Topological requirements for homologous recombination among DNA molecules transfected into mammalian cells.

Abstract:

:Cultured animal cells rearrange foreign DNA very efficiently by homologous recombination. The individual steps that constitute the mechanism(s) of homologous recombination in transfected DNA are as yet undefined. In this study, we examined the topological requirements by using the genome of simian virus 40 (SV40) as a probe. By assaying homologous recombination between defective SV40 genomes after transfection into CV1 monkey cells, we showed that linear molecules are preferred substrates for homologous exchanges, exchanges are distributed around the SV40 genome, and the frequency of exchange is not diminished significantly by the presence of short stretches of non-SV40 DNA at the ends. These observations are considered in relation to current models of homologous recombination in mammalian cells, and a new model is proposed. The function of somatic cell recombination is discussed.

journal_name

Mol Cell Biol

authors

Wake CT,Vernaleone F,Wilson JH

doi

10.1128/mcb.5.8.2080

subject

Has Abstract

pub_date

1985-08-01 00:00:00

pages

2080-9

issue

8

eissn

0270-7306

issn

1098-5549

journal_volume

5

pub_type

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