Abstract:
BACKGROUND:Suppressive dendritic cells (DCs) are a promising tool for tolerance induction in transplantation. A human DCs subpopulation, which constitutively expresses indoleamine-2,3-dioxygenase (IDO), a molecule shown to prevent the rejection of fetus during pregnancy, has recently been described. This subset, characterized by nonadherence and CD123/CCR6 expression, exhibited sustained IDO production if exposed to interleukin (IL)-10. In the present work, we generated human nonadherent, CD123/CCR6 DCs secreting IL-10. METHODS:Monocytes were separated by plastic adherence and differentiated to DCs in the presecence of IL-3 and IL-4. Expression of IDO was determined by reverse-transcriptase polymerase chain reaction and enzyme activity by reverse-phased high-performance liquid chromatography. Mixed lymphocyte cultures were performed with allogeneic nylon wool-purified T-cells. RESULTS:Contradicting previous findings, CD123+/CCR6+ DCs did not express IDO. Maturation of these cells with inducer-cytokines up-regulated IDO, but the allogeneic T-cell stimulatory capacity of these DCs was even stronger than that of immature IDO DCs, and chemical abrogation of IDO activity did not increase T-cell proliferation. In parallel, we generated mature IDO DCs, but these cells also did not induce stronger T-cell stimulation than their IDO counterpart. CONCLUSIONS:In conclusion, CD123/CCR6 DCs do not constitutively express IDO and "induced" IDO DCs, even coexpressing anti-inflammatory IL-10, do not suppress allogeneic T-cell responses.
journal_name
Transplantationjournal_title
Transplantationauthors
Löb S,Ebner S,Wagner S,Weinreich J,Schäfer R,Königsrainer Adoi
10.1097/01.tp.0000251646.09702.ecsubject
Has Abstractpub_date
2007-02-27 00:00:00pages
468-73issue
4eissn
0041-1337issn
1534-6080pii
00007890-200702270-00015journal_volume
83pub_type
杂志文章相关文献
TRANSPLANTATION文献大全abstract:BACKGROUND:Reactivation of cytomegalovirus (CMV) is frequently observed in recipients of solid organs and bone marrow transplants and is associated with increased risk of acute and chronic allograft rejection, opportunistic infection, graft failure, and patient mortality. The molecular mechanisms by which reactivation ...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0b013e3181b242a7
更新日期:2009-09-15 00:00:00
abstract::We have studied organ allograft survival in rhesus monkeys conditioned with myeloablative total-body irradiation and T cell-depleted autologous bone marrow transplantation then given a heterotopic MHC-mismatched cardiac allograft in the immediate postmyeloablative period. This model has enabled us to investigate the r...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-198911000-00009
更新日期:1989-11-01 00:00:00
abstract:BACKGROUND:Injection of neonatal BALB/c mice with semi-allogeneic splenocytes leads to antigen-specific tolerance lasting into adulthood. Tolerant mice accept A/J skin grafts and fail to generate CD8 cytotoxic T lymphocyte (CTL) activity against A/J targets. Anergic CD8 T cells are present in tolerant mice, and CD4 reg...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199912270-00013
更新日期:1999-12-27 00:00:00
abstract:BACKGROUND:Renal ischemia-reperfusion (I/R) injury is unavoidable in kidney transplantation and frequently influences both short- and long-term allograft survival rates. One of the major events in I/R injury is the generation of cytotoxic oxygen radicals. Recently, hydrogen gas has been reported to display antioxidant ...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0b013e318255f8be
更新日期:2012-07-15 00:00:00
abstract::Subsets of T cells have been identified by the expression of different isoforms of CD45. Some T cells expressing CD45RA (gp220) have been characterized as "naive" in their response to antigens including alloantigens, whereas T cells expressing CD45RO (gp180) have been characterized as "memory" T cells. To examine the ...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199503150-00015
更新日期:1995-03-15 00:00:00
abstract::Fine-needle aspiration biopsies were used for clinical monitoring of liver allografts; 21 patients with an inflammatory episode of acute rejection (12.0 +/- 3.3 CIU at the peak) were studied; all episodes were reversible. The inflammatory infiltrate consisted mainly of lymphoid cells, including lymphoid blasts, with i...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199011000-00011
更新日期:1990-11-01 00:00:00
abstract::The frequencies of circulating donor-reactive cytotoxic lymphocyte precursors (CLP) and Il-2-producing helper lymphocyte precursors (HLP) were determined by limiting dilution analysis in 19 kidney allograft recipients before and at various intervals (up to 2 years) after transplantation. A significant, selective, and ...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199309000-00025
更新日期:1993-09-01 00:00:00
abstract::The influence of race on the outcome of cadaver renal transplantation (CRT) continues to be controversial even in the cyclosporine era. The present study examines the effect of race in 343 adult CRT performed from 1/1/82 through 10/1/88 with regard to the incidence of delayed function (DF), graft survival (GS), and pa...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199001000-00014
更新日期:1990-01-01 00:00:00
abstract:BACKGROUND:Patients at greatest risk of posttransplant lymphoproliferative disorder (PTLD) are those who acquire primary Epstein-Barr virus (EBV) infection after solid organ transplantation. The incidence of PTLD among patients who are EBV-seropositive before transplant is lower, and little is known about the differenc...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0000000000002898
更新日期:2019-11-01 00:00:00
abstract:BACKGROUND:Ischemia-reperfusion injury (IRI) significantly contributes to delayed graft function and inflammation, leading to graft loss. Ischemia-reperfusion injury is exacerbated by the thrombospondin-1-CD47 system through inhibition of nitric oxide signaling. We postulate that CD47 blockade and prevention of nitric ...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0000000000000252
更新日期:2014-08-27 00:00:00
abstract::Focal segmental glomerulosclerosis (FSGS) is the primary renal disease in approximately one-tenth of pediatric patients receiving a renal allograft. Recurrence of proteinuria after renal transplantation is observed in approximately 30% of patients and negatively impacts graft survival. Risk factors for recurrence are ...
journal_title:Transplantation
pub_type: 杂志文章,评审
doi:10.1097/01.tp.0000187110.25512.82
更新日期:2005-09-27 00:00:00
abstract::Using antisera to human beta2-microglobulin and an immunofluorescent technique, beta2-microglobulin was found to be localized along tubular and glomerular basement membranes of renal bipsies studied. Since beta2-microglobulin is a subunit of HLA preparations, it may also serve as an indirect marker for the presence of...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-197707000-00001
更新日期:1977-07-01 00:00:00
abstract::The interpretation of cellular infiltrate from renal transplant recipients with borderline (BL) changes is still a challenging problem. To analyze the immune phenotype of such infiltrate, we quantified the mRNA expression of Foxp3 and interleukinL-10 and granzyme B (GB) in 15 kidney biopsies with BL changes. Controls ...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/01.tp.0000248884.71946.19
更新日期:2007-02-15 00:00:00
abstract:BACKGROUND:T-cell receptor transgenic (TCR-tg) mouse models with direct CD4 alloreactivity will help elucidate mechanisms of transplant rejection and tolerance in vivo. Although such models exist, they are limited by unusual strain combinations or are based on model antigens. METHODS:A TCR-tg mouse with direct CD4 spe...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0b013e31815e883e
更新日期:2008-01-27 00:00:00
abstract:BACKGROUND:Successful kidney transplantation normalizes elevated proatrial natriuretic peptide (proANP) plasma concentrations of renal failure patients in the early posttransplant period. We evaluated plasma and urinary proANP fragments in the late posttransplant period. METHODS:Immunoreactive proANP(1-30) and proANP(...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-200107150-00018
更新日期:2001-07-15 00:00:00
abstract:BACKGROUND:The current shortage of organ donors has led many centers to use marginal and nonheart-beating donors (NHBDs). Recent research has implicated the infiltration of lymphocytes as an important mediator of ischemia-reperfusion injury (IRI). FTY720 is an immunosuppressant that promotes lymphocyte sequestration in...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/01.tp.0000269794.74990.da
更新日期:2007-07-27 00:00:00
abstract::We studied the role of interleukin (IL)-8 during engraftment after hematopoietic stem cell transplantation. In 40 consecutive patients undergoing either allogeneic marrow (n=32) or autologous peripheral blood stem cell transplantation (n=8), IL-8 plasma levels were serially determined. Median IL-8 concentrations peake...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/01.tp.0000135564.82707.f0
更新日期:2004-10-15 00:00:00
abstract:BACKGROUND:Combined transplantation of the lungs and liver is indicated for patients who would not be expected to survive transplantation of either organ alone. No single center has accumulated a significant experience, and as a result the expectations for this operation in the current era are unknown. METHODS:Patient...
journal_title:Transplantation
pub_type: 杂志文章,meta分析,评审
doi:10.1097/01.tp.0000165717.23652.09
更新日期:2005-11-15 00:00:00
abstract:BACKGROUND:Accurate and rapid diagnosis of human cytomegalovirus (HCMV) disease in solid organ transplant patients remains a challenge. We evaluated the clinical utility of a quantitative polymerase chain reaction (QPCR) method to diagnose transplant patients with HCMV disease. METHODS:A total of 429 plasma samples fr...
journal_title:Transplantation
pub_type: 临床试验,杂志文章
doi:10.1097/00007890-199910150-00016
更新日期:1999-10-15 00:00:00
abstract:BACKGROUND:It has been proposed that hyperacute rejection (HAR) of pig-to-primate vascularized xenografts is due in large part to ineffective regulation of recipient complement by pig complement regulatory proteins (CRPs), and indeed transgenic expression of human CRPs in pigs can prevent hyperacute rejection. However,...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-200009270-00014
更新日期:2000-09-27 00:00:00
abstract:BACKGROUND:The autonomic storm accompanying brain death leads to neurogenic pulmonary edema and triggers development of systemic and pulmonary inflammatory responses. Neurogenic vasoplegia exacerbates the pulmonary injury caused by brain death and primes the lung for ischemia reperfusion injury and primary graft dysfun...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0b013e31816398dd
更新日期:2008-02-27 00:00:00
abstract::Cyclosporine extends kidney allograft survival in the chacma baboon, and this study explores various administration protocols to generate optimal serum concentrations of the drug, assessed by radioimmunoassay and by inhibition of lymphocyte transformation by phytohemagglutinin and allogeneic lymphocytes in culture. Se...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-198308000-00001
更新日期:1983-08-01 00:00:00
abstract:BACKGROUND:There is a debate about the relative contribution of immunologic (rejection) and nonimmunologic (limited nephron mass) factors in long-term graft survival. METHODS:Using multivariate analysis, we studied the association of the following variables with outcome: delayed graft function (DGF), acute rejection, ...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-200001150-00011
更新日期:2000-01-15 00:00:00
abstract:BACKGROUND:Since suitable recipients for hepatic allografts from donors with antibodies to hepatitis B virus (HBV) have not been determined, a review of our 7-year experience with donors positive for hepatitis B surface antibody (anti-HBs), hepatitis B core antibody (anti-HBc), or both was undertaken. METHODS:Recipien...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199712150-00013
更新日期:1997-12-15 00:00:00
abstract:BACKGROUND:One of the main concerns after living donor liver transplantation is the risk of morbidity and/or mortality that it imposes on the donors. Respiratory postoperative complications in living liver donors have already been reported but their frequency seems to be underestimated. We designed a prospective study ...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/01.tp.0000191624.70135.35
更新日期:2006-01-27 00:00:00
abstract:BACKGROUND:For cyclosporine (CsA), 2-hr postdose level (C2) is the best single time point predictor of the area under the curve and a critical measure for effective dosing. The therapeutic CsA microemulsion (Neoral) C2 range in de novo heart transplant patients remains to be determined. PURPOSE:The purpose of this stu...
journal_title:Transplantation
pub_type: 杂志文章,多中心研究,随机对照试验
doi:10.1097/TP.0b013e318169bf43
更新日期:2008-04-15 00:00:00
abstract:BACKGROUND:Modification of the aminoacid sequence of peptides derived from the HLA class I heavy chain in combination with computer rational design resulted in the development of a peptide, RDP1258, with enhanced immunosuppressive activity. METHODS:We evaluated the activity of this peptide, analyzing infiltrate by imm...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199906270-00017
更新日期:1999-06-27 00:00:00
abstract::The effects of FK506 on exocrine function of the pancreas in Sprague-Dawley rats were examined and compared with those of cyclosporine. FK506 did not affect the levels of amylase and lipase in the pancreas of rats given the drug orally in a daily dose of 1 or 5 mg/kg for 2 weeks. Furthermore, the pancreas of the rats ...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199211000-00023
更新日期:1992-11-01 00:00:00
abstract::Whole-body irradiation has been extensively used to ablate immune responsiveness in rodent recipients in adoptive allograft assays. This study was undertaken to determine the relative radioresistance and the tempo of regeneration, following whole-body irradiation, of cells involved in the allograft response. Six disti...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-198809000-00018
更新日期:1988-09-01 00:00:00
abstract::Monocytes from human peripheral blood, when incubated in vitro, spread onto the surface of the glass. Horse antihuman lymphocyte globulin (ALG) added to the incubation chamber inhibits the spreading, while normal horse globulin (NHG) does not. The inhibition depends on the concentration of ALG admixed to the mononucle...
journal_title:Transplantation
pub_type: 杂志文章
doi:
更新日期:1976-01-01 00:00:00