A flexible RNA backbone within the polypyrimidine tract is required for U2AF65 binding and pre-mRNA splicing in vivo.

abstract：:The oxidation of methanol follows a well-defined pathway and is similar for several methylotrophic yeasts. The use of methanol as the sole carbon source for the growth of Pichia pastoris stimulates the expression of a family of genes. Three methanol-responsive genes have been isolated; cDNA copies have been made from ...

journal_title：Molecular and cellular biology

authors： Ellis SB,Brust PF,Koutz PJ,Waters AF,Harpold MM,Gingeras TR

更新日期：1985-05-01 00:00:00

abstract：:The baker's yeast Saccharomyces cerevisiae expresses three homologues of the Nramp family of metal transporters: Smf1p, Smf2p, and Smf3p, encoded by SMF1, SMF2, and SMF3, respectively. Here we report a comparative analysis of the yeast Smf proteins at the levels of localization, regulation, and function of the corresp...

journal_title：Molecular and cellular biology

authors： Portnoy ME,Liu XF,Culotta VC

更新日期：2000-11-01 00:00:00

abstract：:GCN4 is a transcriptional activator of amino acid-biosynthetic genes in the yeast Saccharomyces cerevisiae. GCN2, a translational activator of GCN4 expression, contains a domain homologous to the catalytic subunit of eucaryotic protein kinases. Substitution of a highly conserved lysine residue in the kinase domain abo...

journal_title：Molecular and cellular biology

authors： Wek RC,Ramirez M,Jackson BM,Hinnebusch AG

更新日期：1990-06-01 00:00:00

abstract：:In Saccharomyces cerevisiae, the intracellular concentration of trehalose increases rapidly in response to many environmental stresses, including heat shock. These high trehalose levels have been correlated with tolerance to adverse conditions and led to the model that trehalose functions as a chemical cochaperone. He...

journal_title：Molecular and cellular biology

authors： Conlin LK,Nelson HC

更新日期：2007-02-01 00:00:00

abstract：:Negative elongation factor (NELF) is a human transcription factor complex that cooperates with DRB sensitivity-inducing factor (DSIF)/hSpt4-hSpt5 to repress elongation by RNA polymerase II (RNAPII). NELF activity is associated with five polypeptides, including NELF-A, a candidate gene product for Wolf-Hirschhorn syndr...

journal_title：Molecular and cellular biology

authors： Yamaguchi Y,Inukai N,Narita T,Wada T,Handa H

更新日期：2002-05-01 00:00:00

abstract：:Mediator is a general coactivator complex connecting transcription activators and RNA polymerase II. Recent work has shown that the nuclear receptor-interacting MED1/TRAP220 subunit of Mediator is required for peroxisome proliferator-activated receptor gamma (PPARgamma)-stimulated adipogenesis of mouse embryonic fibro...

journal_title：Molecular and cellular biology

authors： Ge K,Cho YW,Guo H,Hong TB,Guermah M,Ito M,Yu H,Kalkum M,Roeder RG

更新日期：2008-02-01 00:00:00

abstract：:We have isolated a gene, pmk1+, a third mitogen-activated protein kinase (MAPK) gene homolog from the fission yeast Schizosaccharomyces pombe. The predicted amino acid sequence shows the most homology (63 to 65% identity) to those of budding yeast Saccharomyces Mpk1 and Candida Mkc1. The Pmk1 protein contains phosphor...

journal_title：Molecular and cellular biology

authors： Toda T,Dhut S,Superti-Furga G,Gotoh Y,Nishida E,Sugiura R,Kuno T

更新日期：1996-12-01 00:00:00

abstract：:Cytochrome P450 oxidoreductase (POR) is the obligate electron donor for all microsomal cytochrome P450 enzymes, which catalyze the metabolism of a wide spectrum of xenobiotic and endobiotic compounds. Point mutations in POR have been found recently in patients with Antley-Bixler-like syndrome, which includes limb skel...

journal_title：Molecular and cellular biology

authors： Schmidt K,Hughes C,Chudek JA,Goodyear SR,Aspden RM,Talbot R,Gundersen TE,Blomhoff R,Henderson C,Wolf CR,Tickle C

更新日期：2009-05-01 00:00:00

abstract：:The signal-transducing adaptor protein 2 (STAP-2) is a recently identified adaptor protein that contains a pleckstrin homology (PH) and Src homology 2 (SH2)-like domains, as well as a proline-rich domain in its C-terminal region. In previous studies, we demonstrated that STAP-2 binds to MyD88 and IKK-alpha or IKK-beta...

journal_title：Molecular and cellular biology

authors： Ikeda O,Sekine Y,Yasui T,Oritani K,Sugiyma K,Muromoto R,Ohbayashi N,Yoshimura A,Matsuda T

更新日期：2008-08-01 00:00:00

abstract：:Using an expression cDNA cloning approach, we examined human tumor cell lines for novel oncogenes that might evade detection by conventional techniques. We isolated a transforming sequence that was highly efficient in transforming NIH 3T3 mouse fibroblasts. DNA sequence analysis identified the gene as the human homolo...

journal_title：Molecular and cellular biology

authors： Chan AM,Fleming TP,McGovern ES,Chedid M,Miki T,Aaronson SA

更新日期：1993-02-01 00:00:00

abstract：:The p53 tumor suppressor gene product is a sequence-specific DNA-binding protein that is necessary for the G1 arrest of many cell types. Consistent with its role as a cell cycle checkpoint factor, p53 has been shown to be capable of both transcriptional activation and repression. Here we show a new potential role for ...

journal_title：Molecular and cellular biology

authors： Miller SD,Farmer G,Prives C

更新日期：1995-12-01 00:00:00

abstract：:TRPV5 and TRPV6 are the most Ca2+-selective members of the transient receptor potential (TRP) family of cation channels and play a pivotal role in the maintenance of Ca2+ balance in the body. However, little is known about the mechanisms controlling the plasma membrane abundance of these channels to regulate epithelia...

journal_title：Molecular and cellular biology

authors： van de Graaf SF,Chang Q,Mensenkamp AR,Hoenderop JG,Bindels RJ

更新日期：2006-01-01 00:00:00

abstract：:The human neuroblastoma cell line CHP100 provides a useful model system in which to study the molecular mechanisms of transcriptional regulation of the low-affinity nerve growth factor receptor (NGFR) gene during neuronal development. Basic fibroblast growth factor (bFGF) induced morphological changes in CHP100 cells,...

journal_title：Molecular and cellular biology

authors： Taiji M,Taiji K,Deyerle KL,Bothwell M

更新日期：1992-05-01 00:00:00

abstract：:RNR2 encodes the small subunit of ribonucleotide reductase, the enzyme that catalyzes the first step in the pathway for the production of deoxyribonucleotides needed for DNA synthesis. RNR2 is a member of a group of genes whose activities are cell cycle regulated and that are transcriptionally induced in response to t...

journal_title：Molecular and cellular biology

authors： Elledge SJ,Davis RW

更新日期：1989-11-01 00:00:00

abstract：:The Werner syndrome protein (WRN) suppresses the loss of telomeres replicated by lagging-strand synthesis by a yet to be defined mechanism. Here, we show that whereas either WRN or the Bloom syndrome helicase (BLM) stimulates DNA polymerase δ progression across telomeric G-rich repeats, only WRN promotes sequential st...

journal_title：Molecular and cellular biology

authors： Li B,Reddy S,Comai L

更新日期：2017-01-19 00:00:00

abstract：:Though widely recognized in higher eukaryotes, the regulation of Saccharomyces cerevisiae genes transcribed by RNA polymerase II by proteins that bind within the coding sequence remains largely speculative. We have shown for the LPD1 gene, encoding lipoamide dehydrogenase, that the coding sequence between +13 and +469...

journal_title：Molecular and cellular biology

authors： Sinclair DA,Kornfeld GD,Dawes IW

更新日期：1994-01-01 00:00:00

abstract：:The phosphoinositide phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2] regulates the activity of many actin-binding proteins and as such is an important modulator of cytoskeleton organization during cell migration, for example. In migrating cells actin remodeling is tightly regulated and localized; therefore, how the ...

journal_title：Molecular and cellular biology

authors： Kisseleva M,Feng Y,Ward M,Song C,Anderson RA,Longmore GD

更新日期：2005-05-01 00:00:00

abstract：:Endothelial cells differentiate from mesoderm-derived precursors to initiate the earliest events in vascular development. Although the signaling events that regulate the successive steps of vascular development are known in some detail, the transcriptional processes that regulate the first steps in vasculogenesis are ...

journal_title：Molecular and cellular biology

authors： Wu Y,Moser M,Bautch VL,Patterson C

更新日期：2003-08-01 00:00:00

abstract：:SRC family kinases play essential roles in a variety of cellular functions, including proliferation, survival, differentiation, and apoptosis. The activities of these kinases are regulated by intramolecular interactions and by heterologous binding partners that modulate the transition between active and inactive struc...

journal_title：Molecular and cellular biology

authors： Burnham MR,Bruce-Staskal PJ,Harte MT,Weidow CL,Ma A,Weed SA,Bouton AH

更新日期：2000-08-01 00:00:00

abstract：:Mutations in the gene encoding methyl-CpG-binding protein 2 (MeCP2) lead to disrupted neuronal function and can cause the neurodevelopmental disorder Rett syndrome. MeCP2 is a transcriptional regulator that binds to methylated DNA and is most abundant in neuronal nuclei. The mechanisms by which MeCP2 regulates gene ex...

journal_title：Molecular and cellular biology

authors： Gonzales ML,Adams S,Dunaway KW,LaSalle JM

更新日期：2012-07-01 00:00:00

abstract：:Extinction of phosphoenolpyruvate carboxykinase (PCK) gene expression in hepatoma x fibroblast hybrids is mediated by a trans-acting genetic locus designated tissue-specific extinguisher 1 (TSE1). To identify PCK gene sequences required for extinction, hepatoma transfectants expressing PCK-thymidine kinase (TK) chimer...

journal_title：Molecular and cellular biology

authors： Thayer MJ,Lugo TG,Leach RJ,Fournier RE

更新日期：1990-06-01 00:00:00

abstract：:The transcriptional regulating protein of 132 kDa (TReP-132) has been identified in steroidogenic tissues, where it acts as a coactivator of steroidogenic factor 1 (SF-1). We show here that TReP-132 plays a role in the control of cell proliferation. In human HeLa cells, TReP-132 knockdown by using small interfering RN...

journal_title：Molecular and cellular biology

authors： Gizard F,Robillard R,Barbier O,Quatannens B,Faucompré A,Révillion F,Peyrat JP,Staels B,Hum DW

更新日期：2005-06-01 00:00:00

abstract：:Eukaryotic ribosome biogenesis requires the action of approximately 200 trans-acting factors and the incorporation of 79 ribosomal proteins (RPs). The delivery of RPs to preribosomes is a major challenge for the cell because RPs are often highly basic and contain intrinsically disordered regions prone to nonspecific i...

journal_title：Molecular and cellular biology

authors： Black JJ,Musalgaonkar S,Johnson AW

更新日期：2019-08-12 00:00:00

abstract：:U6 RNA genes from the trypanosomatids Crithidia fasciculata and Leptomonas seymouri have been isolated and sequenced. As in Trypanosoma brucei, the U6 RNA genes in both C. fasciculata and L. seymouri are arranged in close linkage with upstream tRNA genes. The U6 RNA sequences from C. fasciculata and L. seymouri deviat...

journal_title：Molecular and cellular biology

authors： Xu GL,Wieland B,Bindereif A

更新日期：1994-07-01 00:00:00

abstract：:The int-1 proto-oncogene is a target for insertional activation of transcription by mouse mammary tumor virus in many murine mammary tumors. Whereas no expression of int-1 is seen in normal mammary tissue, int-1 RNA can be detected in normal mice in the neural tubes of midgestation embryos and in postmeiotic spermatoc...

journal_title：Molecular and cellular biology

authors： Papkoff J

更新日期：1989-08-01 00:00:00

abstract：:The PHR1 gene of Saccharomyces cerevisiae encodes a photolyase which repairs specifically and exclusively pyrimidine dimers, the most frequent lesions induced in DNA by far-UV radiation. We have asked whether expression of PHR1 is modulated in response to UV-induced DNA damage and to DNA-damaging agents that induce le...

journal_title：Molecular and cellular biology

authors： Sebastian J,Kraus B,Sancar GB

更新日期：1990-09-01 00:00:00

abstract：:The c-Myc oncoprotein (Myc) controls cell fate by regulating gene transcription in association with a DNA-binding partner, Max. While Max lacks a transcription regulatory domain, the N terminus of Myc contains a transcription activation domain (TAD) that recruits cofactor complexes containing the histone acetyltransfe...

journal_title：Molecular and cellular biology

authors： Faiola F,Liu X,Lo S,Pan S,Zhang K,Lymar E,Farina A,Martinez E

更新日期：2005-12-01 00:00:00

abstract：:Regulation of variant surface glycoprotein (VSG) mRNA turnover in Trypanosoma brucei was studied in bloodstream forms, in procyclic cells, and during in vitro transformation of bloodstream forms to procyclic cells by approach-to-equilibrium labeling and pulse-chase experiments. Upon initiation of transformation at 27 ...

journal_title：Molecular and cellular biology

authors： Ehlers B,Czichos J,Overath P

更新日期：1987-03-01 00:00:00

abstract：:We have analyzed the structures and locations of small nuclear RNA (snRNA) precursors at various stages in their synthesis and maturation. In the nuclei of pulse-labeled Xenopus laevis oocytes, we detected snRNAs that were longer than their mature forms at their 3' ends by up to 10 nucleotides. Analysis of the 5' caps...

journal_title：Molecular and cellular biology

authors： Neuman de Vegvar HE,Dahlberg JE

更新日期：1990-07-01 00:00:00

abstract：:RNA editing in trypanosomes has been proposed to occur through transesterification or endonuclease cleavage and RNA ligation reactions. Both models involve a chimeric intermediate in which a guide RNA (gRNA) is joined through its 3' oligo(U) tail to an editing site of the corresponding mRNA. Velocity centrifugation of...

journal_title：Molecular and cellular biology

authors： Piller KJ,Decker CJ,Rusché LN,Sollner-Webb B

更新日期：1995-06-01 00:00:00