Analysis of TFIIA function In vivo: evidence for a role in TATA-binding protein recruitment and gene-specific activation.

abstract：:Retrovirus vector infection was used to introduce large numbers of unique genetic markers into tumor cell populations for the purpose of analyzing comparative changes in the clonal composition of metastatic versus that of nonmetastatic tumors during their progressive growth in vivo. The cell lines used were SP1, a non...

journal_title：Molecular and cellular biology

authors： Korczak B,Robson IB,Lamarche C,Bernstein A,Kerbel RS

更新日期：1988-08-01 00:00:00

abstract：:Using competition mobility shift, methylation interference, and proteolytic clipping DNA binding assays, we demonstrate that the protein binding the major histocompatibility complex A beta CCAAT box is indistinguishable from the protein previously named NF-Y, which binds the major histocompatibility complex E alpha CC...

journal_title：Molecular and cellular biology

authors： Kern MJ,Woodward JG

更新日期：1991-01-01 00:00:00

abstract：:When exposed to 5-azacytidine, marrow stromal cells from active long-term marrow cultures and cell lines derived from simian virus 40-transformed stromal cells rapidly upregulated c-abl and interleukin-6 transcripts while downregulating the expression of collagen I, a major matrix protein. Similar effects occurred wit...

journal_title：Molecular and cellular biology

authors： Andrews DF 3rd,Nemunaitis J,Tompkins C,Singer JW

更新日期：1989-06-01 00:00:00

abstract：:Nucleoporins mediate transport of macromolecules across the nuclear pore complex, yet the function of many individual nucleoporins is largely unresolved. To address this question, we depleted cells of the cytoplasmic nucleoporins Nup214/CAN and Nup358/RanBP2 by RNA interference. Depletion of Nup214 resulted in codeple...

journal_title：Molecular and cellular biology

authors： Hutten S,Kehlenbach RH

更新日期：2006-09-01 00:00:00

abstract：:Eukaryotic elongation factor 1 alpha 2 (eEF1A2) is a transforming gene product that is highly expressed in human tumors of the ovary, lung, and breast. eEF1A2 also stimulates actin remodeling, and the expression of this factor is sufficient to induce the formation of filopodia, long cellular processes composed of bund...

journal_title：Molecular and cellular biology

authors： Jeganathan S,Morrow A,Amiri A,Lee JM

更新日期：2008-07-01 00:00:00

abstract：:RSC is an essential, multisubunit chromatin remodeling complex. We show here that the Rsc4 subunit of RSC interacted via its C terminus with Rpb5, a conserved subunit shared by all three nuclear RNA polymerases (Pol). Furthermore, the RSC complex coimmunoprecipitated with all three RNA polymerases. Mutations in the C ...

journal_title：Molecular and cellular biology

authors： Soutourina J,Bordas-Le Floch V,Gendrel G,Flores A,Ducrot C,Dumay-Odelot H,Soularue P,Navarro F,Cairns BR,Lefebvre O,Werner M

更新日期：2006-07-01 00:00:00

abstract：:Wound healing in certain individuals leads to the development of keloid tumors which exhibit abnormal collagen metabolism and an increased abundance of extracellular matrix components. Comparison of fibronectin levels in fibroblasts derived from keloids and normal dermis revealed a relative increase in intracellular a...

journal_title：Molecular and cellular biology

authors： Babu M,Diegelmann R,Oliver N

更新日期：1989-04-01 00:00:00

abstract：:IMP dehydrogenase (IMPDH) is the rate-limiting enzyme in the de novo synthesis of guanine nucleotides. It is a target of therapeutically useful drugs and is implicated in the regulation of cell growth rate. In the yeast Saccharomyces cerevisiae, mutations in components of the RNA polymerase II (Pol II) transcription e...

journal_title：Molecular and cellular biology

authors： Shaw RJ,Reines D

更新日期：2000-10-01 00:00:00

abstract：:We describe the cloning and characterization of tumor necrosis factor receptor (TNF-R)-associated ubiquitous scaffolding and signaling protein (TRUSS), a novel TNF-R1-interacting protein of 90.7 kDa. TRUSS mRNA was ubiquitously expressed in mouse tissues but was enriched in heart, liver, and testis. Co-immunoprecipita...

journal_title：Molecular and cellular biology

authors： Soond SM,Terry JL,Colbert JD,Riches DW

更新日期：2003-11-01 00:00:00

abstract：:We have examined the possible role of adenosine 3',5'-phosphate (cAMP) in functions associated with the plasma membranes of Saccharomyces cerevisiae. Purified membranes from this source contained an adenylate cyclase which was insensitive to activation by fluoride or guanine nucleotides, only weakly responsive to chan...

journal_title：Molecular and cellular biology

authors： Jaynes PK,McDonough JP,Mahler HR

更新日期：1982-12-01 00:00:00

abstract：:There are four members of the myocyte enhancer factor 2 (MEF2) family of transcription factors in vertebrates, MEF2A, -B, -C, and -D, which have homology within a MADS box at their amino termini and an adjacent motif known as the MEF2 domain. These factors activate muscle gene expression by binding as homo- and hetero...

journal_title：Molecular and cellular biology

authors： Molkentin JD,Black BL,Martin JF,Olson EN

更新日期：1996-06-01 00:00:00

abstract：:We have examined the expression of chimeric plasmids containing coding sequences for the herpes simplex virus thymidine kinase (tk) gene or the Tn5 gene for neomycin resistance (neo) linked to the late promoter of polyoma DNA. Although polyoma late genes are generally not expressed in transformed cells containing only...

journal_title：Molecular and cellular biology

authors： Kern FG,Basilico C

更新日期：1985-04-01 00:00:00

abstract：:Ewing's family tumor (EFT) is a rare pediatric tumor of unclear origin that occurs in bone and soft tissue. Specific chromosomal translocations found in EFT cause EWS to fuse to a subset of ets transcription factor genes (ETS), generating chimeric EWS/ETS proteins. These proteins are believed to play a crucial role in...

journal_title：Molecular and cellular biology

authors： Miyagawa Y,Okita H,Nakaijima H,Horiuchi Y,Sato B,Taguchi T,Toyoda M,Katagiri YU,Fujimoto J,Hata J,Umezawa A,Kiyokawa N

更新日期：2008-04-01 00:00:00

abstract：:NF-kappaB2 (p100/p52), a member of the NF-kappaB/Rel family of transcription factors, is involved in the regulation of a variety of genes important for immune function. Previously, we have shown that the NF-kappaB2 gene is regulated in a positive and a negative manner. Two kappaB elements within the NF-kappaB2 promote...

journal_title：Molecular and cellular biology

authors： Oswald F,Liptay S,Adler G,Schmid RM

更新日期：1998-04-01 00:00:00

abstract：:Inactivation of the tumor suppressor p53 by degradation is a mechanism utilized by cells to adapt to endoplasmic reticulum (ER) stress. However, the mechanisms of p53 destabilization by ER stress are not known. We demonstrate here that the E3 ubiquitin-ligase Hdm2 is essential for the nucleocytoplasmic transport and p...

journal_title：Molecular and cellular biology

authors： Pluquet O,Qu LK,Baltzis D,Koromilas AE

更新日期：2005-11-01 00:00:00

abstract：:Syncollin is a small protein that is abundantly expressed in pancreatic acinar cells and that is tightly associated with the lumenal side of the zymogen granule membrane. To shed light on the hitherto unknown function of syncollin, we have generated syncollin-deficient mice. The mice are viable and show a normal pancr...

journal_title：Molecular and cellular biology

authors： Antonin W,Wagner M,Riedel D,Brose N,Jahn R

更新日期：2002-03-01 00:00:00

abstract：:It has been hypothesized that protein factors may protect CpG islands from methyltransferase during development and that demethylation may involve protein-DNA interactions at demethylated sites. However, direct evidence has been lacking. In this study, demethylation at the EBNA-1 binding sites of the Epstein-Barr viru...

journal_title：Molecular and cellular biology

authors： Hsieh CL

更新日期：1999-01-01 00:00:00

abstract：:Numb proteins are evolutionarily conserved signaling molecules that make the daughter cells different after asymmetric divisions by segregating to only one daughter. They contain distinct binding motifs for alpha-adaptin (alpha-Ada) and proteins with Eps15 homology (EH) domains, which regulate endocytosis, and for E3 ...

journal_title：Molecular and cellular biology

authors： Tang H,Rompani SB,Atkins JB,Zhou Y,Osterwalder T,Zhong W

更新日期：2005-04-01 00:00:00

abstract：:Cell polarity plays a critical role in the development of all metazoans; however, the mechanisms of cell polarity and the specific role of cell polarity pathways in mammalian organisms are still poorly understood. Lethal giant larvae (Lgl) is an apical-basal polarity gene identified in Drosophila, where it functions a...

journal_title：Molecular and cellular biology

authors： Sripathy S,Lee M,Vasioukhin V

更新日期：2011-07-01 00:00:00

abstract：:The Polycomb group (PcG) of proteins represses homeotic gene expression through the assembly of multiprotein complexes on key regulatory elements. The mechanisms mediating complex assembly have remained enigmatic since most PcG proteins fail to bind DNA. We now demonstrate that the human PcG protein dinG interacts wit...

journal_title：Molecular and cellular biology

authors： Tuckfield A,Clouston DR,Wilanowski TM,Zhao LL,Cunningham JM,Jane SM

更新日期：2002-03-01 00:00:00

abstract：:In Saccharomyces cerevisiae, transcriptional activation mediated by the GAL4 regulatory protein is repressed in the absence of galactose by the binding of the GAL80 protein, an interaction that requires the carboxy-terminal 28 amino acids of GAL4. The homolog of GAL4 from Kluyveromyces lactis, LAC9, activates transcri...

journal_title：Molecular and cellular biology

authors： Salmeron JM Jr,Langdon SD,Johnston SA

更新日期：1989-07-01 00:00:00

abstract：:Protein kinase C theta (PKC theta) is unique among PKC isozymes in its translocation to the center of the immune synapse in T cells and its unique downstream signaling. Here we show that the hematopoietic protein tyrosine phosphatase (HePTP) also accumulates in the immune synapse in a PKC theta-dependent manner upon a...

journal_title：Molecular and cellular biology

authors： Nika K,Charvet C,Williams S,Tautz L,Bruckner S,Rahmouni S,Bottini N,Schoenberger SP,Baier G,Altman A,Mustelin T

更新日期：2006-03-01 00:00:00

abstract：:Abelson murine leukemia virus (A-MuLV), a retrovirus that expresses the v-abl oncogene, characteristically induces pre-B-cell lymphomas following in vivo infection of BALB/c mice or in vitro infection of suspensions of fetal liver or bone marrow cells. ABL-MYC, a retrovirus that expresses both v-abl and c-myc, induces...

journal_title：Molecular and cellular biology

authors： Weissinger EM,Mischak H,Goodnight J,Davidson WF,Mushinski JF

更新日期：1993-04-01 00:00:00

abstract：:Biochemical experiments indicate that the general transcription factor IIB (TFIIB) can interact directly with acidic activation domains and that activators can stimulate transcription by increasing recruitment of TFIIB to promoters. For promoters at which recruitment of TFIIB to promoters is limiting in vivo, one woul...

journal_title：Molecular and cellular biology

authors： Chou S,Struhl K

更新日期：1997-12-01 00:00:00

abstract：:Hydroxyurea (HU) is a DNA replication inhibitor that negatively affects both the elongation and initiation phases of replication and triggers the "intra-S phase checkpoint." Previous work with budding yeast has shown that, during a short exposure to HU, MEC1/RAD53 prevent initiation at some late S phase origins. In th...

journal_title：Molecular and cellular biology

authors： Alvino GM,Collingwood D,Murphy JM,Delrow J,Brewer BJ,Raghuraman MK

更新日期：2007-09-01 00:00:00

abstract：:Initiation of T-lymphocyte-mediated immune responses involves two cellular processes: entry into the cell cycle (G(0)-->G(1)) for clonal proliferation and coordinated changes in surface and secreted molecules that mediate effector functions. However, a point during G(0)-->G(1) beyond which T cells are committed to ent...

journal_title：Molecular and cellular biology

authors： Lea NC,Orr SJ,Stoeber K,Williams GH,Lam EW,Ibrahim MA,Mufti GJ,Thomas NS

更新日期：2003-04-01 00:00:00

abstract：:The SR protein SRp38 is a general splicing repressor that is activated by dephosphorylation during mitosis and in response to heat shock. Here we describe experiments that provide insights into the mechanism by which SRp38 functions in splicing repression. We first show that SRp38 redistributes and colocalizes with sn...

journal_title：Molecular and cellular biology

authors： Shin C,Kleiman FE,Manley JL

更新日期：2005-09-01 00:00:00

abstract：:cyt-PTP epsilon is a naturally occurring nonreceptor form of the receptor-type protein tyrosine phosphatase (PTP) epsilon. As such, cyt-PTP epsilon enables analysis of phosphatase regulation in the absence of extracellular domains, which participate in dimerization and inactivation of the receptor-type phosphatases re...

journal_title：Molecular and cellular biology

authors： Toledano-Katchalski H,Tiran Z,Sines T,Shani G,Granot-Attas S,den Hertog J,Elson A

更新日期：2003-08-01 00:00:00

abstract：:Structural maintenance of chromosomes (SMC) complexes and DNA topoisomerases are major determinants of chromosome structure and dynamics. The cohesin complex embraces sister chromatids throughout interphase, but during mitosis most cohesin is stripped from chromosome arms by early prophase, while the remaining cohesin...

journal_title：Molecular and cellular biology

authors： Tapia-Alveal C,Lin SJ,Yeoh A,Jabado OJ,O'Connell MJ

更新日期：2014-06-01 00:00:00

abstract：:Iron is an essential micronutrient for all eukaryotic organisms because it participates as a redox-active cofactor in many biological processes, including DNA replication and repair. Eukaryotic ribonucleotide reductases (RNRs) are Fe-dependent enzymes that catalyze deoxyribonucleoside diphosphate (dNDP) synthesis. We ...

journal_title：Molecular and cellular biology

authors： Sanvisens N,Romero AM,An X,Zhang C,de Llanos R,Martínez-Pastor MT,Bañó MC,Huang M,Puig S

更新日期：2014-09-01 00:00:00