic phenotype was found to have TIC pr...
journal_title:Molecular cancer
pub_type: 杂志文章
doi:10.1186/1476-4598-13-213
更新日期:2014-09-12 00:00:00
abstract::MicroRNAs (miRNAs) are emerging as central players in shaping the biology of the Tumor Microenvironment (TME). They do so both by modulating their expression levels within the different cells of the TME and by being shuttled among different cell populations within exosomes and other extracellular vesicles. This review...
journal_title:Molecular cancer
pub_type: 杂志文章,评审
doi:10.1186/s12943-016-0525-3
更新日期:2016-05-26 00:00:00
abstract:BACKGROUND:The epigenetic regulation of immune response has been demonstrated in recent studies. Nonetheless, potential roles of RNA N6-methyladenosine (m6A) modification in tumor microenvironment (TME) cell infiltration remain unknown. METHODS:We comprehensively evaluated the m6A modification patterns of 1938 gastric...
journal_title:Molecular cancer
pub_type: 杂志文章
doi:10.1186/s12943-020-01170-0
更新日期:2020-03-12 00:00:00
abstract:BACKGROUND:Most ovarian cancers are highly invasive in nature and the high burden of metastatic disease make them a leading cause of mortality among all gynaecological malignancies. The homeodomain transcription factor, PITX2 is associated with cancer in different tissues. Our previous studies demonstrated increased PI...
journal_title:Molecular cancer
pub_type: 杂志文章
doi:10.1186/s12943-015-0433-y
更新日期:2015-08-23 00:00:00
abstract:BACKGROUND:Incorrect segregation of whole chromosomes or parts of chromosome leads to aneuploidy commonly observed in cancer. The correct centrosome duplication, assuring assembly of a bipolar mitotic spindle, is essential for chromosome segregation fidelity and preventing aneuploidy. Alteration of p53 and pRb function...
journal_title:Molecular cancer
pub_type: 杂志文章
doi:10.1186/1476-4598-5-38
更新日期:2006-09-20 00:00:00
abstract::Long-noncoding RNAs (lncRNAs) are a group of transcripts that are longer than 200 nucleotides and do not code for proteins. However, this class of RNAs plays pivotal regulatory roles. The mechanism of their action is highly complex. Mounting evidence shows that lncRNAs can regulate cancer onset and progression in a va...
journal_title:Molecular cancer
pub_type: 杂志文章,评审
doi:10.1186/s12943-017-0699-3
更新日期:2017-07-24 00:00:00
abstract::IGF-1 is a potent mitogen of major importance in the mammary gland. IGF-1 binding to the cognate receptor, IGF-1R, triggers a signaling cascade leading to proliferative and anti-apoptotic events. Although many of the relevant molecular pathways and intracellular cascades remain to be elucidated, a growing body of evid...
journal_title:Molecular cancer
pub_type: 杂志文章,评审
doi:10.1186/s12943-015-0291-7
更新日期:2015-02-15 00:00:00
abstract:BACKGROUND:An organic extract of the recreational herb khat (Catha edulis Forsk.) triggers cell death in various leukemia cell lines in vitro. The chemotherapeutics camptothecin, a plant alkaloid topoisomerase I inhibitor, was tested side-by-side with khat in a panel of acute myeloid leukemia cell lines to elucidate me...
journal_title:Molecular cancer
pub_type: 杂志文章
doi:10.1186/1476-4598-8-101
更新日期:2009-11-13 00:00:00
abstract:BACKGROUND:Salivary Adenoid cystic carcinoma (SACC) patients with local invasion and lung metastasis are often resistant to conventional therapy such as operation, chemotherapy and radiotherapy. To explore the underling mechanisms, we studied the roles of miRNA in regulating invasiveness of SACC cells. METHODS:MicroRN...
journal_title:Molecular cancer
pub_type: 杂志文章
doi:10.1186/s12943-015-0344-y
更新日期:2015-04-29 00:00:00
abstract:BACKGROUND:Hepatocellular carcinoma (HCC) is one of the leading causes of cancer-related death worldwide, and the biology of this cancer remains poorly understood. Recent evidence indicates that long non-coding RNAs (lncRNAs) are found to be dysregulated in a variety of cancers, including HCC. Taurine Up-regulated Gene...
journal_title:Molecular cancer
pub_type: 杂志文章
doi:10.1186/s12943-015-0431-0
更新日期:2015-09-04 00:00:00
abstract:BACKGROUND:Aurora-A is an oncogenic kinase playing well-documented roles in mitotic spindle organisation. We previously found that Aurora-A inactivation yields the formation of spindles with fragmented poles that can drive chromosome mis-segregation. Here we have addressed the mechanism through which Aurora-A activity ...
journal_title:Molecular cancer
pub_type: 杂志文章
doi:10.1186/1476-4598-10-131
更新日期:2011-10-19 00:00:00
abstract:BACKGROUND:Taurine upregulated gene1 (TUG1) as a 7.1-kb lncRNA, has been shown to play an oncogenic role in various cancers. However, the biological functions of lncRNA TUG1 in small cell lung cancer (SCLC) remain unknown. The aim of this study is to explore the roles of TUG1 in cell growth and chemoresistance of SCLC ...
journal_title:Molecular cancer
pub_type: 杂志文章
doi:10.1186/s12943-016-0575-6
更新日期:2017-01-09 00:00:00
abstract:BACKGROUND:Targeted therapies have improved therapeutic options of treating renal cell carcinoma (RCC). However, drug response is temporary due to resistance development. METHODS:Functional and molecular changes in RCC Caki-1 cells, after acquired resistance to the mammalian target of rapamycin (mTOR)-inhibitor everol...
journal_title:Molecular cancer
pub_type: 杂志文章
doi:10.1186/1476-4598-13-152
更新日期:2014-06-16 00:00:00
abstract:INTRODUCTION:Anaplastic thyroid carcinoma, which is one of the most aggressive, malignant tumors in humans, results in an extremely poor prognosis despite chemotherapy and radiotherapy. The present study was designed to evaluate therapeutic effects of radiation by glycerol on p53-mutant anaplastic thyroid carcinoma cel...
journal_title:Molecular cancer
pub_type: 杂志文章
doi:10.1186/1476-4598-1-4
更新日期:2002-10-04 00:00:00
abstract:BACKGROUND:Hypoxia can halt cell cycle progression of several cell types at the G1/S interface. The arrest needs to be overcome by cancer cells. We have previously shown that the hypoxia-inducible cellular oxygen sensor PHD3/EGLN3 enhances hypoxic cell cycle entry at the G1/S boundary. METHODS:We used PHD3 knockdown b...
journal_title:Molecular cancer
pub_type: 杂志文章
doi:10.1186/s12943-015-0410-5
更新日期:2015-07-30 00:00:00
abstract:BACKGROUND:Interferon alpha (IFNα) is a well-established regulator of immunosuppression in head and neck squamous cell carcinoma (HNSCC), while the role of long noncoding RNAs (lncRNAs) in immunosuppression remains largely unknown. METHODS:Differentially expressed lncRNAs were screened under IFNα stimulation using lnc...
journal_title:Molecular cancer
pub_type: 杂志文章
doi:10.1186/s12943-019-1123-y
更新日期:2020-01-06 00:00:00
abstract:BACKGROUND:Triple negative breast cancer (TNBC) remains the most challenging breast cancer subtype so far. Specific therapeutic approaches have rarely achieved clinical improvements in treatment of TNBC patients and effective molecular biomarkers are largely unknown. METHODS:We used paired TNBC samples and high throug...
journal_title:Molecular cancer
pub_type: 杂志文章
doi:10.1186/s12943-020-01259-6
更新日期:2020-09-11 00:00:00
abstract:BACKGROUND:In polymorphonuclear leukocytes (PMNL), mobilization of calcium ions is one of the early events triggered by binding of chemoattractant to its receptors. Besides chemotaxis, a variety of other functional responses are dependent on calcium ion mobilization. PMNL from chronic myeloid leukaemia (CML) patients t...
journal_title:Molecular cancer
pub_type: 杂志文章
doi:10.1186/1476-4598-5-65
更新日期:2006-11-27 00:00:00
abstract:BACKGROUND:In recent years, circular RNAs (circRNAs), a new star of non-coding RNA, have been emerged as vital regulators and gained much attention for involvement of initiation and progression of diverse kinds of human diseases, especially cancer. However, regulatory role, clinical significance and underlying mechanis...
journal_title:Molecular cancer
pub_type: 杂志文章
doi:10.1186/s12943-018-0933-7
更新日期:2019-01-08 00:00:00
abstract:BACKGROUND:Abnormal amplification/expression of HER-2/neu oncogene has been causally linked with tumorigenesis and metastasis in breast cancer and associated with shortened overall survival of patients. Recently, heat shock protein 27 (Hsp27) was reported to be highly expressed in HER-2/neu positive tumors and cell lin...
journal_title:Molecular cancer
pub_type: 杂志文章
doi:10.1186/1476-4598-6-52
更新日期:2007-08-14 00:00:00
abstract:BACKGROUND:microRNAs (miRNAs) play both oncogenic and oncostatic roles in leukemia. However, the molecular details underlying miRNA-mediated regulation of their target genes in pediatric B- and T-cell acute lymphoblastic leukemias (ALLs) remain unclear. The present study investigated the relationship between miR-2909 a...
journal_title:Molecular cancer
pub_type: 杂志文章
doi:10.1186/1476-4598-13-175
更新日期:2014-07-18 00:00:00
abstract::Because circulating microRNAs (miRNAs) have drawn a great deal of attention as promising novel cancer diagnostics and prognostic biomarkers, we sought to identify serum miRNAs significantly associated with outcome in glioblastoma patients. To do this, we performed global miRNA profiling in serum samples from 106 prima...
journal_title:Molecular cancer
pub_type: 信件
doi:10.1186/s12943-017-0628-5
更新日期:2017-03-11 00:00:00
abstract::Bortezomib is a novel anti-cancer agent which has shown promising activity in non-small lung cancer (NSCLC) patients. However, only a subset of patients respond to this treatment. We show that NSCLC cell lines are differentially sensitive to bortezomib, IC50 values ranging from 5 to 83 nM. The apoptosis-inducing poten...
journal_title:Molecular cancer
pub_type: 杂志文章
doi:10.1186/1476-4598-6-73
更新日期:2007-11-17 00:00:00
abstract:BACKGROUND:SOX2, a high mobility group (HMG)-box containing transcription factor, is a key regulator during development of the nervous system and a persistent marker of neural stem cells. Recent studies suggested a role of SOX2 in tumor progression. In our previous work we detected SOX2 in glioma cells and glioblastoma...
journal_title:Molecular cancer
pub_type: 杂志文章
doi:10.1186/1476-4598-10-137
更新日期:2011-11-09 00:00:00
abstract:BACKGROUND:Hodgkin lymphoma (HL) and Anaplastic Large Cell Lymphoma (ALCL), are forms of malignant lymphoma defined by unique morphologic, immunophenotypic, genotypic, and clinical characteristics, but both overexpress CD30. We used sub-megabase resolution tiling (SMRT) array-based comparative genomic hybridization to ...
journal_title:Molecular cancer
pub_type: 杂志文章
doi:10.1186/1476-4598-7-2
更新日期:2008-01-07 00:00:00
abstract::In recent years, thyroid malignances have become more prevalent, especially among women. The most common sporadic types of thyroid tumors of follicular origin include papillary, follicular and anaplastic thyroid carcinomas. Although modern diagnosis methods enable the identification of tumors of small diameter, tumor ...
journal_title:Molecular cancer
pub_type: 杂志文章,评审
doi:10.1186/s12943-018-0866-1
更新日期:2018-08-08 00:00:00
abstract::Cancer research has mainly focused on alterations of genes and proteins in cancer cells themselves that result in either gain-of-function in oncogenes or loss-of-function in tumour-suppressor genes. However, stromal variables within or around tumours, including blood and lymph vessels, stromal cells and various protei...
journal_title:Molecular cancer
pub_type: 杂志文章,评审
doi:10.1186/1476-4598-5-43
更新日期:2006-10-06 00:00:00
abstract:BACKGROUND:Cancer/testis (CT) genes have expression normally restricted to the testis, but become activated during oncogenesis, so they have excellent potential as cancer-specific biomarkers. Evidence is starting to emerge to indicate that they also provide function(s) in the oncogenic programme. Human TEX19 is a recen...
journal_title:Molecular cancer
pub_type: 杂志文章
doi:10.1186/s12943-017-0653-4
更新日期:2017-04-26 00:00:00
abstract:BACKGROUND:The poor prognosis of esophageal squamous cell carcinoma (ESCC) highlights the need for novel strategies against this disease. Our previous study suggested the involvement of CCL2 and tumor associated macrophages (TAMs) in esophageal carcinogenesis. Despite the recognition of TAMs as a promising target for c...
journal_title:Molecular cancer
pub_type: 杂志文章
doi:10.1186/s12943-020-01165-x
更新日期:2020-02-27 00:00:00