Abstract:
:The extent of sequence diversity among the genes encoding 10 antigens (Tp1-10) known to be recognized by CD8+ T lymphocytes from cattle immune to Theileria parva was analysed. The sequences were derived from parasites in 23 buffalo-derived cell lines, three cattle-derived isolates and one cloned cell line obtained from a buffalo-derived stabilate. The results revealed substantial variation among the antigens through sequence diversity. The greatest nucleotide and amino acid diversity were observed in Tp1, Tp2 and Tp9. Tp5 and Tp7 showed the least amount of allelic diversity, and Tp5, Tp6 and Tp7 had the lowest levels of protein diversity. Tp6 was the most conserved protein; only a single non-synonymous substitution was found in all obtained sequences. The ratio of non-synonymous: synonymous substitutions varied from 0.84 (Tp1) to 0.04 (Tp6). Apart from Tp2 and Tp9, we observed no variation in the other defined CD8+ T cell epitopes (Tp4, 5, 7 and 8), indicating that epitope variation is not a universal feature of T. parva antigens. In addition to providing markers that can be used to examine the diversity in T. parva populations, the results highlight the potential for using conserved antigens to develop vaccines that provide broad protection against T. parva.
journal_name
Parasitologyjournal_title
Parasitologyauthors
Sitt T,Pelle R,Chepkwony M,Morrison WI,Toye Pdoi
10.1017/S0031182018000264subject
Has Abstractpub_date
2018-09-01 00:00:00pages
1430-1439issue
11eissn
0031-1820issn
1469-8161pii
S0031182018000264journal_volume
145pub_type
杂志文章相关文献
PARASITOLOGY文献大全abstract::The relationships between populations of the Deer Mouse (Peromyscus maniculatus) and the White-footed Mouse (P. leucopus) and their respective Cuterebra parasites were examined. Population genetic structure of hosts and parasites was inferred using cytochrome oxidase mitochondrial sequences of specimens from 7 populat...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182005007584
更新日期:2005-08-01 00:00:00
abstract::The anticoccidial effect of narasin against Eimeria flavescens, E. intestinalis, E. magna, E. perforans and E. stiedai was tested in experimentally infected rabbits. The drug was highly effective in reducing oocyst output and in preventing clinical signs. Maximum activity as measured by oocyst discharge was achieved w...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182000085309
更新日期:1981-10-01 00:00:00
abstract::An immunochromatographic test (ICT), using recombinant truncated P50 (P50t), for the detection of antibodies to Babesia gibsoni was developed and evaluated. Whereas all sera from specific pathogen-free dogs were clearly negative, all sera from dogs experimentally infected with B. gibsoni were clearly positive in the I...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182005008401
更新日期:2005-12-01 00:00:00
abstract::Neurocysticercosis (NCC) is the most common parasitic disease of the central nervous system (CNS) caused by the larval form of the tapeworm Taenia solium. NCC has a long asymptomatic period with little or no inflammation, and the sequential progression to symptomatic NCC depends upon the intense inflammation associate...
journal_title:Parasitology
pub_type: 杂志文章,评审
doi:10.1017/S0031182009991971
更新日期:2010-03-01 00:00:00
abstract::Nematodes represent one of the most abundant and species-rich groups of animals on the planet, with parasitic species causing chronic, debilitating infections in both livestock and humans worldwide. The prevalence and success of the nematodes is a direct consequence of the exceptionally protective properties of their ...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182010001113
更新日期:2011-02-01 00:00:00
abstract::We studied the component community of lung-worms of the harbour porpoise, attempting to establish the relative importance of ecological and evolutionary factors on its development. The lungs of 64 porpoises by-caught in Norwegian waters were examined for helminths. Three pseudaliid species were detected. The porpoises...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182000076186
更新日期:1994-04-01 00:00:00
abstract::Development and forward migration of Leishmania parasites in the sandfly gut is accompanied by morphological transformation to highly motile, non-dividing 'metacyclic' forms. Previous studies in vitro have demonstrated that this metacyclogenesis is associated with developmentally regulated changes in expression of two...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182000060522
更新日期:1990-12-01 00:00:00
abstract::The relative importance of temporal and spatial effects was assessed in helminth communities of bank voles (Myodes glareolus) in 3 woodland sites in N.E. Poland in the late summers of 1999 and 2002. Among common species the rank order of sites in relation to prevalence and abundance of infection was maintained between...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182008004484
更新日期:2008-07-01 00:00:00
abstract::Early in the history of schistosomiasis research, children under 5 years of age were known to be infected. Although this problem was recognized over 100 years ago, insufficient action has been taken to address this issue. Under current policy, such infected children only receive their first antiparasitic treatment (pr...
journal_title:Parasitology
pub_type: 历史文章,杂志文章,评审
doi:10.1017/S0031182017000014
更新日期:2017-10-01 00:00:00
abstract::Ticks have numerous natural enemies, but only a few species have been evaluated as tick biocontrol agents (BCAs). Some laboratory results suggest that several bacteria are pathogenic to ticks, but their mode of action and their potential value as biocontrol agents remain to be determined. The most promising entomopath...
journal_title:Parasitology
pub_type: 杂志文章,评审
doi:10.1017/s0031182004005219
更新日期:2004-01-01 00:00:00
abstract::Parasites can have a wide range of pathophysiological effects on the host. This review describes those associated with some parasites of major importance in man and animals. Haemoprotozoan diseases such as trypanosomiasis and malaria are primarily associated with anaemia. Such anaemias have a complex aetiology involvi...
journal_title:Parasitology
pub_type: 杂志文章,评审
doi:10.1017/s0031182000085814
更新日期:1987-01-01 00:00:00
abstract::During the early stages of the primary Plasmodium chabaudi chabaudi AS parasitaemia in CBA/Ca mice this parasite invaded normocytes, but as the parasitaemia developed increasing numbers of parasites were seen within reticulocytes. During and just after peak parasitaemia, as further parasite replication was controlled,...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182000058583
更新日期:1989-10-01 00:00:00
abstract::The present study was undertaken to investigate the antiparasitic activity of extracellular products of Streptomyces albus. Bioactivity-guided isolation of chloroform extracts affording a compound showing potent activity. The structure of the compound was elucidated as salinomycin (SAL) by EI-MS, 1H NMR and 13C NMR. I...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182018001919
更新日期:2019-04-01 00:00:00
abstract::Genome assemblies can form the basis of comparative analyses fostering insight into the evolutionary genetics of a parasite's pathogenicity, host-pathogen interactions, environmental constraints and invasion biology; however, the length and complexity of many parasite genomes has hampered the development of well-resol...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182017000348
更新日期:2017-09-01 00:00:00
abstract::Schizont-infected red blood cells (SI-RBC) from Plasmodium berghei-infected mice contain between 2 and 10 times as much sialic acid as uninfected RBC from the same blood (99-550 micrograms/10(10) RBC versus 33-65 micrograms/10(10) RBC). Total RBC samples from infected animals containing up to 63% ring- and trophozoite...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182000065434
更新日期:1986-06-01 00:00:00
abstract::A striking feature of toxoplasmic seroconversion is the prominent and early IgM response to a low molecular weight antigen of 4-5 kDa. Two different monoclonal antibodies directed against the 4-5 kDa antigen have been generated and used to characterize this molecule. Using these monoclonal antibodies, we could demonst...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182000068220
更新日期:1994-02-01 00:00:00
abstract::American trypanosamiasis occurs in nature as a sylvatic cycle, where Trypanosoma cruzi interacts with wild triatomines and mammalian reservoirs, such as marsupials, rodents, armadillos and other animals. Due to difficulties in trying to isolate T. cruzi stocks from the sylvatic cycle, very few studies have been perfor...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182098003709
更新日期:1999-02-01 00:00:00
abstract::The development of drug resistance by the present Houghton strain of Eimeria tenella to the quinolones, methyl benzoquate and buquinolate, was found to take place after a single experimental passage. The development of resistance was independent of drug selection pressure and showed cross resistance to other quinolone...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182000053130
更新日期:1975-08-01 00:00:00
abstract::This study quantifies the rate and intensity of re-infection with human hookworm and Schistosoma mansoni infection 12 months following successful treatment, and investigates the influence of socio-economic, geographical and environmental factors. A longitudinal study of 642 individuals aged over 5 years was conducted ...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182011001132
更新日期:2011-09-01 00:00:00
abstract::An egg-adapted line of Eimeria necatrix has now been passaged 40 times in the chorio-allantoic membranes of embryonated eggs. Between the 21st and 40th passage in eggs the parasite was subjected to a selection for precocious development, and its pre-patent period in this host is now 123-125 h. The parasite continued t...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182000044784
更新日期:1982-04-01 00:00:00
abstract::Trophically transmitted parasites must trade-off own growth on one hand and energy drain from the intermediate host on the other hand, since killing the host before transmission to the next host is a dead end for both parasites and hosts. This challenge becomes especially intriguing when multiple parasites find themse...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182016000676
更新日期:2016-09-01 00:00:00
abstract::Vertically transmitted parasites (VTPs) such as Wolbachia are expected not only to minimize the damage they inflict on their hosts, but also to protect their hosts against the damaging effects of coinfecting parasites. By modifying the fitness costs of the infection, VTPs can therefore play an important role in the ev...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182017001330
更新日期:2018-05-01 00:00:00
abstract::RNA extracted from purified parasites of Plasmodium knowlesi was fractionated using agarose gel electrophoresis. Preparations from para sites grown both in vivo and in vitro contained species of RNA with sedimentation coefficients of 4-0S, 5-0S, 16-6S, 24-2S, 31-4S, 38-0S and 48-3S. There was less RNA present in paras...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182000046655
更新日期:1975-10-01 00:00:00
abstract::The worldwide economic impact of Neospora caninum infection has caused the development of effective vaccines to become one of the main goals in the field of neosporosis research. In this study, the protection conferred by antigens from inactivated whole tachyzoites (TZ) and a tachyzoite-bradyzoite mixture (TZ-BZ) of N...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182011001156
更新日期:2011-09-01 00:00:00
abstract::The complex life-cycles of parasitic animals are a product of exploiting the process of development to generate organisms with different biological potentials within a species. Successive stages of the parasite adapt to functions such as host invasion and transmission, and to the colonization of a variety of niches, o...
journal_title:Parasitology
pub_type: 杂志文章,评审
doi:10.1017/s0031182000083451
更新日期:1989-01-01 00:00:00
abstract::Trials were carried out to study the humoral immune response of blackflies to filariae following infection using the intrathoracic injection technique. An induced 66 kDa protein was abundant in the haemolymph of the European species Simulium ornatum following infection with bovine Onchocerca lienalis. This protein was...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182000076538
更新日期:1994-12-01 00:00:00
abstract::The major transaminase in Heligmosomoides polygyrus, Panagrellus redivivus and rat liver was the 2-oxoglutarate-glutamate system, with relatively few amino acids acting as donors for the pyruvate-alanine and oxaloacetate-aspartate systems. The relative effectiveness of the different amino acid donors in the three tran...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182000081191
更新日期:1986-12-01 00:00:00
abstract:SUMMARY:The spermatozoa of ticks are anomalous in many respects: they are very large, cytoplasm-rich cells which lack a flagellum but move with a peculiar gliding motility. Their metamorphosis after deposition in the female has been well documented, but many of the subsequent events in the career of the spermatozoa are...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182009005630
更新日期:2009-04-01 00:00:00
abstract::In this study, the proteome of axenically grown Entamoeba histolytica parasites was explored by two-dimensional gel electrophoresis (2-DE), employing a practical and effective procedure for the solubilization of E. histolytica proteins. Approximately 900 protein species in the pH range between 4 and 7 were detected by...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182006001442
更新日期:2007-02-01 00:00:00
abstract::A method has been devised to show that malaria parasites can be detected serologically in infected blood with a high degree of sensitivity. Using a murine malaria model, parasites were demonstrated in a solid-phase radio-immunoassay which measured antibody-binding inhibition. Lysed red blood cells (r.b.c.) were incuba...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182000000639
更新日期:1980-02-01 00:00:00