Abstract:
:The erect wing (ewg) locus of Drosophila melanogaster encodes a vital function important for the development of the nervous system and the indirect flight muscles. In order to understand the ewg function at a molecular level, cDNA clones were isolated. Sequence analysis of cDNAs revealed a single open reading frame (ORF) encoding a protein of 733 residues. The translational start for this ORF is a CTG codon. A 225-amino-acid region of this protein is 71% identical to the DNA binding region of the Strongylocentrotus purpuratus P3A2 DNA binding protein. Additionally, the ORF contains large acidic and basic domains characteristic of those in proteins involved in nuclear regulatory functions. Immunoblot analysis using polyclonal anti-EWG antisera generated against a bacterial fusion protein reveals a single, 116-kDa protein present throughout development, beginning at approximately stage 12 of embryogenesis, which is enriched in adult heads and absent from embryos carrying certain ewg alleles. Additionally, we show that EWG is localized specifically to the nuclei of virtually all embryonic neurons. Finally, a minigene consisting of an ewg cDNA under control of the hsp70 promoter can provide the ewg function in transgenic ewg mutant flies.
journal_name
Mol Cell Bioljournal_title
Molecular and cellular biologyauthors
DeSimone SM,White Kdoi
10.1128/mcb.13.6.3641subject
Has Abstractpub_date
1993-06-01 00:00:00pages
3641-9issue
6eissn
0270-7306issn
1098-5549journal_volume
13pub_type
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pub_type: 杂志文章
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更新日期:2004-08-01 00:00:00
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journal_title:Molecular and cellular biology
pub_type: 杂志文章
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