Abstract：
:MiR-122 is one of the non-coding RNAs which showed its effects on the lipo-metablism, virus infection and HCC forming through regulation of liver gene expression. Its eukaryotic expression vector was constructed by using pSuper which was widely applied in the siRNA expression. The precursor of human miR-122 gene was amplified by polymerase chain reaction (PCR) from the human genomic DNA. The positive clones were screened by PCR and restriction enzyme digestion. The new expression vector of miR-122 was named pHsa-m122. PHsa-m122 and its controls were transfected to HepG2 cells. The miR-122 expression activity was evaluated by GFP122i sensor reporter plasmid through fluorescence detection and Western blot. It was shown that the fluorescence intensity of GFP122si and pHsa-m122 co-transfection group was weaker than that of the controls, so the functional activity of expressed miR-122 was detected. When HepG2 cells were co-transfected with HBV1.3 and pHsa-m122 plasmids, the results showed miR-122 may down-regulate the gene expression of HBV. The human liver specific microRNA eukaryotic expression vector of miR-122 was constructed successfully, which may facilitate further study of its function in the development of liver virus infection diseases and HCC.
journal_name
Cell Mol Immunoljournal_title
Cellular & molecular immunologyauthors
Chen S,Ni M,Yu B,Lv T,Lu M,Gong Fsubject
Has Abstractpub_date
2007-12-01 00:00:00pages
473-7issue
6eissn
1672-7681issn
2042-0226journal_volume
4pub_type
杂志文章abstract：:Programmed death one homolog (PD-1H) is a cell surface molecule of the B7/CD28 immune modulatory gene family. Although PD-1H has been shown to function as a coinhibitory receptor on T cells to limit naive T-cell activation and proliferation, its role in the regulation of the T-cell response to allergens is unknown. We...
journal_title：Cellular & molecular immunology
pub_type： 杂志文章
doi：10.1038/cmi.2017.16
更新日期：2018-09-01 00:00:00
abstract：:Epigenetic regulation of gene expression in cancer cells has been extensively studied in recent decades, resulting in the FDA approval of multiple epigenetic agents for treating different cancer types. Recent studies have revealed novel roles of epigenetic dysregulation in altering the phenotypes of immune cells and t...
journal_title：Cellular & molecular immunology
pub_type： 杂志文章,评审
doi：10.1038/s41423-020-0505-9
更新日期：2020-09-01 00:00:00
abstract：:Vγ2Vδ2 T (also known as Vγ9Vδ2 T) cells exist only in primates, and in humans represent a major γδ T-cell sub-population in the total population of circulating γδ T cells. Results from recent studies suggest that while (E)-4-hydroxy-3-methyl-but-2-enyl pyrophosphate (HMBPP) phosphoantigen from Mycobacterium tuberculos...
journal_title：Cellular & molecular immunology
pub_type： 杂志文章,评审
doi：10.1038/cmi.2012.46
更新日期：2013-01-01 00:00:00
abstract：:B cells are generally considered to be positive regulators of the immune response because of their capability to produce antibodies, including autoantibodies. The production of antibodies facilitates optimal CD4(+) T-cell activation because B cells serve as antigen-presenting cells and exert other modulatory functions...
journal_title：Cellular & molecular immunology
pub_type： 杂志文章,评审
doi：10.1038/cmi.2012.60
更新日期：2013-03-01 00:00:00
abstract：:Although PD-L1/PD-1 blockade therapy has been approved to treat many types of cancers, the majority of patients with solid tumors do not respond well, but the underlying reason remains unclear. Here, we studied ovarian cancer (OvCa), a tumor type generally resistant to current immunotherapies, to investigate PD-1-inde...
journal_title：Cellular & molecular immunology
pub_type： 杂志文章
doi：10.1038/s41423-019-0305-2
更新日期：2020-03-01 00:00:00
abstract：:Recent study has suggested that innate immune system might play an important role in pregnancy progression. In this study, to investigate whether NK cells and NKT cells, instead of T cells, are the dominant populations of peripheral blood in early pregnancy, flow cytometry was used to detect the percentage and intrace...
journal_title：Cellular & molecular immunology
pub_type： 杂志文章
doi：
更新日期：2007-10-01 00:00:00
abstract：:Many monoclonal antibodies (mAbs) have been extensively used in the clinic, such as rituximab to treat lymphoma. However, resistance and non-responsiveness to mAb treatment have been challenging for this line of therapy. Complement is one of the main mediators of antibody-based cancer therapy via the complement-depend...
journal_title：Cellular & molecular immunology
pub_type： 杂志文章
doi：10.1038/cmi.2010.35
更新日期：2011-03-01 00:00:00
abstract：:Macroautophagy has been implicated in modulating the therapeutic function of mesenchymal stromal cells (MSCs). However, the biological function of chaperone-mediated autophagy (CMA) in MSCs remains elusive. Here, we found that CMA was inhibited in MSCs in response to the proinflammatory cytokines interferon-γ (IFN-γ) ...
journal_title：Cellular & molecular immunology
pub_type： 杂志文章
doi：10.1038/s41423-019-0345-7
更新日期：2020-01-03 00:00:00
abstract：:Immunoglobulins (Igs) are known to be synthesized and secreted only by B lymphocytes. Class switch recombination (CSR) is a key event that enables B cells to express Igs, and one of the crucial steps for CSR initiation is the germline transcription of Ig genes. Surprisingly, recent studies have demonstrated that the I...
journal_title：Cellular & molecular immunology
pub_type： 杂志文章
doi：10.1038/cmi.2013.52
更新日期：2014-03-01 00:00:00
abstract：:A classical function of C1q is to bind immune complexes and initiate complement activation producing membrane lytic complexes, opsonins and anaphylatoxins. This classical pathway of complement activation is also elicited when C1q binds some other ligands. Besides complement activation, C1q also regulates cell differen...
journal_title：Cellular & molecular immunology
pub_type： 杂志文章,评审
doi：10.1038/cmi.2008.2
更新日期：2008-02-01 00:00:00
abstract：:CD8(+) natural killer T (NKT) cells from EBV-associated tumour patients are quantitatively and functionally impaired. EBV-induced CD8(+) NKT cells drive syngeneic T cells into a Th1-bias response to suppress EBV-associated malignancies. IL-4-biased CD4(+) NKT cells do not affect either syngeneic T cell cytotoxicity or...
journal_title：Cellular & molecular immunology
pub_type： 杂志文章
doi：10.1038/cmi.2009.48
更新日期：2009-10-01 00:00:00
abstract：:The complement system plays an important role in mediating both acquired and innate responses to defend against microbial infection, and in disposing immunoglobins and apoptotic cells. The liver (mainly hepatocytes) is responsible for biosynthesis of about 80-90% of plasma complement components and expresses a variety...
journal_title：Cellular & molecular immunology
pub_type： 杂志文章,评审
doi：
更新日期：2006-10-01 00:00:00
abstract：:We evaluated the antitumor effect of combined therapy with tegafur/uracil (UFT) plus leucovorin (LV) (UFT/LV) and protein-bound polysaccharide, PSK, in three mouse models of transplantable tumors. UFT/LV showed antitumor effect against Meth A sarcoma, and the antitumor effect was enhanced when PSK given concomitantly....
journal_title：Cellular & molecular immunology
pub_type： 杂志文章
doi：
更新日期：2007-08-01 00:00:00
abstract：:Using a newly generated monoclonal antibody (2E6) against human B7-H3, we explored the expression of the molecule on dendritic cells derived from monocytes (Mo-DCs). Its expression was examined by means of immunostaining and flow cytometric (FCM) analysis. The results showed that B7-H3 was expressed in the course of M...
journal_title：Cellular & molecular immunology
pub_type： 杂志文章
doi：
更新日期：2005-08-01 00:00:00
abstract：:In addition to serving as molecular chaperones, heat shock proteins (HSPs) have been implicated in autoimmune diseases, antigen presentation and tumor immunity. Extensive work in the last 10 years has also suggested that HSPs such as Hsp60, Hsp70, Hsp90 and gp96, may be potent activators of the innate immune system ca...
journal_title：Cellular & molecular immunology
pub_type： 杂志文章,评审
doi：
更新日期：2004-08-01 00:00:00
abstract：:Mesenchymal stem/stromal cells (MSCs) possess some characteristics of immune cells, including a pro-inflammatory phenotype, an immunosuppressive phenotype, antibacterial properties and the expression of Toll-like receptor proteins. Here we show that, similar to immune cells, MSCs retain information from danger signals...
journal_title：Cellular & molecular immunology
pub_type： 杂志文章
doi：10.1038/cmi.2015.11
更新日期：2016-05-01 00:00:00
abstract：:Immunization with inactivated autoreactive T cells (T cell vaccination) selected from individual's own T cell repertoire provides a unique in vivo setting for testing immune regulation that is known to involve interactions of a variety of related surface molecules (1). It induces regulatory immune responses that close...
journal_title：Cellular & molecular immunology
pub_type： 杂志文章,评审
doi：
更新日期：2004-10-01 00:00:00
abstract：:Kupffer cells (KCs), the liver resident macrophages accounting for 80-90% of the total population of fixed tissue macrophages in the body, not only play a key role in host defense via removing particulate materials from the portal circulation, but may also contribute to the pathogenesis of various liver diseases. We h...
journal_title：Cellular & molecular immunology
pub_type： 杂志文章
doi：10.1038/cmi.2009.56
更新日期：2009-12-01 00:00:00
abstract：:In human adipose tissue and obesity, miR-99a expression is negatively correlated with inflammation. Therefore, the present study investigated the role of miR-99a in macrophage phenotype activation and adipose tissue inflammation. M2 BMDMs showed a significant increase in miR-99a expression when compared to the M0 and ...
journal_title：Cellular & molecular immunology
pub_type： 杂志文章
doi：10.1038/s41423-018-0038-7
更新日期：2019-05-01 00:00:00
abstract：:Trogocytosis is a process which involves the transfer of membrane fragments and cell surface proteins between cells. Various types of T cells have been shown to be able to acquire membrane-bound proteins from antigen-presenting cells and their functions can be modulated following trogocytosis. However, it is not known...
journal_title：Cellular & molecular immunology
pub_type： 杂志文章
doi：10.1038/cmi.2011.62
更新日期：2012-03-01 00:00:00
abstract：:In previous research, chimerical BPI23-Fcgamma1 gene which consisted of human bactericidal/permeability increasing protein (BPI) gene of encoding the functional N terminus (amino acid residues 1 to 199) of human BPI and Fcgamma1 gene of encoding the Fc segment of human immunoglobulin G1 was successfully reconstructed ...
journal_title：Cellular & molecular immunology
pub_type： 杂志文章
doi：10.1038/cmi.2008.55
更新日期：2008-12-01 00:00:00
abstract：:Experimental autoimmune encephalomyelitis (EAE) is a good model for human multiple sclerosis (MS) research. However, there are some defects in the traditional models. Here, we improved the model by using the human myelin basic protein (MBP) as antigen. EAE was induced by immunization of female Wistar rats with human M...
journal_title：Cellular & molecular immunology
pub_type： 杂志文章
doi：
更新日期：2004-10-01 00:00:00
abstract：:Hypoxia is a major characteristic of the tumor microenvironment, and its effects on immune cells are proposed to be important factors for the process of tumor immune escape. It has been reported that hypoxia affects the function of dendritic cells and the antitumor function of T cells. Here we discuss the effects of h...
journal_title：Cellular & molecular immunology
pub_type： 杂志文章
doi：10.1038/cmi.2009.105
更新日期：2010-01-01 00:00:00
abstract：:Among various immunoregulatory molecules, the B7 family of immune-checkpoint receptors consists of highly valuable targets for cancer immunotherapy. Antibodies targeting two B7 family co-inhibitory receptors, CTLA-4 and PD-1, have elicited long-term clinical outcomes in previously refractory cancer types and are consi...
journal_title：Cellular & molecular immunology
pub_type： 杂志文章,评审
doi：10.1038/cmi.2017.148
更新日期：2018-05-01 00:00:00
abstract：:Dendritic cells are powerful antigen-presenting cells that are essential for the priming of T cell responses. In addition to providing T-cell-receptor ligands and co-stimulatory molecules for naive T cell activation and expansion, dendritic cells are thought to also provide signals for the differentiation of CD4+ T ce...
journal_title：Cellular & molecular immunology
pub_type： 杂志文章,评审
doi：10.1038/s41423-020-0465-0
更新日期：2020-06-01 00:00:00
abstract：:The capacity of pluri-potent stem cells to repair the tissues in which stem cells reside holds great promise in development of novel cell replacement therapeutics for treating chronic and degenerative diseases. However, numerous reports show that stem cell therapy, even in an autologous setting, triggers lymphocyte in...
journal_title：Cellular & molecular immunology
pub_type： 杂志文章,评审
doi：
更新日期：2007-06-01 00:00:00
abstract：:Apoptotic cell transfer has been found to be able to facilitate engraftment of allograft. However, the underlying mechanisms remain to be fully understood. Here we demonstrate that intravenous administration of donor apoptotic splenocytes can promote pancreatic islet engraftment by inducing generation of tolerogenic d...
journal_title：Cellular & molecular immunology
pub_type： 杂志文章
doi：10.1038/cmi.2013.16
更新日期：2013-09-01 00:00:00
abstract：:To elucidate the structures of SLA-DR (swine leukocyte antigen DR) genes of three Chinese pig strains (Gz, Bm and Yn), the SLA-DRA and SLA-DRB cDNA were amplified by RT-PCR and subjected to determine the sequences. The whole structures of SLA-DRA alleles are identical among three strains, consisting of 759 nucleotides...
journal_title：Cellular & molecular immunology
pub_type： 杂志文章
doi：
更新日期：2004-06-01 00:00:00
abstract：:Recognition of viral dsRNA by Toll-like receptor 3 (TLR3) leads to the induction of downstream antiviral effectors and the innate antiviral immune response. Here, we identified the zinc-finger FYVE domain-containing protein ZFYVE1, a guanylate-binding protein (GBP), as a positive regulator of TLR3-mediated signaling. ...
journal_title：Cellular & molecular immunology
pub_type： 杂志文章
doi：10.1038/s41423-019-0265-6
更新日期：2020-07-01 00:00:00
abstract：:Innate lymphoid cells (ILCs), as an important component of the innate immune system, arise from a common lymphoid progenitor and are located in mucosal barriers and various tissues, including the intestine, skin, lung, and adipose tissue. ILCs are heterogeneous subsets of lymphocytes that have emerging roles in orches...
journal_title：Cellular & molecular immunology
pub_type： 杂志文章
doi：10.1038/s41423-020-0399-6
更新日期：2020-04-01 00:00:00
