Mechanism of B-cell receptor-induced phosphorylation and activation of phospholipase C-gamma2.

abstract：:The extracellular matrix (ECM) promotes tissue morphogenesis, cell migration, and the differentiation of a variety of cell types. However, the mechanisms by which ECM causes differentiated gene expression have been unknown. In this report, we show that culturing the hepatocyte-derived cell line H2.35 on an ECM gel cha...

journal_title：Molecular and cellular biology

authors： DiPersio CM,Jackson DA,Zaret KS

更新日期：1991-09-01 00:00:00

abstract：:Mdm2 and MdmX are structurally related p53-binding proteins that function as critical negative regulators of p53 activity in embryonic and adult tissue. The overexpression of Mdm2 or MdmX inhibits p53 tumor suppressor functions in vitro, and the amplification of Mdm2 or MdmX is observed in human cancers retaining wild...

journal_title：Molecular and cellular biology

authors： Matijasevic Z,Steinman HA,Hoover K,Jones SN

更新日期：2008-02-01 00:00:00

abstract：:We have constructed a genomic DNA library from a methotrexate-resistant Chinese hamster ovary cell line (CHOC 400) in the cosmid vector pHC79. By utilizing a murine dihydrofolate reductase (DHFR) cDNA clone, we have identified 66 DHFR+ clones among the 11,000 colonies screened by colony hybridization. To isolate a rec...

journal_title：Molecular and cellular biology

authors： Milbrandt JD,Azizkhan JC,Greisen KS,Hamlin JL

更新日期：1983-07-01 00:00:00

abstract：:We have used in vitro mutagenesis to examine in detail the roles of two modular protein domains, SH2 and SH3, in the regulation of the Abl tyrosine kinase. As previously shown, the SH3 domain suppresses an intrinsic transforming activity of the normally nontransforming c-Abl product in vivo. We show here that this inh...

journal_title：Molecular and cellular biology

authors： Mayer BJ,Baltimore D

更新日期：1994-05-01 00:00:00

abstract：:In the budding yeast Saccharomyces cerevisiae, mutations in the essential gene CDC1 cause defects in Golgi inheritance and actin polarization. However, the biochemical function of Cdc1p is unknown. Previous work showed that cdc1 mutants accumulate intracellular Ca(2+) and display enhanced sensitivity to the extracellu...

journal_title：Molecular and cellular biology

authors： Losev E,Papanikou E,Rossanese OW,Glick BS

更新日期：2008-05-01 00:00:00

abstract：:Eukaryotic ribosome biogenesis requires the action of approximately 200 trans-acting factors and the incorporation of 79 ribosomal proteins (RPs). The delivery of RPs to preribosomes is a major challenge for the cell because RPs are often highly basic and contain intrinsically disordered regions prone to nonspecific i...

journal_title：Molecular and cellular biology

authors： Black JJ,Musalgaonkar S,Johnson AW

更新日期：2019-08-12 00:00:00

abstract：:Enamel mineralization is accompanied by the release of protons into the extracellular matrix, which is buffered to regulate the pH value in the local microenvironment. The present study aimed to investigate the role of microRNA 224 (miR-224) as a regulator of SLC4A4 and CFTR, encoding the key buffering ion transporter...

journal_title：Molecular and cellular biology

authors： Fan Y,Zhou Y,Zhou X,Sun F,Gao B,Wan M,Zhou X,Sun J,Xu X,Cheng L,Crane J,Zheng L

更新日期：2015-08-01 00:00:00

abstract：:The erect wing (ewg) locus of Drosophila melanogaster encodes a vital function important for the development of the nervous system and the indirect flight muscles. In order to understand the ewg function at a molecular level, cDNA clones were isolated. Sequence analysis of cDNAs revealed a single open reading frame (O...

journal_title：Molecular and cellular biology

authors： DeSimone SM,White K

更新日期：1993-06-01 00:00:00

abstract：:We show that the extent of transcriptional regulation of many, apparently unrelated, genes in Saccharomyces cerevisiae is dependent on RPD1 (and RPD3 [M. Vidal and R. F. Gaber, Mol. Cell. Biol. 11:6317-6327, 1991]). Genes regulated by stimuli as diverse as external signals (PHO5), cell differentiation processes (SPO11...

journal_title：Molecular and cellular biology

authors： Vidal M,Strich R,Esposito RE,Gaber RF

更新日期：1991-12-01 00:00:00

abstract：:CCN2/CTGF is a multifunctional factor that plays a crucial role in the growth and differentiation of chondrocytes. The chicken ccn2 gene is regulated not only at the transcriptional level but also by the interaction between a posttranscriptional element in the 3' untranslated region (3'-UTR) and a cofactor. In the pre...

journal_title：Molecular and cellular biology

authors： Mukudai Y,Kubota S,Kawaki H,Kondo S,Eguchi T,Sumiyoshi K,Ohgawara T,Shimo T,Takigawa M

更新日期：2008-10-01 00:00:00

abstract：:Induction of differentiation in one type of clone of mouse myeloid leukemic cells by mouse or human interleukin 6 (IL-6) and in another type of clone by mouse granulocyte-macrophage colony-stimulating factor (GM-CSF) was found to be associated with induction of IL-6 and GM-CSF mRNA and protein. The results indicated t...

journal_title：Molecular and cellular biology

authors： Shabo Y,Lotem J,Sachs L

更新日期：1989-09-01 00:00:00

abstract：:Protein phosphatase 2A (PP2A) is a multimeric serine/threonine phosphatase that carries out multiple functions. Although numerous observations suggest that PP2A plays a major role in downregulation of the mitogen-activated protein (MAP) kinase pathway, the precise mechanisms are unknown. To clarify the role of PP2A in...

journal_title：Molecular and cellular biology

authors： Ugi S,Imamura T,Ricketts W,Olefsky JM

更新日期：2002-04-01 00:00:00

abstract：:The human vitamin D-binding protein (hDBP) gene exists in a cluster of four liver-expressed genes. A minimal hDBP transgene, containing a defined set of liver-specific DNase I hypersensitive sites (HSs), is robustly expressed in mouse liver in a copy-number-dependent manner. Here we evaluate these HSs for function. De...

journal_title：Molecular and cellular biology

authors： Hiroki T,Liebhaber SA,Cooke NE

更新日期：2007-11-01 00:00:00

abstract：:We have isolated a full-length mouse cDNA encoding a lysine-rich protein of 1,131 amino acids with a calculated molecular mass of 126 kDa. The protein binds in a sequence-unspecific manner to DNA, is localized exclusively in the nucleus, and contains a putative ATP binding site and a stretch of 80 amino acids with hom...

journal_title：Molecular and cellular biology

authors： Luckow B,Bunz F,Stillman B,Lichter P,Schütz G

更新日期：1994-03-01 00:00:00

abstract：:Angiotensin II (AII), a potent vasoactive hormone, acts on numerous organs via G-protein-coupled receptors and elicits cell-specific responses. At the level of the heart, AII stimulation alters gene transcription and leads to cardiomyocyte hypertrophy. Numerous intracellular signaling pathways are activated in this pr...

journal_title：Molecular and cellular biology

authors： Wang J,Paradis P,Aries A,Komati H,Lefebvre C,Wang H,Nemer M

更新日期：2005-11-01 00:00:00

abstract：:We report the development of a novel in vivo transcription assay for trans-acting factors regulating the human gamma- and beta-globin genes. A cDNA coding for the human tissue-type plasminogen activator (t-PA) was inserted into the globin genes. Simian virus 40 small T-antigen splice and polyadenylation signals were i...

journal_title：Molecular and cellular biology

authors： Wrighton N,Grosveld F

更新日期：1988-01-01 00:00:00

abstract：:Cyclic AMP-responsive element-binding protein 3-like 3, hepatocyte specific (CREBH), is a hepatic transcription factor that functions as a key regulator of energy homeostasis. Here, we defined a regulatory CREBH posttranslational modification process, namely, lysine-specific acetylation, and its functional involvement...

journal_title：Molecular and cellular biology

authors： Kim H,Mendez R,Chen X,Fang D,Zhang K

更新日期：2015-12-01 00:00:00

abstract：:An accumulation in cells of unfolded proteins is believed to be the common signal triggering the induction of heat shock proteins (hsps). Accordingly, in Saccharomyces cerevisiae, inhibition of protein breakdown at 30 degrees C with the proteasome inhibitor MG132 caused a coordinate induction of many heat shock protei...

journal_title：Molecular and cellular biology

authors： Lee DH,Goldberg AL

更新日期：1998-01-01 00:00:00

abstract：:Translation initiation in eukaryotic cells is known to be a complex multistep process which involves numerous protein factors. Here we demonstrate that leaderless mRNAs with initiator Met-tRNA can bind directly to 80S mammalian ribosomes in the absence of initiation factors and that the complexes thus formed are fully...

journal_title：Molecular and cellular biology

authors： Andreev DE,Terenin IM,Dunaevsky YE,Dmitriev SE,Shatsky IN

更新日期：2006-04-01 00:00:00

abstract：:Two Drosophila tumor necrosis factor receptor-associated factors (TRAF), DTRAF1 and DTRAF2, are proposed to have similar functions with their mammalian counterparts as a signal mediator of cell surface receptors. However, their in vivo functions and related signaling pathways are not fully understood yet. Here, we sho...

journal_title：Molecular and cellular biology

authors： Cha GH,Cho KS,Lee JH,Kim M,Kim E,Park J,Lee SB,Chung J

更新日期：2003-11-01 00:00:00

abstract：:We previously purified and characterized functionally the Escherichia coli-expressed product of the human subgroup C adenovirus E1A 13S mRNA (B. Ferguson, N. Jones, J. Richter, and M. Rosenberg, Science 224:1343-1346, 1984; B. Krippl, B. Ferguson, M. Rosenberg, and H. Westphal, Proc. Natl. Acad. Sci. USA 81:6988-6992,...

journal_title：Molecular and cellular biology

authors： Ferguson B,Krippl B,Andrisani O,Jones N,Westphal H,Rosenberg M

更新日期：1985-10-01 00:00:00

abstract：:Little is known about the regions of RNA polymerase II (RNAPII) that are involved in the process of transcript elongation and interaction with elongation factors. One elongation factor, TFIIS, stimulates transcript elongation by binding to RNAPII and facilitating its passage through intrinsic pausing sites in vitro. I...

journal_title：Molecular and cellular biology

authors： Archambault J,Lacroute F,Ruet A,Friesen JD

更新日期：1992-09-01 00:00:00

abstract：:Pref-1/Dlk1 is made as an epidermal growth factor (EGF) repeat-containing transmembrane protein but is cleaved by tumor necrosis factor alpha converting enzyme (TACE) to generate a biologically active soluble form. Soluble Pref-1 inhibits adipocyte differentiation through the activation of extracellular signal-regulat...

journal_title：Molecular and cellular biology

authors： Wang Y,Zhao L,Smas C,Sul HS

更新日期：2010-07-01 00:00:00

abstract：:Two cis-regulatory regions are required for efficient mRNA 3'-end processing of the maize 27-kDa zein mRNA: a region containing a duplicated AAUGAA poly(A) signal and a region that is present upstream from it. Strict spatial positioning of these two regions is required for efficient mRNA 3'-end processing. Insertion o...

journal_title：Molecular and cellular biology

authors： Wu L,Ueda T,Messing J

更新日期：1994-10-01 00:00:00

abstract：:Inactivating mutations within the von Hippel-Lindau (VHL) tumor suppressor gene predispose patients to develop a variety of highly vascularized tumors. pVHL targets alpha subunits of the heterodimeric transcription factor hypoxia-inducible factor (HIF), a critical regulator of energy metabolism, angiogenesis, hematopo...

journal_title：Molecular and cellular biology

authors： Mack FA,Patel JH,Biju MP,Haase VH,Simon MC

更新日期：2005-06-01 00:00:00

abstract：:There are nearly 50 forkhead (FOX) transcription factors encoded in the human genome and, due to sharing a common DNA binding domain, they are all thought to bind to similar DNA sequences. It is therefore unclear how these transcription factors are targeted to specific chromatin regions to elicit specific biological e...

journal_title：Molecular and cellular biology

authors： Chen X,Müller GA,Quaas M,Fischer M,Han N,Stutchbury B,Sharrocks AD,Engeland K

更新日期：2013-01-01 00:00:00

abstract：:The eukaryotic SNM1 gene family has been implicated in a number of cellular pathways, including repair of DNA interstrand cross-links, involvement in VDJ recombination, repair of DNA double-strand breaks, and participation in cell cycle checkpoint pathways. In particular, mammalian SNM1 has been shown to be required i...

journal_title：Molecular and cellular biology

authors： Ahkter S,Richie CT,Zhang N,Behringer RR,Zhu C,Legerski RJ

更新日期：2005-11-01 00:00:00

abstract：:The cytokine gamma interferon (IFN-gamma) and the calcitropic steroid hormone 1,25-dihydroxyvitamin D (1,25D) are activators of macrophage immune function. In sarcoidosis, tuberculosis, and several granulomatoses, IFN-gamma induces 1,25D synthesis by macrophages and inhibits 1,25D induction of 24-hydroxylase, a key en...

journal_title：Molecular and cellular biology

authors： Vidal M,Ramana CV,Dusso AS

更新日期：2002-04-01 00:00:00

abstract：:The Drosophila melanogaster Myb-MuvB/dREAM complex (MMB/dREAM) participates in both the activation and repression of developmentally regulated genes and origins of DNA replication. Mutants in MMB subunits exhibit diverse phenotypes, including lethality, eye defects, reduced fecundity, and sterility. Here, we used P-el...

journal_title：Molecular and cellular biology

authors： Lewis PW,Sahoo D,Geng C,Bell M,Lipsick JS,Botchan MR

更新日期：2012-08-01 00:00:00

abstract：:Cooperation between STAT3 and c-Jun in driving transcription during transfection of reporter constructs is well established, and both proteins are present on some interleukin-6 (IL-6) STAT3-dependent promoters on chromosomal loci. We report that small interfering RNA knockdown of c-Jun or c-Fos diminishes IL-6 inducti...

journal_title：Molecular and cellular biology

authors： Ginsberg M,Czeko E,Müller P,Ren Z,Chen X,Darnell JE Jr

更新日期：2007-09-01 00:00:00