Abstract:
:A cDNA library enriched with Myc-responsive cDNAs but depleted of myc cDNAs was used in a functional screen for growth enhancement in c-myc-null cells. A cDNA clone for mitochondrial serine hydroxymethyltransferase (mSHMT) that was capable of partial complementation of the growth defects of c-myc-null cells was identified. Expression analysis and chromatin immunoprecipitation demonstrated that mSHMT is a direct Myc target gene. Furthermore, a separate gene encoding the cytoplasmic isoform of the same enzyme is also a direct target of Myc regulation. SHMT enzymes are the major source of the one-carbon unit required for folate metabolism and for the biosynthesis of nucleotides and amino acids. Our data establish a novel functional link between Myc and the regulation of cellular metabolism.
journal_name
Mol Cell Bioljournal_title
Molecular and cellular biologyauthors
Nikiforov MA,Chandriani S,O'Connell B,Petrenko O,Kotenko I,Beavis A,Sedivy JM,Cole MDdoi
10.1128/mcb.22.16.5793-5800.2002subject
Has Abstractpub_date
2002-08-01 00:00:00pages
5793-800issue
16eissn
0270-7306issn
1098-5549journal_volume
22pub_type
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