Analysis of TFIIA function In vivo: evidence for a role in TATA-binding protein recruitment and gene-specific activation.


:Activation of transcription can occur by the facilitated recruitment of TFIID to promoters by gene-specific activators. To investigate the role of TFIIA in TFIID recruitment in vivo, we exploited a class of yeast TATA-binding protein (TBP) mutants that is activation and DNA binding defective. We found that co-overexpression of TOA1 and TOA2, the genes that encode yeast TFIIA, overcomes the activation defects caused by the TBP mutants. Using a genetic screen, we isolated a new class of TFIIA mutants and identified three regions on TFIIA that are likely to be involved in TBP recruitment or stabilization of the TBP-TATA complex in vivo. Amino acid replacements in only one of these regions enhance TFIIA-TBP-DNA complex formation in vitro, suggesting that the other regions are involved in regulatory interactions. To determine the relative importance of TFIIA in the regulation of different genes, we constructed yeast strains to conditionally deplete TFIIA levels prior to gene activation. While the activation of certain genes, such as INO1, was dramatically impaired by TFIIA depletion, activation of other genes, such as CUP1, was unaffected. These data suggest that TFIIA facilitates DNA binding by TBP in vivo, that TFIIA may be regulated by factors that target distinct regions of the protein, and that promoters vary significantly in the degree to which they require TFIIA for activation.


Mol Cell Biol


Liu Q,Gabriel SE,Roinick KL,Ward RD,Arndt KM




Has Abstract


1999-12-01 00:00:00












  • Identification of developmentally specific enhancers for Tsix in the regulation of X chromosome inactivation.

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    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Stavropoulos N,Rowntree RK,Lee JT

    更新日期:2005-04-01 00:00:00

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    abstract::Receptor tyrosine kinases (RTKs) play distinct roles in multiple biological systems. Many RTKs transmit similar signals, raising questions about how specificity is achieved. One potential mechanism for RTK specificity is control of the magnitude and kinetics of activation of downstream pathways. We have found that the...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Zhang SQ,Tsiaras WG,Araki T,Wen G,Minichiello L,Klein R,Neel BG

    更新日期:2002-06-01 00:00:00

  • The transcription factor RFX3 directs nodal cilium development and left-right asymmetry specification.

    abstract::There are five members of the RFX family of transcription factors in mammals. While RFX5 plays a well-defined role in the immune system, the functions of RFX1 to RFX4 remain largely unknown. We have generated mice with a deletion of the Rfx3 gene. RFX3-deficient mice exhibit frequent left-right (LR) asymmetry defects ...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Bonnafe E,Touka M,AitLounis A,Baas D,Barras E,Ucla C,Moreau A,Flamant F,Dubruille R,Couble P,Collignon J,Durand B,Reith W

    更新日期:2004-05-01 00:00:00

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    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Wang VE,Schmidt T,Chen J,Sharp PA,Tantin D

    更新日期:2004-02-01 00:00:00

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    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Mathur M,Tucker PW,Samuels HH

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    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Lanigan TM,Tverberg LA,Russo AF

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    pub_type: 杂志文章


    authors: Avivar-Valderas A,Salas E,Bobrovnikova-Marjon E,Diehl JA,Nagi C,Debnath J,Aguirre-Ghiso JA

    更新日期:2011-09-01 00:00:00

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    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Ma Y,Greider CW

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    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Duitman EH,Orinska Z,Bulanova E,Paus R,Bulfone-Paus S

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    pub_type: 杂志文章


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    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Rienzo A,Poveda-Huertes D,Aydin S,Buchler NE,Pascual-Ahuir A,Proft M

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    pub_type: 杂志文章


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    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Island ML,Mesplede T,Darracq N,Bandu MT,Christeff N,Djian P,Drouin J,Navarro S

    更新日期:2002-10-01 00:00:00

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    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Su LK,Kadesch T

    更新日期:1990-06-01 00:00:00

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    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Guertin M,LaRue H,Bernier D,Wrange O,Chevrette M,Gingras MC,Bélanger L

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    pub_type: 杂志文章


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    更新日期:2009-05-01 00:00:00

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    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Signon L,Malkova A,Naylor ML,Klein H,Haber JE

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    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Choi PM,Tchou-Wong KM,Weinstein IB

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    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Shams I,Rohmann E,Eswarakumar VP,Lew ED,Yuzawa S,Wollnik B,Schlessinger J,Lax I

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    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


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    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


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    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Amler LC,Schwab M

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  • A flexible RNA backbone within the polypyrimidine tract is required for U2AF65 binding and pre-mRNA splicing in vivo.

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    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Chen C,Zhao X,Kierzek R,Yu YT

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    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Kupiec M,Petes TD

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    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Santangelo GM,Cole CN

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    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Lei N,Heckert LL

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    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Gupta S,Plattner R,Der CJ,Stanbridge EJ

    更新日期:2000-12-01 00:00:00

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    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Ma X,Liu H,Murphy JT,Foyil SR,Godar RJ,Abuirqeba H,Weinheimer CJ,Barger PM,Diwan A

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    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: White BA,Preston GM,Lufkin TC,Bancroft C

    更新日期:1985-11-01 00:00:00

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    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Walter MJ,Park JS,Lau SK,Li X,Lane AA,Nagarajan R,Shannon WD,Ley TJ

    更新日期:2004-12-01 00:00:00