Abstract:
:Several regulated mRNAs were detected by applying differential display to the mouse cerebellum during postnatal development. One cDNA fragment, referred to as CPD1 (GenBank U89345), was characterized and cloned. Northern blots showed maximum mRNA expression at postnatal day seven (P7). The mRNA encodes a protein of 260 amino acids. In situ RT-PCR showed that CPD1 is expressed mainly in granule cells and faintly in Purkinje cells. Polyclonal rabbit antibodies and oligobodies (oligonucleotide-based synthetic antibodies) revealed a protein of 34 kDa in Western blots. Immunohistochemistry showed not only marked nuclear staining but also mild cytoplasmic localization. Granule cells undergoing active division (P4) showed very little expression of CPD1 protein, which increases from P7 to P17. CPD1, affinity-purified using a chemically synthesized oligobody inhibits the activity of protein phosphatase PP2A but not protein phosphatase PP1. Differentiated PC12 cells also showed nuclear and cytoplasmic localization. Interestingly, maximal cytoplasmic CPD1/PP2A colocalization was observed near cell membrane regions that are far from growing neurites, and on growing cones. These results suggest that CPD1 might have an important role in cerebellar development.
journal_name
Brain Resjournal_title
Brain researchauthors
Radrizzani M,Vilá-Ortiz G,Cafferata EG,Di Tella MC,González-Guerrico A,Perandones C,Pivetta OH,Carminatti H,Idoyaga Vargas VP,Santa-Coloma TAdoi
10.1016/s0006-8993(01)02351-4subject
Has Abstractpub_date
2001-07-13 00:00:00pages
162-74issue
1-2eissn
0006-8993issn
1872-6240pii
S0006-8993(01)02351-4journal_volume
907pub_type
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