Discrete functions of TRAF1 and TRAF2 in Drosophila melanogaster mediated by c-Jun N-terminal kinase and NF-kappaB-dependent signaling pathways.

abstract：:Binding sites for six distinct nuclear factors on the 75-base-pair repeat of the Moloney murine leukemia virus enhancer have been identified by an electrophoretic mobility shift assay combined with methylation interference. Three of these factors, found in WEHI 231 nuclear extracts, which we have named LVa, LVb, and L...

journal_title：Molecular and cellular biology

authors： Speck NA,Baltimore D

更新日期：1987-03-01 00:00:00

abstract：:In Drosophila melanogaster, X chromosome dosage compensation is achieved by doubling the transcription of most X-linked genes. The male-specific lethal (MSL) complex is required for this process and binds to hundreds of sites on the male X chromosome. The MSL1 protein is essential for X chromosome binding and serves a...

journal_title：Molecular and cellular biology

authors： Li F,Parry DA,Scott MJ

更新日期：2005-10-01 00:00:00

abstract：:To examine the internal organization of the promoter of the Xenopus laevis rRNA gene, we constructed a series of linker-scanning mutants that traverse the rDNA initiation region. The mutant genes, which have 3 to 11 clustered base substitutions set within an otherwise unaltered rDNA promoter sequence, were injected in...

journal_title：Molecular and cellular biology

authors： Windle JJ,Sollner-Webb B

更新日期：1986-12-01 00:00:00

abstract：:Reb1p is a DNA binding protein of Saccharomyces cerevisiae that has been implicated in the activation of transcription by polymerase (Pol) II, in the termination of transcription by Pol I, and in the organization of nucleosomes. Studies of the transcriptional control of the REB1 gene have led us to identify three Reb1...

journal_title：Molecular and cellular biology

authors： Wang KL,Warner JR

更新日期：1998-07-01 00:00:00

abstract：:We investigated the interaction of phospholipase C-gamma (PLC-gamma) with wild-type and mutant forms of the platelet-derived growth factor (PDGF) beta-receptor both in vivo and in vitro. After PDGF treatment of CHO cell lines expressing wild-type or either of two mutant (delta Ki and Y825F) PDGF receptors, PLC-gamma b...

journal_title：Molecular and cellular biology

authors： Morrison DK,Kaplan DR,Rhee SG,Williams LT

更新日期：1990-05-01 00:00:00

abstract：:Tumor-promoting phorbol esters affect a variety of cellular functions which may underlie tumor promotion. We isolated from human lymphocytes a cDNA clone whose gene is inducible by the phorbol ester 12-O-tetradecanoyl-phorbol-13-acetate as well as by the T-cell mitogen phytohemagglutinin. Nuclear transcription experim...

journal_title：Molecular and cellular biology

authors： Arya SK,Wong-Staal F,Gallo RC

更新日期：1984-11-01 00:00:00

abstract：:We report the isolation and molecular characterization of the mouse grb2 gene. The product of this gene, the Grb2 protein, is highly related to the Caenorhabditis elegans sem-5 gene product and the human GRB2 protein and displays the same SH3-SH2-SH3 structural motifs. In situ hybridization studies revealed that the m...

journal_title：Molecular and cellular biology

authors： Suen KL,Bustelo XR,Pawson T,Barbacid M

更新日期：1993-09-01 00:00:00

abstract：:We have analyzed the effects of transformation by Rous sarcoma virus on expression of types I and II collagen and fibronectin genes in vertebral chondrocytes and compared them with expression of these genes in skin fibroblasts. Transformed chondrocytes display a dramatically decreased amount of type II collagen RNA, w...

journal_title：Molecular and cellular biology

authors： Allebach ES,Boettiger D,Pacifici M,Adams SL

更新日期：1985-05-01 00:00:00

abstract：:We provide evidence that the human DNA ligase III gene encodes a mitochondrial form of this enzyme. First, the DNA ligase III cDNA contains an in-frame ATG located upstream from the putative translation initiation start site. The DNA sequence between these two ATG sites encodes an amphipathic helix similar to previous...

journal_title：Molecular and cellular biology

authors： Lakshmipathy U,Campbell C

更新日期：1999-05-01 00:00:00

abstract：:The roles of PDZ domain-containing proteins such as Dlg and Scrib have been well described for Drosophila; however, their requirement for mammalian development is poorly understood. Here we show that Dlg, Scrib, MAGI1, MAGI3, and MPDZ are expressed in the mouse ocular lens. We demonstrate that the increase in prolifer...

journal_title：Molecular and cellular biology

authors： Nguyen MM,Nguyen ML,Caruana G,Bernstein A,Lambert PF,Griep AE

更新日期：2003-12-01 00:00:00

abstract：:The polypyrimidine tract near the 3' splice site is important for pre-mRNA splicing. Using pseudouridine incorporation and in vivo RNA-guided RNA pseudouridylation, we have identified two important uridines in the polypyrimidine tract of adenovirus pre-mRNA. Conversion of either uridine into pseudouridine leads to a s...

journal_title：Molecular and cellular biology

authors： Chen C,Zhao X,Kierzek R,Yu YT

更新日期：2010-09-01 00:00:00

abstract：:Five mouse alpha-tubulin isotypes are described, each distinguished by the presence of unique amino acid substitutions within the coding region. Most, though not all of these isotype-specific amino acids, are clustered at the carboxy terminus. One of the alpha-tubulin isotypes described is expressed exclusively in tes...

journal_title：Molecular and cellular biology

authors： Villasante A,Wang D,Dobner P,Dolph P,Lewis SA,Cowan NJ

更新日期：1986-07-01 00:00:00

abstract：:Retinoic acid is known to have profound effects on developmental processes. It has been implicated as a putative morphogen in the developing chick limb bud and regenerating amphibian limb blastema and has been demonstrated to have powerful teratogenic effects in mammals, including humans. Recently, three specific reti...

journal_title：Molecular and cellular biology

authors： Giguère V,Shago M,Zirngibl R,Tate P,Rossant J,Varmuza S

更新日期：1990-05-01 00:00:00

abstract：:TBC (Tre-2/Bub2/Cdc16) domains are predicted to encode GTPase-activating proteins (GAPs) for Rab family G proteins. While approximately 50 TBC proteins are predicted to exist in humans, little is known about their substrate specificity. Here we show that TRE17 (also called Tre-2 and USP6), a founding member of the TBC...

journal_title：Molecular and cellular biology

authors： Martinu L,Masuda-Robens JM,Robertson SE,Santy LC,Casanova JE,Chou MM

更新日期：2004-11-01 00:00:00

abstract：:Host cell cycle genes provide important functions to retroviruses and retroviruslike elements. To define some of these functions, the cell cycle dependence of transposition of the yeast retroviruslike element Ty3 was examined. Ty3 is unique among retroviruslike elements because of the specificity of its integration, w...

journal_title：Molecular and cellular biology

authors： Menees TM,Sandmeyer SB

更新日期：1994-12-01 00:00:00

abstract：:In order to identify catalytically important amino acid changes within the second-largest subunit of yeast RNA polymerase III, we mutagenized selected regions of its gene (RET1) and devised in vivo assays for both increased and decreased transcription termination by this enzyme. Using as the reporter gene a mutant SUP...

journal_title：Molecular and cellular biology

authors： Shaaban SA,Krupp BM,Hall BD

更新日期：1995-03-01 00:00:00

abstract：:In Caenorhabditis elegans, the central cell-killing process is essentially controlled by the interplay of four apoptotic factors: EGL-1/BH3-only protein, CED-9/Bcl2, CED-4/Apaf1, and CED-3/caspase. In cells destined to die, EGL-1 binds to CED-9 and results in the release of CED-4 from the mitochondrion-tethered CED-9-...

journal_title：Molecular and cellular biology

authors： Shen Q,Qin F,Gao Z,Cui J,Xiao H,Xu Z,Yang C

更新日期：2009-07-01 00:00:00

abstract：:The Swi5 zinc finger and the Pho2 homeodomain DNA-binding proteins bind cooperatively to the HO promoter. Pho2 (also known as Bas2 or Grf10) activates transcription of diverse genes, acting with multiple distinct DNA-binding proteins. We have performed a genetic screen to identify amino acid residues in Swi5 that are ...

journal_title：Molecular and cellular biology

authors： Bhoite LT,Stillman DJ

更新日期：1998-11-01 00:00:00

abstract：:Costimulation of G protein-coupled receptors (GPCRs) may result in cross talk interactions between their downstream signaling pathways. Stimulation of GPCRs may also lead to cross talk regulation of receptor tyrosine kinase signaling and thereby to activation of mitogen-activated protein kinase (MAPK). In COS-7 cells,...

journal_title：Molecular and cellular biology

authors： Hanke S,Nürnberg B,Groll DH,Liebmann C

更新日期：2001-12-01 00:00:00

abstract：:The histone H1(0)-encoding gene is expressed in vertebrates in differentiating cells during the arrest of proliferation. In the H1(0) promoter, a specific regulatory element, which we named the H4 box, exhibits features which implicate a role in mediating H1(0) gene expression in response to both differentiation and c...

journal_title：Molecular and cellular biology

authors： Lemercier C,Duncliffe K,Boibessot I,Zhang H,Verdel A,Angelov D,Khochbin S

更新日期：2000-09-01 00:00:00

abstract：:Eight strains of Chinese hamster ovary (CHO) cells having an assembly-defective beta-tubulin were found among revertants of strain Cmd 4, a mutant with a conditional lethal mutation in a beta-tubulin gene (F. Cabral, M. E. Sobel, and M. M. Gottesman, Cell 20:29-36, 1980). The altered beta-tubulins in these strains hav...

journal_title：Molecular and cellular biology

authors： Boggs B,Cabral F

更新日期：1987-08-01 00:00:00

abstract：:In Saccharomyces cerevisiae, the intracellular concentration of trehalose increases rapidly in response to many environmental stresses, including heat shock. These high trehalose levels have been correlated with tolerance to adverse conditions and led to the model that trehalose functions as a chemical cochaperone. He...

journal_title：Molecular and cellular biology

authors： Conlin LK,Nelson HC

更新日期：2007-02-01 00:00:00

abstract：:Three DNA polymerases, polymerases α, δ, and ε (Pol α, Pol δ, and Pol ε), are responsible for eukaryotic genome duplication. When DNA replication stress is encountered, DNA synthesis stalls until the stress is ameliorated. However, it is not known whether there is a difference in the association of each polymerase wit...

journal_title：Molecular and cellular biology

authors： Yu C,Gan H,Zhang Z

更新日期：2017-10-13 00:00:00

abstract：:The Notch receptor controls development by activating transcription of specific target genes in response to extracellular signals. The factors that control assembly of the Notch activator complex on target genes and its ability to activate transcription are not fully known. Here we show, through genetic and molecular ...

journal_title：Molecular and cellular biology

authors： Gause M,Eissenberg JC,Macrae AF,Dorsett M,Misulovin Z,Dorsett D

更新日期：2006-03-01 00:00:00

abstract：:We have analyzed the gene-targeting frequencies and recombination products generated by a series of vectors which target the hprt locus in embryonic stem cells and found the existence of alternative pathways that depend on the location of the double-strand break within the vector. A double-strand break in the targetin...

journal_title：Molecular and cellular biology

authors： Hasty P,Rivera-Pérez J,Bradley A

更新日期：1992-06-01 00:00:00

abstract：:We have previously shown that the SIS/platelet-derived growth factor B chain contains a nuclear targeting signal near its C terminus. Here we show that the platelet-derived growth factor A chain also contains a nuclear targeting signal encoded by an exon which is subject to alternative splicing. This sequence is capab...

journal_title：Molecular and cellular biology

authors： Maher DW,Lee BA,Donoghue DJ

更新日期：1989-05-01 00:00:00

abstract：:RSC is an essential chromatin remodeling complex in Saccharomyces cerevisiae that performs central roles in transcriptional regulation and cell cycle progression. Here we identify Htl1 as a novel factor that associates with the RSC complex both physically and functionally. We isolated HTL1 through a genetic screen for...

journal_title：Molecular and cellular biology

authors： Romeo MJ,Angus-Hill ML,Sobering AK,Kamada Y,Cairns BR,Levin DE

更新日期：2002-12-01 00:00:00

abstract：:The RAD10 gene of Saccharomyces cerevisiae is required for the incision step of excision repair of UV-damaged DNA. We show that the RAD10 gene is also required for mitotic recombination. The rad10 delta mutation lowered the rate of intrachromosomal recombination of a his3 duplication in which one his3 allele has a del...

journal_title：Molecular and cellular biology

authors： Schiestl RH,Prakash S

更新日期：1990-06-01 00:00:00

abstract：:The gene encoding the 180-kDa DNA strand transfer protein beta from the yeast Saccharomyces cerevisiae was identified and sequenced. This gene, DST2 (DNA strand transferase 2), was located on chromosome VII. dst2 gene disruption mutants exhibited temperature-sensitive sporulation and a 50% longer generation time durin...

journal_title：Molecular and cellular biology

authors： Dykstra CC,Kitada K,Clark AB,Hamatake RK,Sugino A

更新日期：1991-05-01 00:00:00

abstract：:The synthesis of 60S ribosomal subunits in Saccharomyces cerevisiae requires Tif6p, the yeast homologue of mammalian eukaryotic translation initiation factor 6 (eIF6). In the present work, we have isolated a protein kinase from rabbit reticulocyte lysates on the basis of its ability to phosphorylate recombinant human ...

journal_title：Molecular and cellular biology

authors： Basu U,Si K,Deng H,Maitra U

更新日期：2003-09-01 00:00:00