Antibodies against granule proteins activate neutrophils in vitro.


:Polymorphonuclear leukocyte (PMN) respiratory burst was stimulated by heterologous antibodies against PMN granule proteins but not by control antibodies. Fluorescence-activated cell sorter (FACS) analysis of activated PMN demonstrated the presence of two primary granule proteins, proteinase 3 (PR-3) and cationic protein 57 (CAP-57) at the membrane surface. The presence of myeloperoxidase (MPO) at the cell surface of primed and unprimed PMN was confirmed by immunoelectron microscopy. Priming doses of recombinant tumor necrosis alpha (rTNF alpha) enhanced the rate of superoxide (O2-) production by these antibodies and increased the amount of surface protein accessible to these antibodies. Anti-neutrophil cytoplasmic autoantibodies (ANCA) with specificities for PMN granule proteins are present in patients with Wegener's granulomatosis, polyarteritis nodosa, and idiopathic and crescentic glomerulonephritis. The demonstration that antibodies against granule proteins activate PMN supports the hypothesis that the vasculitis seen in these diseases is due in part to PMN mediated oxidative injury following PMN stimulation by ANCA.


J Leukoc Biol


Charles LA,Caldas ML,Falk RJ,Terrell RS,Jennette JC




Has Abstract


1991-12-01 00:00:00












  • Leukosialin (CD43) behavior during adhesion of human monocytic THP-1 cells to red blood cells.

    abstract::To understand the modulation and the behavior of glycocalyx elements during adhesion, we explored one of its components, the CD43 molecule, on human monocytic THP-1 cells exposed to cytokine stimulation and its redistribution during heterotypic adhesion to opsonized erythrocytes. First we demonstrated by immunofluores...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章


    authors: Soler M,Merant C,Servant C,Fraterno M,Allasia C,Lissitzky JC,Bongrand P,Foa C

    更新日期:1997-05-01 00:00:00

  • Dap12 expression in activated microglia from retinoschisin-deficient retina and its PU.1-dependent promoter regulation.

    abstract::Several alterations in the expression of immune-related transcripts were identified recently in the degenerating retina of the retinoschisin knockout (Rs1h(-/Y)) mouse, including the strong expression of the adaptor protein Dap12. As Dap12 is found in leukocytes, we hypothesized that its disease-related expression may...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章


    authors: Weigelt K,Ernst W,Walczak Y,Ebert S,Loenhardt T,Klug M,Rehli M,Weber BH,Langmann T

    更新日期:2007-12-01 00:00:00

  • Regulation and trafficking of the HLA-E molecules during monocyte-macrophage differentiation.

    abstract::HLA-E is a nonclassical HLA-class I molecule whose best known role is to protect from the natural killer cells. More recently, an additional function more similar to that of classical HLA-class I molecules, i.e., antigen presentation to T cells, is emerging. However, much remains to be explored about the intracellular...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章


    authors: Camilli G,Cassotta A,Battella S,Palmieri G,Santoni A,Paladini F,Fiorillo MT,Sorrentino R

    更新日期:2016-01-01 00:00:00

  • At the Bedside: Innate immunity as an immunotherapy tool for hematological malignancies.

    abstract::The identification of an anti-tumor effect displayed by cells of innate immunity has opened new scenarios, not only in the field of allo-HSCT but also for nontransplanted patients with hematological malignancies or solid tumors. Donor-derived NK cells have been shown to contribute to the eradication of malignant cells...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章,评审


    authors: Locatelli F,Merli P,Rutella S

    更新日期:2013-12-01 00:00:00

  • Induction of granulysin in CD8+ T cells by IL-21 and IL-15 is suppressed by human immunodeficiency virus-1.

    abstract::Immunosuppression following infection with HIV-1 predisposes patients to a myriad of opportunistic pathogens, one of the most important of which is Mtb. Granulysin, expressed by NK cells and CTL, exhibits potent antimicrobial activity against Mtb and several other opportunistic pathogens associated with HIV-1 infectio...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章


    authors: Hogg AE,Bowick GC,Herzog NK,Cloyd MW,Endsley JJ

    更新日期:2009-11-01 00:00:00

  • Colostral neutrophils express Fc alpha receptors (CD89) lacking gamma chain association and mediate noninflammatory properties of secretory IgA.

    abstract::Colostrum plays an important role in protecting newborn infants against acute gastrointestinal and respiratory infections. IgA antibodies have been considered the major effector component; however, the role of their receptors on colostral phagocytes, especially neutrophils, has not been studied. Here, we demonstrate t...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章


    authors: Honorio-França AC,Launay P,Carneiro-Sampaio MM,Monteiro RC

    更新日期:2001-02-01 00:00:00

  • Signaling networks regulating beta1 integrin-mediated adhesion of T lymphocytes to extracellular matrix.

    abstract::T-cell recognition of foreign antigen and migration to specific anatomic sites in vivo involves transient adhesive contacts between beta1 integrins expressed on T cells and cell surface proteins or extracellular-matrix components. Engagement of the CD3-T-cell receptor (CD3-TCR) complex initiates a complex signaling ca...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章,评审


    authors: Woods ML,Shimizu Y

    更新日期:2001-06-01 00:00:00

  • The polysaccharide capsule of Cryptococcus neoformans interferes with human dendritic cell maturation and activation.

    abstract::The ability of encapsulated and acapsular strains of Cryptococcus neoformans to activate dendritic cells (DC) derived from monocytes stimulated with granulocyte macrophage-colony stimulating factor and interleukin-4 was evaluated. Profound differences in DC response to encapsulated and acapsular C. neoformans strains ...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章


    authors: Vecchiarelli A,Pietrella D,Lupo P,Bistoni F,McFadden DC,Casadevall A

    更新日期:2003-09-01 00:00:00

  • Isolation and identification of feline peritoneal macrophages for in vitro studies of coronavirus-macrophage interactions.

    abstract::Feline peritoneal cells were collected by lavage with isotonic saline without the use of irritants or need for euthanasia of the cats. Macrophages were purified by centrifugation on Percoll followed by selective adherence. Although few macrophages could be obtained from an initial lavage, a second lavage performed on ...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章


    authors: Stoddart CA,Scott FW

    更新日期:1988-11-01 00:00:00

  • Interaction of murine dendritic cells with collagen up-regulates allostimulatory capacity, surface expression of heat stable antigen, and release of cytokines.

    abstract::In vivo, dendritic cells (DC) reside in direct proximity to extracellular matrix (ECM) proteins. Because ECM proteins affect morphology and function of a number of cell types, this study investigated potential effects of ECM proteins on functional properties of DC. DC were generated from murine bone marrow cultures, s...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章


    authors: Mahnke K,Bhardwaj RS,Luger TA,Schwarz T,Grabbe S

    更新日期:1996-10-01 00:00:00

  • Synthetic hexapeptides derived from the transmembrane envelope proteins of retroviruses suppress N-formylpeptide-induced monocyte polarization.

    abstract::Retroviral infections are frequently associated with immunosuppression. Retroviral transmembrane envelope proteins (TM proteins) play an important role in this phenomenon. CKS-17, a synthetic heptadecapeptide, represents the immunosuppressive site of these retroviral TM proteins. Here we support on the further delinea...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章


    authors: Oostendorp RA,Schaaper WM,Post J,Meloen RH,Scheper RJ

    更新日期:1992-03-01 00:00:00

  • Partial characterization of a mitomycin-C resistant T cell.

    abstract::A cell resistant to concentrations of mitomycin-C associated with the inactivation of many in vitro immune responses is described. This cell is responsive to concanavalin A (Con A), poorly adherent to nylon wool, present in relatively greater numbers in lymph node than other lymphoid tissues, and is relatively radiose...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章


    authors: Anderson RE,Standefer JC,Troup GM,Pogue LE

    更新日期:1987-01-01 00:00:00

  • Transcription factors that regulate monocyte/macrophage differentiation.

    abstract::Although all the cells in an organism contain the same genetic information, differences in the cell phenotype arise from the expression of lineage-specific genes. During myelopoiesis, external differentiating signals regulate the expression of a set of transcription factors. The combined action of these transcription ...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章,评审


    authors: Valledor AF,Borràs FE,Cullell-Young M,Celada A

    更新日期:1998-04-01 00:00:00

  • A single assay for measuring the rates of phagocytosis and bacterial killing by neutrophils.

    abstract::We have developed a method that enables the rates of phagocytosis and killing of bacteria by neutrophils to be measured in a single assay. Neutrophils were incubated with bacteria, and at specific intervals were separated from uningested bacteria by low speed centrifugation. Rates of phagocytosis and killing were calc...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章


    authors: Hampton MB,Vissers MC,Winterbourn CC

    更新日期:1994-02-01 00:00:00

  • Regulation by dietary essential fatty acid balance of tumor necrosis factor production in mouse macrophages.

    abstract::Increasing the dietary alpha-linolenate (18:3n - 3)/linoleate (18:2n - 6) ratio results in an increase in lipopolysaccharide (LPS)-stimulated tumor necrosis factor (TNF) production in mouse resident and casein-induced peritoneal macrophages [3]. We found that prostaglandin E2 (PGE2) production is inversely related to ...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章


    authors: Watanabe S,Onozaki K,Yamamoto S,Okuyama H

    更新日期:1993-02-01 00:00:00

  • Comparative evaluation of CC chemokine-induced migration of murine CD8alpha+ and CD8alpha- dendritic cells and their in vivo trafficking.

    abstract::Murine CD11c(+)CD8alpha(-) and CD11c(+)CD8alpha(+) dendritic cells (DCs) differentially regulate T cell responses. Although specific chemokines that recruit immature (i) or mature (m) CD8alpha(-) DCs have been identified, little is known about the influence of chemokines on CD8alpha(+) DCs. iDCs and mDCs isolated from...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章


    authors: Colvin BL,Morelli AE,Logar AJ,Lau AH,Thomson AW

    更新日期:2004-02-01 00:00:00

  • Effects of endotoxin on expression of VLA integrins by human bronchoalveolar lavage macrophages.

    abstract::Endotoxin (lipopolysaccharide, LPS) is known to induce inflammatory responses, such as monocyte/macrophage adherence, migration, and accumulation. Recruitment and accumulation of macrophages during infection and inflammation are regulated by integrin-mediated cell-extracellular matrix interactions. In the present repo...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章


    authors: Kang YH,Lee CH,Brummel SE,Newball HH,Forrester J

    更新日期:1995-04-01 00:00:00

  • Inhibiting Mer receptor tyrosine kinase suppresses STAT1, SOCS1/3, and NF-κB activation and enhances inflammatory responses in lipopolysaccharide-induced acute lung injury.

    abstract::Mer signaling participates in a novel inhibitory pathway in TLR activation. The purpose of the present study was to examine the role of Mer signaling in the down-regulation of TLR4 activation-driven immune responses in mice, i.t.-treated with LPS, using the specific Mer-blocking antibody. At 4 h and 24 h after LPS tre...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章


    authors: Lee YJ,Han JY,Byun J,Park HJ,Park EM,Chong YH,Cho MS,Kang JL

    更新日期:2012-06-01 00:00:00

  • Role of initial protein phosphorylation events and localized release-activated calcium influx in B cell antigen receptor signaling.

    abstract::An increase in intracellular calcium concentration is one of the major initial steps in B cell activation following antigen receptor (BCR) ligation. We show herein that in C57BL/6 murine B lymphocytes and in model cell lines, BCR-mediated calcium ion (Ca(2+)) influx occurs via highly selective Ca(2+) release-activated...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章


    authors: Lyubchenko T,Nielsen JP,Miller SM,Liubchenko GA,Holers VM

    更新日期:2009-02-01 00:00:00

  • MC148 encoded by human molluscum contagiosum poxvirus is an antagonist for human but not murine CCR8.

    abstract::The viral CC chemokines MC148, encoded by the poxvirus molluscum contagiosum, and viral macrophage inflammatory protein (vMIP)-I and vMIP-II, encoded by human herpesvirus 8, were probed on the murine CC receptor (CCR) 8 in parallel with human CCR8. In calcium mobilization assays, vMIP-I acted as a high-affinity agonis...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章


    authors: Lüttichau HR,Gerstoft J,Schwartz TW

    更新日期:2001-08-01 00:00:00

  • Human monocyte-mediated cytotoxicity against herpes simplex virus-infected cells: activation of cytotoxic monocytes by free and liposome-encapsulated lymphokines.

    abstract::Human peripheral blood monocytes were incubated with free or liposome-encapsulated human lymphokines containing macrophage-activating factor (MAF) and tested for their effect on herpes simplex virus (HSV)-infected target cells. Activated monocytes lysed allogeneic HSV type 2 (HSV-2)-infected whole human embryo cells a...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章


    authors: Koff WC,Showalter SD,Chakrabarty MK,Hampar B,Ceccorulli LM,Kleinerman ES

    更新日期:1985-04-01 00:00:00

  • Technical Advance: Fluorescent reporter reveals insights into eomesodermin biology in cytotoxic lymphocytes.

    abstract::The T-box transcription factor Eomes is expressed in cytotoxic immune cells and plays an important role in development, maintenance, and function of these cell types. Identification and separation of cells with differential Eomes expression would allow for better understanding of the transcriptional program governing ...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章


    authors: Paley MA,Gordon SM,Bikoff EK,Robertson EJ,Wherry EJ,Reiner SL

    更新日期:2013-02-01 00:00:00

  • Increase in the rat blood leukocyte counts induced by PAF-acether is suppressed by general anesthesia.

    abstract::Blood leukocyte count alterations induced by PAF-acether in anesthetized and nonanesthetized rats were investigated. Intravenous injection of increasing amounts of PAF-acether (1.5-8 micrograms/kg) in nonanesthetized animals induced dose-dependent hemoconcentration and leukocytosis. The former was apparent within 10 m...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章


    authors: Bozza PT,Silva PM,Castro-Faria-Neto HC,Martins MA,Cordeiro RS

    更新日期:1992-02-01 00:00:00

  • HIV replication in chronically infected macrophages is not inhibited by the Tat inhibitors Ro-5-3335 and Ro-24-7429.

    abstract::Human immunodeficiency virus infects different cell types including CD4+ lymphocytes and monocyte-derived macrophages (MDMs). We have examined the activity of the HIV-1 Tat inhibitors Ro-5-3335 and Ro-24-7429 in cultured human peripheral MDMs. Monocytes were isolated from HIV-seronegative donors by gradient centrifuga...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章


    authors: Dunne AL,Siregar H,Mills J,Crowe SM

    更新日期:1994-09-01 00:00:00

  • How neutrophils resist shear stress at blood vessel walls: molecular mechanisms, subcellular structures, and cell-cell interactions.

    abstract::Neutrophils are the first cells arriving at sites of tissue injury or infection to combat invading pathogens. Successful neutrophil recruitment to sites of inflammation highly depends on specific molecular mechanisms, fine-tuning the received information into signaling pathways and converting them into well-described ...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章,评审


    authors: Begandt D,Thome S,Sperandio M,Walzog B

    更新日期:2017-09-01 00:00:00

  • Monomeric bovine IgG2 is a potent stimulus for bovine neutrophils.

    abstract::Bovine plasma contains factors that can stimulate bovine neutrophils. Bovine plasma at 1:1,000,000-1:1,000 dilution induced a dose-dependent superoxide production in bovine neutrophils. When bovine plasma was fractionated with a protein G column, only the IgG fraction contained induction activity. At similar concentra...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章


    authors: Tao W,Corbett MJ,Pickett W

    更新日期:1995-08-01 00:00:00

  • Contrasting effects of alpha- and beta-androstenediol on oncogenic myeloid cell lines in vitro.

    abstract::The in vitro effects of 17 alpha AED, the isomer of 5-androstene-3 beta,17 beta diol (17 beta AED) on the basal growth of murine RAW 264.7, P388D1, and human HL-60 cells were investigated. 17 alpha AED treatment of RAW cells for 48 h reduced total cell number without increasing cell death as detected by trypan blue ex...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章


    authors: Huynh PN,Loria RM

    更新日期:1997-08-01 00:00:00

  • Differential screening identifies genetic markers of monocyte to macrophage maturation.

    abstract::Maturation of cells of the mononuclear phagocyte lineage from bone marrow precursors to tissue macrophages (MAC) via circulating blood monocytes (MO) is a multistep process only partially understood. Similarly, MAC differentiation can be observed if MO are cultured in vitro. In an attempt to further characterize molec...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章


    authors: Krause SW,Rehli M,Kreutz M,Schwarzfischer L,Paulauskis JD,Andreesen R

    更新日期:1996-10-01 00:00:00

  • Effect of lidocaine on production of migration inhibitory factor and on macrophage motility: in vitro exposure of guinea pig lymphocytes and macrophages.

    abstract::Exposure of lymphocytes from guinea pigs sensitized to keyhole limpet hemocyanin and of macrophages from nonsensitized animals to noncytotoxic doses of lidocaine (10(-4) to 10(-6) M) resulted in the inhibition of the production of macrophage migration inhibitory factor and of macrophage motility. The inhibition of bot...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章


    authors: Dickstein RA,Kiremidjian-Schumacher L,Stotzky G

    更新日期:1984-11-01 00:00:00

  • Signaling through Galphai2 protein is required for recruitment of neutrophils for antibody-mediated elimination of larval Strongyloides stercoralis in mice.

    abstract::The heterotrimeric guanine nucleotide-binding protein Galphai2 is involved in regulation of immune responses against microbial and nonmicrobial stimuli. Galphai2-/- mice have a selectively impaired IgM response consistent with a disorder in B cell development yet have augmented T cell effector function associated with...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章


    authors: Padigel UM,Stein L,Redding K,Lee JJ,Nolan TJ,Schad GA,Birnbaumer L,Abraham D

    更新日期:2007-04-01 00:00:00