Abstract：
:An indirect method of immunocytochemistry showed that cholera toxin and its B-subunit served as specific neuronal surface markers in conjunction with monoclonal anti-cholera toxin and FITC labeled anti-mouse Fab. The cell types which get specifically stained in culture were peripheral neurons from dorsal root ganglion cells, superior cervical ganglion cells, and cerebral neurons, all of which were rat tissue, and NGF-treated PC12 cells. Non-neuronal cells, i.e. Schwann cells, fibroblasts and glia cells, were not stained. This method can, therefore, be used to distinguish neurons from non-neuronal cells in neuronal tissue cultures, as in the case of tetanus toxin described in the literature.
journal_name
Brain Resjournal_title
Brain researchauthors
Okada E,Maeda T,Watanabe Tdoi
10.1016/0006-8993(82)90305-5subject
Has Abstractpub_date
1982-06-24 00:00:00pages
233-41issue
2eissn
0006-8993issn
1872-6240pii
0006-8993(82)90305-5journal_volume
242pub_type
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