What has proteomics taught us about Leishmania development?

Abstract:

:Leishmania are obligatory intracellular parasitic protozoa that cycle between sand fly mid-gut and phagolysosomes of mammalian macrophages. They have developed genetically programmed changes in gene and protein expression that enable rapid optimization of cell function according to vector and host environments. During the last two decades, host-free systems that mimic intra-lysosomal environments have been devised in which promastigotes differentiate into amastigotes axenically. These cultures have facilitated detailed investigation of the molecular mechanisms underlying Leishmania development inside its host. Axenic promastigotes and amastigotes have been subjected to transcriptome and proteomic analyses. Development had appeared somewhat variable but was revealed by proteomics to be strictly coordinated and regulated. Here we summarize the current understanding of Leishmania promastigote to amastigote differentiation, highlighting the data generated by proteomics.

journal_name

Parasitology

journal_title

Parasitology

authors

Tsigankov P,Gherardini PF,Helmer-Citterich M,Zilberstein D

doi

10.1017/S0031182012000157

subject

Has Abstract

pub_date

2012-08-01 00:00:00

pages

1146-57

issue

9

eissn

0031-1820

issn

1469-8161

pii

S0031182012000157

journal_volume

139

pub_type

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