The major myelin-resident protein PLP is transported to myelin membranes via a transcytotic mechanism: involvement of sulfatide.

abstract：:We have screened an adult rat cerebellar cDNA library in search of novel protein tyrosine-kinase (PTK) cDNAs. A cDNA for a putative PTK, trkB, was cloned, and its sequence indicates that it is likely to be derived from a gene for a ligand-regulated receptor closely related to the human trk oncogene. Northern (RNA) ana...

journal_title：Molecular and cellular biology

authors： Middlemas DS,Lindberg RA,Hunter T

更新日期：1991-01-01 00:00:00

abstract：:The RNA polymerase III transcription initiation factor TFIIIB contains the TATA-box-binding protein (TBP) and polymerase III-specific TBP-associated factors (TAFs). Previous studies have shown that DNA oligonucleotides containing the consensus TATA-box sequence inhibit polymerase III transcription, implying that the D...

journal_title：Molecular and cellular biology

authors： McBryant SJ,Meier E,Leresche A,Sharp SJ,Wolf VJ,Gottesfeld JM

更新日期：1996-09-01 00:00:00

abstract：:Most mammalian genes produce transcripts whose 3' ends are processed at multiple alternative positions by cleavage/polyadenylation (CPA). Poly(A) site cleavage frequently occurs cotranscriptionally and is facilitated by CPA factor binding to the RNA polymerase II (Pol II) C-terminal domain (CTD) phosphorylated on Ser2...

journal_title：Molecular and cellular biology

authors： Fusby B,Kim S,Erickson B,Kim H,Peterson ML,Bentley DL

更新日期：2015-11-02 00:00:00

abstract：:Transforming growth factor beta (TGF-beta) plays important roles in the regulation of proliferation, differentiation, apoptosis, and carcinogenesis. To identify genes responsible for maintaining the phenotype induced by TGF-beta, we performed a retrovirus-mediated gene trap screening designed to isolate TGF-beta-respo...

journal_title：Molecular and cellular biology

authors： Akiyama N,Matsuo Y,Sai H,Noda M,Kizaka-Kondoh S

更新日期：2000-05-01 00:00:00

abstract：:Hox gene functions are intimately linked to correct developmental expression of the genes. The identification of cis-acting regulatory sequences and their associated trans-acting factors constitutes a key step in deciphering the mechanisms underlying the correct positioning of the functional domain of Hox genes along ...

journal_title：Molecular and cellular biology

authors： Tabariès S,Lapointe J,Besch T,Carter M,Woollard J,Tuggle CK,Jeannotte L

更新日期：2005-02-01 00:00:00

abstract：:The LAR family protein tyrosine phosphatases (PTPs), including LAR, PTP delta, and PTP sigma, are transmembrane proteins composed of a cell adhesion molecule-like ectodomain and two cytoplasmic catalytic domains: active D1 and inactive D2. We performed a yeast two-hybrid screen with the first catalytic domain of PTP s...

journal_title：Molecular and cellular biology

authors： Wallace MJ,Fladd C,Batt J,Rotin D

更新日期：1998-05-01 00:00:00

abstract：:The two beta-tubulin genes of the unicellular green alga Chlamydomonas reinhardtii are expressed coordinately after deflagellation and produce two transcripts of 2.1 and 2.0 kilobases. Full-length cDNA clones corresponding to the transcript of each gene were isolated. DNA sequences were obtained from the cDNA clones a...

journal_title：Molecular and cellular biology

authors： Youngblom J,Schloss JA,Silflow CD

更新日期：1984-12-01 00:00:00

abstract：:LFB1 (HNF1) is a tissue-specific transcription factor found in the livers, stomachs, intestines, and kidneys of vertebrates. By analyzing the promoter of the Xenopus LFB1 gene, we identified potential autoregulation by LFB1 and regulation by HNF4, a transcription factor with a tissue distribution similar to that of LF...

journal_title：Molecular and cellular biology

authors： Zapp D,Bartkowski S,Holewa B,Zoidl C,Klein-Hitpass L,Ryffel GU

更新日期：1993-10-01 00:00:00

abstract：:The DNA single-strand break repair (SSBR) protein XRCC1 is required for genetic stability and for embryonic viability. XRCC1 possesses two BRCA1 carboxyl-terminal (BRCT) protein interaction domains, denoted BRCT I and II. BRCT II is required for SSBR during G(1) but is dispensable for this process during S/G(2) and co...

journal_title：Molecular and cellular biology

authors： Taylor RM,Thistlethwaite A,Caldecott KW

更新日期：2002-04-01 00:00:00

abstract：:We have isolated a yeast conditional mutant which rapidly ceases synthesis of mRNA when subjected to the nonpermissive temperature. This mutant (rpb1-1) was constructed by replacing the wild-type chromosomal copy of the gene encoding the largest subunit of RNA polymerase II with one mutagenized in vitro. The rapid ces...

journal_title：Molecular and cellular biology

authors： Nonet M,Scafe C,Sexton J,Young R

更新日期：1987-05-01 00:00:00

abstract：:Rats and mice have two, equally expressed, nonallelic genes encoding preproinsulin (genes I and II). Cytological hybridization with metaphase chromosomes indicated that both genes reside on rat chromosome I but are approximately 100,000 kilobases apart. In mice the two genes reside on two different chromosomes. DNA se...

journal_title：Molecular and cellular biology

authors： Soares MB,Schon E,Henderson A,Karathanasis SK,Cate R,Zeitlin S,Chirgwin J,Efstratiadis A

更新日期：1985-08-01 00:00:00

abstract：:We have isolated new histone H3 mutants in Saccharomyces cerevisiae that confer phenotypes indicative of transcriptional defects. Here we describe the characterization of one such mutant, encoded by the hht2-11 allele, which contains the single amino acid change L61W in the globular domain of H3. Whole-genome expressi...

journal_title：Molecular and cellular biology

authors： Duina AA,Winston F

更新日期：2004-01-01 00:00:00

abstract：:Transcriptional silencing in Saccharomyces cerevisiae occurs at specific loci and is mediated by a multiprotein complex that includes Rap1p and the Sir proteins. We studied the function of a recently identified gene, DOT4, that disrupts silencing when overexpressed. DOT4 encodes an ubiquitin processing protease (hydro...

journal_title：Molecular and cellular biology

authors： Kahana A,Gottschling DE

更新日期：1999-10-01 00:00:00

abstract：:Aberrant glycogen synthase kinase 3beta (GSK-3beta) activity is associated with the progression of several pathological conditions such as diabetes, Alzheimer's, and cancer. GSK-3beta regulates cellular processes by directly phosphorylating metabolic enzymes and transcription factors. Here, we discovered a new target ...

journal_title：Molecular and cellular biology

authors： Galliher-Beckley AJ,Williams JG,Collins JB,Cidlowski JA

更新日期：2008-12-01 00:00:00

abstract：:Apoptotic cells are swiftly engulfed by macrophages to prevent the release of noxious materials from dying cells. Apoptotic cells expose phosphatidylserine (PtdSer) on their surface, and macrophages engulf them by recognizing PtdSer using specific receptors and opsonins. Here, we found that mouse resident peritoneal m...

journal_title：Molecular and cellular biology

authors： Nishi C,Toda S,Segawa K,Nagata S

更新日期：2014-04-01 00:00:00

abstract：:Metazoan replication-dependent histone mRNAs are the only eukaryotic mRNAs that are not polyadenylated. The cleavage of histone pre-mRNA to form the unique 3' end requires the U7 snRNP and the stem-loop binding protein (SLBP) that binds the 3' end of histone mRNA. U7 snRNP contains three novel proteins, Lsm10 and Lsm1...

journal_title：Molecular and cellular biology

authors： Wagner EJ,Marzluff WF

更新日期：2006-09-01 00:00:00

abstract：:Changes in chromatin composition are often a prerequisite for gene induction. Nonallelic histone variants have recently emerged as key players in transcriptional control and chromatin modulation. While the changes in chromatin accessibility and histone posttranslational modification (PTM) distribution that accompany g...

journal_title：Molecular and cellular biology

authors： Sutcliffe EL,Parish IA,He YQ,Juelich T,Tierney ML,Rangasamy D,Milburn PJ,Parish CR,Tremethick DJ,Rao S

更新日期：2009-04-01 00:00:00

abstract：:Etoposide, a nonintercalating antitumor drug, is a potent inhibitor of topoisomerase II activity. When Trypanosoma equiperdum is treated with etoposide, cleavable complexes are stabilized between topoisomerase II and kinetoplast DNA minicircles, a component of trypanosome mitochondrial DNA (T. A. Shapiro, V. A. Klein,...

journal_title：Molecular and cellular biology

authors： Shapiro TA

更新日期：1994-06-01 00:00:00

abstract：:Two Drosophila tumor necrosis factor receptor-associated factors (TRAF), DTRAF1 and DTRAF2, are proposed to have similar functions with their mammalian counterparts as a signal mediator of cell surface receptors. However, their in vivo functions and related signaling pathways are not fully understood yet. Here, we sho...

journal_title：Molecular and cellular biology

authors： Cha GH,Cho KS,Lee JH,Kim M,Kim E,Park J,Lee SB,Chung J

更新日期：2003-11-01 00:00:00

abstract：:An ACG codon, which replaces the AUG codon used to initiate the synthesis of bacteriophage T7 gene 0.3 protein, was shown to function as a low-efficiency initiation codon in a wheat germ cell-free protein-synthesizing system. ...

journal_title：Molecular and cellular biology

authors： Anderson CW,Buzash-Pollert E

更新日期：1985-12-01 00:00:00

abstract：:Previous studies have shown that exposure of cells to high levels of replicational stress leads to permanent proliferation arrest that does not require p53. We have examined cellular responses to therapeutically relevant low levels of replicational stress that allow limited proliferation. Chronic exposure to low conce...

journal_title：Molecular and cellular biology

authors： Marusyk A,Wheeler LJ,Mathews CK,DeGregori J

更新日期：2007-08-01 00:00:00

abstract：:In Blastocladiella emersonii zoospores, a set of proteins was found associated with the ribosomes and free ribonucleoprotein particles distinct from the ribosomes and polyribosomes. These proteins were designated P120, P105, P64, P56, and P42 based on their molecular weights determined by gel electrophoresis. Synthesi...

journal_title：Molecular and cellular biology

authors： Jaworski AJ,Stumhofer P

更新日期：1981-04-01 00:00:00

abstract：:Saccharomyces mating-type switching occurs through a double-strand break-initiated gene conversion event at MAT, using one of two donors located distantly on the same chromosome, HMLalpha and HMRa. MATa cells preferentially choose HMLalpha, a decision that depends on the recombination enhancer (RE) that controls recom...

journal_title：Molecular and cellular biology

authors： Coïc E,Sun K,Wu C,Haber JE

更新日期：2006-07-01 00:00:00

abstract：:Structural maintenance of chromosomes (SMC) complexes and DNA topoisomerases are major determinants of chromosome structure and dynamics. The cohesin complex embraces sister chromatids throughout interphase, but during mitosis most cohesin is stripped from chromosome arms by early prophase, while the remaining cohesin...

journal_title：Molecular and cellular biology

authors： Tapia-Alveal C,Lin SJ,Yeoh A,Jabado OJ,O'Connell MJ

更新日期：2014-06-01 00:00:00

abstract：:The steroid hormone progesterone acts via high-affinity nuclear receptors that interact with specific DNA sequences located near the promoter of the hormone-responsive gene. Recent studies suggested that the hormone-occupied progesterone receptor (PR) mediates gene activation by recruiting a cellular coregulatory fact...

journal_title：Molecular and cellular biology

authors： Xu Y,Klein-Hitpass L,Bagchi MK

更新日期：2000-03-01 00:00:00

abstract：:The ligand-binding domains of thyroid hormone (L-triiodothyronine [T3]) receptors (T3Rs), all-trans retinoic acid (RA) receptors (RARs), and 9-cis RA receptors (RARs and RXRs) contain a series of heptad motifs thought to be important for dimeric interactions. Using a chimera containing amino acids 120 to 392 of chicke...

journal_title：Molecular and cellular biology

authors： Casanova J,Helmer E,Selmi-Ruby S,Qi JS,Au-Fliegner M,Desai-Yajnik V,Koudinova N,Yarm F,Raaka BM,Samuels HH

更新日期：1994-09-01 00:00:00

abstract：:Efficient cleavage of both forms of eukaryotic initiation factor 4G (eIF4G-1 and eIF4G-2) has been achieved in HeLa cells by incubation with hybrid proteins containing poliovirus 2Apro. Entry of these proteins into cells is promoted by adenovirus particles. Substantial levels of ongoing translation on preexisting cell...

journal_title：Molecular and cellular biology

authors： Novoa I,Carrasco L

更新日期：1999-04-01 00:00:00

abstract：:The retinoblastoma protein (RB) represses RNA polymerase III transcription effectively both in vivo and in vitro. Here we demonstrate that the general transcription factors snRNA-activating protein complex (SNAP(c)) and TATA binding protein (TBP) are important for RB repression of human U6 snRNA gene transcription by ...

journal_title：Molecular and cellular biology

authors： Hirsch HA,Gu L,Henry RW

更新日期：2000-12-01 00:00:00

abstract：:ING1 is a type II tumor suppressor that affects cell growth, stress signaling, apoptosis, and DNA repair by altering chromatin structure and regulating transcription. Decreased ING1 expression is seen in several human cancers, and mislocalization has been noted in diverse types of cancer cells. Aberrant targeting may,...

journal_title：Molecular and cellular biology

authors： Gong W,Russell M,Suzuki K,Riabowol K

更新日期：2006-04-01 00:00:00

abstract：:We report a negative feedback loop between the signaling protein phospholipase D (PLD), phosphatidic acid (PA), and a specific set of microRNAs (miRNAs) during nutrient starvation of breast cancer cells. We show that PLD expression is increased in four breast cancer cell lines and that hypoxia, cell overcrowding, and ...

journal_title：Molecular and cellular biology

authors： Fite K,Elkhadragy L,Gomez-Cambronero J

更新日期：2016-01-19 00:00:00