The amino-terminal region of Drosophila MSL1 contains basic, glycine-rich, and leucine zipper-like motifs that promote X chromosome binding, self-association, and MSL2 binding, respectively.


:In Drosophila melanogaster, X chromosome dosage compensation is achieved by doubling the transcription of most X-linked genes. The male-specific lethal (MSL) complex is required for this process and binds to hundreds of sites on the male X chromosome. The MSL1 protein is essential for X chromosome binding and serves as a central scaffold for MSL complex assembly. We find that the amino-terminal region of MSL1 binds to hundreds of sites on the X chromosome in normal males but only to approximately 30 high-affinity sites in the absence of endogenous MSL1. Binding to the high-affinity sites requires a basic motif at the amino terminus that is conserved among Drosophila species. X chromosome binding also requires a conserved leucine zipper-like motif that binds to MSL2. A glycine-rich motif between the basic and leucine-zipper-like motifs mediates MSL1 self-association in vitro and binding of the amino-terminal region of MSL1 to the MSL complex assembled on the male X chromosome. We propose that the basic region may mediate DNA binding and that the glycine-rich region may promote the association of MSL complexes to closely adjacent sites on the X chromosome.


Mol Cell Biol


Li F,Parry DA,Scott MJ




Has Abstract


2005-10-01 00:00:00














  • Distinctive features of Drosophila alternative splicing factor RS domain: implication for specific phosphorylation, shuttling, and splicing activation.

    abstract::The human splicing factor 2, also called human alternative splicing factor (hASF), is the prototype of the highly conserved SR protein family involved in constitutive and regulated splicing of metazoan mRNA precursors. Here we report that the Drosophila homologue of hASF (dASF) lacks eight repeating arginine-serine di...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Allemand E,Gattoni R,Bourbon HM,Stevenin J,Cáceres JF,Soret J,Tazi J

    更新日期:2001-02-01 00:00:00

  • GATA1-mediated megakaryocyte differentiation and growth control can be uncoupled and mapped to different domains in GATA1.

    abstract::The DNA-binding hemopoietic zinc finger transcription factor GATA1 promotes terminal megakaryocyte differentiation and restrains abnormal immature megakaryocyte expansion. How GATA1 coordinates these fundamental processes is unclear. Previous studies of synthetic and naturally occurring mutant GATA1 molecules demonstr...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Kuhl C,Atzberger A,Iborra F,Nieswandt B,Porcher C,Vyas P

    更新日期:2005-10-01 00:00:00

  • An intronic locus control region plays an essential role in the establishment of an autonomous hepatic chromatin domain for the human vitamin D-binding protein gene.

    abstract::The human vitamin D-binding protein (hDBP) gene exists in a cluster of four liver-expressed genes. A minimal hDBP transgene, containing a defined set of liver-specific DNase I hypersensitive sites (HSs), is robustly expressed in mouse liver in a copy-number-dependent manner. Here we evaluate these HSs for function. De...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Hiroki T,Liebhaber SA,Cooke NE

    更新日期:2007-11-01 00:00:00

  • rRNA suppressor of a eukaryotic translation initiation factor 5B/initiation factor 2 mutant reveals a binding site for translational GTPases on the small ribosomal subunit.

    abstract::The translational GTPases promote initiation, elongation, and termination of protein synthesis by interacting with the ribosome. Mutations that impair GTP hydrolysis by eukaryotic translation initiation factor 5B/initiation factor 2 (eIF5B/IF2) impair yeast cell growth due to failure to dissociate from the ribosome fo...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Shin BS,Kim JR,Acker MG,Maher KN,Lorsch JR,Dever TE

    更新日期:2009-02-01 00:00:00

  • Cell cycle regulation of RNA polymerase III transcription.

    abstract::Inactivation of the TATA-binding protein-containing complex TFIIIB contributes to the mitotic repression of RNA polymerase III transcription, both in frogs and in humans (J. M. Gottesfeld, V. J. Wolf, T. Dang, D. J. Forbes, and P. Hartl, Science 263:81-84, 1994; R. J. White, T. M. Gottlieb, C. S. Downes, and S. P. Jac...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: White RJ,Gottlieb TM,Downes CS,Jackson SP

    更新日期:1995-12-01 00:00:00

  • Early embryonic death in mice lacking the beta-catenin-binding protein Duplin.

    abstract::The Wnt signaling pathway plays a pivotal role in vertebrate early development and morphogenesis. Duplin (axis duplication inhibitor) interacts with beta-catenin and prevents its binding to Tcf, thereby inhibiting downstream Wnt signaling. Here we show that Duplin is expressed predominantly from early- to mid-stage mo...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Nishiyama M,Nakayama K,Tsunematsu R,Tsukiyama T,Kikuchi A,Nakayama KI

    更新日期:2004-10-01 00:00:00

  • Cell-specific interaction of retinoic acid receptors with target genes in mouse embryonic fibroblasts and embryonic stem cells.

    abstract::All-trans retinoic acid (RA) induces transforming growth factor beta (TGF-beta)-dependent autocrine growth of mouse embryonic fibroblasts (MEFs). We have used chromatin immunoprecipitation to map 354 RA receptor (RAR) binding loci in MEFs, most of which were similarly occupied by the RAR alpha and RAR gamma receptors....

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Delacroix L,Moutier E,Altobelli G,Legras S,Poch O,Choukrallah MA,Bertin I,Jost B,Davidson I

    更新日期:2010-01-01 00:00:00

  • Phosphorylation of SAF-A/hnRNP-U Serine 59 by Polo-Like Kinase 1 Is Required for Mitosis.

    abstract::Scaffold attachment factor A (SAF-A), also called heterogenous nuclear ribonuclear protein U (hnRNP-U), is phosphorylated on serine 59 by the DNA-dependent protein kinase (DNA-PK) in response to DNA damage. Since SAF-A, DNA-PK catalytic subunit (DNA-PKcs), and protein phosphatase 6 (PP6), which interacts with DNA-PKcs...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Douglas P,Ye R,Morrice N,Britton S,Trinkle-Mulcahy L,Lees-Miller SP

    更新日期:2015-08-01 00:00:00

  • Kinds of mutations formed when a shuttle vector containing adducts of benzo[a]pyrene-7,8-diol-9,10-epoxide replicates in COS7 cells.

    abstract::We have investigated the kinds of mutations induced when a shuttle vector containing covalently bound residues of the (+/-)-7 beta,8 alpha-dihydroxy-9 alpha,10 alpha-epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene (BPDE) replicates in the monkey kidney cell line COS7. The target for detecting mutations was the 200-base pair g...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Yang JL,Maher VM,McCormick JJ

    更新日期:1987-03-01 00:00:00

  • PSF is a novel corepressor that mediates its effect through Sin3A and the DNA binding domain of nuclear hormone receptors.

    abstract::Members of the type II nuclear hormone receptor subfamily (e.g., thyroid hormone receptors [TRs], retinoic acid receptors, retinoid X receptors [RXRs], vitamin D receptor, and the peroxisome proliferator-activated receptors) bind to their response sequences with or without ligand. In the absence of ligand, these DNA-b...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Mathur M,Tucker PW,Samuels HH

    更新日期:2001-04-01 00:00:00

  • Inheritance of extrachromosomal rDNA in Physarum polycephalum.

    abstract::In the acellular slime mold Physarum polycephalum, the several hundred genes coding for rRNA are located on linear extrachromosomal DNA molecules of a discrete size, 60 kilobases. Each molecule contains two genes that are arranged in a palindromic fashion and separated by a central spacer region. We investigated how r...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Ferris PJ,Vogt VM,Truitt CL

    更新日期:1983-04-01 00:00:00

  • V-myc- and c-myc-encoded proteins are associated with the nuclear matrix.

    abstract::A series of extraction procedures were applied to avian nuclei which allowed us to define three types of association of v-myc- and c-myc-encoded proteins with nuclei: (i) a major fraction (60 to 90%) which is retained in DNA- and RNA-depleted nuclei after low- and high-salt extraction, (ii) a small fraction (1%) relea...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Eisenman RN,Tachibana CY,Abrams HD,Hann SR

    更新日期:1985-01-01 00:00:00

  • Two conserved amino acid motifs mediate protein targeting to the micronemes of the apicomplexan parasite Toxoplasma gondii.

    abstract::The micronemal protein 2 (MIC2) of Toxoplasma gondii shares sequence and structural similarities with a series of adhesive molecules of different apicomplexan parasites. These molecules accumulate, through a yet unknown mechanism, in secretory vesicles (micronemes), which together with tubular and membrane structures ...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Di Cristina M,Spaccapelo R,Soldati D,Bistoni F,Crisanti A

    更新日期:2000-10-01 00:00:00

  • Interleukin-6-induced STAT3 and AP-1 amplify hepatocyte nuclear factor 1-mediated transactivation of hepatic genes, an adaptive response to liver injury.

    abstract::Following hepatic injury or stress, gluconeogenic and acute-phase response genes are rapidly upregulated to restore metabolic homeostasis and limit tissue damage. Regulation of the liver-restricted insulin-like growth factor binding protein 1 (IGFBP-1) gene is dramatically altered by changes in the metabolic state and...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Leu JI,Crissey MA,Leu JP,Ciliberto G,Taub R

    更新日期:2001-01-01 00:00:00

  • Analysis of TFIIA function In vivo: evidence for a role in TATA-binding protein recruitment and gene-specific activation.

    abstract::Activation of transcription can occur by the facilitated recruitment of TFIID to promoters by gene-specific activators. To investigate the role of TFIIA in TFIID recruitment in vivo, we exploited a class of yeast TATA-binding protein (TBP) mutants that is activation and DNA binding defective. We found that co-overexpr...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Liu Q,Gabriel SE,Roinick KL,Ward RD,Arndt KM

    更新日期:1999-12-01 00:00:00

  • Repression of GCN5 histone acetyltransferase activity via bromodomain-mediated binding and phosphorylation by the Ku-DNA-dependent protein kinase complex.

    abstract::GCN5, a putative transcriptional adapter in humans and yeast, possesses histone acetyltransferase (HAT) activity which has been linked to GCN5's role in transcriptional activation in yeast. In this report, we demonstrate a functional interaction between human GCN5 (hGCN5) and the DNA-dependent protein kinase (DNA-PK) ...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Barlev NA,Poltoratsky V,Owen-Hughes T,Ying C,Liu L,Workman JL,Berger SL

    更新日期:1998-03-01 00:00:00

  • Plakophilin-2 promotes tumor development by enhancing ligand-dependent and -independent epidermal growth factor receptor dimerization and activation.

    abstract::Epidermal growth factor (EGF) receptor (EGFR) has been implicated in tumor development and invasion. Dimerization and autophosphorylation of EGFR are the critical events for EGFR activation. However, the regulation of EGF-dependent and EGF-independent dimerization and phosphorylation of EGFR has not been fully underst...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Arimoto K,Burkart C,Yan M,Ran D,Weng S,Zhang DE

    更新日期:2014-10-01 00:00:00

  • In vivo mitochondrial p53 translocation triggers a rapid first wave of cell death in response to DNA damage that can precede p53 target gene activation.

    abstract::p53 promotes apoptosis in response to death stimuli by transactivation of target genes and by transcription-independent mechanisms. We recently showed that wild-type p53 rapidly translocates to mitochondria in response to multiple death stimuli in cultured cells. Mitochondrial p53 physically interacts with antiapoptot...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Erster S,Mihara M,Kim RH,Petrenko O,Moll UM

    更新日期:2004-08-01 00:00:00

  • RotundRacGAP functions with Ras during spermatogenesis and retinal differentiation in Drosophila melanogaster.

    abstract::Our analysis of rotund (rn) null mutations in Drosophila melanogaster revealed that deletion of the rn locus affects both spermatid and retinal differentiation. In the male reproductive system, the absence of RnRacGAP induced small testes, empty seminal vesicles, short testicular cysts, reduced amounts of interspermat...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Bergeret E,Pignot-Paintrand I,Guichard A,Raymond K,Fauvarque MO,Cazemajor M,Griffin-Shea R

    更新日期:2001-09-01 00:00:00

  • Neutral sphingomyelinase 1 deficiency in the mouse causes no lipid storage disease.

    abstract::Sphingomyelin is a major lipid in the bilayer of subcellular membranes of eukaryotic cells. Different sphingomyelinases catalyze the initial step in the catabolism of sphingomyelin, the hydrolysis to phosphocholine and ceramide. Sphingomyelinases have been postulated to generate ceramide as a lipophilic second messeng...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Zumbansen M,Stoffel W

    更新日期:2002-06-01 00:00:00

  • TIN2 Functions with TPP1/POT1 To Stimulate Telomerase Processivity.

    abstract::TIN2 is an important regulator of telomere length, and mutations in TINF2, the gene encoding TIN2, cause short-telomere syndromes. While the genetics underscore the importance of TIN2, the mechanism through which TIN2 regulates telomere length remains unclear. Here, we tested the effects of human TIN2 on telomerase ac...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Pike AM,Strong MA,Ouyang JPT,Greider CW

    更新日期:2019-10-11 00:00:00

  • Serum response factor is required for immediate-early gene activation yet is dispensable for proliferation of embryonic stem cells.

    abstract::Addition of serum to mitogen-starved cells activates the cellular immediate-early gene (IEG) response. Serum response factor (SRF) contributes to such mitogen-stimulated transcriptional induction of many IEGs during the G0-G1 cell cycle transition. SRF is also believed to be essential for cell cycle progression, as im...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Schratt G,Weinhold B,Lundberg AS,Schuck S,Berger J,Schwarz H,Weinberg RA,Rüther U,Nordheim A

    更新日期:2001-04-01 00:00:00

  • Rapamycin antifungal action is mediated via conserved complexes with FKBP12 and TOR kinase homologs in Cryptococcus neoformans.

    abstract::Cryptococcus neoformans is a fungal pathogen that causes meningitis in patients immunocompromised by AIDS, chemotherapy, organ transplantation, or high-dose steroids. Current antifungal drug therapies are limited and suffer from toxic side effects and drug resistance. Here, we defined the targets and mechanisms of ant...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Cruz MC,Cavallo LM,Görlach JM,Cox G,Perfect JR,Cardenas ME,Heitman J

    更新日期:1999-06-01 00:00:00

  • Fibroblast growth factor and transforming growth factor beta repress transcription of the myogenic regulatory gene MyoD1.

    abstract::In this report, we demonstrate that myogenic cultures inhibited from differentiating by treatment with fibroblast growth factor or transforming growth factor beta show reduced levels of MyoD1 mRNA. Although this repression may contribute to the inhibition of myogenesis by growth factors, additional regulatory pathways...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Vaidya TB,Rhodes SJ,Taparowsky EJ,Konieczny SF

    更新日期:1989-08-01 00:00:00

  • Proteasome-mediated degradation of cotranslationally damaged proteins involves translation elongation factor 1A.

    abstract::Rad23 and Rpn10 play synergistic roles in the recognition of ubiquitinated proteins by the proteasome, and loss of both proteins causes growth and proteolytic defects. However, the physiological targets of Rad23 and Rpn10 have not been well defined. We report that rad23Delta rpn10Delta is unable to grow in the presenc...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Chuang SM,Chen L,Lambertson D,Anand M,Kinzy TG,Madura K

    更新日期:2005-01-01 00:00:00

  • Calreticulin interacts with C/EBPalpha and C/EBPbeta mRNAs and represses translation of C/EBP proteins.

    abstract::We previously identified an RNA binding protein, CUGBP1, which binds to GCN repeats located within the 5' region of C/EBPbeta mRNAs and regulates translation of C/EBPbeta isoforms. To further investigate the role of RNA binding proteins in the posttranscriptional control of C/EBP proteins, we purified additional RNA b...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Timchenko LT,Iakova P,Welm AL,Cai ZJ,Timchenko NA

    更新日期:2002-10-01 00:00:00

  • The ternary complex factor Net regulates cell migration through inhibition of PAI-1 expression.

    abstract::Net, Elk-1, and Sap-1 are members of the ternary complex factor (TCF) subfamily of Ets transcription factors. They form ternary complexes with serum response factor (SRF) on serum response elements of immediate early genes such as c-fos and egr-1 and mediate responses to growth factors and mitogen-activated protein ki...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Buchwalter G,Gross C,Wasylyk B

    更新日期:2005-12-01 00:00:00

  • The Drosophila polycomb protein interacts with nucleosomal core particles In vitro via its repression domain.

    abstract::The proteins of the Polycomb group (PcG) are required for maintaining regulator genes, such as the homeotic selectors, stably and heritably repressed in appropriate developmental domains. It has been suggested that PcG proteins silence genes by creating higher-order chromatin structures at their chromosomal targets, t...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Breiling A,Bonte E,Ferrari S,Becker PB,Paro R

    更新日期:1999-12-01 00:00:00

  • Nonsense-containing mRNAs that accumulate in the absence of a functional nonsense-mediated mRNA decay pathway are destabilized rapidly upon its restitution.

    abstract::Nonsense-mediated mRNA decay (NMD) is a conserved proofreading mechanism that protects eukaryotic cells from the potentially deleterious effects of truncated proteins. Studies of Saccharomyces cerevisiae imply that NMD is a predominantly cytoplasmic decay pathway, while studies of mammalian systems suggest that decay ...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Maderazo AB,Belk JP,He F,Jacobson A

    更新日期:2003-02-01 00:00:00

  • Developmental regulation and tissue distribution of the liver transcription factor LFB1 (HNF1) in Xenopus laevis.

    abstract::The transcription factor LFB1 (HNF1) was initially identified as a regulator of liver-specific gene expression in mammals. It interacts with the promoter element HP1, which is functionally conserved between mammals and amphibians, suggesting that a homologous factor, XLFB1, also exists in Xenopus laevis. To study the ...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Bartkowski S,Zapp D,Weber H,Eberle G,Zoidl C,Senkel S,Klein-Hitpass L,Ryffel GU

    更新日期:1993-01-01 00:00:00