Abstract:
BACKGROUND:Cancer/testis (CT) genes have expression normally restricted to the testis, but become activated during oncogenesis, so they have excellent potential as cancer-specific biomarkers. Evidence is starting to emerge to indicate that they also provide function(s) in the oncogenic programme. Human TEX19 is a recently identified CT gene, but a functional role for TEX19 in cancer has not yet been defined. METHODS:siRNA was used to deplete TEX19 levels in various cancer cell lines. This was extended using shRNA to deplete TEX19 in vivo. Western blotting, fluorescence activated cell sorting and immunofluorescence were used to study the effect of TEX19 depletion in cancer cells and to localize TEX19 in normal testis and cancer cells/tissues. RT-qPCR and RNA sequencing were employed to determine the changes to the transcriptome of cancer cells depleted for TEX19 and Kaplan-Meier plots were generated to explore the relationship between TEX19 expression and prognosis for a range of cancer types. RESULTS:Depletion of TEX19 levels in a range of cancer cell lines in vitro and in vivo restricts cellular proliferation/self-renewal/reduces tumour volume, indicating TEX19 is required for cancer cell proliferative/self-renewal potential. Analysis of cells depleted for TEX19 indicates they enter a quiescent-like state and have subtle defects in S-phase progression. TEX19 is present in both the nucleus and cytoplasm in both cancerous cells and normal testis. In cancer cells, localization switches in a context-dependent fashion. Transcriptome analysis of TEX19 depleted cells reveals altered transcript levels of a number of cancer-/proliferation-associated genes, suggesting that TEX19 could control oncogenic proliferation via a transcript/transcription regulation pathway. Finally, overall survival analysis of high verses low TEX19 expressing tumours indicates that TEX19 expression is linked to prognostic outcomes in different tumour types. CONCLUSIONS:TEX19 is required to drive cell proliferation in a range of cancer cell types, possibly mediated via an oncogenic transcript regulation mechanism. TEX19 expression is linked to a poor prognosis for some cancers and collectively these findings indicate that not only can TEX19 expression serve as a novel cancer biomarker, but may also offer a cancer-specific therapeutic target with broad spectrum potential.
journal_name
Mol Cancerjournal_title
Molecular cancerauthors
Planells-Palop V,Hazazi A,Feichtinger J,Jezkova J,Thallinger G,Alsiwiehri NO,Almutairi M,Parry L,Wakeman JA,McFarlane RJdoi
10.1186/s12943-017-0653-4subject
Has Abstractpub_date
2017-04-26 00:00:00pages
84issue
1issn
1476-4598pii
10.1186/s12943-017-0653-4journal_volume
16pub_type
杂志文章相关文献
Molecular Cancer文献大全abstract:BACKGROUND:Perturbation of DNA methylation is frequent in cancers and has emerged as an important mechanism involved in tumorigenesis. To determine how DNA methylation is modified in the genome of primary glioma, we used Methyl-DNA immunoprecipitation (MeDIP) and Nimblegen CpG promoter microarrays to identify different...
journal_title:Molecular cancer
pub_type: 杂志文章
doi:10.1186/1476-4598-10-124
更新日期:2011-09-30 00:00:00
abstract:BACKGROUND:Esophageal squamous cell carcinoma (ESCC) is one of the most lethal malignancies. Neovascularization during tumorigenesis supplies oxygen and nutrients to proliferative tumor cells, and serves as a conduit for migration. Targeting oncogenes involved in angiogenesis is needed to treat organ-confined and local...
journal_title:Molecular cancer
pub_type: 杂志文章
doi:10.1186/s12943-018-0930-x
更新日期:2019-01-04 00:00:00
abstract:BACKGROUND:Pancreatic cancer is a highly lethal disease and has the worst prognosis of any major malignancy. G protein-coupled receptor GPR87 is reported to be overexpressed in multiple cancers. The clinical significance and biological role of GPR87 in pancreatic cancer, however, remain to be established. METHODS:GPR8...
journal_title:Molecular cancer
pub_type: 杂志文章
doi:10.1186/s12943-017-0627-6
更新日期:2017-03-14 00:00:00
abstract::Chimeric antigen receptor T (CAR-T) cell therapy is an emerging and effective cancer immunotherapy. Especially in hematological malignancies, CAR-T cells have achieved exciting results. Two Anti-CD19 CAR-T therapies have been approved for the treatment of CD19-positive leukemia or lymphoma. However, the application of...
journal_title:Molecular cancer
pub_type: 杂志文章,评审
doi:10.1186/s12943-019-1057-4
更新日期:2019-08-20 00:00:00
abstract:BACKGROUND:Triple negative breast cancer (TNBC) remains the most challenging breast cancer subtype so far. Specific therapeutic approaches have rarely achieved clinical improvements in treatment of TNBC patients and effective molecular biomarkers are largely unknown. METHODS:We used paired TNBC samples and high throug...
journal_title:Molecular cancer
pub_type: 杂志文章
doi:10.1186/s12943-020-01259-6
更新日期:2020-09-11 00:00:00
abstract:BACKGROUND:Although both circular RNAs (circRNAs) and autophagy are associated with the function of breast cancer (BC), whether circRNAs regulate BC progression via autophagy remains unknown. In this study, we aim to explore the regulatory mechanisms and the clinical significance of autophagy-associated circRNAs in BC....
journal_title:Molecular cancer
pub_type: 杂志文章
doi:10.1186/s12943-020-01152-2
更新日期:2020-03-25 00:00:00
abstract:BACKGROUND:Long non-coding RNAs (lncRNAs) have been identified as important epigenetic regulators that play critical roles in human cancers. However, the regulatory functions of lncRNAs in tumorigenesis remains to be elucidated. Here, we aimed to investigate the molecular mechanisms and potential clinical application o...
journal_title:Molecular cancer
pub_type: 杂志文章
doi:10.1186/s12943-020-01232-3
更新日期:2020-07-15 00:00:00
abstract:BACKGROUND:Colorectal cancer is third most common malignancy and is the second most common cause of cancer-related death. The MUC1 heterodimeric protein is aberrantly overexpressed in colorectal cancer and has been linked to poor outcomes in this disease. Here, we investigate the effects of the MUC1-C subunit inhibitor...
journal_title:Molecular cancer
pub_type: 杂志文章
doi:10.1186/s12943-017-0608-9
更新日期:2017-02-02 00:00:00
abstract:BACKGROUND:In contrast to tumor-suppressive apoptosis and autophagic cell death, necrosis promotes tumor progression by releasing the pro-inflammatory and tumor-promoting cytokine high mobility group box 1 (HMGB1), and its presence in tumor patients is associated with poor prognosis. Thus, necrosis has important clinic...
journal_title:Molecular cancer
pub_type: 杂志文章
doi:10.1186/1476-4598-10-113
更新日期:2011-09-14 00:00:00
abstract:BACKGROUND:Nilotinib is a tyrosine kinase inhibitor with high target specificity. Here, we characterized the effects of nilotinib for the first time on CD4+CD25+ regulatory T cells (Tregs) which regulate anti-tumor/leukemia immune responses. DESIGN AND METHODS:Carboxyfluorescein diacetate succinimidyl ester (CFSE) and...
journal_title:Molecular cancer
pub_type: 杂志文章
doi:10.1186/1476-4598-9-22
更新日期:2010-01-29 00:00:00
abstract::Autophagy is a highly conserved catabolic process that mediates degradation of pernicious or dysfunctional cellular components, such as invasive pathogens, senescent proteins, and organelles. It can promote or suppress tumor development, so it is a "double-edged sword" in tumors that depends on the cell and tissue typ...
journal_title:Molecular cancer
pub_type: 杂志文章,评审
doi:10.1186/s12943-019-1030-2
更新日期:2019-05-24 00:00:00
abstract:BACKGROUND:Metastasis accounts for the majority of deaths from cancer. Although tumor microenvironment has been shown to have a significant impact on the initiation and/or promotion of metastasis, the mechanism remains elusive. We previously reported that HCT-8 colon cancer cells underwent a phenotypic transition from ...
journal_title:Molecular cancer
pub_type: 杂志文章
doi:10.1186/1476-4598-13-131
更新日期:2014-05-29 00:00:00
abstract:BACKGROUND:TPC-1 is a papillary thyroid carcinoma (PTC)-derived cell line that spontaneously expresses the oncogene RET/PTC1. TPC-1 treated with the RET/PTC1 inhibitor RPI-1 displayed a cytostatic and reversible inhibition of cell proliferation and a strong activation of focal adhesion kinase (FAK). As dasatinib inhibi...
journal_title:Molecular cancer
pub_type: 杂志文章
doi:10.1186/1476-4598-9-278
更新日期:2010-10-18 00:00:00
abstract:BACKGROUND:Abnormal amplification/expression of HER-2/neu oncogene has been causally linked with tumorigenesis and metastasis in breast cancer and associated with shortened overall survival of patients. Recently, heat shock protein 27 (Hsp27) was reported to be highly expressed in HER-2/neu positive tumors and cell lin...
journal_title:Molecular cancer
pub_type: 杂志文章
doi:10.1186/1476-4598-6-52
更新日期:2007-08-14 00:00:00
abstract::This article has been retracted. Please see the retraction notice for more detail: https://doi.org/10.1186/s12943-015-0389-y. ...
journal_title:Molecular cancer
pub_type: 杂志文章,撤回出版物
doi:10.1186/s12943-020-01242-1
更新日期:2020-08-15 00:00:00
abstract:BACKGROUND:N6-methyladenosine (m6A) emerges as one of the most important modification of RNA. Bladder cancer is a common cancer type in developed countries, and hundreds of thousands of bladder cancer patients die every year. MATERIALS AND METHODS:There are various cells in bladder tumor bulk, and a small population c...
journal_title:Molecular cancer
pub_type: 杂志文章
doi:10.1186/s12943-019-1084-1
更新日期:2019-11-25 00:00:00
abstract::Inflammasomes are large intracellular multi-protein signalling complexes that are formed in the cytosolic compartment as an inflammatory immune response to endogenous danger signals. The formation of the inflammasome enables activation of an inflammatory protease caspase-1, pyroptosis initiation with the subsequent cl...
journal_title:Molecular cancer
pub_type: 杂志文章,评审
doi:10.1186/s12943-018-0900-3
更新日期:2018-11-17 00:00:00
abstract:BACKGROUND:The aberrant expression of microRNA-140-5p (miR-140-5p) has been described in gastric cancer (GC). However, the role of miR-140-5p in GC remains unclear. In this study, the prognostic relevance of miR-140-5p in GC was investigated and YES1 was identified as a novel target of miR-140-5p in regulating tumor pr...
journal_title:Molecular cancer
pub_type: 杂志文章
doi:10.1186/s12943-017-0708-6
更新日期:2017-08-17 00:00:00
abstract::Infection with Human papillomaviruses (HPVs) leads to the development of a wide-range of cancers, accounting for 5% of all human cancers. A prominent example is cervical cancer, one of the leading causes of cancer death in women worldwide. It has been well established that tumor development and progression induced by ...
journal_title:Molecular cancer
pub_type: 杂志文章,评审
doi:10.1186/s12943-015-0361-x
更新日期:2015-04-17 00:00:00
abstract:BACKGROUND:MUC16 is a cell surface mucin expressed at high levels by epithelial ovarian tumors. Following proteolytic cleavage, cell surface MUC16 (csMUC16) is shed in the extracellular milieu and is detected in the serum of cancer patients as the tumor marker CA125. csMUC16 acts as an adhesion molecule and facilitates...
journal_title:Molecular cancer
pub_type: 杂志文章
doi:10.1186/1476-4598-9-118
更新日期:2010-05-24 00:00:00
abstract:BACKGROUND:The disruption of normal gene regulation due to microRNA dysfunction is a common event in cancer pathogenesis. MicroRNA-27b is an example of an oncogenic miRNA, and it is frequently upregulated in breast cancer. MicroRNAs have been found to deregulate tumor metabolism, which typically manifests as heightened...
journal_title:Molecular cancer
pub_type: 杂志文章
doi:10.1186/s12943-018-0851-8
更新日期:2018-07-16 00:00:00
abstract::Recent advances in diagnosis and treatment are enabling a more targeted approach to treating lung cancers. Therapy targeting the specific oncogenic driver mutation could inhibit tumor progression and provide a favorable prognosis in clinical practice. Activating mutations of epidermal growth factor receptor (EGFR) in ...
journal_title:Molecular cancer
pub_type: 杂志文章,评审
doi:10.1186/s12943-018-0777-1
更新日期:2018-02-19 00:00:00
abstract:BACKGROUND:New blood vessel formation, or angiogenic switch, is an essential event in the development of solid tumors and their metastatic growth. Tumor blood vessel formation and remodeling is a complex and multi-step processes. The differentiation and recruitment of mural cells including vascular smooth muscle cells ...
journal_title:Molecular cancer
pub_type: 杂志文章
doi:10.1186/1476-4598-9-209
更新日期:2010-08-05 00:00:00
abstract:BACKGROUND:Triple-negative breast cancer (TNBC) is highly invasive and aggressive and lacks specific molecular targets to improve the prognosis. MiR-25-3p promotes proliferation of many tumors and its role and underlying mechanisms in TNBC remain to be well elucidated. METHODS:Differential expression of miR-25-3p in T...
journal_title:Molecular cancer
pub_type: 杂志文章
doi:10.1186/s12943-017-0754-0
更新日期:2018-01-08 00:00:00
abstract:BACKGROUND:The clinical efficacy of camptothecin (CPT), a drug specifically targeting topoisomerase I (TopoI), is under evaluation for the treatment of malignant gliomas. Due to the high unresponsiveness of these tumours to chemotherapy, it would be very important to study the signalling network that drives camptotheci...
journal_title:Molecular cancer
pub_type: 杂志文章
doi:10.1186/1476-4598-7-66
更新日期:2008-08-11 00:00:00
abstract:BACKGROUND:Adrenocortical carcinoma (ACC) is a rare endocrine malignancy with high mutational heterogeneity and a generally poor clinical outcome. Despite implicated roles of deregulated TP53, IGF-2 and Wnt signaling pathways, a clear genetic association or unique mutational link to the disease is still missing. Recent...
journal_title:Molecular cancer
pub_type: 杂志文章
doi:10.1186/1476-4598-12-87
更新日期:2013-08-05 00:00:00
abstract:BACKGROUND:miR-139-5p was identified to be significantly down-regulated in colon tumor tissues by miRNA array. We aimed to clarify its biological function, molecular mechanisms and direct target gene in colorectal cancer (CRC). METHODS:The biological function of miR-139-5p was examined by cell growth, cell cycle and a...
journal_title:Molecular cancer
pub_type: 杂志文章
doi:10.1186/1476-4598-13-124
更新日期:2014-05-26 00:00:00
abstract:INTRODUCTION:Tumor-initiating cells (TICs), aka "cancer stem cells", are believed to fuel tumors and to sustain therapy resistance and systemic metastasis. Breast cancer is the first human carcinoma in which a subpopulation of cells displaying a specific CD44+/CD24-/low/ESA+ antigenic phenotype was found to have TIC pr...
journal_title:Molecular cancer
pub_type: 杂志文章
doi:10.1186/1476-4598-13-213
更新日期:2014-09-12 00:00:00
abstract:BACKGROUND:CADM1 encodes an immunoglobulin superfamily (IGSF) cell adhesion molecule. Inactivation of CADM1, either by promoter hypermethylation or loss of heterozygosity, has been reported in a wide variety of tumor types, thus it has been postulated as a tumor suppressor gene. FINDINGS:We show for the first time tha...
journal_title:Molecular cancer
pub_type: 杂志文章
doi:10.1186/1476-4598-11-29
更新日期:2012-05-03 00:00:00
abstract:BACKGROUND:Though esophageal cancer is three to four times more common among males than females worldwide, this type of cancer still ranks in the top incidence among women, even more than the female specific cancer types. The occurrence is currently attributed to extrinsic factors, including tobacco use and alcohol con...
journal_title:Molecular cancer
pub_type: 杂志文章
doi:10.1186/s12943-019-0949-7
更新日期:2019-02-08 00:00:00