Abstract:
:Thyroid lymphocyte RNA from a Hashimoto patient with high serum levels of autoantibodies to thyroid peroxidase (TPO) was used to construct a phage display antibody library in the phagemid vector pComb3. The library (100,000cfu) encoded IgG1 heavy chains together with kappa light chains. Selection of the phages displaying TPO antibody on TPO-coated ELISA plates yielded a phage population enriched for surface expression of TPO antibody Fabs. 3 different Fabs specific for TPO were subsequently isolated with affinities in the region of 10(9) molar-1. 2 of the Fabs recognised the same, or closely related, epitopes on TPO whereas the third Fab recognised a different epitope. These 2 epitopes were recognised by TPO autoantibodies in the serum of the lymphocyte donor and a series of 10 patient sera. Available sequence data showed that several non-self antibodies and non-thyroid autoantibodies use the same V kappa and VH germline genes as TPO autoantibodies. There appeared to be no clear relationship between gene sequence or gene family usage by TPO autoantibodies of the same or similar epitope specificity.
journal_name
Autoimmunityjournal_title
Autoimmunityauthors
Hexham JM,Partridge LJ,Furmaniak J,Petersen VB,Colls JC,Pegg C,Rees Smith B,Burton DRdoi
10.3109/08916939409010651subject
Has Abstractpub_date
1994-01-01 00:00:00pages
167-79issue
3eissn
0891-6934issn
1607-842Xjournal_volume
17pub_type
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