Hematopoietic growth factors activate the tyrosine phosphorylation of distinct sets of proteins in interleukin-3-dependent murine cell lines.

abstract：:When endoplasmic reticulum (ER) homeostasis is perturbed, an adaptive mechanism is triggered and named the unfolded protein response (UPR). Thus far, three known UPR signaling branches (IRE-1, PERK, and ATF-6) mediate the reestablishment of ER functions but can also lead to apoptosis if ER stress is not alleviated. Ho...

journal_title：Molecular and cellular biology

authors： Caruso ME,Jenna S,Bouchecareilh M,Baillie DL,Boismenu D,Halawani D,Latterich M,Chevet E

更新日期：2008-07-01 00:00:00

abstract：:R2 non-long terminal repeat retrotransposable elements insert at a unique site in the 28S rRNA genes of insects. The protein encoded by the single open reading frame of R2 is capable of conducting the initial steps of its integration in vitro. The protein nicks the noncoding strand of the 28S target DNA (the strand wh...

journal_title：Molecular and cellular biology

authors： Luan DD,Eickbush TH

更新日期：1996-09-01 00:00:00

abstract：:Repeat-induced point mutation (RIP) is a process that efficiently detects DNA duplications prior to meiosis in Neurospora crassa and peppers them with G:C to A:T mutations. Cytosine methylation is typically associated with sequences affected by RIP, and methylated cytosines are not limited to CpG dinucleotides. We gen...

journal_title：Molecular and cellular biology

authors： Singer MJ,Marcotte BA,Selker EU

更新日期：1995-10-01 00:00:00

abstract：:MEK kinase 1 (MEKK1) is a 196-kDa protein that, in response to genotoxic agents, was found to undergo phosphorylation-dependent activation. The expression of kinase-inactive MEKK1 inhibited genotoxin-induced apoptosis. Following activation by genotoxins, MEKK1 was cleaved in a caspase-dependent manner into an active 9...

journal_title：Molecular and cellular biology

authors： Widmann C,Gerwins P,Johnson NL,Jarpe MB,Johnson GL

更新日期：1998-04-01 00:00:00

abstract：:The tumor necrosis factor alpha (TNF-alpha) gene is rapidly activated by lipopolysaccharide (LPS). Here, we show that extracellular signal-regulated kinase (ERK) kinase activity but not calcineurin phosphatase activity is required for LPS-stimulated TNF-alpha gene expression. In LPS-stimulated macrophages, the ERK sub...

journal_title：Molecular and cellular biology

authors： Tsai EY,Falvo JV,Tsytsykova AV,Barczak AK,Reimold AM,Glimcher LH,Fenton MJ,Gordon DC,Dunn IF,Goldfeld AE

更新日期：2000-08-01 00:00:00

abstract：:Phospholipase C-gamma2 (PLC-gamma2) plays an important role in B-cell signaling. Phosphorylation of various tyrosine residues of PLC-gamma2 has been implicated in regulation of its lipase activity. With the use of antibodies specific for each of the putative phosphorylation sites, we have now shown that PLC-gamma2 is ...

journal_title：Molecular and cellular biology

authors： Kim YJ,Sekiya F,Poulin B,Bae YS,Rhee SG

更新日期：2004-11-01 00:00:00

abstract：:IkappaB alpha retains the transcription factor NF-kappaB in the cytoplasm, thus inhibiting its function. Various stimuli inactivate IkappaB alpha by triggering phosphorylation of the N-terminal residues Ser32 and Ser36. Phosphorylation of both serines is demonstrated directly by phosphopeptide mapping utilizing calpai...

journal_title：Molecular and cellular biology

authors： Brown K,Franzoso G,Baldi L,Carlson L,Mills L,Lin YC,Gerstberger S,Siebenlist U

更新日期：1997-06-01 00:00:00

abstract：:The TATA-binding protein (TBP), TFIIA, and TFIIB interact with promoter DNA to form a complex required for transcriptional initiation, and many transcriptional regulators function by either stimulating or inhibiting formation of this complex. We have recently identified TBP mutants that are viable in wild-type cells b...

journal_title：Molecular and cellular biology

authors： Biswas D,Imbalzano AN,Eriksson P,Yu Y,Stillman DJ

更新日期：2004-09-01 00:00:00

abstract：:Posttranslational modifications (PTMs) can alter many fundamental properties of a protein. One or combinations of them have been known to regulate the dynamics of many cellular pathways and consequently regulate all vital processes. Understandably, pathogens have evolved sophisticated strategies to subvert these mecha...

journal_title：Molecular and cellular biology

authors： Verma S,Mohapatra G,Ahmad SM,Rana S,Jain S,Khalsa JK,Srikanth CV

更新日期：2015-09-01 00:00:00

abstract：:Ubiquitin is a multifunctional 76-amino-acid protein which plays critical roles in many aspects of cellular metabolism. In Caenorhabditis elegans, the major source of ubiquitin RNA is the polyubiquitin locus, UbiA. UbiA is transcribed as a polycistronic mRNA which contains 11 tandem repeats of ubiquitin sequence and p...

journal_title：Molecular and cellular biology

authors： Graham RW,Jones D,Candido EP

更新日期：1989-01-01 00:00:00

abstract：:Histone acetylation provides a switch between transcriptionally repressive and permissive chromatin. By regulating the chromatin structure at specific promoters, histone acetyltransferases (HATs) carry out important functions during differentiation and development of higher eukaryotes. HAT complexes are present in org...

journal_title：Molecular and cellular biology

authors： Guelman S,Suganuma T,Florens L,Weake V,Swanson SK,Washburn MP,Abmayr SM,Workman JL

更新日期：2006-10-01 00:00:00

abstract：:B-cell stimulatory factor 2 (BSF-2) is a lymphokine which induces the final maturation of B cells. BSF-2 acts on a variety of cells other than B cells, and moreover, expression of BSF-2 mRNA is detected in interleukin-1 beta-stimulated glioblastoma and astrocytoma cell lines. Here, we studied the function of BSF-2 on ...

journal_title：Molecular and cellular biology

authors： Satoh T,Nakamura S,Taga T,Matsuda T,Hirano T,Kishimoto T,Kaziro Y

更新日期：1988-08-01 00:00:00

abstract：:Hepatitis C virus (HCV) NS5B protein is a membrane-associated phosphoprotein that possesses an RNA-dependent RNA polymerase activity. We recently reported that NS5A protein interacts with TRAF2 and modulates tumor necrosis factor alpha (TNF-alpha)-induced NF-kappaB and Jun N-terminal protein kinase (JNK). Since NS5A a...

journal_title：Molecular and cellular biology

authors： Choi SH,Park KJ,Ahn BY,Jung G,Lai MM,Hwang SB

更新日期：2006-04-01 00:00:00

abstract：:cyt-PTP epsilon is a naturally occurring nonreceptor form of the receptor-type protein tyrosine phosphatase (PTP) epsilon. As such, cyt-PTP epsilon enables analysis of phosphatase regulation in the absence of extracellular domains, which participate in dimerization and inactivation of the receptor-type phosphatases re...

journal_title：Molecular and cellular biology

authors： Toledano-Katchalski H,Tiran Z,Sines T,Shani G,Granot-Attas S,den Hertog J,Elson A

更新日期：2003-08-01 00:00:00

abstract：:Fos and Jun transcription factors are induced during the normal course of the proliferative response of quiescent cells to serum or to growth factors. We have shown that delta FosB, an alternatively spliced form of FosB, is formed as rapidly as FosB in serum-stimulated Rat-1A cells. Although delta FosB lacks the C-ter...

journal_title：Molecular and cellular biology

authors： Nakabeppu Y,Oda S,Sekiguchi M

更新日期：1993-07-01 00:00:00

abstract：:It is well accepted that for transcriptional silencing in budding yeast, the evolutionarily conserved lysine deacetylase Sir2, in concert with its partner proteins Sir3 and Sir4, establishes a chromatin structure that prevents RNA polymerase II (Pol II) transcription. However, the mechanism of repression remains contr...

journal_title：Molecular and cellular biology

authors： Gao L,Gross DS

更新日期：2008-06-01 00:00:00

abstract：:We demonstrated that MIF-1, identified initially as a binding activity that associated with the intron I element of the c-myc gene, consists of two polypeptides, the myc intron-binding peptide (MIBP1) and the major histocompatibility class II promoter-binding protein, RFX1. Using a polyclonal antiserum directed agains...

journal_title：Molecular and cellular biology

authors： Reinhold W,Emens L,Itkes A,Blake M,Ichinose I,Zajac-Kaye M

更新日期：1995-06-01 00:00:00

abstract：:Ligand-induced dimerization and transphosphorylation are thought to be important events by which receptor tyrosine kinases generate cellular signals. We have investigated the ability of signalling-defective, truncated fibroblast growth factor (FGF) receptors (FGFR-1 and FGFR-2) to block the FGF response in cells that ...

journal_title：Molecular and cellular biology

authors： Li Y,Basilico C,Mansukhani A

更新日期：1994-11-01 00:00:00

abstract：:Although TRAIL is considered a potential anticancer agent, it enhances tumor progression by activating NF-κB in apoptosis-resistant cells. Cellular FLICE-like inhibitory protein (cFLIP) overexpression and caspase-8 activation have been implicated in TRAIL-induced NF-κB activation; however, the underlying mechanisms ar...

journal_title：Molecular and cellular biology

authors： Zhang L,Blackwell K,Workman LM,Chen S,Pope MR,Janz S,Habelhah H

更新日期：2015-10-01 00:00:00

abstract：:All inbred strains of mice carry the Ren-1 structural gene, which encodes the renin-1 isozyme, the classical renin activity found in kidneys. In addition, some strains carry a second renin structural gene, Ren-2, which encodes the predominantly expressed submaxillary gland renin isozyme, renin-2. Ren-1 and Ren-2 exhib...

journal_title：Molecular and cellular biology

authors： Field LJ,Philbrick WM,Howles PN,Dickinson DP,McGowan RA,Gross KW

更新日期：1984-11-01 00:00:00

abstract：:We have characterized the putative AP1 site in the backbone of pUC plasmids and found unique regulatory effects. The site, which mapped to a 19-bp region around nucleotide 37, conferred transcriptional activation by Jun or Jun/Fos that was boosted up to fivefold by unliganded thyroid hormone receptor (TR). Thyroid hor...

journal_title：Molecular and cellular biology

authors： Lopez G,Schaufele F,Webb P,Holloway JM,Baxter JD,Kushner PJ

更新日期：1993-05-01 00:00:00

abstract：:We have analyzed the 5'-flanking region of one of the genes coding for the human acute-phase protein, serum amyloid A (SAA). We found that SAA mRNA could be increased fivefold in transfected cells by treatment with phorbol 12-myristate 13-acetate (PMA). To analyze this observation further, we placed a 265-base-pair 5'...

journal_title：Molecular and cellular biology

authors： Edbrooke MR,Burt DW,Cheshire JK,Woo P

更新日期：1989-05-01 00:00:00

abstract：:N-methyl-D-aspartate receptors (NMDARs) are ligand-gated ion channels that play an important role in neuronal development, plasticity, and excitotoxicity. NMDAR antagonists are neuroprotective in animal models of neuronal diseases, and the NMDAR open-channel blocker memantine is used to treat Alzheimer's disease. In v...

journal_title：Molecular and cellular biology

authors： Kahlfuß S,Simma N,Mankiewicz J,Bose T,Lowinus T,Klein-Hessling S,Sprengel R,Schraven B,Heine M,Bommhardt U

更新日期：2014-03-01 00:00:00

abstract：:The Drosophila dopa decarboxylase gene, Ddc, is expressed in the hypoderm and in a small number of cells in the central nervous system (CNS). The unique Ddc primary transcript is alternatively spliced in these two tissues. We investigated whether Ddc splicing in the CNS is a general property of the CNS or a unique pro...

journal_title：Molecular and cellular biology

authors： Shen J,Beall CJ,Hirsh J

更新日期：1993-08-01 00:00:00

abstract：:DHCR24/seladin-1, a crucial enzyme in sterol synthesis, is of lower abundance in brain areas affected by Alzheimer's disease. While high levels of DHCR24/seladin-1 exert antiapoptotic function by conferring resistance against oxidative stress, the molecular mechanism for this protective effect is not fully understood....

journal_title：Molecular and cellular biology

authors： Kuehnle K,Crameri A,Kälin RE,Luciani P,Benvenuti S,Peri A,Ratti F,Rodolfo M,Kulic L,Heppner FL,Nitsch RM,Mohajeri MH

更新日期：2008-01-01 00:00:00

abstract：:To determine if Neu is dominant over transforming growth factor beta (TGF-beta), we crossed mouse mammary tumor virus (MMTV)-Neu mice with MMTV-TGF-beta1(S223/225) mice expressing active TGF-beta1 in the mammary gland. Bigenic (NT) and Neu-induced mammary tumors developed with a similar latency. The bigenic tumors and...

journal_title：Molecular and cellular biology

authors： Muraoka RS,Koh Y,Roebuck LR,Sanders ME,Brantley-Sieders D,Gorska AE,Moses HL,Arteaga CL

更新日期：2003-12-01 00:00:00

abstract：:Myoblast city (mbc), a member of the CDM superfamily, is essential in the Drosophila melanogaster embryo for fusion of myoblasts into multinucleate fibers. Using germ line clones in which both maternal and zygotic contributions were eliminated and rescue of the zygotic loss-of-function phenotype, we established that m...

journal_title：Molecular and cellular biology

authors： Balagopalan L,Chen MH,Geisbrecht ER,Abmayr SM

更新日期：2006-12-01 00:00:00

abstract：:Glucocorticoid-induced leucine zipper (GILZ) is a leucine zipper protein, whose expression is augmented by dexamethasone (DEX) treatment and downregulated by T-cell receptor (TCR) triggering. Stable expression of GILZ in T cells mimics some of the effects of glucocorticoid hormones (GCH) in GCH-mediated immunosuppress...

journal_title：Molecular and cellular biology

authors： Ayroldi E,Zollo O,Macchiarulo A,Di Marco B,Marchetti C,Riccardi C

更新日期：2002-11-01 00:00:00

abstract：:To evaluate the role of mitogen-activated protein (MAP) kinase and other signaling pathways in neuronal cell differentiation by basic fibroblast-derived growth factor (bFGF), we used a conditionally immortalized cell line from rat hippocampal neurons (H19-7). Previous studies have shown that activation of MAP kinase k...

journal_title：Molecular and cellular biology

authors： Kuo WL,Chung KC,Rosner MR

更新日期：1997-08-01 00:00:00

abstract：:Unique single-stranded regions of simian virus 40 DNA, phage M13 virion DNA, and several homopolymers were used as templates for the synthesis of (p)ppRNA-DNA chains by CV-1 cell DNA primase-DNA polymerase alpha. Intact RNA primers, specifically labeled with an RNA capping enzyme, were typically 6 to 8 ribonucleotides...

journal_title：Molecular and cellular biology

authors： Yamaguchi M,Hendrickson EA,DePamphilis ML

更新日期：1985-05-01 00:00:00