Altered beta-actin gene expression in phorbol myristate acetate-treated chondrocytes and fibroblasts.

abstract：:The int-1 proto-oncogene is a target for insertional activation of transcription by mouse mammary tumor virus in many murine mammary tumors. Whereas no expression of int-1 is seen in normal mammary tissue, int-1 RNA can be detected in normal mice in the neural tubes of midgestation embryos and in postmeiotic spermatoc...

journal_title：Molecular and cellular biology

authors： Papkoff J

更新日期：1989-08-01 00:00:00

abstract：:By immunoblotting with antibodies for phosphotyrosine, we have demonstrated that the hematopoietic growth factors interleukin-2, interleukin-3, interleukin-4, and granulocyte-macrophage colony-stimulating factor stimulate the tyrosine phosphorylation of specific sets of proteins in murine hematopoietic progenitor cell...

journal_title：Molecular and cellular biology

authors： Morla AO,Schreurs J,Miyajima A,Wang JY

更新日期：1988-05-01 00:00:00

abstract：:In this report we described the cloning and characterization of ADA5, a gene identified by resistance to GAL4-VP16-mediated toxicity. ADA5 binds directly to the VP16 activation domain but not to a transcriptionally defective VP16 double point mutant. Double mutants with mutations in ada5 and other genes (ada2 or ada3)...

journal_title：Molecular and cellular biology

authors： Marcus GA,Horiuchi J,Silverman N,Guarente L

更新日期：1996-06-01 00:00:00

abstract：:We describe a two-step strategy to alter any mouse locus repeatedly and efficiently by direct positive selection. Using conventional targeting for the first step, a functional neo gene and a nonfunctional HPRT minigene (the "socket") are introduced into the genome of HPRT- embryonic stem (ES) cells close to the chosen...

journal_title：Molecular and cellular biology

authors： Detloff PJ,Lewis J,John SW,Shehee WR,Langenbach R,Maeda N,Smithies O

更新日期：1994-10-01 00:00:00

abstract：:The TATA-binding protein (TBP), TFIIA, and TFIIB interact with promoter DNA to form a complex required for transcriptional initiation, and many transcriptional regulators function by either stimulating or inhibiting formation of this complex. We have recently identified TBP mutants that are viable in wild-type cells b...

journal_title：Molecular and cellular biology

authors： Biswas D,Imbalzano AN,Eriksson P,Yu Y,Stillman DJ

更新日期：2004-09-01 00:00:00

abstract：:A transcriptional initiator (Inr) for mammalian RNA polymerase II can be defined as a DNA sequence element that overlaps a transcription start site and is sufficient for (i) determining the start site location in a promoter that lacks a TATA box and (ii) enhancing the strength of a promoter that contains a TATA box. W...

journal_title：Molecular and cellular biology

authors： Javahery R,Khachi A,Lo K,Zenzie-Gregory B,Smale ST

更新日期：1994-01-01 00:00:00

abstract：:Human insulin-like growth factor II (IGF-II) mRNAs are subject to site-specific endonucleolytic cleavage in the 3' untranslated region, leading to an unstable 5' cleavage product containing the IGF-II coding region and a very stable 3' cleavage product of 1.8 kb. This endonucleolytic cleavage is most probably the firs...

journal_title：Molecular and cellular biology

authors： Scheper W,Meinsma D,Holthuizen PE,Sussenbach JS

更新日期：1995-01-01 00:00:00

abstract：:We previously identified a sequence-specific erythroid cell-enriched endoribonuclease (ErEN) activity involved in the turnover of the stable alpha-globin mRNA. We now demonstrate that ErEN activity is regulated by the poly(A) tail. The unadenylated alpha-globin 3' untranslated region (3'UTR) was an efficient substrate...

journal_title：Molecular and cellular biology

authors： Wang Z,Kiledjian M

更新日期：2000-09-01 00:00:00

abstract：:The transcription factor LSF, identified as a HeLa protein that binds the simian virus 40 late promoter, recognizes direct repeats with a center-to-center spacing of 10 bp. The characterization of two human cDNAs, representing alternatively spliced mRNAs, provides insight into the unusual DNA-binding and oligomerizati...

journal_title：Molecular and cellular biology

authors： Shirra MK,Zhu Q,Huang HC,Pallas D,Hansen U

更新日期：1994-08-01 00:00:00

abstract：:We used transformation to insert a selectable marker at various sites in the Saccharomyces cerevisiae genome occupied by the transposable element Ty. The vector CV9 contains the LEU2+ gene and a portion of the repeated element Ty1-17. Transformation with this plasmid resulted in integration of the vector via a recipro...

journal_title：Molecular and cellular biology

authors： Klein HL,Petes TD

更新日期：1984-02-01 00:00:00

abstract：:We have examined the expression of chimeric plasmids containing coding sequences for the herpes simplex virus thymidine kinase (tk) gene or the Tn5 gene for neomycin resistance (neo) linked to the late promoter of polyoma DNA. Although polyoma late genes are generally not expressed in transformed cells containing only...

journal_title：Molecular and cellular biology

authors： Kern FG,Basilico C

更新日期：1985-04-01 00:00:00

abstract：:The tRNA methytransferase NSun2 promotes cell proliferation, but the molecular mechanism has not been elucidated. Here, we report that NSun2 regulates cyclin-dependent kinase 1 (CDK1) expression in a cell cycle-dependent manner. Knockdown of NSun2 decreased the CDK1 protein level, while overexpression of NSun2 elevate...

journal_title：Molecular and cellular biology

authors： Xing J,Yi J,Cai X,Tang H,Liu Z,Zhang X,Martindale JL,Yang X,Jiang B,Gorospe M,Wang W

更新日期：2015-12-01 00:00:00

abstract：:Gonadotropin-releasing hormone (GnRH) interacts with a G protein-coupled receptor and increases the transcription of the glycoprotein hormone alpha-subunit gene. We have explored the possibility that mitogen-activated protein kinase (MAPK) plays a role in mediating GnRH effects on transcription. Activation of the MAPK...

journal_title：Molecular and cellular biology

authors： Roberson MS,Misra-Press A,Laurance ME,Stork PJ,Maurer RA

更新日期：1995-07-01 00:00:00

abstract：:The intracellular signaling mechanisms underlying the pathogenesis of cardiac diseases are not fully understood. We report here that selective deletion of Shp2, an SH2-containing cytoplasmic tyrosine phosphatase, in striated muscle results in severe dilated cardiomyopathy in mice, leading to heart failure and prematur...

journal_title：Molecular and cellular biology

authors： Princen F,Bard E,Sheikh F,Zhang SS,Wang J,Zago WM,Wu D,Trelles RD,Bailly-Maitre B,Kahn CR,Chen Y,Reed JC,Tong GG,Mercola M,Chen J,Feng GS

更新日期：2009-01-01 00:00:00

abstract：:The expression of the Drosophila segmentation gene fushi tarazu (ftz) is controlled at the level of transcription. The proximal enhancer, located approximately 3.4 kb upstream of the transcription start site, directs lacZ fusion gene expression in a ftz-like seven-stripe pattern in transgenic fly embryos. We have take...

journal_title：Molecular and cellular biology

authors： Han W,Yu Y,Altan N,Pick L

更新日期：1993-09-01 00:00:00

abstract：:A series of simian virus 40 (SV40)-immortalized hepatocyte cell lines were characterized for albumin production, the regulation of albumin production, and the expression of other liver-specific genes. This series of cell lines is particularly useful for studying the regulation of hepatocyte gene expression because the...

journal_title：Molecular and cellular biology

authors： Woodworth CD,Isom HC

更新日期：1987-10-01 00:00:00

abstract：:The human tumor necrosis factor alpha (TNF-alpha) gene is rapidly activated in response to multiple signals of stress and inflammation. We have identified transcription factors present in the TNF-alpha enhancer complex in vivo following ionophore stimulation (ATF-2/Jun and NFAT) and virus infection (ATF-2/Jun, NFAT, a...

journal_title：Molecular and cellular biology

authors： Falvo JV,Uglialoro AM,Brinkman BM,Merika M,Parekh BS,Tsai EY,King HC,Morielli AD,Peralta EG,Maniatis T,Thanos D,Goldfeld AE

更新日期：2000-03-01 00:00:00

abstract：:We isolated a Drosophila fickleP (ficP) mutant with a shortened copulatory duration and reduced adult-stage life span. The reduced copulatory duration is ascribable to incomplete fusion of the left and right halves of the apodeme that holds the penis during copulation. ficP is an intronic mutation occurring in the Btk...

journal_title：Molecular and cellular biology

authors： Baba K,Takeshita A,Majima K,Ueda R,Kondo S,Juni N,Yamamoto D

更新日期：1999-06-01 00:00:00

abstract：:Rad23 and Rpn10 play synergistic roles in the recognition of ubiquitinated proteins by the proteasome, and loss of both proteins causes growth and proteolytic defects. However, the physiological targets of Rad23 and Rpn10 have not been well defined. We report that rad23Delta rpn10Delta is unable to grow in the presenc...

journal_title：Molecular and cellular biology

authors： Chuang SM,Chen L,Lambertson D,Anand M,Kinzy TG,Madura K

更新日期：2005-01-01 00:00:00

abstract：:Minichromosome maintenance protein 1 (Mcm1) is required for efficient replication of autonomously replicating sequence (ARS)-containing plasmids in yeast cells. Reduced DNA binding activity in the Mcm1-1 mutant protein (P97L) results in selective initiation of a subset of replication origins and causes instability of ...

journal_title：Molecular and cellular biology

authors： Chang VK,Donato JJ,Chan CS,Tye BK

更新日期：2004-07-01 00:00:00

abstract：:When exposed to 5-azacytidine, marrow stromal cells from active long-term marrow cultures and cell lines derived from simian virus 40-transformed stromal cells rapidly upregulated c-abl and interleukin-6 transcripts while downregulating the expression of collagen I, a major matrix protein. Similar effects occurred wit...

journal_title：Molecular and cellular biology

authors： Andrews DF 3rd,Nemunaitis J,Tompkins C,Singer JW

更新日期：1989-06-01 00:00:00

abstract：:A region located at kbp -3.9 to -2.6 5' to the first hematopoietic exon of the GATA-1 gene is necessary to recapitulate gene expression in both the primitive and definitive erythroid lineages. In transfection analyses, this region activated reporter gene expression from an artificial promoter in a position- and orient...

journal_title：Molecular and cellular biology

authors： Nishimura S,Takahashi S,Kuroha T,Suwabe N,Nagasawa T,Trainor C,Yamamoto M

更新日期：2000-01-01 00:00:00

abstract：:An ACG codon, which replaces the AUG codon used to initiate the synthesis of bacteriophage T7 gene 0.3 protein, was shown to function as a low-efficiency initiation codon in a wheat germ cell-free protein-synthesizing system. ...

journal_title：Molecular and cellular biology

authors： Anderson CW,Buzash-Pollert E

更新日期：1985-12-01 00:00:00

abstract：:N-methyl-D-aspartate receptors (NMDARs) are ligand-gated ion channels that play an important role in neuronal development, plasticity, and excitotoxicity. NMDAR antagonists are neuroprotective in animal models of neuronal diseases, and the NMDAR open-channel blocker memantine is used to treat Alzheimer's disease. In v...

journal_title：Molecular and cellular biology

authors： Kahlfuß S,Simma N,Mankiewicz J,Bose T,Lowinus T,Klein-Hessling S,Sprengel R,Schraven B,Heine M,Bommhardt U

更新日期：2014-03-01 00:00:00

abstract：:An enzyme was isolated from a eucaryotic, Chlorella-like green alga infected with the virus PBCV-1 which exhibits type II restriction endonuclease activity. The enzyme recognized the sequence GATC and cleaved DNA 5' to the G. Methylation of deoxyadenosine in the GATC sequence inhibited enzyme activity. In vitro the en...

journal_title：Molecular and cellular biology

authors： Xia YN,Burbank DE,Uher L,Rabussay D,Van Etten JL

更新日期：1986-05-01 00:00:00

abstract：:Recent research has identified critical roles for microRNAs in a large number of cellular processes, including tumorigenic transformation. While significant progress has been made towards understanding the mechanisms of gene regulation by microRNAs, much less is known about factors affecting the expression of these no...

journal_title：Molecular and cellular biology

authors： Kulshreshtha R,Ferracin M,Wojcik SE,Garzon R,Alder H,Agosto-Perez FJ,Davuluri R,Liu CG,Croce CM,Negrini M,Calin GA,Ivan M

更新日期：2007-03-01 00:00:00

abstract：:SEC62 is required for the import of secretory protein precursors into the endoplasmic reticulum (ER) of Saccharomyces cerevisiae. The DNA sequence of SEC62 predicts a 32-kDa polypeptide with two potential membrane-spanning segments. Two antisera directed against different portions of the SEC62 coding region specifical...

journal_title：Molecular and cellular biology

authors： Deshaies RJ,Schekman R

更新日期：1990-11-01 00:00:00

abstract：:We utilized the mouse mammary tumor virus (MMTV) long terminal repeat (LTR) in vivo to understand how the interaction of the glucocorticoid receptor (GR) with a nucleosome-assembled promoter allows access of factors required for the transition from a repressed promoter to a derepressed, transcriptionally competent pro...

journal_title：Molecular and cellular biology

authors： Sheldon LA,Smith CL,Bodwell JE,Munck AU,Hager GL

更新日期：1999-12-01 00:00:00

abstract：:The complete nucleotide sequence of a genomic clone encoding the mouse skeletal alpha-actin gene has been determined. This single-copy gene codes for a protein identical in primary sequence to the rabbit skeletal alpha-actin. It has a large intron in the 5'-untranslated region 12 nucleotides upstream from the initiato...

journal_title：Molecular and cellular biology

authors： Hu MC,Sharp SB,Davidson N

更新日期：1986-01-01 00:00:00

abstract：:In Caenorhabditis elegans, the central cell-killing process is essentially controlled by the interplay of four apoptotic factors: EGL-1/BH3-only protein, CED-9/Bcl2, CED-4/Apaf1, and CED-3/caspase. In cells destined to die, EGL-1 binds to CED-9 and results in the release of CED-4 from the mitochondrion-tethered CED-9-...

journal_title：Molecular and cellular biology

authors： Shen Q,Qin F,Gao Z,Cui J,Xiao H,Xu Z,Yang C

更新日期：2009-07-01 00:00:00