The signal response of IkappaB alpha is regulated by transferable N- and C-terminal domains.

abstract：:Poliovirus polysomal RNA is naturally uncapped, and as such, its translation must bypass any 5' cap-dependent ribosome recognition event. To elucidate the manner by which poliovirus mRNA is translated, we have determined the translational efficiencies of a series of deletion mutants within the 5' noncoding region of t...

journal_title：Molecular and cellular biology

authors： Pelletier J,Kaplan G,Racaniello VR,Sonenberg N

更新日期：1988-03-01 00:00:00

abstract：:Alkylpurine-DNA-N-glycosylase (APNG) null mice have been generated by homologous recombination in embryonic stem cells. The null status of the animals was confirmed at the mRNA level by reverse transcription-PCR and by the inability of cell extracts of tissues from the knockout (ko) animals to release 3-methyladenine ...

journal_title：Molecular and cellular biology

authors： Elder RH,Jansen JG,Weeks RJ,Willington MA,Deans B,Watson AJ,Mynett KJ,Bailey JA,Cooper DP,Rafferty JA,Heeran MC,Wijnhoven SW,van Zeeland AA,Margison GP

更新日期：1998-10-01 00:00:00

abstract：:A potential p120 GTPase-activating protein (RasGAP) effector, G3BP (RasGAP Src homology 3 [SH3] binding protein), was previously identified based on its ability to bind the SH3 domain of RasGAP. Here we show that G3BP colocalizes and physically interacts with RasGAP at the plasma membrane of serum-stimulated but not q...

journal_title：Molecular and cellular biology

authors： Gallouzi IE,Parker F,Chebli K,Maurier F,Labourier E,Barlat I,Capony JP,Tocque B,Tazi J

更新日期：1998-07-01 00:00:00

abstract：:Anaplastic lymphoma kinase (ALK) is a receptor tyrosine kinase, initially discovered as part of the NPM-ALK fusion protein, resulting from the t(2;5) translocation that is frequently associated with anaplastic large-cell lymphomas. The native ALK protein is normally expressed in the developing and, at a weaker level, ...

journal_title：Molecular and cellular biology

authors： Mourali J,Bénard A,Lourenço FC,Monnet C,Greenland C,Moog-Lutz C,Racaud-Sultan C,Gonzalez-Dunia D,Vigny M,Mehlen P,Delsol G,Allouche M

更新日期：2006-08-01 00:00:00

abstract：:Retinoblastoma (RB) tumors develop when both alleles of a gene (RB1) are mutated and unable to function normally. Recently, Friend et al. [S. H. Friend, R. Bernards, S. Rogelj, R. A. Weinberg, J. M. Rapaport, D. M. Albert, and T. P. Dryja, Nature (London) 32:643-646, 1986] reported the cloning of a gene, 4.7R, with so...

journal_title：Molecular and cellular biology

authors： Goddard AD,Balakier H,Canton M,Dunn J,Squire J,Reyes E,Becker A,Phillips RA,Gallie BL

更新日期：1988-05-01 00:00:00

abstract：:The family of p21-activated protein kinases (PAKs) appear to be present in all organisms that have Cdc42-like GTPases. In mammalian cells, PAKs have been implicated in the activation of mitogen-activated protein kinase cascades, but there are no reported effects of these kinases on the cytoskeleton. Recently we have s...

journal_title：Molecular and cellular biology

authors： Manser E,Huang HY,Loo TH,Chen XQ,Dong JM,Leung T,Lim L

更新日期：1997-03-01 00:00:00

abstract：:Studies were undertaken to determine the effect of the Ras suppressor Rsu-1 on Ras signal transduction pathways in two different cell backgrounds. An expression vector containing the mouse rsu-1 cDNA under the control of a mouse mammary tumor virus promoter was introduced into NIH 3T3 cells and the pheochromocytoma ce...

journal_title：Molecular and cellular biology

authors： Masuelli L,Cutler ML

更新日期：1996-10-01 00:00:00

abstract：:Genomic DNA in higher eucaryotic cells is organized into a series of loops, each of which may be affixed at its base to the nuclear matrix via a specific matrix attachment region (MAR). In this report, we describe the distribution of MARs within the amplified dihydrofolate reductase (DHFR) domain (amplicon) in the met...

journal_title：Molecular and cellular biology

authors： Dijkwel PA,Hamlin JL

更新日期：1988-12-01 00:00:00

abstract：:The protein kinase inhibitor 2-aminopurine (2-AP) greatly stimulated expression in human promonocytes-macrophages of plasmid constructs carrying various reporter genes (chloramphenicol acetyltransferase, lacZ, firefly luciferase [luc], and Salmonella typhimurium histidinol dehydrogenase [his]) driven by the human immu...

journal_title：Molecular and cellular biology

authors： Brown FL,Tahaoglu E,Graham GJ,Maio JJ

更新日期：1993-09-01 00:00:00

abstract：:Fibroblast growth factor 1 (FGF-1) is a powerful angiogenic factor whose gene structure contains four promoters, giving rise to a process of alternative splicing resulting in four mRNAs with alternative 5' untranslated regions (5' UTRs). Here we have identified, by using double luciferase bicistronic vectors, the pres...

journal_title：Molecular and cellular biology

authors： Martineau Y,Le Bec C,Monbrun L,Allo V,Chiu IM,Danos O,Moine H,Prats H,Prats AC

更新日期：2004-09-01 00:00:00

abstract：:We have previously described a transcription unit on human chromosome 8, designated as PVT, that is consistently disrupted by the minority forms of translocations [t(2;8) and t(8;22)] in Burkitt's lymphoma. PVT begins 57 kilobase pairs downstream of the proto-oncogene MYC and is more than 200 kilobase pairs in length....

journal_title：Molecular and cellular biology

authors： Shtivelman E,Bishop JM

更新日期：1990-04-01 00:00:00

abstract：:We describe an interaction between homeodomain-interacting protein kinase 1 (HIPK1) and Daxx, two transcriptional regulators important in transducing growth-regulatory signals. We demonstrate that HIPK1 is ubiquitously expressed in mice and humans and localizes predominantly to the nucleus. Daxx normally resides withi...

journal_title：Molecular and cellular biology

authors： Ecsedy JA,Michaelson JS,Leder P

更新日期：2003-02-01 00:00:00

abstract：:Platelet-derived growth factor alpha receptor (PDGF alpha R) is a transmembrane tyrosine kinase receptor for all three existing PDGF isoforms, AA, AB, and BB. Transcripts of PDGF alpha R are detected as early as in fertilized mouse eggs and throughout adulthood in a time- and space-specific manner, thereby suggesting ...

journal_title：Molecular and cellular biology

authors： Wang C,Song B

更新日期：1996-02-01 00:00:00

abstract：:By injecting the appropriately constructed plasmids into one-cell Xenopus embryos, we determined that the 3' region of the maternal Xenopus Eg2 mRNA confers instability on the chimeric mRNA transcribed from these plasmids. This instability, like that of the maternal Eg2 transcript, was abolished by treatment of the em...

journal_title：Molecular and cellular biology

authors： Bouvet P,Paris J,Phillippe M,Osborne HB

更新日期：1991-06-01 00:00:00

abstract：:The level of phosphotyrosine in vinculin was determined in chicken embryo fibroblasts transformed by various strains of avian sarcoma virus. As previously reported (Sefton et al., Cell 24:165-174, 1981), vinculin was phosphorylated at tyrosine residues in most cultures examined, but the level varied greatly and no det...

journal_title：Molecular and cellular biology

authors： Antler AM,Greenberg ME,Edelman GM,Hanafusa H

更新日期：1985-01-01 00:00:00

abstract：:The ribosomal proteins (RPs) of Saccharomyces cerevisiae are encoded by 137 genes that are among the most transcriptionally active in the genome. These genes are coordinately regulated: a shift up in temperature leads to a rapid, but temporary, decline in RP mRNA levels. A defect in any part of the secretory pathway l...

journal_title：Molecular and cellular biology

authors： Li B,Nierras CR,Warner JR

更新日期：1999-08-01 00:00:00

abstract：:The structural maintenance of chromosomes (Smc) family members Smc5 and Smc6 are both essential in budding and fission yeasts. Yeast smc5/6 mutants are hypersensitive to DNA damage, and Smc5/6 is recruited to HO-induced double-strand breaks (DSBs), facilitating intersister chromatid recombinational repair. To determin...

journal_title：Molecular and cellular biology

authors： Stephan AK,Kliszczak M,Dodson H,Cooley C,Morrison CG

更新日期：2011-04-01 00:00:00

abstract：:In eukaryotic cells, cohesion between sister chromatids allows chromosomes to biorient on the metaphase plate and holds them together until they separate into daughter cells during mitosis. Cohesion is mediated by the cohesin protein complex. Although the association of this complex with particular regions of the geno...

journal_title：Molecular and cellular biology

authors： Bausch C,Noone S,Henry JM,Gaudenz K,Sanderson B,Seidel C,Gerton JL

更新日期：2007-12-01 00:00:00

abstract：:Axin is a recently identified protein encoded by the fused locus in mice that is required for normal vertebrate axis formation. We have defined a 25-amino-acid sequence in axin that comprises the glycogen synthase kinase 3beta (GSK-3beta) interaction domain (GID). In contrast to full-length axin, which has been shown ...

journal_title：Molecular and cellular biology

authors： Hedgepeth CM,Deardorff MA,Rankin K,Klein PS

更新日期：1999-10-01 00:00:00

abstract：:macroH2A histone variants have been implicated to function in gene silencing by several studies, including ones showing a preferential association of macroH2A on the inactive X chromosome. To examine macroH2A function in vivo, we knocked out macroH2A1. macroH2A1 knockout mice are viable and fertile. A broad screen of ...

journal_title：Molecular and cellular biology

authors： Changolkar LN,Costanzi C,Leu NA,Chen D,McLaughlin KJ,Pehrson JR

更新日期：2007-04-01 00:00:00

abstract：:Appropriate cell cycle checkpoint control is essential for the maintenance of cell and organismal homeostasis. Members of the Nek (NIMA-related kinase) family of serine/threonine protein kinases have been implicated in the regulation of various aspects of the cell cycle. We explored the cellular functions of Nek10, a ...

journal_title：Molecular and cellular biology

authors： Moniz LS,Stambolic V

更新日期：2011-01-01 00:00:00

abstract：:The expression of three different actin genes in the sea urchin, Strongylocentrotus purpuratus, was monitored in embryos and adult tissues by using untranslated mRNA sequences as specific hybridization probes. Three distinct patterns of expression were found: muscle specific, embryo specific, and constitutive (i.e., p...

journal_title：Molecular and cellular biology

authors： Garcia R,Paz-Aliaga B,Ernst SG,Crain WR Jr

更新日期：1984-05-01 00:00:00

abstract：:The role of the target cell in its own death mediated by cytotoxic T lymphocytes (CTL) has been controversial. The ability of the pore-forming granule components of CTL to induce target cell death directly has been taken to suggest an essentially passive role for the target. This view of CTL-mediated killing ascribes ...

journal_title：Molecular and cellular biology

authors： Ucker DS,Wilson JD,Hebshi LD

更新日期：1994-01-01 00:00:00

abstract：:SCF complexes are E3 ubiquitin-protein ligases that mediate degradation of regulatory and signaling proteins and control G1/S cell cycle progression by degradation of G1 cyclins and the cyclin-dependent kinase inhibitor, Sic1. Interchangeable F-box proteins bind the core SCF components; each recruits a specific subset...

journal_title：Molecular and cellular biology

authors： Ivantsiv Y,Kaplun L,Tzirkin-Goldin R,Shabek N,Raveh D

更新日期：2006-03-01 00:00:00

abstract：:Scaffold proteins have been established as important mediators of signal transduction specificity. The insulin receptor substrate (IRS) proteins represent a critical group of scaffold proteins that are required for signal transduction by the insulin receptor, including the activation of phosphatidylinositol 3 kinase. ...

journal_title：Molecular and cellular biology

authors： Standen CL,Kennedy NJ,Flavell RA,Davis RJ

更新日期：2009-09-01 00:00:00

abstract：:During Xenopus development, the synthesis of ribosomal proteins is regulated at the translational level. To identify the region of the ribosomal protein mRNAs responsible for their typical translational behavior, we constructed a fused gene in which the upstream sequences (promoter) and the 5' untranslated sequence (f...

journal_title：Molecular and cellular biology

authors： Mariottini P,Amaldi F

更新日期：1990-02-01 00:00:00

abstract：:Up to 70% of human genes are associated with regions of nonmethylated DNA called CpG islands (S. Saxonov, P. Berg, and D. L. Brutlag, Proc. Natl. Acad. Sci. U. S. A. 103:1412-1417, 2006). Usually associated with the 5' end of genes, CpG islands are thought to impact gene expression. We previously demonstrated that the...

journal_title：Molecular and cellular biology

authors： Zhou JC,Blackledge NP,Farcas AM,Klose RJ

更新日期：2012-01-01 00:00:00

abstract：:Although phosphoinositide 3-kinase (PI 3-kinase) is essential for cell cycle progression, the molecular mechanisms that regulate its diverse biological effects are poorly understood. We demonstrate here that Rb, a key regulator of cell cycle progression, associates with p55 kDa (p55alpha and p55gamma) regulatory subun...

journal_title：Molecular and cellular biology

authors： Xia X,Cheng A,Akinmade D,Hamburger AW

更新日期：2003-03-01 00:00:00

abstract：:Sequence blocks within the core region were swapped among RNA polymerase II promoters to explore effects on transcription in vitro. The pair of blocks flanking TATA strongly influenced general transcription, with an additional effect on promoter activation. These flanking elements induced a change in the ratio of acti...

journal_title：Molecular and cellular biology

authors： Wolner BS,Gralla JD

更新日期：2000-05-01 00:00:00

abstract：:Mos is a germ cell-specific serine/threonine protein kinase that activates mitogen-activated protein kinase (MAPK) through MAPK kinase (MKK). In Xenopus oocytes, Mos synthesis is required for progesterone-induced activation of MAPK and maturation promoting factor. Injection of Mos or active MAPK causes mitotic arrest ...

journal_title：Molecular and cellular biology

authors： Chen M,Cooper JA

更新日期：1995-09-01 00:00:00