Abstract:
:The worldwide economic impact of Neospora caninum infection has caused the development of effective vaccines to become one of the main goals in the field of neosporosis research. In this study, the protection conferred by antigens from inactivated whole tachyzoites (TZ) and a tachyzoite-bradyzoite mixture (TZ-BZ) of N. caninum (Nc-Spain7 isolate) incorporated into a water-in-oil emulsion (W/O) and aluminium hydroxide-ginseng extract (Al/G) was evaluated in mouse models of congenital and cerebral N. caninum infection. Immunization with TZ-BZ induced congenital and cerebral neosporosis exacerbation that was mainly characterized by reduced neonatal median survival time and increased parasite presence in adult mouse brains. The immune response of mice immunized with TZ-BZ was characterized by an increase in IFN-γ expression prior to challenge and an increase in IL-4 expression accompanied with significantly higher levels of antibodies against 2 recombinant bradyzoite-specific proteins (rNcSAG4 and rNcBSR4) after challenge. Immunization with TZ in W/O significantly reduced neonatal mortality, vertical transmission as well as parasite presence in adult mouse brains and induced a strong humoral immune response. The current study demonstrates the critical role of stage-specific antigens and adjuvants on the development of effective inactivated vaccines for the prevention of N. caninum infection.
journal_name
Parasitologyjournal_title
Parasitologyauthors
Rojo-Montejo S,Collantes-Fernández E,Rodríguez-Marcos S,Pérez-Zaballos F,López-Pérez I,Prenafeta A,Ortega-Mora LMdoi
10.1017/S0031182011001156subject
Has Abstractpub_date
2011-09-01 00:00:00pages
1372-83issue
11eissn
0031-1820issn
1469-8161pii
S0031182011001156journal_volume
138pub_type
杂志文章相关文献
PARASITOLOGY文献大全abstract::The extent of sequence diversity among the genes encoding 10 antigens (Tp1-10) known to be recognized by CD8+ T lymphocytes from cattle immune to Theileria parva was analysed. The sequences were derived from parasites in 23 buffalo-derived cell lines, three cattle-derived isolates and one cloned cell line obtained fro...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182018000264
更新日期:2018-09-01 00:00:00
abstract::Accurately identifying resistance to gastrointestinal nematode infections requires the ability to identify animals with low and high intensities of infection. The pathogenic effects of nematodes depend upon both the length and number of worms, neither of which can be measured in live animals. Indices that predict thes...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182014001905
更新日期:2015-05-01 00:00:00
abstract::Myxozoans have been successfully used as tags for fish stock identification around the world. However, few studies using myxozoan tags have been carried out in the Southern Atlantic, a region with complex oceanography that constitutes a potentially suitable scenario for testing the utility of myxozoans as indicators. ...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182016000226
更新日期:2016-05-01 00:00:00
abstract::Studies of the kinetics of adenosine transport were carried out on the P1 and P2 transporters of drug-sensitive Trypanosoma evansi and its cloned derivative, resistant to the melaminophenyl arsenical Mel CY. Characterization of adenosine uptake was made by estimation of the maximum concentration taken up at time infin...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s003118200300413x
更新日期:2003-12-01 00:00:00
abstract::Toxocariasis is a common helminthic infection that has a worldwide distribution. However, data from Lebanon about the prevalence of this infection are non-existent. We conducted a Toxocara seroprevalence study with 150 subjects attending the outpatient clinics at the American University of Beirut Medical Center betwee...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182005009637
更新日期:2006-05-01 00:00:00
abstract::Giardia lamblia localize and multiply in the small intestine and may cause acute or chronic diarrhoea with malabsorption of fat, protein and other nutrients. Abnormal pancreatic function has been documented in giardiasis and trophozoites directly inhibit pancreatic lipase activity in vitro. The aim of this study was t...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182000075053
更新日期:1993-04-01 00:00:00
abstract::The primary immune response to infection with Heligmosomoides polygyrus was studied in mice differing in response phenotype (fast-SWR, intermediate-NIH, slow-CBA). Marked IgG1 and IgE but not IgG2a antibody responses were detected in infected mice and the former were more intense in fast compared with slow responder s...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182000076022
更新日期:1994-05-01 00:00:00
abstract::The number of facilities in which customers make contact with cats before eating and drinking, called 'cat cafés', has recently increased in Tokyo, Japan. In a survey to clarify the possibility of zoonotic transmission in Giardia duodenalis, the infection rates of G. duodenalis in 321 stool samples of cats from 16 cat...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182010001459
更新日期:2011-04-01 00:00:00
abstract::A thymine-uracil transport system is present in the plasma membrane brush-border of the tapeworm, Hymenolepis diminuta. The relation between initial uptake and substrate concentration of either thymine or uracil was sigmoidal when 2-14C-labelled substrates were used. In contrast, absorption kinetics of methyl-14C- and...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182000052641
更新日期:1983-10-01 00:00:00
abstract::Angiostrongylus cantonensis is a metastrongyloid nematode found widely in the Asia-Pacific region, and the aetiological agent of angiostrongyliasis; a disease characterized by eosinophilic meningitis. Rattus rats are definitive hosts of A. cantonensis, while intermediate hosts include terrestrial and aquatic molluscs....
journal_title:Parasitology
pub_type: 杂志文章,评审
doi:10.1017/S0031182016000652
更新日期:2016-08-01 00:00:00
abstract::Neospora caninum is a cyst-forming parasite that causes abortion in cattle. Despite this parasite's ubiquitous distribution and wide host range, the number of N. caninum isolates obtained to date is limited. In vitro isolation of the parasite is arduous and often unsuccessful. In addition, most isolates have been obta...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S003118200800509X
更新日期:2008-12-01 00:00:00
abstract::The complete small subunit ribosomal RNA (srRNA) gene of Theileria parva was cloned and sequenced. Two primers were designed which permitted the specific amplification of part of the Theileria srRNA gene from Theileria-infected cell line samples which were predominantly (> 95%) bovine DNA. The sequence of the central ...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182000067263
更新日期:1993-08-01 00:00:00
abstract::Recent publications demonstrated that a fragment of a Neospora caninum ROP2 family member antigen represents a promising vaccine candidate. We here report on the cloning of the cDNA encoding this protein, N. caninum ROP2 family member 1 (NcROP2Fam-1), its molecular characterization and localization. The protein posses...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182013000383
更新日期:2013-07-01 00:00:00
abstract::A prediction of the hypothesis of Wilson (1977, 1980 a, b) to account for larval migration of homogonic Strongyloides ratti in the host is that the pattern of invasion of the mammary gland of a lactating rat will be quantitatively similar on both sides and independent of the point of entry into the body. Twenty-one su...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182000052744
更新日期:1982-06-01 00:00:00
abstract::Parasites of the genera Plasmodium and Haemoproteus (Apicomplexa: Haemosporida) are a diverse group of pathogens that infect birds nearly worldwide. Despite their ubiquity, the ecological and evolutionary factors that shape the diversity and distribution of these protozoan parasites among avian communities and geograp...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182017000208
更新日期:2017-06-01 00:00:00
abstract::The presence of introduced hosts can increase or decrease infections of co-introduced parasites in native species of conservation concern. In this study, we compared parasite abundance, intensity, and prevalence between native Awaous stamineus and introduced poeciliid fishes by a co-introduced nematode parasite (Camal...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182016001001
更新日期:2016-10-01 00:00:00
abstract::The major transaminase in Heligmosomoides polygyrus, Panagrellus redivivus and rat liver was the 2-oxoglutarate-glutamate system, with relatively few amino acids acting as donors for the pyruvate-alanine and oxaloacetate-aspartate systems. The relative effectiveness of the different amino acid donors in the three tran...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182000081191
更新日期:1986-12-01 00:00:00
abstract::There is increasing support for the presence of viruses and virus-like particles inside protozoan cells. This study describes viral-like RNA molecules that have been detected in two species of Eimeria that infect the chicken. The RNA molecule identified in E. maxima has been characterized: subcellular fractionation st...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182000063198
更新日期:1990-10-01 00:00:00
abstract::High throughput technologies continue to develop in response to the challenges set by the genome projects. This article discusses how the techniques of both high throughput screening (HTS) and synthesis can influence research in parasitology. Examples of the use of targeted and phenotype-based HTS using unbiased compo...
journal_title:Parasitology
pub_type: 杂志文章,评审
doi:10.1017/S0031182004007073
更新日期:2004-01-01 00:00:00
abstract::Interactions between infections of Trichinella spiralis and Nippostrongylus brasiliensis were studied in the NIH strain of mouse which is known to react strongly to T. spiralis. The course of N. brasiliensis infection in this strain of mouse is described and expulsion is shown to be accelerated in immunized mice and i...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182000000512
更新日期:1980-02-01 00:00:00
abstract::Bat flies (Streblidae) are diverse, obligate blood-feeding insects and probably the most conspicuous ectoparasites of bats. They show preferences for specific body regions on their host bat, which are reflected in behavioural characteristics. In this study, we corroborate the categorization of bat flies into three eco...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182018000318
更新日期:2018-09-01 00:00:00
abstract::The reproductive effort of Lepeophtheirus pectoralis (Müller O. F., 1776), a caligid copepod, which is commonly found infecting the European flounder, Platichthys flesus (Linnaeus, 1758), is studied in detail for the first time. Seasonal variation in body dimensions and reproductive effort are analysed. Data for 120 o...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182015001493
更新日期:2016-01-01 00:00:00
abstract::Isolates of Trypanosoma cruzi from human patients, domestic and sylvatic animals and vector insects were obtained in different areas of Argentina. Electrophoretic patterns of enzymes from extracts of 95 isolates were analysed. On the basis of zymograms providing information on 10 loci, 12 zymodemes are described accor...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182000067755
更新日期:1993-11-01 00:00:00
abstract::We investigated changes in the behaviour of Stagnicola elodes associated with the emergence of Plagiorchis elegans cercariae. Within 15 min of the reduction in light intensity, which triggered the onset of cercarial emergence, infected snails moved to the top of the water column and remained there for 2-3 h. Seventy-n...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182000077775
更新日期:1994-07-01 00:00:00
abstract::Analysis of the electrical properties of neurons in the motornervous system of Ascaris sutom suggests that it is largely an analogue system. The motorneurons do not conduct action potentials and they release transmitter tonically at their normal resting potential; transmitter release is increased or decreased as a con...
journal_title:Parasitology
pub_type: 杂志文章,评审
doi:10.1017/s0031182000077921
更新日期:1996-01-01 00:00:00
abstract::Trypanosomatids represent the causative agents of major diseases in humans, livestock and plants, with inevitable suffering and economic hardship as a result. They are also evolutionarily highly divergent organisms, and the many unique aspects of trypanosome biology provide opportunities in terms of identification of ...
journal_title:Parasitology
pub_type: 杂志文章,评审
doi:10.1017/S0031182011002125
更新日期:2012-08-01 00:00:00
abstract::Several approaches were adopted for the disruption and removal of the tegumental surface from protoscoleces of the horse strain of the hydatid organism, Echinococcus granulosus. The effectiveness of each method and the purity of subsequent microthrix-enriched fractions obtained by differential centrifugation were eval...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182000049064
更新日期:1985-02-01 00:00:00
abstract::We present a new phylogenetic analysis of 15 primate Plasmodium species based on 18S rDNA sequences including new sequences of Plasmodium coatneyi, P. fieldi, P. gonderi, P. hylobati and P. simium. The results are discussed in the context of the parasite host species and their geographical distribution. Contrary to ot...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182004006146
更新日期:2004-12-01 00:00:00
abstract::Digenean parasites infecting four Cominella whelk species (C. glandiformis, C. adspersa, C. maculosa and C. virgata), which inhabit New Zealand's intertidal zone, were analysed using molecular techniques. Mitochondrial 16S and cytochrome oxidase 1 (COI) and nuclear rDNA ITS1 sequences were used to infer phylogenetic r...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182016000494
更新日期:2016-08-01 00:00:00
abstract::The effect of sainfoin (Onobrychis viciifolia) hay consumption on the pathophysiology and local cellular responses of growing lambs during infection with Trichostrongylus colubriformis was investigated. Thirty-two lambs, 16 weeks of age, were allocated to 1 of 4 treatment groups (n=8) that were offered either grass (G...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182008004563
更新日期:2008-08-01 00:00:00