Glycerol as a chemical chaperone enhances radiation-induced apoptosis in anaplastic thyroid carcinoma cells.


INTRODUCTION:Anaplastic thyroid carcinoma, which is one of the most aggressive, malignant tumors in humans, results in an extremely poor prognosis despite chemotherapy and radiotherapy. The present study was designed to evaluate therapeutic effects of radiation by glycerol on p53-mutant anaplastic thyroid carcinoma cells (8305c cells). To examine the effectiveness of glycerol in radiation induced lethality for anaplastic thyroid carcinoma 8305c cells, we performed colony formation assay and apoptosis analysis. RESULTS:Apoptosis was analyzed with Hoechst 33342 staining and DNA ladder formation assay. 8305c cells became radiosensitive when glycerol was added to culture medium before X-ray irradiation. Apoptosis was induced by X-rays in the presence of glycerol. However, there was little apoptosis induced by X-ray irradiation or glycerol alone. The binding activity of whole cell extracts to bax promoter region was induced by X-rays in the presence of glycerol but not by X-rays alone. CONCLUSION:These findings suggest that glycerol is effective against radiotherapy of p53-mutant thyroid carcinomas.


Mol Cancer


Molecular cancer


Ohnishi K,Ota I,Yane K,Takahashi A,Yuki K,Emoto M,Hosoi H,Ohnishi T





Has Abstract


2002-10-04 00:00:00








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    abstract:BACKGROUND:The disruption of normal gene regulation due to microRNA dysfunction is a common event in cancer pathogenesis. MicroRNA-27b is an example of an oncogenic miRNA, and it is frequently upregulated in breast cancer. MicroRNAs have been found to deregulate tumor metabolism, which typically manifests as heightened...

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    pub_type: 杂志文章


    authors: Eastlack SC,Dong S,Ivan C,Alahari SK

    更新日期:2018-07-16 00:00:00

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    abstract:BACKGROUND:The epithelial-to-mesenchymal transition (EMT) process results in a loss of cell-cell adhesion, increased cell mobility, and is crucial for enabling the metastasis of cancer cells. Recently, the enzyme SIRT1 has been implicated in a variety of physiological processes; however, its role in regulating oral can...

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  • Lung adenocarcinoma-intrinsic GBE1 signaling inhibits anti-tumor immunity.

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  • Tumor-stromal crosstalk in pancreatic cancer and tissue fibrosis.

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    pub_type: 杂志文章,评审


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    abstract:BACKGROUND:The human genome encodes thousands of unique long non-coding RNAs (lncRNAs), and these transcripts are emerging as critical regulators of gene expression and cell fate. However, the transcriptional regulation of their expression is not fully understood. The pivotal transcription factor E2F1 which can induce ...

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    abstract:BACKGROUND:Abnormal amplification/expression of HER-2/neu oncogene has been causally linked with tumorigenesis and metastasis in breast cancer and associated with shortened overall survival of patients. Recently, heat shock protein 27 (Hsp27) was reported to be highly expressed in HER-2/neu positive tumors and cell lin...

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  • Hypoxia inducible prolyl hydroxylase PHD3 maintains carcinoma cell growth by decreasing the stability of p27.

    abstract:BACKGROUND:Hypoxia can halt cell cycle progression of several cell types at the G1/S interface. The arrest needs to be overcome by cancer cells. We have previously shown that the hypoxia-inducible cellular oxygen sensor PHD3/EGLN3 enhances hypoxic cell cycle entry at the G1/S boundary. METHODS:We used PHD3 knockdown b...

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    pub_type: 杂志文章


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    更新日期:2007-06-12 00:00:00

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    abstract:BACKGROUND:The clinical use of BRAF inhibitors for treatment of metastatic melanoma is limited by the development of drug resistance. In this study we investigated whether co-targeting the MAPK and the PI3K-AKT pathway can prevent emergence of resistance or provide additional growth inhibitory effects in vitro. METHOD...

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    pub_type: 杂志文章


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    journal_title:Molecular cancer

    pub_type: 杂志文章


    authors: Kracklauer MP,Schmidt C

    更新日期:2003-11-05 00:00:00

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    pub_type: 杂志文章,评审


    authors: Khaidakov M,Shmookler Reis RJ

    更新日期:2005-09-13 00:00:00

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    authors: Console GM,Herenu CB,Camihort GA,Luna GC,Bracamonte MI,Morel GR,Goya RG

    更新日期:2008-01-25 00:00:00

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    abstract:BACKGROUND:Tumor spreading is the major threat for cancer patients. The recently published anti-cancer drug salinomycin raised hope for an improved treatment by targeting therapy-refractory cancer stem cells. However, an unambiguous role of salinomycin against cancer cell migration and metastasis formation remains elus...

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    journal_title:Molecular cancer

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    pub_type: 杂志文章,评审


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    journal_title:Molecular cancer

    pub_type: 杂志文章


    authors: Wu Q,Hoffmann MJ,Hartmann FH,Schulz WA

    更新日期:2005-05-05 00:00:00

  • Mechanisms of osteopontin and CD44 as metastatic principles in prostate cancer cells.

    abstract:BACKGROUND:The expression level of osteopontin correlates with the metastatic potential of several tumors. Osteopontin is a well-characterized ligand for the alphavbeta3 integrin. The present study was undertaken to elucidate the possible role of osteopontin/alphavbeta3 signaling in prostate cancer cell migration. RES...

    journal_title:Molecular cancer

    pub_type: 杂志文章


    authors: Desai B,Rogers MJ,Chellaiah MA

    更新日期:2007-03-07 00:00:00

  • ISL-1 is overexpressed in non-Hodgkin lymphoma and promotes lymphoma cell proliferation by forming a p-STAT3/p-c-Jun/ISL-1 complex.

    abstract:BACKGROUND:Insulin enhancer binding protein-1 (ISL-1), a LIM-homeodomain transcription factor, is essential for the heart, motor neuron and pancreas development. Recently, ISL-1 has been found in some types of human cancers. However, how ISL-1 exerts the role in tumor development is not clear. METHODS AND RESULTS:The ...

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    pub_type: 杂志文章


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    更新日期:2014-07-29 00:00:00

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    abstract:BACKGROUND:Alterations of chromosome 8 and hypomethylation of LINE-1 retrotransposons are common alterations in advanced prostate carcinoma. In a former study including many metastatic cases, they strongly correlated with each other. To elucidate a possible interaction between the two alterations, we investigated their...

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    pub_type: 杂志文章


    authors: Schulz WA,Alexa A,Jung V,Hader C,Hoffmann MJ,Yamanaka M,Fritzsche S,Wlazlinski A,Müller M,Lengauer T,Engers R,Florl AR,Wullich B,Rahnenführer J

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  • LMP1-augmented kappa intron enhancer activity contributes to upregulation expression of Ig kappa light chain via NF-kappaB and AP-1 pathways in nasopharyngeal carcinoma cells.

    abstract:BACKGROUND:Expression of kappa gene is under the control of distinct cis-regulatory elements, including the kappa intron enhancer (iE kappa) and the kappa 3' enhancer (3'E kappa). The active enhancers and expression of immunoglobulin is generally considered to be restricted to B lymphocytes. However, accumulating evide...

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    pub_type: 杂志文章


    authors: Liu H,Zheng H,Duan Z,Hu D,Li M,Liu S,Li Z,Deng X,Wang Z,Tang M,Shi Y,Yi W,Cao Y

    更新日期:2009-10-27 00:00:00

  • PIWI-interacting RNA-36712 restrains breast cancer progression and chemoresistance by interaction with SEPW1 pseudogene SEPW1P RNA.

    abstract:BACKGROUND:Breast cancer is one of the most common malignancies and the major cause of cancer-related death in women. Although the importance of PIWI-interacting RNAs (piRNAs) in cancer has been increasingly recognized, few studies have been explored the functional mechanism of piRNAs in breast cancer development and p...

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    pub_type: 杂志文章


    authors: Tan L,Mai D,Zhang B,Jiang X,Zhang J,Bai R,Ye Y,Li M,Pan L,Su J,Zheng Y,Liu Z,Zuo Z,Zhao Q,Li X,Huang X,Yang J,Tan W,Zheng J,Lin D

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    abstract:BACKGROUND:Neuroblastoma is responsible for 15% of all childhood cancer deaths. Despite advances in treatment and disease management, the overall 5-year survival rates remain poor in high-risk disease (25-40%). MiR-497 was previously identified by our laboratory as a member of a miRNA expression signature, predictive o...

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    pub_type: 杂志文章


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    abstract:BACKGROUND:Sorafenib, a kinase inhibitor active against various solid tumours, induces oxidative stress and ferroptosis, a new form of oxidative necrosis, in some cancer cells. Clinically-applicable biomarkers that reflect the impact of sorafenib on the redox metabolism of cancer cells are lacking. METHODS:We used gen...

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    pub_type: 杂志文章


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    更新日期:2016-05-16 00:00:00

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    abstract:BACKGROUND:Lung cancer in nonsmokers tends to be driven by a single somatic mutation or a gene fusion. KIF5B-RET fusion is an oncogene identified in non-small cell lung cancers. In this study, we verified the oncogenic activity of KIF5B-RET fusion and investigated how KIF5B-RET activates the specific signaling pathways...

    journal_title:Molecular cancer

    pub_type: 杂志文章


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    abstract:BACKGROUND:Aberrant expression of microRNAs in different human cancer types has been widely reported. MiR-218 acts as a tumor suppressor in diverse human cancer types impacting regulation of multiple genes in oncogenic pathways. Here, we evaluated the expression and function of miR-218 in human lung cancer and ALDH pos...

    journal_title:Molecular cancer

    pub_type: 杂志文章


    authors: Yang Y,Ding L,Hu Q,Xia J,Sun J,Wang X,Xiong H,Gurbani D,Li L,Liu Y,Liu A

    更新日期:2017-08-22 00:00:00

  • Choosing the right cell line for renal cell cancer research.

    abstract::Cell lines are still a tool of choice for many fields of biomedical research, including oncology. Although cancer is a very complex disease, many discoveries have been made using monocultures of established cell lines. Therefore, the proper use of in vitro models is crucial to enhance our understanding of cancer. Ther...

    journal_title:Molecular cancer

    pub_type: 杂志文章,评审


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    更新日期:2016-12-19 00:00:00

  • Functional studies of BCL11A: characterization of the conserved BCL11A-XL splice variant and its interaction with BCL6 in nuclear paraspeckles of germinal center B cells.

    abstract:BACKGROUND:Chromosomal aberrations of BCL11A at 2p16.1 have been reported in a variety of B-cell malignancies and its deficiency in mice leads to a profound block in B-cell development. RESULTS:Alternative pre-mRNA splicing of BCL11A produces multiple isoforms sharing a common N-terminus. The most abundant isoform we ...

    journal_title:Molecular cancer

    pub_type: 杂志文章


    authors: Liu H,Ippolito GC,Wall JK,Niu T,Probst L,Lee BS,Pulford K,Banham AH,Stockwin L,Shaffer AL,Staudt LM,Das C,Dyer MJ,Tucker PW

    更新日期:2006-05-16 00:00:00

  • The Bcl-2/xL inhibitor ABT-263 increases the stability of Mcl-1 mRNA and protein in hepatocellular carcinoma cells.

    abstract:BACKGROUND:Hepatocellular carcinoma (HCC) is one of the major causes of mortality. ABT-263 is a newly synthesized, orally available Bcl-2/xL inhibitor that shows promising efficacy in HCC therapy. ABT-263 inhibits the anti-apoptotic activity of Bcl-2 and Bcl-xL, but not Mcl-1. Previous reports have shown that ABT-263 u...

    journal_title:Molecular cancer

    pub_type: 杂志文章


    authors: Wang B,Ni Z,Dai X,Qin L,Li X,Xu L,Lian J,He F

    更新日期:2014-04-30 00:00:00