Abstract：
:The expression of the neurofilament (NF) protein triplet (70, 150 and 200 k daltons (K)) was studied by immunofluorescence in two clones of murine C-1300 neuroblastoma. Clone 1 formed clumps of round cells with few processes, while in clone 2 the cells were more dispersed and extended many processes. In both clones selective decoration of tight bundles of filaments was only observed with anti-NF 70 K. Round cells contained curvilinear bundles with the appearance of whorls and ringlets , while in cells extending processes the bundles were more rectilinear in shape. Ringlets were the most prominent feature in large clumps of cells. Antisera to vimentin, the mesenchymal-type intermediate filament (IF) protein, decorated the neuroblastoma cells with a different pattern. Treatment of the two clones with dibutyryl cAMP, a neurite-inducing agent, did not result in the expression of NF 150 K and NF 200 K, although many cells had extended processes and the shape of the NF bundles had changed accordingly.
journal_name
Brain Resjournal_title
Brain researchauthors
Dahl D,Rustigian R,Bignami Adoi
10.1016/0006-8993(84)90718-2subject
Has Abstractpub_date
1984-05-14 00:00:00pages
354-7issue
2eissn
0006-8993issn
1872-6240pii
0006-8993(84)90718-2journal_volume
299pub_type
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