Abstract：
:Coxsackievirus B3 (CVB3) is a major pathogen for acute and chronic viral myocarditis. The aim of this study was to investigate the antiviral effects of sophoridine, an alkaloid extracted from Chinese medicinal herb, Sophora flavescens, against CVB3, and the underlying pharmacokinetics. First, we determined the antiviral effects of sophoridine against CVB3 in in vitro (primarily cultured myocardial cells), in vivo (BALB/c mice) and serum pharmacological experiments. Then, we determined the pharmacokinetic behavior in serum samples of SD rats after oral administration by HPLC. Finally, we determined the effects of sophoridine on the production of cytokines in a murine viral myocarditis model by measuring mRNA expression of some important cytokines in hearts of infected BALB/c mice by RT-PCR. We found that sophoridine exhibited obvious antiviral effects both in vitro and in vivo, and serum samples obtained from rats with oral administration of sophoridine reduced the virus titers in infected myocardial cells. The serum concentration profile correlated closely with antiviral activity profile. Moreover, sophoridine significantly enhanced mRNA expression of IL-10 and IFN-gamma, but decreased TNF-alpha mRNA expression. In conclusion, sophoridine possesses antiviral activities against CVB3, by regulating cytokine expression, and it is likely that sophoridine itself, not its metabolites, is mainly responsible for the antiviral activities. Therefore, sophoridine may represent a potential therapeutic agent for viral myocarditis.
journal_name
Life Scijournal_title
Life sciencesauthors
Zhang Y,Zhu H,Ye G,Huang C,Yang Y,Chen R,Yu Y,Cui Xdoi
10.1016/j.lfs.2005.09.034subject
Has Abstractpub_date
2006-03-20 00:00:00pages
1998-2005issue
17eissn
0024-3205issn
1879-0631pii
S0024-3205(05)01069-6journal_volume
78pub_type
杂志文章相关文献
LIFE SCIENCES文献大全abstract：:In the biosynthesis of adrenomedullin (AM), glycine-extended AM, an intermediate form (iAM) processed from proAM is converted to AM[1-52]-NH2, the bioactive mature form of AM (mAM), by enzymatic amidation. We earlier showed that both molecular forms of AM circulate in human plasma. In the present study, to investigate...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(99)00208-8
更新日期：1999-01-01 00:00:00
abstract：:The effects of methadone and morphine on fetal behavior were studied following their administration to either the mother (5micrograms/kg/min) or fetus (0.5micrograms/kg maternal wt./min) using the chronically-instrumented pregnant ewe model. Fourteen unanesthetized, unrestrained pregnant ewes between 130-141 days gest...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(83)90584-2
更新日期：1983-01-01 00:00:00
abstract：AIMS:Toll-like receptor (TLR)9 ligand CpG-oligodeoxynucleotide (CpG-ODN) exerts preconditioning in myocardial ischemia/reperfusion. We hypothesized a postconditioning effect of CpG-ODN in a murine closed-chest model of myocardial infarction. MATERIALS AND METHODS:C57BL/6 (12 weeks, male, WT) mice were instrumented at ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2014.09.029
更新日期：2014-12-05 00:00:00
abstract：:Hemi-pituitary glands of ovariectomized rats were superfused for 4 h with either LHRH or the analog buserelin (HOE 766) at several concentrations, and thereafter with medium only for another 1.5 h. In a further experiment glands were exposed for 2.5 h to LHRH or buserelin at a single concentration (5 ng/ml) and subseq...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(86)90524-2
更新日期：1986-08-04 00:00:00
abstract：AIMS:Circular RNAs (circRNAs) are relevant to atherosclerosis progression. However, the role and mechanism of circRNA hsa_circ_0029589 (circ_0029589) in atherosclerosis are not fully understood. This research aims to explore the function and mechanism of circ_0029589 in oxidized low-density lipoprotein (ox-LDL)-caused ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2020.118251
更新日期：2020-10-15 00:00:00
abstract：:Recent immunohistochemical and radioimmunochemical observations have demonstrated a differential distribution of immunoreactive dynorphin (DYN) in rat brain. The presence of DYN immunoreactivity in a major intrinsic fiber pathway within the rat hippocampus (the mossy fiber system) has led us to evaluate the possible r...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(82)90210-7
更新日期：1982-10-18 00:00:00
abstract：:Rats administered cocaine 10 mg/kg i.p. exhibited hyperlocomotion which occurred within 5 min post injection. Subsequently, rats were injected with 10 mg/kg cocaine and brain and serum concentrations of cocaine were measured at 5, 10, and 20 min post injection. Within this time frame, cocaine concentration in limbic b...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(94)00339-4
更新日期：1994-01-01 00:00:00
abstract：HEADING AIMS:Abdominal aortic aneurysm (AAA) is featured by the growth impediment and apoptosis surge of VSMCs (vascular smooth muscle cells). MicroRNAs (miRNAs) are suggested to affect cellular behaviors including cell growth and apoptosis. This study concentrated on unraveling the emerging role of miR-28-5p in abdomi...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2020.117533
更新日期：2020-07-15 00:00:00
abstract：:The binding of membrane immunoglobulin (mIg) by anti-Ig antibodies is known to initiate a mitogenic signal in B lymphocytes. Because in many instances growth control appears to be correlated with phosphokinase activity, as well as with alterations in cytoskeletal architecture, we asked the question whether antibodies ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(88)90089-6
更新日期：1988-01-01 00:00:00
abstract：:Effects of sub-acute cocaine treatment on 3H-naloxone binding to 6 brain regions were examined in spontaneously hypertensive (SHR) and Wistar-Kyoto (WKY) rats. Cocaine hydrochloride (3 mg/kg, i.v.) was given by bolus injection daily for five days. Rats were decapitated 24 hr following the final injection and crude mem...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(88)90421-3
更新日期：1988-01-01 00:00:00
abstract：:Galectin-10 (gal-10, also known as Charcot-Leyden crystal protein) is a member of the galectin family of beta-galactoside binding proteins that is expressed uniquely in eosinophilic and basophilic leukocytes. To gain a better understanding of galectin gene expression, we present an analysis of the transcriptional regu...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(01)01104-3
更新日期：2001-06-01 00:00:00
abstract：:The low Km GTPase displayed an apparent Km value of 0.2-0.4 microM in P2 fractions from whole mouse brain. The activity of this enzyme ranged from 102 (pmols of GTP hydrolysed per micrograms of protein per min) in the striatum to 39 in the pons-medulla oblongata. Intermediate values were found in other structures, 74-...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(93)90176-4
更新日期：1993-01-01 00:00:00
abstract：:Retinitis pigmentosa is a very heterogeneous group of retinal degenerations, with multiple genes identified in each mode of inheritance. For autosomal dominant retinitis pigmentosa (ADRP), the most common gene is the rhodopsin (RHO) gene, mutations in which contribute to about 25% of ADRP in Caucasian population. To i...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2005.07.018
更新日期：2006-02-23 00:00:00
abstract：:To investigate the properties of the gamma-aminobutyric acid (GABA) synthesizing enzyme, glutamate decarboxylase (GAD), in the brain and the pancreatic islets of the rat, GABA concentration in the brain and the pancreatic islets was measured after administration of 3-mercaptopropionic acid (3-MP) at 25 mg/kg intraperi...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(94)00445-5
更新日期：1994-01-01 00:00:00
abstract：:It was possible to assess that ACE inhibitors bound to two different sites on the enzymes which consist in two homologous domains. Accordingly, it was also shown that testis ACE which consists in a single domain exhibits only one site for inhibitors. The common binding site of both enzymes contains Zn2+, bears the enz...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(92)90489-c
更新日期：1992-01-01 00:00:00
abstract：:Studies have reported that taraxasterol (TAR) is effective in the treatment of immune liver injury and alcoholic liver injury. The mechanism of action is mainly related to the inhibition of inflammation. To determine the key molecular mechanisms for the effect of TAR on alleviating ethanol and high-fat diet-induced li...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2020.118546
更新日期：2020-12-01 00:00:00
abstract：:The mammalian pineal gland synthesizes melatonin in a circadian manner, peaking during the dark phase. This synthesis is primarily regulated by sympathetic innervations via noradrenergic fibers, but is also modulated by many peptidergic and hormonal systems. A growing number of studies reveal a complex role for melato...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2007.10.016
更新日期：2008-01-02 00:00:00
abstract：:Rheumatoid arthritis is a chronic, inflammatory joint disease leading to inflammation of synovial membrane that lines the joints. This inflammation further progresses and results in destruction of joints and surrounding cartilages. The underlying factors can be oxidative stress, pro-inflammatory mediators, imbalance a...
journal_title：Life sciences
pub_type： 杂志文章,评审
doi：10.1016/j.lfs.2020.118459
更新日期：2020-11-15 00:00:00
abstract：:The effect of Bacillus Calmette-Guérin (BCG) treatment in allergic pulmonary reaction was studied in mice genetically selected accordingly to a High (H-IVA) or Low (L-IVA) antibody responsiveness. Mice were immunized with ovalbumin (OVA) or OVA plus BCG. Two days after nasal antigenic challenge, seric IgE and IgG1 ant...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2005.04.003
更新日期：2005-08-12 00:00:00
abstract：AIMS:microRNA-96 (miR-96) is considered as a tumor suppressor in multiple malignancies. Some studies have indicated that EZRIN could be regulated by the miR-96 in several kinds of cancer cells. However, the function of miR-96 in human osteosarcoma has not been investigated intensively so far. In this study, we mainly e...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2018.04.012
更新日期：2018-06-15 00:00:00
abstract：:Karyopherin α2 (KPNA2, also known as importinα-1), a member of the nuclear transporter family, is involved in the nucleocytoplasmic transport pathway of a variety of tumor-associated proteins. Recent studies have found that KPNA2 is overexpressed in various cancers, which is associated with poor prognosis. In addition...
journal_title：Life sciences
pub_type： 杂志文章,评审
doi：10.1016/j.lfs.2019.117140
更新日期：2020-01-15 00:00:00
abstract：:Glutathione homeostasis was investigated in unilaterally nephrectomized and sham-operated rats. Following twelve days of compensatory renal growth, it was found that the concentrations of glutathione and glutathione disulfide in representative samples of the entire remnant right kidney from the nephrectomized rats wer...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(88)90132-4
更新日期：1988-01-01 00:00:00
abstract：:Biliary excretion is the main route of disposal of bilirubin and impaired excretion results in jaundice, a well recognisable symptom of liver disease. Conjugation of bilirubin in the liver is essential for its clearance. The glucuronidation of bilirubin is catalysed by the microsomal UDP-glucuronosyltransferase UGT1A1...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2005.08.018
更新日期：2006-03-06 00:00:00
abstract：AIMS:This study investigates the effect of a new combination of glucosamine hydrochloride, chondroitin sulfate, methylsulfonylmethane, Harpagophytum procumbens root extract (standardized to 3% harpagoside) and bromelain extract (GCMHB) on formalin-induced damage to cartilage tissue in the rat knee joint and evaluates t...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2015.03.012
更新日期：2015-06-01 00:00:00
abstract：:The effect of moxisylyte hydrochloride on isolated human penile corpus cavernosum tissue was investigated and compared with other a-adrenergic antagonists. Moxisylyte produced a concentration-dependent relaxation of a norepinephrine-induced (1 x 10(-5) M) contraction of the corpus cavernosum tissue. Pretreatment with ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(89)90194-x
更新日期：1989-01-01 00:00:00
abstract：AIM:Epidermal growth factor receptor (EGFR) related reactive oxygen species (ROS) generation results in myocardial damage. We aimed to investigate the role of gefitinib (EGFR-tyrosine kinase inhibitor) in diabetic cardiomyopathy (DbCM). METHOD:DbCM was induced by injecting streptozotocin (55 mg/kg for 5 consecutive da...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2018.08.018
更新日期：2018-10-01 00:00:00
abstract：:The aim of the present study was to determine the role of adrenals in gonadal activity in the male toad during the breeding season. Exogenous administration of corticosterone or metapyrone for 6 days inhibited adrenal delta5-3beta(delta 5-3 beta) hydroxysteroid (delta5-3beta-HSD) and testicular 17beta (17 beta) hydrox...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(00)00433-1
更新日期：2000-02-18 00:00:00
abstract：AIM:Membranous nephropathy remains the most common form of the nephrotic syndrome in adults. The combination therapy of steroid and cyclophosphamide has routinely been used. Although satisfactory therapeutic efficacy can be achieved, its side effect on reproductive system has been a concern. Mizoribine is an imidazole ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2016.04.012
更新日期：2016-06-01 00:00:00
abstract：:BIM 23014 is a somatostatin analog displaying an increased biological half life due to resistance to enzymatic degradation. This peptide inhibits GH release directly at the level of pituitary somatotrophs. In addition, an action of BIM 23014 at the level of the hypothalamus is possible since somatostatinergic fibers a...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(92)90610-2
更新日期：1992-01-01 00:00:00
abstract：:The influence of the stage of the estrous cycle on binding of [3H]diazepam was examined in membranes from brains of female mice. In order to conserve endogenous factors such as progesterone, other steroids, or GABA, the assay was performed without the extensive washing procedures typically employed in measurements of ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(95)02177-k
更新日期：1995-01-01 00:00:00
