Screening and synthesis: high throughput technologies applied to parasitology.

abstract：:Merocyanin 540 (Mc540) is a fluorescent compound which is thought to bind to membranes in which there are substantial amounts of lipid in the lipid-crystalline phase. It is shown here to be of value in detecting the transformation by both mechanical and skin-penetration methods of the cercaria to the schistosomulum. T...

journal_title：Parasitology

authors： Foley M,Kusel JR,Garland PB

更新日期：1988-02-01 00:00:00

abstract：:Cod (Gadus morhua), an important fish species in the Baltic Sea, is the paratenic host for many parasite species, including the zoonotic nematodes, Anisakis sp. and Contracaecum osculatum. We aimed to identify which invertebrate species (found in situ in the fish stomach) are responsible for infection of cod with zoon...

journal_title：Parasitology

authors： Pawlak J,Nadolna-Ałtyn K,Szostakowska B,Pachur M,Bańkowska A,Podolska M

更新日期：2019-11-01 00:00:00

abstract：:Genome sequencing has greatly contributed to our understanding of parasitic protozoa. This is particularly the case for Cryptosporidium species (phylum Apicomplexa) which are difficult to propagate. Because of their polymorphic nature, simple sequence repeats have been used extensively as genotypic markers to differen...

journal_title：Parasitology

authors： Widmer G

更新日期：2018-08-01 00:00:00

abstract：:Fourth-stage larvae of Ascaris suum, recovered from piglets at 11-22 days post-infection (p.i.), were examined by light microscopy for changes in the somatic musculature. During this time the estimated total number of muscle cells in the body increases from approximately 600 to 21 000 cells. This non-eutelic developme...

journal_title：Parasitology

authors： O'Grady RT

更新日期：1984-02-01 00:00:00

abstract：:Oesophagostomum dentatum stages were investigated for glutathione S-transferase (GST) expression at the protein and mRNA levels. GST activity was detected in all stages (infectious and parasitic stages including third- and fourth-stage larvae of different ages as well as males and females) and could be dose-dependentl...

journal_title：Parasitology

authors： Joachim A,Ruttkowski B

更新日期：2008-09-01 00:00:00

abstract：:The magnetic resonance imaging (MRI) appearance of the brain and spinal cord in humans with neuroangiostrongyliasis (NA) due to Angiostrongylus cantonensis infection has been well reported. Equivalent studies in animals are lacking. This case series describes clinical and MRI findings in 11 dogs with presumptively or ...

journal_title：Parasitology

authors： Wun MK,Malik R,Yu J,Chow KE,Lau M,Podadera JM,Webster N,Lee R,Šlapeta J,Davies S

更新日期：2020-09-21 00:00:00

abstract：:Early in the history of schistosomiasis research, children under 5 years of age were known to be infected. Although this problem was recognized over 100 years ago, insufficient action has been taken to address this issue. Under current policy, such infected children only receive their first antiparasitic treatment (pr...

journal_title：Parasitology

authors： Bustinduy AL,Wright S,Joekes EC,Kabatereine NB,Reinhard-Rupp J,King CH,Stothard JR

更新日期：2017-10-01 00:00:00

abstract：:Antibodies against the retrovirus envelope glycoprotein (gp70) of mouse xenotropic retrovirus, BALB virus 2 (Bv2) reacted with the adult worms of Schistosoma japonicum and S. mansoni. This reaction was completely inhibited after adsorption of the antibodies with virions of retrovirus. The reactive schistosome antigen ...

journal_title：Parasitology

authors： Tanaka M,Iwamura Y,Amanuma H,Irie Y,Watanabe M,Watanabe T,Uchiyama Y,Yasuraoka K

更新日期：1989-08-01 00:00:00

abstract：:In this review, we envisage the host environment, not as a hostile one, since the schistosome thrives there, but as one in which the relationship between the two organisms consists of constant communication, through signalling mechanisms involving sense organs, surface glycocalyx, surface membrane and internal organs ...

journal_title：Parasitology

authors： Kusel JR,Al-Adhami BH,Doenhoff MJ

更新日期：2007-10-01 00:00:00

abstract：:In an experiment to study the infectivity, growth and virulence of Schistocephalus solidus in their first intermediate host, copepods of the species Macrocyclops albidus were kept singly and exposed to up to 9 coracidia. Eleven or 14 days post-infection (p.i.) the presence and growth of the cestode larvae relative to ...

journal_title：Parasitology

authors： Wedekind C

更新日期：1997-09-01 00:00:00

abstract：:The amitochondriate parasitic protist Trichomonas vaginalis can utilize either glucose or maltose as carbon and energy source. The mechanisms of maltose utilization were explored with uptake experiments using radio-isotope labelled maltose in combination with the silicone-oil centrifugation technique and enzymatic ass...

journal_title：Parasitology

authors： ter Kuile BH,Müller M

更新日期：1995-01-01 00:00:00

abstract：:A 196 bp fragment of cloned kinetoplast (k) DNA of Leishmania infantum has been sequenced and shown to be diagnostic for this species. The DNA from 10(4)-10(5) promastigotes was routinely and specifically detected in crude dot blots of whole cells lysed in situ, and there was no cross-hybridization with 10(6) cells of...

journal_title：Parasitology

authors： Gramiccia M,Smith DF,Angelici MC,Ready PD,Gradoni L

更新日期：1992-08-01 00:00:00

abstract：:Ascarid parasites infect a variety of hosts and regular anthelmintic treatment is recommended for all species. Parascaris spp. is the only ascarid species with widespread anthelmintic resistance, which allows for the study of resistance mechanisms. The purpose of this study was to establish an in vitro drug exposure p...

journal_title：Parasitology

authors： Scare JA,Dini P,Norris JK,Steuer AE,Scoggin K,Gravatte HS,Howe DK,Slusarewicz P,Nielsen MK

更新日期：2020-05-01 00:00:00

abstract：:Resistance to diclazuril was induced by 10 passages of the Houghton strains of Eimeria acervulina and E. tenella in chickens given progressively greater concentrations of the drug. This resistance was, however, not complete since the drug retained some efficacy against the drug-passaged lines. Attempts to passage the ...

journal_title：Parasitology

authors： Chapman HD

更新日期：1989-10-01 00:00:00

abstract：:Toxoplasma gondii and Sarcocystis neurona are protozoan parasites with terrestrial definitive hosts, and both pathogens can cause fatal disease in a wide range of marine animals. Close monitoring of threatened southern sea otters (Enhydra lutris nereis) in California allowed for the diagnosis of dual transplacental tr...

journal_title：Parasitology

authors： Shapiro K,Miller MA,Packham AE,Aguilar B,Conrad PA,Vanwormer E,Murray MJ

更新日期：2016-03-01 00:00:00

abstract：:A delayed gamma distribution satisfactorily described the distribution of times to hatching of Ostertagia circumcincta eggs incubated in 0.1% saline at temperatures between 6 and 20 degrees C. Below 6 degree C hatching of eggs was extremely variable. The relationship between rates of development and temperature within...

journal_title：Parasitology

authors： Young RR,Anderson N,Overend D,Tweedie RL,Malafant KW,Preston GA

更新日期：1980-12-01 00:00:00

abstract：:Strongyloidiasis is frequently asymptomatic and diagnosis of latent infection is difficult due to limitations of current parasitological and serological methods. This study aimed to verify the use of conventional polymerase chain reaction (PCR) assay for molecular diagnosis of Strongyloides stercoralis infection. Fres...

journal_title：Parasitology

authors： Sitta RB,Malta FM,Pinho JR,Chieffi PP,Gryschek RC,Paula FM

更新日期：2014-04-01 00:00:00

abstract：:Leishmania are obligatory intracellular parasitic protozoa that cycle between sand fly mid-gut and phagolysosomes of mammalian macrophages. They have developed genetically programmed changes in gene and protein expression that enable rapid optimization of cell function according to vector and host environments. During...

journal_title：Parasitology

authors： Tsigankov P,Gherardini PF,Helmer-Citterich M,Zilberstein D

更新日期：2012-08-01 00:00:00

abstract：:Kudoid parasites are significant pathogens of marine fish. In the past, specific identification has been difficult due to a paucity of detailed morphological and biological information provided in the original description of some species. However, the introduction of DNA analysis has dramatically improved diagnosis. F...

journal_title：Parasitology

authors： Burger MA,Adlard RD

更新日期：2010-04-01 00:00:00

abstract：:Conventional mice and congenitally athymic, nude mice were infected with 20 metacercariae of the intestinal trematode Echinostoma revolutum. The sequential events in the pathological changes in the intestine were studied at different intervals post-infection. By day 11 onwards the conventional mice displayed dilatatio...

journal_title：Parasitology

authors： Bindseil E,Christensen NO

更新日期：1984-06-01 00:00:00

abstract：:The extent of sequence diversity among the genes encoding 10 antigens (Tp1-10) known to be recognized by CD8+ T lymphocytes from cattle immune to Theileria parva was analysed. The sequences were derived from parasites in 23 buffalo-derived cell lines, three cattle-derived isolates and one cloned cell line obtained fro...

journal_title：Parasitology

authors： Sitt T,Pelle R,Chepkwony M,Morrison WI,Toye P

更新日期：2018-09-01 00:00:00

abstract：:Many different drug-resistant lines of rodent malaria are available as screening models. It is obligatory to screen new compounds for antimalarial activity against a series of resistant lines in order to identify a compound with potential for the treatment of multi-drug resistant (MDR) malaria infections. Instead of u...

journal_title：Parasitology

authors： Tripathi R,Dhawan S,Dutta GP

更新日期：2005-09-01 00:00:00

abstract：:The complete small subunit ribosomal RNA (srRNA) gene of Theileria parva was cloned and sequenced. Two primers were designed which permitted the specific amplification of part of the Theileria srRNA gene from Theileria-infected cell line samples which were predominantly (> 95%) bovine DNA. The sequence of the central ...

journal_title：Parasitology

authors： Allsopp BA,Baylis HA,Allsopp MT,Cavalier-Smith T,Bishop RP,Carrington DM,Sohanpal B,Spooner P

更新日期：1993-08-01 00:00:00

abstract：:Harnessing the full utility of extensive gene sequences recently available for the economically important sea louse, Lepeophtheirus salmonis, requires the adaptation of modern molecular biology approaches to this non-model organism. Using a putative microsomal prostaglandin E synthase type-2 (PGES2) as a candidate gen...

journal_title：Parasitology

authors： Campbell EM,Pert CC,Bowman AS

更新日期：2009-07-01 00:00:00

abstract：:Despite the great interest to quantify the structure of host-parasite interaction networks, the real influence of some factors such as taxonomy, host body size and ecological opportunity remains poorly understood. In this paper, we investigate the general patterns of organization and structure of interactions in two a...

journal_title：Parasitology

authors： D'Bastiani E,Campião KM,Boeger WA,Araújo SBL

更新日期：2020-11-01 00:00:00

abstract：:Renal infections by parasitic ciliates were studied in adult snails of Helix aspersa aspersa and Helix aspersa maxima collected from 2 mixed rearing system-based heliciculture farms located in Galicia (NW Spain). The occurrence of ciliates was also examined in slugs (Deroceras reticulatum) invading the greenhouses whe...

journal_title：Parasitology

authors： Segade P,Kher CP,Lynn DH,Iglesias R

更新日期：2009-06-01 00:00:00

abstract：:Monoclonal antibodies reacting with pellicular antigens of Toxoplasma gondii tachyzoites have been selected among hybridomas produced against this organism by immunofluorescence assay. These antigens have been further characterized by immunofluorescence on living zoites, Western immunoblotting and immunoprecipitation ...

journal_title：Parasitology

authors： Couvreur G,Sadak A,Fortier B,Dubremetz JF

更新日期：1988-08-01 00:00:00

abstract：:Neurological involvement following trypanosome infection has been recognised for over a century. However, there are still many unanswered questions concerning the mechanisms used by the parasite to gain entry to the CNS and the pathogenesis of the resulting neuroinflammatory reaction. There is a paucity of material fr...

journal_title：Parasitology

authors： Rodgers J

更新日期：2010-12-01 00:00:00

abstract：:Diagnostic PCR primers for Trichinella were constructed. Twelve pairs of primers were designed based on the sequences of random amplified polymorphic DNA, and 4 pairs of primers were designed based on the reported sequences of complementary DNA encoding excretory-secretory glycoproteins. With these primers, 31 samples...

journal_title：Parasitology

authors： Wu Z,Nagano I,Takahashi Y

更新日期：1998-08-01 00:00:00

abstract：:The presence of introduced hosts can increase or decrease infections of co-introduced parasites in native species of conservation concern. In this study, we compared parasite abundance, intensity, and prevalence between native Awaous stamineus and introduced poeciliid fishes by a co-introduced nematode parasite (Camal...

journal_title：Parasitology

authors： Gagne RB,Heins DC,McIntyre PB,Gilliam JF,Blum MJ

更新日期：2016-10-01 00:00:00