Overproduction of human Myt1 kinase induces a G2 cell cycle delay by interfering with the intracellular trafficking of Cdc2-cyclin B1 complexes.

abstract：:In Saccharomyces cerevisiae, pseudouridine formation in the middle position of the tRNA(Tyr) anticodon (psi 35) is dependent on the presence of the intron in the tRNA(Tyr) gene (Johnson and Abelson, Nature 302:681-687, 1983). Drosophila melanogaster tRNA(Tyr) genes contain introns of three size classes: 20 or 21 base ...

journal_title：Molecular and cellular biology

authors： Choffat Y,Suter B,Behra R,Kubli E

更新日期：1988-08-01 00:00:00

abstract：:In Drosophila melanogaster, achaete (ac) and m8 are model basic helix-loop-helix activator (bHLH A) and repressor genes, respectively, that have the opposite cell expression pattern in proneural clusters during Notch signaling. Previous studies have shown that activation of m8 transcription in specific cells within pr...

journal_title：Molecular and cellular biology

authors： Cave JW,Xia L,Caudy M

更新日期：2011-01-01 00:00:00

abstract：:The human alpha-tropomyosin gene hTMnm has two mutually exclusive versions of exon 5 (NM and SK), one of which is expressed specifically in skeletal muscle (exon SK). A minigene construct expresses only the nonmuscle (NM) isoform when transfected into COS-1 cells and both forms when transfected into myoblasts. Twenty-...

journal_title：Molecular and cellular biology

authors： Graham IR,Hamshere M,Eperon IC

更新日期：1992-09-01 00:00:00

abstract：:Autosomal dominant polycystic kidney disease (ADPKD) constitutes the most inherited kidney disease. Mutations in the PKD1 and PKD2 genes, encoding the polycystin 1 and polycystin 2 Ca2+ ion channels, respectively, result in tubular epithelial cell-derived renal cysts. Recent clinical studies demonstrate oxidative stre...

journal_title：Molecular and cellular biology

authors： Ishimoto Y,Inagi R,Yoshihara D,Kugita M,Nagao S,Shimizu A,Takeda N,Wake M,Honda K,Zhou J,Nangaku M

更新日期：2017-11-28 00:00:00

abstract：:Xrn1p of Saccharomyces cerevisiae is a major cytoplasmic RNA turnover exonuclease which is evolutionarily conserved from yeasts to mammals. Deletion of the XRN1 gene causes pleiotropic phenotypes, which have been interpreted as indirect consequences of the RNA turnover defect. By sequence comparisons, we have identifi...

journal_title：Molecular and cellular biology

authors： Solinger JA,Pascolini D,Heyer WD

更新日期：1999-09-01 00:00:00

abstract：:TFIID, comprising the TATA box binding protein (TBP) and 13 TBP-associated factors (TAFs), plays a role in nucleation in the assembly of the RNA polymerase II preinitiation complexes on protein-encoding genes. TAFs are shared among other transcription regulatory complexes (e.g., SAGA, TBP-free TAF-containing complex [...

journal_title：Molecular and cellular biology

authors： Soutoglou E,Demény MA,Scheer E,Fienga G,Sassone-Corsi P,Tora L

更新日期：2005-05-01 00:00:00

abstract：:A DNA sequence encoding the A chain of ricin toxin (RTA) from the castor bean plant, Ricinus communis, was placed under GAL1 promoter control and transformed into Saccharomyces cerevisiae. Induction of expression of RTA was lethal. This lethality was the basis for a selection of mutations in RTA which inactivated the ...

journal_title：Molecular and cellular biology

authors： Frankel A,Schlossman D,Welsh P,Hertler A,Withers D,Johnston S

更新日期：1989-02-01 00:00:00

abstract：:During mammalian vascular development, endothelial cells form a complex array of vessels that differ markedly in structure and function, but the molecular basis for this vascular complexity is poorly understood. Recent insights into endothelial diversity have come from the identification of molecular markers expressed...

journal_title：Molecular and cellular biology

authors： Xu B,deWaal RM,Mor-Vaknin N,Hibbard C,Markovitz DM,Kahn ML

更新日期：2004-10-01 00:00:00

abstract：:To examine a role for focal adhesion kinase (FAK) in cardiac morphogenesis, we generated a line of mice with a conditional deletion of FAK in nkx2-5-expressing cells (herein termed FAKnk mice). FAKnk mice died shortly after birth, likely resulting from a profound subaortic ventricular septal defect and associated mala...

journal_title：Molecular and cellular biology

authors： Hakim ZS,DiMichele LA,Doherty JT,Homeister JW,Beggs HE,Reichardt LF,Schwartz RJ,Brackhan J,Smithies O,Mack CP,Taylor JM

更新日期：2007-08-01 00:00:00

abstract：:Transfection of a hamster cell mutant with human DNA corrected both the low-density lipoprotein receptor-deficient phenotype and the multiple glycosylation defects of the cells. Independently transfected colonies contained a small set of common human DNA fragments. These fragments may correspond to the human analog of...

journal_title：Molecular and cellular biology

authors： Kingsley DM,Sege RD,Kozarsky KF,Krieger M

更新日期：1986-07-01 00:00:00

abstract：:Centrosomes ensure accurate chromosome segregation by directing spindle bipolarity. Loss of centrosome regulation results in centrosome amplification, multipolar mitosis and aneuploidy. Since centrosome amplification is common in premalignant lesions and breast tumors, it is proposed to play a central role in breast t...

journal_title：Molecular and cellular biology

authors： Lee MY,Moreno CS,Saavedra HI

更新日期：2014-07-01 00:00:00

abstract：:Human myeloid leukemia cells respond to 12-O-tetradecanoylphorbol-13-acetate (TPA) and other activators of protein kinase C (PKC) with induction of monocytic differentiation. The present studies demonstrated that treatment of U-937 and HL-60 myeloid leukemia cells with TPA, phorbol-12,13-dibutyrate, or bryostatin 1 wa...

journal_title：Molecular and cellular biology

authors： Kaneki M,Kharbanda S,Pandey P,Yoshida K,Takekawa M,Liou JR,Stone R,Kufe D

更新日期：1999-01-01 00:00:00

abstract：:The biological response to progesterone is mediated by two distinct forms of the human progesterone receptor (hPR-A and hPR-B). In most cell contexts, hPR-B functions as a transcriptional activator of progesterone-responsive genes, whereas hPR-A functions as a transcriptional inhibitor of all steroid hormone receptors...

journal_title：Molecular and cellular biology

authors： Wen DX,Xu YF,Mais DE,Goldman ME,McDonnell DP

更新日期：1994-12-01 00:00:00

abstract：:The oxidation of methanol follows a well-defined pathway and is similar for several methylotrophic yeasts. The use of methanol as the sole carbon source for the growth of Pichia pastoris stimulates the expression of a family of genes. Three methanol-responsive genes have been isolated; cDNA copies have been made from ...

journal_title：Molecular and cellular biology

authors： Ellis SB,Brust PF,Koutz PJ,Waters AF,Harpold MM,Gingeras TR

更新日期：1985-05-01 00:00:00

abstract：:Chicken embryo fibroblast cells overexpressing activated mutant forms of human pp60c-src, but not those overexpressing normal human pp60c-src, exhibited high levels of type I phosphatidylinositol (PI) kinase activity associated with pp60c-src. Levels of PI kinase activity were positively correlated with src tyrosine p...

journal_title：Molecular and cellular biology

authors： Chan TO,Tanaka A,Bjorge JD,Fujita DJ

更新日期：1990-06-01 00:00:00

abstract：:The M double-stranded RNA component of type 1 killer strains of the yeast Saccharomyces cerevisiae contains an internal 200-base pair adenine- and uracil-rich region. The plus strands of this viral genomic RNA contain an internal adenine-rich region which allows these strands to bind to polyuridylate-Sepharose as tigh...

journal_title：Molecular and cellular biology

authors： Hannig EM,Thiele DJ,Leibowitz MJ

更新日期：1984-01-01 00:00:00

abstract：:Yeast cells are keenly sensitive to the availability and quality of nutrients. Addition of glucose to cells growing on a poorer carbon source elicits a cell cycle delay during G1 phase and a concomitant increase in the cell size. The signal is transduced through the RAS-cyclic AMP pathway. Using synchronized populatio...

journal_title：Molecular and cellular biology

authors： Flick K,Chapman-Shimshoni D,Stuart D,Guaderrama M,Wittenberg C

更新日期：1998-05-01 00:00:00

abstract：:ADP-ribosyltransferase diphtheria-toxin like 1/poly(ADP-ribose) polymerase 1 (ARTD1/PARP1) is a chromatin-associated protein in the nucleus and plays an important role in different cellular processes such as regulation of gene transcription. ARTD1 has been shown to coregulate the inflammatory response by modulating th...

journal_title：Molecular and cellular biology

authors： Minotti R,Andersson A,Hottiger MO

更新日期：2015-09-01 00:00:00

abstract：:The exons, their boundaries, and approximately half of the intronic deoxyribonucleic acid of the rat serum albumin gene were sequenced. In addition to the 14 exons identified earlier by R-loop analysis, a small exon was detected between the "leader" exon (Z) and exon B. The leader exon encoded the 5'-untranslated port...

journal_title：Molecular and cellular biology

authors： Sargent TD,Jagodzinski LL,Yang M,Bonner J

更新日期：1981-10-01 00:00:00

abstract：:Deacetylation of histones is carried out by a corepressor complex in which Sin3A is an essential scaffold protein. Two proteins in this complex, the Sin3A-associated proteins SAP30L and SAP30, have previously been suggested to function as linker molecules between various corepressors. In this report, we demonstrate ne...

journal_title：Molecular and cellular biology

authors： Viiri KM,Jänis J,Siggers T,Heinonen TY,Valjakka J,Bulyk ML,Mäki M,Lohi O

更新日期：2009-01-01 00:00:00

abstract：:The regulation of telomerase reverse transcriptase (TERT) plays an important role in the proliferative capacity and survival of cells. Here, we report that exogenously as well as endogenously induced oxidative stress leads to translocation of endogenous as well as overexpressed human TERT from the nucleus into the cyt...

journal_title：Molecular and cellular biology

authors： Haendeler J,Hoffmann J,Brandes RP,Zeiher AM,Dimmeler S

更新日期：2003-07-01 00:00:00

abstract：:Centrins in vertebrates have traditionally been associated with microtubule-nucleating centers such as the centrosome. Unexpectedly, we found centrin 2 to associate biochemically with nucleoporins, including the Xenopus laevis Nup107-160 complex, a critical subunit of the vertebrate nuclear pore in interphase and of t...

journal_title：Molecular and cellular biology

authors： Resendes KK,Rasala BA,Forbes DJ

更新日期：2008-03-01 00:00:00

abstract：:Essential for embryonic development, the polycomb group protein enhancer of zeste homolog 2 (EZH2) is overexpressed in breast and prostate cancers and is implicated in the growth and aggression of the tumors. The tumorigenic mechanism underlying EZH2 overexpression is largely unknown. It is believed that EZH2 exerts i...

journal_title：Molecular and cellular biology

authors： Shi B,Liang J,Yang X,Wang Y,Zhao Y,Wu H,Sun L,Zhang Y,Chen Y,Li R,Zhang Y,Hong M,Shang Y

更新日期：2007-07-01 00:00:00

abstract：:p53 promotes apoptosis in response to death stimuli by transactivation of target genes and by transcription-independent mechanisms. We recently showed that wild-type p53 rapidly translocates to mitochondria in response to multiple death stimuli in cultured cells. Mitochondrial p53 physically interacts with antiapoptot...

journal_title：Molecular and cellular biology

authors： Erster S,Mihara M,Kim RH,Petrenko O,Moll UM

更新日期：2004-08-01 00:00:00

abstract：:V(D)J recombination requires binding and synapsis of a complementary (12/23) pair of recombination signal sequences (RSSs) by the RAG1 and RAG2 proteins, aided by a high-mobility group protein, HMG1 or HMG2. Double-strand DNA cleavage within this synaptic, or paired, complex is thought to involve DNA distortion or mel...

journal_title：Molecular and cellular biology

authors： Ciubotaru M,Schatz DG

更新日期：2004-10-01 00:00:00

abstract：:Nutrients, drugs, and hormones influence transcription during differentiation and metabolism by binding to high-affinity nuclear receptors. In the absence of ligand, some but not all nuclear receptors repress transcription as a heterodimer with retinoid X receptor (RXR). Here we define a novel role for helix 12 (H12) ...

journal_title：Molecular and cellular biology

authors： Zhang J,Hu X,Lazar MA

更新日期：1999-09-01 00:00:00

abstract：:tRNAs in eukaryotic nuclei and organelles are synthesized as precursors lacking the 3'-terminal CCA sequence and possessing 5' (leader) and 3' (trailer) extensions. Nucleolytic cleavage of the 3' trailer and addition of CCA are therefore required for formation of functional tRNA 3' termini. Many chloroplast tRNA genes...

journal_title：Molecular and cellular biology

authors： Oommen A,Li XQ,Gegenheimer P

更新日期：1992-02-01 00:00:00

abstract：:We reported the identification of a new family of DNA-binding proteins from our characterization of the dead ringer (dri) gene of Drosophila melanogaster. We show that dri encodes a nuclear protein that contains a sequence-specific DNA-binding domain that bears no similarity to known DNA-binding domains. A number of p...

journal_title：Molecular and cellular biology

authors： Gregory SL,Kortschak RD,Kalionis B,Saint R

更新日期：1996-03-01 00:00:00

abstract：:The effect of atenolol, propranolol, trifluoperazine, and caffeine on the occurrence of meiotic diploid and disomic products in Saccharomyces cerevisiae was investigated. We demonstrated that atenolol, propranolol, and trifluoperazine reduce the occurrence of meiotic diploid products and that propranolol also slightly...

journal_title：Molecular and cellular biology

authors： Sora S,Bianchi M

更新日期：1982-11-01 00:00:00

abstract：:The distal region (-830 to -720 bp) of the rat whey acidic protein (WAP) gene contains a composite response element (CoRE), which has been demonstrated previously to confer mammary gland-specific and hormonally regulated WAP gene expression. Point mutations in the binding sites for specific transcription factors prese...

journal_title：Molecular and cellular biology

authors： Mukhopadhyay SS,Wyszomierski SL,Gronostajski RM,Rosen JM

更新日期：2001-10-01 00:00:00