RAD18 and poly(ADP-ribose) polymerase independently suppress the access of nonhomologous end joining to double-strand breaks and facilitate homologous recombination-mediated repair.

abstract：:DNA from the centromere region of linkage group (LG) VII of Neurospora crassa was cloned previously from a yeast artificial chromosome library and was found to be atypical of Neurospora DNA in both composition (AT rich) and complexity (repetitive). We have determined the DNA sequence of a small portion (approximately ...

journal_title：Molecular and cellular biology

authors： Cambareri EB,Aisner R,Carbon J

更新日期：1998-09-01 00:00:00

abstract：:DHCR24/seladin-1, a crucial enzyme in sterol synthesis, is of lower abundance in brain areas affected by Alzheimer's disease. While high levels of DHCR24/seladin-1 exert antiapoptotic function by conferring resistance against oxidative stress, the molecular mechanism for this protective effect is not fully understood....

journal_title：Molecular and cellular biology

authors： Kuehnle K,Crameri A,Kälin RE,Luciani P,Benvenuti S,Peri A,Ratti F,Rodolfo M,Kulic L,Heppner FL,Nitsch RM,Mohajeri MH

更新日期：2008-01-01 00:00:00

abstract：:The Drosophila extra sex combs (esc) protein, a member of the Polycomb group (PcG), is a transcriptional repressor of homeotic genes. Genetic studies have shown that esc protein is required in early embryos at about the time that other PcG proteins become engaged in homeotic gene repression. The esc protein consists p...

journal_title：Molecular and cellular biology

authors： Ng J,Li R,Morgan K,Simon J

更新日期：1997-11-01 00:00:00

abstract：:Testis-brain RNA-binding protein (TB-RBP), the mouse orthologue of the human protein Translin, is a widely expressed and highly conserved protein with proposed functions in chromosomal translocations, mitotic cell division, and mRNA transport and storage. To better define the biological roles of TB-RBP, we generated m...

journal_title：Molecular and cellular biology

authors： Chennathukuzhi V,Stein JM,Abel T,Donlon S,Yang S,Miller JP,Allman DM,Simmons RA,Hecht NB

更新日期：2003-09-01 00:00:00

abstract：:mTOR coordinates growth signals with metabolic pathways and protein synthesis and is hyperactivated in many human cancers. mTOR exists in two complexes: mTORC1, which stimulates protein, lipid, and ribosome biosynthesis, and mTORC2, which regulates cytoskeleton functions. While mTOR is known to be involved in the DNA ...

journal_title：Molecular and cellular biology

authors： Silvera D,Ernlund A,Arju R,Connolly E,Volta V,Wang J,Schneider RJ

更新日期：2017-02-15 00:00:00

abstract：:Genomic DNA in higher eucaryotic cells is organized into a series of loops, each of which may be affixed at its base to the nuclear matrix via a specific matrix attachment region (MAR). In this report, we describe the distribution of MARs within the amplified dihydrofolate reductase (DHFR) domain (amplicon) in the met...

journal_title：Molecular and cellular biology

authors： Dijkwel PA,Hamlin JL

更新日期：1988-12-01 00:00:00

abstract：:The dynamic SCF (Skp1-cullin1-F-box protein) assembly is controlled by cycles of cullin neddylation/deneddylation based on the deneddylation activity of the COP9 signalosome (CSN) and global sequestration of cullins by CAND1. However, acceptance of this prediction was hampered by the results of recent studies, and the...

journal_title：Molecular and cellular biology

authors： Liu Q,Zhou Y,Tang R,Wang X,Hu Q,Wang Y,He Q

更新日期：2017-11-13 00:00:00

abstract：:Glucose release from hepatocytes is important for maintenance of blood glucose levels. Glucose-6-phosphate phosphatase, catalyzing the final metabolic step of gluconeogenesis, faces the endoplasmic reticulum (ER) lumen. Thus, glucose produced in the ER has to be either exported from the ER into the cytosol before rele...

journal_title：Molecular and cellular biology

authors： Fehr M,Takanaga H,Ehrhardt DW,Frommer WB

更新日期：2005-12-01 00:00:00

abstract：:Although TRAIL is considered a potential anticancer agent, it enhances tumor progression by activating NF-κB in apoptosis-resistant cells. Cellular FLICE-like inhibitory protein (cFLIP) overexpression and caspase-8 activation have been implicated in TRAIL-induced NF-κB activation; however, the underlying mechanisms ar...

journal_title：Molecular and cellular biology

authors： Zhang L,Blackwell K,Workman LM,Chen S,Pope MR,Janz S,Habelhah H

更新日期：2015-10-01 00:00:00

abstract：:The protein-tyrosine kinases (PTKs) are a burgeoning family of proteins, each of which bears a conserved domain of 250 to 300 amino acids capable of phosphorylating substrate proteins on tyrosine residues. We recently exploited the existence of two highly conserved sequence elements within the catalytic domain to gene...

journal_title：Molecular and cellular biology

authors： Wilks AF,Harpur AG,Kurban RR,Ralph SJ,Zürcher G,Ziemiecki A

更新日期：1991-04-01 00:00:00

abstract：:Coagulation factor VIII is an essential cofactor required for normal hemostatic function. A deficiency in factor VIII results in the bleeding disorder hemophilia A. Despite the fact that the factor VIII gene was cloned a decade ago, the mechanisms which control its transcription remain unresolved. In our studies, we h...

journal_title：Molecular and cellular biology

authors： McGlynn LK,Mueller CR,Begbie M,Notley CR,Lillicrap D

更新日期：1996-05-01 00:00:00

abstract：:Autoimmune diseases are characterized by spontaneously occurring autoantibodies which have proven to be useful reagents for the characterization of specific nuclear proteins. Using a monoclonal autoantibody (72B9) derived from a murine lupus strain, we have cloned a cDNA from the human T-cell line MOLT-4, which encode...

journal_title：Molecular and cellular biology

authors： Pollard KM,Chan EK,Grant BJ,Sullivan KF,Tan EM,Glass CA

更新日期：1990-05-01 00:00:00

abstract：:By using recombinase-mediated cassette exchange, a method that allows integration of single copies of different constructs at the same predetermined chromosomal location, several expression cassettes have been integrated at a randomly chosen locus in the genome of mouse erythroleukemia cells. The cassettes studied con...

journal_title：Molecular and cellular biology

authors： Feng YQ,Alami R,Bouhassira EE

更新日期：1999-07-01 00:00:00

abstract：:Using a Saccharomyces cerevisiae strain containing an integrated copy of an H2A-lacZ fusion gene, we screened for mutants which overexpressed beta-galactosidase as a way to identify genes which regulate transcription of the histone genes. Five recessive mutants with this phenotype were shown to contain altered regulat...

journal_title：Molecular and cellular biology

authors： Osley MA,Lycan D

更新日期：1987-12-01 00:00:00

abstract：:We have previously formulated a list of approximately 2,000 RNA octamers as putative exonic splicing enhancers (PESEs) based on a statistical comparison of human exonic and nonexonic sequences (X. H. Zhang and L. A. Chasin, Genes Dev. 18:1241-1250, 2004). When inserted into a poorly spliced test exon, all eight tested...

journal_title：Molecular and cellular biology

authors： Zhang XH,Kangsamaksin T,Chao MS,Banerjee JK,Chasin LA

更新日期：2005-08-01 00:00:00

abstract：:Activation of transcription can occur by the facilitated recruitment of TFIID to promoters by gene-specific activators. To investigate the role of TFIIA in TFIID recruitment in vivo, we exploited a class of yeast TATA-binding protein (TBP) mutants that is activation and DNA binding defective. We found that co-overexpr...

journal_title：Molecular and cellular biology

authors： Liu Q,Gabriel SE,Roinick KL,Ward RD,Arndt KM

更新日期：1999-12-01 00:00:00

abstract：:The major cytoskeletal actin gene of Drosophila melanogaster, the actin 5C gene, has two promoters, the distal one of which controls synthesis of actin in a tissue- and developmental stage-specific manner. This very strong promoter has widely been used for expression of heterologous genes in cultured cells. To locate ...

journal_title：Molecular and cellular biology

authors： Chung YT,Keller EB

更新日期：1990-12-01 00:00:00

abstract：:Individuals with the inherited cancer predisposition syndrome neurofibromatosis 2 (NF2) develop several central nervous system (CNS) malignancies, including glial cell neoplasms (ependymomas). Recent studies have suggested that the NF2 protein, merlin (or schwannomin), may regulate receptor tyrosine kinase signaling, ...

journal_title：Molecular and cellular biology

authors： Houshmandi SS,Emnett RJ,Giovannini M,Gutmann DH

更新日期：2009-03-01 00:00:00

abstract：:Using a subtractive cloning scheme on cDNA prepared from primary pro-B and pre-B cells, we identified several genes whose products regulate apoptosis. We further characterized one of these genes, encoding protein kinase Ceta (PKCeta). PKCeta transcripts were readily detected in pro-B cells but were absent in pre-B cel...

journal_title：Molecular and cellular biology

authors： Morrow TA,Muljo SA,Zhang J,Hardwick JM,Schlissel MS

更新日期：1999-08-01 00:00:00

abstract：:The 5'-flanking DNA sequences involved in the specific and efficient transcription of the polyoma virus early region have been investigated. Sequence requirements for efficient in vivo expression differed from those in vitro. Deletion of DNA located between 200 and 400 base pairs before the principal cap sites severel...

journal_title：Molecular and cellular biology

authors： Jat P,Novak U,Cowie A,Tyndall C,Kamen R

更新日期：1982-07-01 00:00:00

abstract：:Copper is an essential cofactor of two mitochondrial enzymes: cytochrome c oxidase (COX) and Cu-Zn superoxide dismutase (Sod1p). Copper incorporation into these enzymes is facilitated by metallochaperone proteins which probably use copper from a mitochondrial matrix-localized pool. Here we describe a novel conserved m...

journal_title：Molecular and cellular biology

authors： Horn D,Al-Ali H,Barrientos A

更新日期：2008-07-01 00:00:00

abstract：:Posttranslational modifications (PTMs) can alter many fundamental properties of a protein. One or combinations of them have been known to regulate the dynamics of many cellular pathways and consequently regulate all vital processes. Understandably, pathogens have evolved sophisticated strategies to subvert these mecha...

journal_title：Molecular and cellular biology

authors： Verma S,Mohapatra G,Ahmad SM,Rana S,Jain S,Khalsa JK,Srikanth CV

更新日期：2015-09-01 00:00:00

abstract：:DNA in the polyploid macronucleus of the ciliated protozoan Tetrahymena thermophila contains the modified base N6-methyladenine. We identified two GATC sites which are methylated in most or all of the 45 copies of the macronuclear genome. One site is 2 kilobases 5' to the histone H4-I gene, and the other is 5 kilobase...

journal_title：Molecular and cellular biology

authors： Harrison GS,Findly RC,Karrer KM

更新日期：1986-07-01 00:00:00

abstract：:The RPC31 gene encoding the C31 subunit of Saccharomyces cerevisiae RNA polymerase C (III) has been isolated, starting from a C-terminal fragment cloned on a lambda gt11 library. It is unique on the yeast genome and lies on the left arm of chromosome XIV, very close to a NotI site. Its coding sequence perfectly matche...

journal_title：Molecular and cellular biology

authors： Mosrin C,Riva M,Beltrame M,Cassar E,Sentenac A,Thuriaux P

更新日期：1990-09-01 00:00:00

abstract：:The class II transactivator (CIITA) is induced by gamma interferon (IFN-gamma) and activates major histocompatibility complex class II; however, this report shows it suppresses other genes. An N-terminal 36 amino acids of CIITA mediates suppression of the collagen alpha(2)(I) promoter via binding to CREB-binding prote...

journal_title：Molecular and cellular biology

authors： Zhu XS,Ting JP

更新日期：2001-10-01 00:00:00

abstract：:The Wnt signaling pathway plays a pivotal role in vertebrate early development and morphogenesis. Duplin (axis duplication inhibitor) interacts with beta-catenin and prevents its binding to Tcf, thereby inhibiting downstream Wnt signaling. Here we show that Duplin is expressed predominantly from early- to mid-stage mo...

journal_title：Molecular and cellular biology

authors： Nishiyama M,Nakayama K,Tsunematsu R,Tsukiyama T,Kikuchi A,Nakayama KI

更新日期：2004-10-01 00:00:00

abstract：:Little is known about the mechanisms that regulate differential transactivation by p53. We developed a system in the yeast Saccharomyces cerevisiae that addresses p53 transactivation capacity from 26 different p53 response elements (REs) under conditions where all other factors, such as chromatin, are kept constant. T...

journal_title：Molecular and cellular biology

authors： Inga A,Storici F,Darden TA,Resnick MA

更新日期：2002-12-01 00:00:00

abstract：:Eukaryotic ribosome biogenesis requires the action of approximately 200 trans-acting factors and the incorporation of 79 ribosomal proteins (RPs). The delivery of RPs to preribosomes is a major challenge for the cell because RPs are often highly basic and contain intrinsically disordered regions prone to nonspecific i...

journal_title：Molecular and cellular biology

authors： Black JJ,Musalgaonkar S,Johnson AW

更新日期：2019-08-12 00:00:00

abstract：:Insulin treatment of Chinese hamster ovary (CHO) cells expressing high levels of the insulin receptor (CHO/IR cells) activates both c-fos serum response element and activator protein 1 (AP-1) reporter genes approximately 10-fold. In contrast, parental CHO cells display only two- to threefold insulin stimulation of rep...

journal_title：Molecular and cellular biology

authors： Yamauchi K,Pessin JE

更新日期：1994-07-01 00:00:00

abstract：:An extracellular acid phosphatase was detected in the growth media of Leishmania donovani promastigotes. The enzyme was released at all stages of the growth cycle and in amounts which accounted for 90% of the total amount of this enzyme in the culture. The exoenzyme exhibited a pH optimum of 4.5 to 5.0 and was active ...

journal_title：Molecular and cellular biology

authors： Gottlieb M,Dwyer DM

更新日期：1982-01-01 00:00:00