Requirement of PDZ-containing proteins for cell cycle regulation and differentiation in the mouse lens epithelium.

abstract：:Very little is known about the mechanisms mediating longevities of mRNAs. As a means of identifying potential cis- and trans-acting elements which stabilize an individual mRNA, naturally occurring mutations that decreased stability of the normally long-lived globin mRNA were analyzed. Our previous studies demonstrated...

journal_title：Molecular and cellular biology

authors： Weiss IM,Liebhaber SA

更新日期：1995-05-01 00:00:00

abstract：:Heterokaryons of Saccharomyces cerevisiae have been constructed utilizing the kar1-1 mutation, which prevents nuclear fusion during conjugation (J. Conde and G. Fink, Proc. Natl. Acad. Sci. U.S.A. 73:3651-3655, 1976). Each heterokaryon contained two haploid nuclei that were marked on several chromosomes. They segregat...

journal_title：Molecular and cellular biology

authors： Dutcher SK

更新日期：1981-03-01 00:00:00

abstract：:This is the first study reporting the inactivation of a member of the mouse gene family of toxin-related ecto-ADP-ribosyltransferases (ARTs). Transfer of the ADP-ribose moiety from NAD onto extracellular arginine residues on T-cell membrane proteins is mediated by glycosylphosphatidylinositol-linked cell surface ARTs....

journal_title：Molecular and cellular biology

authors： Ohlrogge W,Haag F,Löhler J,Seman M,Littman DR,Killeen N,Koch-Nolte F

更新日期：2002-11-01 00:00:00

abstract：:Determining the roles of Rel/NF-kappaB transcription factors in mouse skin development with loss-of-function mutants has been limited by redundancy among these proteins and by embryonic lethality associated with the absence of RelA. Using mice lacking RelA and c-rel, which survive throughout embryogenesis on a tumor n...

journal_title：Molecular and cellular biology

authors： Gugasyan R,Voss A,Varigos G,Thomas T,Grumont RJ,Kaur P,Grigoriadis G,Gerondakis S

更新日期：2004-07-01 00:00:00

abstract：:One hallmark of tumor formation is the transcriptional upregulation of human telomerase reverse transcriptase, hTERT, and the resultant induction of telomerase activity. However, little is presently understood about how hTERT is differentially activated in tumor cells versus normal somatic cells. Specifically, it is u...

journal_title：Molecular and cellular biology

authors： Goueli BS,Janknecht R

更新日期：2004-01-01 00:00:00

abstract：:Chicken embryo fibroblast cells overexpressing activated mutant forms of human pp60c-src, but not those overexpressing normal human pp60c-src, exhibited high levels of type I phosphatidylinositol (PI) kinase activity associated with pp60c-src. Levels of PI kinase activity were positively correlated with src tyrosine p...

journal_title：Molecular and cellular biology

authors： Chan TO,Tanaka A,Bjorge JD,Fujita DJ

更新日期：1990-06-01 00:00:00

abstract：:SEC63 encodes a protein required for secretory protein translocation into the endoplasmic reticulum (ER) of Saccharomyces cerevisiae (J. A. Rothblatt, R. J. Deshaies, S. L. Sanders, G. Daum, and R. Schekman, J. Cell Biol. 109:2641-2652, 1989). Antibody directed against a recombinant form of the protein detects a 73-kD...

journal_title：Molecular and cellular biology

authors： Feldheim D,Rothblatt J,Schekman R

更新日期：1992-07-01 00:00:00

abstract：:Different point mutations in the nucleolar protein fibrillarin (Nop1p in Saccharomyces cerevisiae) can inhibit different steps in ribosome synthesis. A screen for mutations that are synthetically lethal (sl) with the nop1-5 allele, which inhibits pre-rRNA processing, identified NOP56. An independent sl mutation screen...

journal_title：Molecular and cellular biology

authors： Gautier T,Bergès T,Tollervey D,Hurt E

更新日期：1997-12-01 00:00:00

abstract：:The abundance of crystallins (> 80% of the soluble protein) in the ocular lens provides advantageous markers for selective gene expression during cellular differentiation. Here we show by functional and protein-DNA binding experiments that the chicken alpha A-crystallin gene is regulated by at least five control eleme...

journal_title：Molecular and cellular biology

authors： Cvekl A,Sax CM,Bresnick EH,Piatigorsky J

更新日期：1994-11-01 00:00:00

abstract：:In Saccharomyces cerevisiae, a phosphorelay signal transduction pathway composed of Sln1p, Ypd1p, and Ssk1p, which are homologous to bacterial two-component signal transducers, is involved in the osmosensing mechanism. In response to high osmolarity, the phosphorelay system is inactivated and Ssk1p remains unphosphory...

journal_title：Molecular and cellular biology

authors： Sato N,Kawahara H,Toh-e A,Maeda T

更新日期：2003-09-01 00:00:00

abstract：:When herpes simplex virus infects permissive cells, the viral regulatory protein VP16 forms a specific complex with HCF-1, a preexisting nuclear protein involved in cell proliferation. The majority of HCF-1 in the cell is a complex of associated amino (HCF-1(N))- and carboxy (HCF-1(C))-terminal subunits that result fr...

journal_title：Molecular and cellular biology

authors： Wilson AC,Boutros M,Johnson KM,Herr W

更新日期：2000-09-01 00:00:00

abstract：:With the murine retinoblastoma (RB) cDNA, a series of RB mutants were expressed in COS-1 cells and the pRB products were assessed for their ability (i) to bind to large T antigen (large T), (ii) to become modified by phosphorylation, and (iii) to localize in the nucleus. All point mutations and deletions introduced in...

journal_title：Molecular and cellular biology

authors： Hamel PA,Cohen BL,Sorce LM,Gallie BL,Phillips RA

更新日期：1990-12-01 00:00:00

abstract：:The transcriptional coactivator Cbp plays an important role in a wide range of cellular processes, including proliferation, differentiation, and apoptosis. Although studies have shown its requirement for hematopoietic stem cell (HSC) development, its role in adult HSC maintenance, as well as the cellular and molecular...

journal_title：Molecular and cellular biology

authors： Chan WI,Hannah RL,Dawson MA,Pridans C,Foster D,Joshi A,Göttgens B,Van Deursen JM,Huntly BJ

更新日期：2011-12-01 00:00:00

abstract：:Telomere stabilization is critical for tumorigenesis. A number of tumors and cell lines use a recombination-based mechanism, alternative lengthening of telomeres (ALT), to maintain telomere repeat arrays. Current data suggest that the mutation of p53 facilitates the activation of this pathway. In addition to its funct...

journal_title：Molecular and cellular biology

authors： Razak ZR,Varkonyi RJ,Kulp-McEliece M,Caslini C,Testa JR,Murphy ME,Broccoli D

更新日期：2004-07-01 00:00:00

abstract：:The alpha-myosin heavy-chain (alpha-MHC) gene is the major structural protein in the adult rodent myocardium. Its expression is restricted to the heart by a complex interplay of trans-acting factors and their cis-acting sites. However, to date, the factors that have been shown to regulate expression of this gene have ...

journal_title：Molecular and cellular biology

authors： Molkentin JD,Kalvakolanu DV,Markham BE

更新日期：1994-07-01 00:00:00

abstract：:Dyskerin binds the H/ACA box of human telomerase RNA and is a core telomerase subunit required for RNP biogenesis and enzyme function in vivo. Missense mutations in dyskerin result in dyskeratosis congenita, a complex syndrome characterized by bone marrow failure, telomerase enzyme deficiency, and progressive telomere...

journal_title：Molecular and cellular biology

authors： Kannan K,Nelson AD,Shippen DE

更新日期：2008-04-01 00:00:00

abstract：:We analyzed the transcriptional properties of a repetitive sequence element, BmX, that belongs to a large gene family (approximately 2 x 10(4) copies) in the genome of the Bombyx mori silkworm. We discovered BmX elements because of their ability to direct transcription by polymerase III in vitro and used them to test ...

journal_title：Molecular and cellular biology

authors： Wilson ET,Condliffe DP,Sprague KU

更新日期：1988-02-01 00:00:00

abstract：:The dorsal protein (DL) regulates the transcriptional activity of several genes that determine cell fate along the dorsoventral axis of the Drosophila melanogaster embryo. DL is present at high levels in ventral nuclei, where it activates some genes (twi and sna) and represses others (zen, dpp, and tld). DL shows homo...

journal_title：Molecular and cellular biology

authors： Kirov N,Childs S,O'Connor M,Rushlow C

更新日期：1994-01-01 00:00:00

abstract：:FUD6, a nonphotosynthetic mutant of Chlamydomonas reinhardtii, was previously found to be deficient in the synthesis of subunit IV of the cytochrome b6/f complex, the chloroplast petD gene product (C. Lemaire, J. Girard-Bascou, F.-A. Wollman, and P. Bennoun, Biochim. Biophys. Acta 851:229-238, 1986). The lesion in FUD...

journal_title：Molecular and cellular biology

authors： Sturm NR,Kuras R,Büschlen S,Sakamoto W,Kindle KL,Stern DB,Wollman FA

更新日期：1994-09-01 00:00:00

abstract：:Transthyretin (TTR) and alpha 1-antitrypsin (alpha 1-AT) are expressed at high levels in the liver and also in at least one other cell type. We report here a detailed analysis of the proximal regulatory region of the TTR gene, which has uncovered two new DNA-binding factors that are present mainly (or only) in hepatoc...

journal_title：Molecular and cellular biology

authors： Costa RH,Grayson DR,Darnell JE Jr

更新日期：1989-04-01 00:00:00

abstract：:The human immunodeficiency virus type 1 Rev protein contains a nuclear export signal (NES) that is required for Rev-mediated RNA export in mammals as well as in the yeast Saccharomyces cerevisiae. The Rev NES has been shown to specifically interact with a human (hRIP/RAB1) and a yeast (yRip1p) protein in the two-hybri...

journal_title：Molecular and cellular biology

authors： Stutz F,Izaurralde E,Mattaj IW,Rosbash M

更新日期：1996-12-01 00:00:00

abstract：:RNR2 encodes the small subunit of ribonucleotide reductase, the enzyme that catalyzes the first step in the pathway for the production of deoxyribonucleotides needed for DNA synthesis. RNR2 is a member of a group of genes whose activities are cell cycle regulated and that are transcriptionally induced in response to t...

journal_title：Molecular and cellular biology

authors： Elledge SJ,Davis RW

更新日期：1989-11-01 00:00:00

abstract：:We have characterized the putative AP1 site in the backbone of pUC plasmids and found unique regulatory effects. The site, which mapped to a 19-bp region around nucleotide 37, conferred transcriptional activation by Jun or Jun/Fos that was boosted up to fivefold by unliganded thyroid hormone receptor (TR). Thyroid hor...

journal_title：Molecular and cellular biology

authors： Lopez G,Schaufele F,Webb P,Holloway JM,Baxter JD,Kushner PJ

更新日期：1993-05-01 00:00:00

abstract：:Over 400 Krüppel-associated box zinc finger proteins (KRAB-ZFPs) are encoded in mammalian genomes. While KRAB-ZFPs strongly repress transcription in vitro, little is known about their biological function or gene targets in vivo. Regulator of sex limitation 1 (Rsl1), one of the first KRAB-Zfp genes assigned a physiolog...

journal_title：Molecular and cellular biology

authors： Krebs CJ,Schultz DC,Robins DM

更新日期：2012-09-01 00:00:00

abstract：:We have developed an in vitro system which faithfully reproduces several aspects of general mRNA stability. Poly(A)- RNAs were rapidly and efficiently degraded in this system with no detectable intermediates by a highly processive 3'-to-5' exonuclease activity. The addition of a poly(A) tail of at least 30 bases, or a...

journal_title：Molecular and cellular biology

authors： Ford LP,Bagga PS,Wilusz J

更新日期：1997-01-01 00:00:00

abstract：:TGF-alpha-PE40 is a hybrid protein composed of transforming growth factor-alpha (TGF-alpha) fused to a 40,000-dalton segment of Pseudomonas exotoxin A (PE40). This hybrid protein possesses the receptor-binding activity of TGF-alpha and the cell-killing properties of PE40. These properties enable TGF-alpha-PE40 to bind...

journal_title：Molecular and cellular biology

authors： Edwards GM,DeFeo-Jones D,Tai JY,Vuocolo GA,Patrick DR,Heimbrook DC,Oliff A

更新日期：1989-07-01 00:00:00

abstract：:Eukaryotic viruses have devised numerous strategies to downregulate activity of the interferon-induced, double-stranded (dsRNA)-activated protein kinase (referred to as p68 on the basis of its Mr of 68,000 in human cells). Viruses must exert this control to avoid extensive phosphorylation of the alpha subunit of eukar...

journal_title：Molecular and cellular biology

authors： Katze MG,Wambach M,Wong ML,Garfinkel M,Meurs E,Chong K,Williams BR,Hovanessian AG,Barber GN

更新日期：1991-11-01 00:00:00

abstract：:We previously identified an RNA binding protein, CUGBP1, which binds to GCN repeats located within the 5' region of C/EBPbeta mRNAs and regulates translation of C/EBPbeta isoforms. To further investigate the role of RNA binding proteins in the posttranscriptional control of C/EBP proteins, we purified additional RNA b...

journal_title：Molecular and cellular biology

authors： Timchenko LT,Iakova P,Welm AL,Cai ZJ,Timchenko NA

更新日期：2002-10-01 00:00:00

abstract：:In the accompanying report (C. F. Webb, C. Das, S. Eaton, K. Calame, and P. Tucker, Mol. Cell. Biol. 11:5197-5205, 1991), we characterize B-cell-specific protein-DNA interactions at -500 and -200 bp upstream of the mu immunoglobulin heavy chain promoter whose abundances were increased by interleukin-5 plus antigen. Be...

journal_title：Molecular and cellular biology

authors： Webb CF,Das C,Eneff KL,Tucker PW

更新日期：1991-10-01 00:00:00

abstract：:Although RNA and RNA-binding proteins have been linked to double-strand breaks (DSBs), little is known regarding their roles in the cellular response to DSBs and, if any, in the repair process. Here, we provide direct evidence for the presence of RNA-DNA hybrids at DSBs and suggest that binding of RNA to DNA at DSBs m...

journal_title：Molecular and cellular biology

authors： Li L,Germain DR,Poon HY,Hildebrandt MR,Monckton EA,McDonald D,Hendzel MJ,Godbout R

更新日期：2016-10-28 00:00:00