trans-spliceosomal U6 RNAs of Crithidia fasciculata and Leptomonas seymouri: deviation from the conserved ACAGAG sequence and potential base pairing with spliced leader RNA.

abstract：:Lactose or galactose induces the expression of the lactose-galactose regulon in Kluyveromyces lactis. We show here that the regulon is not induced in strains defective in LAC9. We demonstrate that this gene codes for a regulatory protein that acts in a positive manner to induce transcription. The LAC9 gene was isolate...

journal_title：Molecular and cellular biology

authors： Wray LV Jr,Witte MM,Dickson RC,Riley MI

更新日期：1987-03-01 00:00:00

abstract：:The large T antigen of simian virus 40 (SV40) is a multifunctional protein that is essential in both the virus lytic cycle and the oncogenic transformation of cells by SV40. To investigate the role of the numerous biochemical and physiological activities of T antigen in the lytic and transformation processes, we have ...

journal_title：Molecular and cellular biology

authors： Manos MM,Gluzman Y

更新日期：1984-06-01 00:00:00

abstract：:Miz1 is a member of the POZ domain/zinc finger transcription factor family. In vivo, Miz1 forms a complex with the Myc oncoprotein and recruits Myc to core promoter elements. Myc represses transcription through Miz1 binding sites. We now show that the Miz1 gene is ubiquitously expressed during mouse embryogenesis. In ...

journal_title：Molecular and cellular biology

authors： Adhikary S,Peukert K,Karsunky H,Beuger V,Lutz W,Elsässer HP,Möröy T,Eilers M

更新日期：2003-11-01 00:00:00

abstract：:In the yeast Saccharomyces cerevisiae, the CDC25 gene product activates adenylate cyclase through RAS1 and RAS2 gene products. We have recently described the cloning of a DNA fragment which suppresses the cdc25 mutation but not ras1, ras2, or cdc35 mutations. This fragment contains a 5'-truncated open reading frame wh...

journal_title：Molecular and cellular biology

authors： Damak F,Boy-Marcotte E,Le-Roscouet D,Guilbaud R,Jacquet M

更新日期：1991-01-01 00:00:00

abstract：:We have identified in and around the immunoglobulin heavy-chain enhancer two apparently distinct negative regulatory elements which repress immunoglobulin H enhancer, simian virus 40 enhancer, and heterologous promoter activity in fibroblasts but not in myeloma cells. We propose that in nonlymphoid cells, negative reg...

journal_title：Molecular and cellular biology

authors： Imler JL,Lemaire C,Wasylyk C,Wasylyk B

更新日期：1987-07-01 00:00:00

abstract：:The baker's yeast Saccharomyces cerevisiae possesses a single gene encoding heat shock transcription factor (HSF), which is required for the activation of genes that participate in stress protection as well as normal growth and viability. Yeast HSF (yHSF) contains two distinct transcriptional activation regions locate...

journal_title：Molecular and cellular biology

authors： Santoro N,Johansson N,Thiele DJ

更新日期：1998-11-01 00:00:00

abstract：:Starting with hybrid cell lines between a Chinese hamster cell EmtA mutant and a Chinese hamster cell EmtB mutant, we have constructed cell lines that are homozygous for mutant alleles at both the emtA locus and the emtB locus, by using a two-step segregation protocol. The EmtA EmtB double mutants are approximately 10...

journal_title：Molecular and cellular biology

authors： Chang S,Wasmuth JJ

更新日期：1983-05-01 00:00:00

abstract：:We generated a line of mice in which sterol regulatory element binding protein 1a (SREBP-1a) was specifically inactivated by insertional mutagenesis. Homozygous mutant mice were completely viable despite expressing SREBP-1a mRNA below 5% of normal, and there were minimal effects on expression of either SREBP-1c or -2....

journal_title：Molecular and cellular biology

authors： Im SS,Hammond LE,Yousef L,Nugas-Selby C,Shin DJ,Seo YK,Fong LG,Young SG,Osborne TF

更新日期：2009-09-01 00:00:00

abstract：:Protein 4.1G is a membrane skeletal protein found in specific subcellular structures in myelinated Schwann cells and seminiferous tubules. Here, we show that in the mouse sciatic nerve, protein 4.1G colocalized at Schmidt-Lanterman incisures (SLI) and the paranodes with a member of the membrane-associated guanylate ki...

journal_title：Molecular and cellular biology

authors： Terada N,Saitoh Y,Ohno N,Komada M,Saitoh S,Peles E,Ohno S

更新日期：2012-01-01 00:00:00

abstract：:The death-associated protein (DAP) kinase family includes three protein kinases, DAP kinase, DAP kinase-related protein 1, and ZIP kinase, which display 80% amino acid identity within their catalytic domains and are functionally linked to common subcellular changes occurring during cell death, such as the process of m...

journal_title：Molecular and cellular biology

authors： Shani G,Marash L,Gozuacik D,Bialik S,Teitelbaum L,Shohat G,Kimchi A

更新日期：2004-10-01 00:00:00

abstract：:Precursor RNA containing the adenovirus L3 polyadenylation site is assembled into a 50S complex upon incubation with HeLa nuclear extract at 30 degrees C. The cofactor and sequence requirements for 50S complex formation are similar to those of the in vitro polyadenylation reaction. Assembly of this complex requires AT...

journal_title：Molecular and cellular biology

authors： Moore CL,Skolnik-David H,Sharp PA

更新日期：1988-01-01 00:00:00

abstract：:The Rex protein of human T-cell leukemia virus type 1, like the functionally equivalent Rev protein of human immunodeficiency virus type 1, contains a leucine-rich activation domain that specifically interacts with the human nucleoporin-like Rab/hRIP cofactor. Here, this Rex sequence is shown to function also as a pro...

journal_title：Molecular and cellular biology

authors： Bogerd HP,Fridell RA,Benson RE,Hua J,Cullen BR

更新日期：1996-08-01 00:00:00

abstract：:The c-fes locus encodes a 93-kDa non-receptor protein tyrosine kinase (Fes) that regulates the growth and differentiation of hematopoietic and vascular endothelial cells. Unique to Fes is a long N-terminal sequence with two regions of strong homology to coiled-coil oligomerization domains. We introduced leucine-to-pro...

journal_title：Molecular and cellular biology

authors： Cheng HY,Schiavone AP,Smithgall TE

更新日期：2001-09-01 00:00:00

abstract：:Wrch-1, an atypical and transforming Rho GTPase, regulates cellular activities including proliferation and actin organization, but its functions and effectors remain poorly characterized. We show here that Wrch-1 distributes along the apical and basolateral membranes in MDCK cells and binds the cell polarity protein P...

journal_title：Molecular and cellular biology

authors： Brady DC,Alan JK,Madigan JP,Fanning AS,Cox AD

更新日期：2009-02-01 00:00:00

abstract：:The CACCC-box binding protein erythroid Krüppel-like factor (EKLF/KLF1) is a master regulator that directs the expression of many important erythroid genes. We have previously shown that EKLF drives transcription of the gene for a second KLF, basic Krüppel-like factor, or KLF3. We have now tested the in vivo role of K...

journal_title：Molecular and cellular biology

authors： Funnell AP,Norton LJ,Mak KS,Burdach J,Artuz CM,Twine NA,Wilkins MR,Power CA,Hung TT,Perdomo J,Koh P,Bell-Anderson KS,Orkin SH,Fraser ST,Perkins AC,Pearson RC,Crossley M

更新日期：2012-08-01 00:00:00

abstract：:The RNA polymerase III transcription initiation factor TFIIIB contains the TATA-box-binding protein (TBP) and polymerase III-specific TBP-associated factors (TAFs). Previous studies have shown that DNA oligonucleotides containing the consensus TATA-box sequence inhibit polymerase III transcription, implying that the D...

journal_title：Molecular and cellular biology

authors： McBryant SJ,Meier E,Leresche A,Sharp SJ,Wolf VJ,Gottesfeld JM

更新日期：1996-09-01 00:00:00

abstract：:The human ERCC3 gene, which corrects specifically the nucleotide excision repair defect in human xeroderma pigmentosum group B and cross-complements the repair deficiency in rodent UV-sensitive mutants of group 3, encodes a presumed DNA helicase that is identical to the p89 subunit of the general transcription factor ...

journal_title：Molecular and cellular biology

authors： Ma L,Westbroek A,Jochemsen AG,Weeda G,Bosch A,Bootsma D,Hoeijmakers JH,van der Eb AJ

更新日期：1994-06-01 00:00:00

abstract：:The Tor pathway mediates cell growth in response to nutrient availability, in part by inducing ribosomal protein (RP) gene expression via an unknown mechanism. Expression of RP genes coincides with recruitment of the Esa1 histone acetylase to RP gene promoters. We show that inhibition of Tor with rapamycin releases Es...

journal_title：Molecular and cellular biology

authors： Rohde JR,Cardenas ME

更新日期：2003-01-01 00:00:00

abstract：:Two alternative exons, BEK and K-SAM, code for part of the ligand binding site of fibroblast growth factor receptor 2. Splicing of these exons is mutually exclusive, and the choice between them is made in a tissue-specific manner. We identify here pre-mRNA sequences involved in controlling splicing of the K-SAM exon. ...

journal_title：Molecular and cellular biology

authors： Del Gatto F,Breathnach R

更新日期：1995-09-01 00:00:00

abstract：:To identify novel genes that play critical roles in mediating bone morphogenetic protein (BMP) signal pathways, we performed a yeast two-hybrid screen using Smad1 as bait. A novel mouse Krüppel-type zinc finger protein, mZnf8, was isolated. Interactions between mZnf8 and Smad proteins were further analyzed with variou...

journal_title：Molecular and cellular biology

authors： Jiao K,Zhou Y,Hogan BL

更新日期：2002-11-01 00:00:00

abstract：:Rad23 and Rpn10 play synergistic roles in the recognition of ubiquitinated proteins by the proteasome, and loss of both proteins causes growth and proteolytic defects. However, the physiological targets of Rad23 and Rpn10 have not been well defined. We report that rad23Delta rpn10Delta is unable to grow in the presenc...

journal_title：Molecular and cellular biology

authors： Chuang SM,Chen L,Lambertson D,Anand M,Kinzy TG,Madura K

更新日期：2005-01-01 00:00:00

abstract：:Xrn1p of Saccharomyces cerevisiae is a major cytoplasmic RNA turnover exonuclease which is evolutionarily conserved from yeasts to mammals. Deletion of the XRN1 gene causes pleiotropic phenotypes, which have been interpreted as indirect consequences of the RNA turnover defect. By sequence comparisons, we have identifi...

journal_title：Molecular and cellular biology

authors： Solinger JA,Pascolini D,Heyer WD

更新日期：1999-09-01 00:00:00

abstract：:We have isolated a full-length mouse cDNA encoding a lysine-rich protein of 1,131 amino acids with a calculated molecular mass of 126 kDa. The protein binds in a sequence-unspecific manner to DNA, is localized exclusively in the nucleus, and contains a putative ATP binding site and a stretch of 80 amino acids with hom...

journal_title：Molecular and cellular biology

authors： Luckow B,Bunz F,Stillman B,Lichter P,Schütz G

更新日期：1994-03-01 00:00:00

abstract：:Irradiated mice reconstituted with bone marrow cells infected with a retrovirus carrying the bcr-abl oncogene of human chronic myeloid leukemia are subject to a range of neoplastic hematopoietic diseases, both myeloid and lymphoid. Comparison of DBA/2 and C57BL/6 mice has revealed a marked strain difference in suscept...

journal_title：Molecular and cellular biology

authors： Elefanty AG,Cory S

更新日期：1992-04-01 00:00:00

abstract：:We have studied the patterns of expression of four B-type cyclins (Clbs), Clb1, Clb2, Clb3, and Clb4, and their ability to activate p34cdc28 during the mitotic and meiotic cell cycles of Saccharomyces cerevisiae. During the mitotic cell cycle, Clb3 and Clb4 were expressed and induced a kinase activity in association w...

journal_title：Molecular and cellular biology

authors： Grandin N,Reed SI

更新日期：1993-04-01 00:00:00

abstract：:Expression of the anaphase-promoting complex/cyclosome (APC/C) inhibitor Emi1 is required for the accumulation of APC/C substrates crucial for DNA synthesis and mitotic entry. We show that in vivo Emi1 expression correlates with the proliferative status of the cellular compartment and that cells lacking Emi1 undergo c...

journal_title：Molecular and cellular biology

authors： Verschuren EW,Ban KH,Masek MA,Lehman NL,Jackson PK

更新日期：2007-11-01 00:00:00

abstract：:A cDNA sequence which contains the entire coding region for human purine nucleoside phosphorylase (PNP) was recombined for selection and expression in mammalian cells. Plasmids containing either the simian virus 40 early promoter or the mouse metallothionein promoter positioned just upstream of the PNP coding sequence...

journal_title：Molecular and cellular biology

authors： McIvor RS,Goddard JM,Simonsen CC,Martin DW Jr

更新日期：1985-06-01 00:00:00

abstract：:G(1)-specific transcription in the budding yeast Saccharomyces cerevisiae depends upon SBF and MBF. Whereas inactivation of SBF-regulated genes during the G(1)/S transition depends upon mitotic B-type cyclins, inactivation of MBF has been reported to involve multiple regulators, Nrm1 and Stb1. Nrm1 is a transcriptiona...

journal_title：Molecular and cellular biology

authors： de Bruin RA,Kalashnikova TI,Wittenberg C

更新日期：2008-11-01 00:00:00

abstract：:Human MED26 was originally purified in the cofactor required for the Sp1 activation complex (CRSP) as a 70-kDa component named CRSP70. This polypeptide was specific to metazoans and the “small” form of the Mediator complex. We report here that a Drosophila melanogaster homologue of MED26 similarly interacts with other...

journal_title：Molecular and cellular biology

authors： Marr SK,Lis JT,Treisman JE,Marr MT 2nd

更新日期：2014-07-01 00:00:00

abstract：:The tumor suppressor protein ARF inhibits MDM2 to activate and stabilize p53. Recent studies provided evidence for p53-independent tumor suppression functions of ARF. For example, it has been shown that ARF induces proteolysis of certain E2F species, including E2F1. In addition, ARF relocalizes E2F1 from the nucleopla...

journal_title：Molecular and cellular biology

authors： Datta A,Nag A,Raychaudhuri P

更新日期：2002-12-01 00:00:00