Studies on the tissue culture of Stevia rebaudiana and its components; (II). Induction of shoot primordia.

Abstract:

:Shoot primordia, which were able to propagate vegetatively with a very high rate and to redifferentiate easily to new plants, were induced from shoot tips of Stevia rebaudiana Bertoni on Gamborg B5 medium containing 6-benzylaminopurine (BAP) and alpha-naphthaleneacetic acid (NAA) under light. The propagation of the shoot primordia of Stevia rebaudiana is rapid, and they are highly stable in chromosome number and karyotype. The shoot primordia can propagate at a high rate for a long time without differentiation. At any time, the shoot primordia readily developed into plantlets with shoots and roots within 2 or 3 weeks in static culture on B5 medium containing 0.02 mg/l BAP and 2% sucrose. The plantlets were transplanted to sterilized soil to grow to normal adult plants.

journal_name

Planta Med

journal_title

Planta medica

authors

Miyagawa H,Fujioka N,Kohda H,Yamasaki K,Taniguchi K,Tanaka R

doi

10.1055/s-2007-969165

subject

Has Abstract

pub_date

1986-08-01 00:00:00

pages

321-3

issue

4

eissn

0032-0943

issn

1439-0221

pub_type

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