Abstract:
:The class II transactivator (CIITA) is induced by gamma interferon (IFN-gamma) and activates major histocompatibility complex class II; however, this report shows it suppresses other genes. An N-terminal 36 amino acids of CIITA mediates suppression of the collagen alpha(2)(I) promoter via binding to CREB-binding protein (CBP). Reconstitution of cells with CBP reverts this suppression. IFN-gamma is known to inhibit collagen gene expression; to test if CIITA mediates this gene suppression, a mutant cell line defective in CIITA induction but not in the activation of STAT1/JAK/IRF-1 is studied. IFN-gamma suppression of the collagen promoter and the endogenous gene is observed in the wild-type control but not in the mutant line. Suppression is restored when CIITA is introduced. Other targets of CIITA-mediated promoter suppression include interleukin 4, thymidine kinase, and cyclin D1.
journal_name
Mol Cell Bioljournal_title
Molecular and cellular biologyauthors
Zhu XS,Ting JPdoi
10.1128/MCB.21.20.7078-7088.2001subject
Has Abstractpub_date
2001-10-01 00:00:00pages
7078-88issue
20eissn
0270-7306issn
1098-5549journal_volume
21pub_type
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