Abstract:
:Cultured animal cells rearrange foreign DNA very efficiently by homologous recombination. The individual steps that constitute the mechanism(s) of homologous recombination in transfected DNA are as yet undefined. In this study, we examined the topological requirements by using the genome of simian virus 40 (SV40) as a probe. By assaying homologous recombination between defective SV40 genomes after transfection into CV1 monkey cells, we showed that linear molecules are preferred substrates for homologous exchanges, exchanges are distributed around the SV40 genome, and the frequency of exchange is not diminished significantly by the presence of short stretches of non-SV40 DNA at the ends. These observations are considered in relation to current models of homologous recombination in mammalian cells, and a new model is proposed. The function of somatic cell recombination is discussed.
journal_name
Mol Cell Bioljournal_title
Molecular and cellular biologyauthors
Wake CT,Vernaleone F,Wilson JHdoi
10.1128/mcb.5.8.2080subject
Has Abstractpub_date
1985-08-01 00:00:00pages
2080-9issue
8eissn
0270-7306issn
1098-5549journal_volume
5pub_type
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