Abstract:
:We isolated a Drosophila fickleP (ficP) mutant with a shortened copulatory duration and reduced adult-stage life span. The reduced copulatory duration is ascribable to incomplete fusion of the left and right halves of the apodeme that holds the penis during copulation. ficP is an intronic mutation occurring in the Btk gene, a gene which encodes two forms (type 1 and type 2) of a Bruton's tyrosine kinase (Btk) family cytoplasmic tyrosine kinase as a result of alternative exon usage. The ficP mutation prevents the formation of the type 2 isoform but leaves expression of the type 1 transcript intact. Ubiquitous overexpression of the wild-type cDNA by using a heat shock 70 promoter during the late larval or pupal stages rescued the life span and genital defects in the mutant, respectively, establishing the causal relationship between the ficP phenotypes and the Btk gene mutation. The stage specificity of the rescuing ability suggests that the Btk gene is required for the development of male genitalia and substrates required for adult survival.
journal_name
Mol Cell Bioljournal_title
Molecular and cellular biologyauthors
Baba K,Takeshita A,Majima K,Ueda R,Kondo S,Juni N,Yamamoto Ddoi
10.1128/mcb.19.6.4405subject
Has Abstractpub_date
1999-06-01 00:00:00pages
4405-13issue
6eissn
0270-7306issn
1098-5549journal_volume
19pub_type
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