Increased phosphorylation of tyrosine in vinculin does not occur upon transformation by some avian sarcoma viruses.

abstract：:By immunoblotting with antibodies for phosphotyrosine, we have demonstrated that the hematopoietic growth factors interleukin-2, interleukin-3, interleukin-4, and granulocyte-macrophage colony-stimulating factor stimulate the tyrosine phosphorylation of specific sets of proteins in murine hematopoietic progenitor cell...

journal_title：Molecular and cellular biology

authors： Morla AO,Schreurs J,Miyajima A,Wang JY

更新日期：1988-05-01 00:00:00

abstract：:Placental development initially occurs in a low-oxygen (O2) or hypoxic environment. In this report we show that two hypoxia-inducible factors (HIFs), HIF1alpha and HIF2alpha, are essential for determining murine placental cell fates. HIF is a heterodimer composed of HIFalpha and HIFbeta (ARNT) subunits. Placentas from...

journal_title：Molecular and cellular biology

authors： Cowden Dahl KD,Fryer BH,Mack FA,Compernolle V,Maltepe E,Adelman DM,Carmeliet P,Simon MC

更新日期：2005-12-01 00:00:00

abstract：:Homologous recombination of DNA molecules injected into Xenopus laevis oocyte nuclei is extremely efficient when those molecules are linear and have overlapping homologous ends. It was previously shown that a 5'----3' exonuclease activity in oocytes attacks injected linear DNAs and leaves them with single-stranded 3' ...

journal_title：Molecular and cellular biology

authors： Maryon E,Carroll D

更新日期：1991-06-01 00:00:00

abstract：:We identified Ras guanine-releasing protein 3 (RasGRP3) as a guanine exchange factor expressed in blood vessels via an embryonic stem (ES) cell-based gene trap screen to identify novel vascular genes. RasGRP3 is expressed in embryonic blood vessels, down-regulated in mature adult vessels, and reexpressed in newly form...

journal_title：Molecular and cellular biology

authors： Roberts DM,Anderson AL,Hidaka M,Swetenburg RL,Patterson C,Stanford WL,Bautch VL

更新日期：2004-12-01 00:00:00

abstract：:Self-renewal capacity and pluripotency, which are controlled by the Oct3/4-centered transcriptional regulatory network, are major characteristics of embryonic stem (ES) cells. Nuclear hormone receptor Dax1 is one of the crucial factors in the network. Here, we identified an orphan nuclear receptor, Esrrb (estrogen-rel...

journal_title：Molecular and cellular biology

authors： Uranishi K,Akagi T,Sun C,Koide H,Yokota T

更新日期：2013-05-01 00:00:00

abstract：:Posttranscriptional regulation is important for tumor necrosis factor alpha (TNF-alpha) expression in monocytes and macrophages, and an AU-rich element (ARE) in the 3' untranslated region (UTR) of TNF-alpha mRNA is implicated in control of its translation and mRNA stability. Regulation of mRNA turnover is thought to b...

journal_title：Molecular and cellular biology

authors： Dean JL,Wait R,Mahtani KR,Sully G,Clark AR,Saklatvala J

更新日期：2001-02-01 00:00:00

abstract：:The mammalian ABL1 gene encodes the ubiquitously expressed nonreceptor tyrosine kinase ABL. In response to growth factors, cytokines, cell adhesion, DNA damage, oxidative stress, and other signals, ABL is activated to stimulate cell proliferation or differentiation, survival or death, retraction, or migration. ABL als...

journal_title：Molecular and cellular biology

authors： Wang JY

更新日期：2014-04-01 00:00:00

abstract：:The serum response factor (p67SRF) binds to a palindromic sequence in the c-fos serum response element (SRE). A second protein, p62TCF binds in conjunction with p67SRF to form a ternary complex, and it is through this complex that growth factor-induced transcriptional activation of c-fos is thought to take place. A 90...

journal_title：Molecular and cellular biology

authors： Sharrocks AD,Gille H,Shaw PE

更新日期：1993-01-01 00:00:00

abstract：:The expression of the c-jun proto-oncogene is rapidly induced in response to mitogens acting on a large variety of cell surface receptors. The resulting functional activity of c-Jun proteins appears to be critical for cell proliferation. Recently, we have shown that a large family of G protein-coupled receptors (GPCRs...

journal_title：Molecular and cellular biology

authors： Marinissen MJ,Chiariello M,Pallante M,Gutkind JS

更新日期：1999-06-01 00:00:00

abstract：:Recent studies have implicated the role of the SWI/SNF ATP-dependent chromatin remodeling complex in nuclear excision repair (NER), but the mechanism of its function has remained elusive. Here, we show that the human SWI/SNF component human SNF5 (hSNF5) interacts with UV damage recognition factor XPC and colocalizes w...

journal_title：Molecular and cellular biology

authors： Ray A,Mir SN,Wani G,Zhao Q,Battu A,Zhu Q,Wang QE,Wani AA

更新日期：2009-12-01 00:00:00

abstract：:An RNA editing mechanism modifies apolipoprotein B (apo-B) mRNA in the intestine by converting cytosine at nucleotide (nt) 6666 to uracil. To define the sequence requirements for editing, mutant apo-B RNAs were analyzed for the ability to be edited in vitro by enterocyte extracts. Editing was detected by a sensitive a...

journal_title：Molecular and cellular biology

authors： Driscoll DM,Lakhe-Reddy S,Oleksa LM,Martinez D

更新日期：1993-12-01 00:00:00

abstract：:S-Tubercidinylhomocysteine (STH) is a structural analog of S-adenosylhomocysteine and a potent inhibitor of S-adenosylmethionine-dependent methyltransferase reactions. We investigated the effects of STH on HeLa cell mRNA metabolism. Dual labeling studies reveal that STH dramatically inhibits the methylation of HeLa mR...

journal_title：Molecular and cellular biology

authors： Camper SA,Albers RJ,Coward JK,Rottman FM

更新日期：1984-03-01 00:00:00

abstract：:We have analyzed the gene-targeting frequencies and recombination products generated by a series of vectors which target the hprt locus in embryonic stem cells and found the existence of alternative pathways that depend on the location of the double-strand break within the vector. A double-strand break in the targetin...

journal_title：Molecular and cellular biology

authors： Hasty P,Rivera-Pérez J,Bradley A

更新日期：1992-06-01 00:00:00

abstract：:Six4 is a member of the Six family genes, homologues of Drosophila melanogaster sine oculis. The gene is thought to be involved in neurogenesis, myogenesis, and development of other organs, based on its specific expression in certain neuronal cells of the developing embryo and in adult skeletal muscles. To elucidate t...

journal_title：Molecular and cellular biology

authors： Ozaki H,Watanabe Y,Takahashi K,Kitamura K,Tanaka A,Urase K,Momoi T,Sudo K,Sakagami J,Asano M,Iwakura Y,Kawakami K

更新日期：2001-05-01 00:00:00

abstract：:We assayed phosphatidylinositol (PI) kinase (EC 2.7.1.67) activity in detergent extracts of nontransformed or virus-transformed cells. Nontransformed chicken embryo fibroblasts (CEF) contain PI kinase activity with an apparent specific activity of 20 pmol/min per mg of protein. This activity sedimented as a single pea...

journal_title：Molecular and cellular biology

authors： Sugano S,Hanafusa H

更新日期：1985-09-01 00:00:00

abstract：:3'-end processing of nucleus-encoded mRNAs includes the addition of a poly(A) tail that is important for translation initiation. Since the vast majority of chloroplast mRNAs acquire their 3' termini by processing yet are not polyadenylated, we asked whether 3' end maturation plays a role in chloroplast translation. A ...

journal_title：Molecular and cellular biology

authors： Rott R,Levy H,Drager RG,Stern DB,Schuster G

更新日期：1998-08-01 00:00:00

abstract：:The GTPase-activating protein (GAP) stimulates the GTPase reaction of p21 by 5 orders of magnitude such that the kcat of the reaction is increased to 19 s-1. Mutations of residues in loop L1 (Gly-12 and Gly-13), in loop L2 (Thr-35 and Asp-38), and in loop L4 (Gln-61 and Glu-63) influence the reaction in different ways...

journal_title：Molecular and cellular biology

authors： Gideon P,John J,Frech M,Lautwein A,Clark R,Scheffler JE,Wittinghofer A

更新日期：1992-05-01 00:00:00

abstract：:Previous investigations have defined three upstream activation elements--CCAC, A/T, and TATA sequences--necessary for muscle-specific transcription of the myoglobin gene. In the present study, we demonstrate that these three sequences elements, prepared as synthetic oligonucleotide cassettes, function synergistically ...

journal_title：Molecular and cellular biology

authors： Grayson J,Williams RS,Yu YT,Bassel-Duby R

更新日期：1995-04-01 00:00:00

abstract：:HMGA chromatin proteins, a family of gene regulatory factors found at only low concentrations in normal cells, are almost universally overexpressed in cancer cells. HMGA proteins are located in the nuclei of normal cells except during the late S/G(2) phases of the cell cycle, when HMGA1, one of the members of the fami...

journal_title：Molecular and cellular biology

authors： Mao L,Wertzler KJ,Maloney SC,Wang Z,Magnuson NS,Reeves R

更新日期：2009-10-01 00:00:00

abstract：:RNR2 encodes the small subunit of ribonucleotide reductase, the enzyme that catalyzes the first step in the pathway for the production of deoxyribonucleotides needed for DNA synthesis. RNR2 is a member of a group of genes whose activities are cell cycle regulated and that are transcriptionally induced in response to t...

journal_title：Molecular and cellular biology

authors： Elledge SJ,Davis RW

更新日期：1989-11-01 00:00:00

abstract：:A significant challenge to our understanding of eukaryotic transcriptional regulation is to determine how multiple signal transduction pathways converge on a single promoter to regulate transcription in divergent fashions. To study this, we have investigated the transcriptional regulation of the Schizosaccharomyces po...

journal_title：Molecular and cellular biology

authors： Neely LA,Hoffman CS

更新日期：2000-09-01 00:00:00

abstract：:The neutral/neutral two-dimensional (2-D) gel replicon mapping technique has been used to great advantage to localize and characterize origins of replication. Interestingly, many yeast origins display a composite pattern consisting of both a bubble arc and a single-fork arc. Moreover, in every instance in which neutra...

journal_title：Molecular and cellular biology

authors： Kalejta RF,Hamlin JL

更新日期：1996-09-01 00:00:00

abstract：:Transforming growth factor beta (TGF-beta) is the name of a group of closely related polypeptides characterized by a multiplicity of effects, including regulation of extracellular proteolysis and turnover of the extracellular matrix. Its cellular mechanism of action is largely unknown. TGF-beta 1 is a strong and fast ...

journal_title：Molecular and cellular biology

authors： Riccio A,Pedone PV,Lund LR,Olesen T,Olsen HS,Andreasen PA

更新日期：1992-04-01 00:00:00

abstract：:The six human telomeric proteins TRF1, TRF2, RAP1, TIN2, POT1, and TPP1 can form a complex called the telosome/shelterin, which is required for telomere protection and length control. TPP1 has been shown to regulate both POT1 telomere localization and telosome assembly through its binding to TIN2. It remains to be det...

journal_title：Molecular and cellular biology

authors： Chen LY,Liu D,Songyang Z

更新日期：2007-08-01 00:00:00

abstract：:Eukaryotic chromosomes are organized into domains of activity for both transcription and DNA replication. Transcriptional "border," or "insulator," elements have been implicated in mediating the organization of transcriptional domains. However, the DNA sequence elements which might demarcate domains of DNA replication...

journal_title：Molecular and cellular biology

authors： Lu L,Tower J

更新日期：1997-04-01 00:00:00

abstract：:Following hepatic injury or stress, gluconeogenic and acute-phase response genes are rapidly upregulated to restore metabolic homeostasis and limit tissue damage. Regulation of the liver-restricted insulin-like growth factor binding protein 1 (IGFBP-1) gene is dramatically altered by changes in the metabolic state and...

journal_title：Molecular and cellular biology

authors： Leu JI,Crissey MA,Leu JP,Ciliberto G,Taub R

更新日期：2001-01-01 00:00:00

abstract：:Members of the Spalt gene family encode putative transcription factors characterized by seven to nine C2H2 zinc finger motifs. Four genes have been identified in mice--Spalt1 to Spalt4 (Sall1 to Sall4). Spalt homologues are widely expressed in neural and mesodermal tissues during early embryogenesis. Sall3 is normally...

journal_title：Molecular and cellular biology

authors： Parrish M,Ott T,Lance-Jones C,Schuetz G,Schwaeger-Nickolenko A,Monaghan AP

更新日期：2004-08-01 00:00:00

abstract：:Xrn1p of Saccharomyces cerevisiae is a major cytoplasmic RNA turnover exonuclease which is evolutionarily conserved from yeasts to mammals. Deletion of the XRN1 gene causes pleiotropic phenotypes, which have been interpreted as indirect consequences of the RNA turnover defect. By sequence comparisons, we have identifi...

journal_title：Molecular and cellular biology

authors： Solinger JA,Pascolini D,Heyer WD

更新日期：1999-09-01 00:00:00

abstract：:The chromatin structure of TDH3, one of three genes encoding glyceraldehyde phosphate dehydrogenases in Saccharomyces cerevisiae, was analyzed by nuclease digestion. A large hypersensitive region was found at the TDH3 promoter extending from the RNA initiation site at position -40 to position -560. This hypersensitive...

journal_title：Molecular and cellular biology

authors： Pavlović B,Hörz W

更新日期：1988-12-01 00:00:00

abstract：:Allelic forms of DRG1/AFG2 confer resistance to the drug diazaborine, an inhibitor of ribosome biogenesis in Saccharomyces cerevisiae. Our results show that the AAA-ATPase Drg1 is essential for 60S maturation and associates with 60S precursor particles in the cytoplasm. Functional inactivation of Drg1 leads to an incr...

journal_title：Molecular and cellular biology

authors： Pertschy B,Saveanu C,Zisser G,Lebreton A,Tengg M,Jacquier A,Liebminger E,Nobis B,Kappel L,van der Klei I,Högenauer G,Fromont-Racine M,Bergler H

更新日期：2007-10-01 00:00:00