trans-spliceosomal U6 RNAs of Crithidia fasciculata and Leptomonas seymouri: deviation from the conserved ACAGAG sequence and potential base pairing with spliced leader RNA.


:U6 RNA genes from the trypanosomatids Crithidia fasciculata and Leptomonas seymouri have been isolated and sequenced. As in Trypanosoma brucei, the U6 RNA genes in both C. fasciculata and L. seymouri are arranged in close linkage with upstream tRNA genes. The U6 RNA sequences from C. fasciculata and L. seymouri deviate in five and three positions, respectively, from the published T. brucei sequence. Interestingly, both C. fasciculata U6 RNA genes carry a C-->T change at the second position of the ACAGAG hexanucleotide sequence, which is important for splicing function and has been considered phylogenetically invariable. A compensatory base change of the C. fasciculata spliced leader RNA at the highly conserved 5' splice site position +5, G-->A, suggests that an interaction between the 5' splice site region and U6 RNA recently proposed for the yeast cis-splicing system may also occur in trans splicing.


Mol Cell Biol


Xu GL,Wieland B,Bindereif A




Has Abstract


1994-07-01 00:00:00












  • Physical and functional interaction between DNA ligase IIIalpha and poly(ADP-Ribose) polymerase 1 in DNA single-strand break repair.

    abstract::The repair of DNA single-strand breaks in mammalian cells is mediated by poly(ADP-ribose) polymerase 1 (PARP-1), DNA ligase IIIalpha, and XRCC1. Since these proteins are not found in lower eukaryotes, this DNA repair pathway plays a unique role in maintaining genome stability in more complex organisms. XRCC1 not only ...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Leppard JB,Dong Z,Mackey ZB,Tomkinson AE

    更新日期:2003-08-01 00:00:00

  • Novel role for cyclophilin A in regulation of chondrogenic commitment and endochondral ossification.

    abstract::Recent studies showed that cyclophilin A (CypA) promotes NF-κB/p65 nuclear translocation, resulting in enhanced NF-κB activity and altered expression of its target genes, such as the Sox9 transcriptional factor, which plays a critical role in chondrogenic differentiation and endochondral ossification. In this report, ...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Guo M,Shen J,Kwak JH,Choi B,Lee M,Hu S,Zhang X,Ting K,Soo CB,Chiu RH

    更新日期:2015-06-01 00:00:00

  • SH2B1β interacts with STAT3 and enhances fibroblast growth factor 1-induced gene expression during neuronal differentiation.

    abstract::Neurite outgrowth is an essential process during neuronal differentiation as well as neuroregeneration. Thus, understanding the molecular and cellular control of neurite outgrowth will benefit patients with neurological diseases. We have previously shown that overexpression of the signaling adaptor protein SH2B1β prom...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Chang YJ,Chen KW,Chen CJ,Lin MH,Sun YJ,Lee JL,Chiu IM,Chen L

    更新日期:2014-03-01 00:00:00

  • Circadian and light-induced transcription of clock gene Per1 depends on histone acetylation and deacetylation.

    abstract::Circadian clock genes are regulated through a transcriptional-translational feedback loop. Alterations of the chromatin structure by histone acetyltransferases and histone deacetylases (HDACs) are commonly implicated in the regulation of gene transcription. However, little is known about the transcriptional regulation...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Naruse Y,Oh-hashi K,Iijima N,Naruse M,Yoshioka H,Tanaka M

    更新日期:2004-07-01 00:00:00

  • TAZ suppresses NFAT5 activity through tyrosine phosphorylation.

    abstract::Transcriptional coactivator with PDZ-binding motif (TAZ) physically interacts with a variety of transcription factors and modulates their activities involved in cell proliferation and mesenchymal stem cell differentiation. TAZ is highly expressed in the kidney, and a deficiency of this protein results in multiple rena...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Jang EJ,Jeong H,Han KH,Kwon HM,Hong JH,Hwang ES

    更新日期:2012-12-01 00:00:00

  • L-Sox5 and Sox6 drive expression of the aggrecan gene in cartilage by securing binding of Sox9 to a far-upstream enhancer.

    abstract::The Sry-related high-mobility-group box transcription factor Sox9 recruits the redundant L-Sox5 and Sox6 proteins to effect chondrogenesis, but the mode of action of the trio remains unclear. We identify here a highly conserved 359-bp sequence 10 kb upstream of the Agc1 gene for aggrecan, a most essential cartilage pr...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Han Y,Lefebvre V

    更新日期:2008-08-01 00:00:00

  • TReP-132 controls cell proliferation by regulating the expression of the cyclin-dependent kinase inhibitors p21WAF1/Cip1 and p27Kip1.

    abstract::The transcriptional regulating protein of 132 kDa (TReP-132) has been identified in steroidogenic tissues, where it acts as a coactivator of steroidogenic factor 1 (SF-1). We show here that TReP-132 plays a role in the control of cell proliferation. In human HeLa cells, TReP-132 knockdown by using small interfering RN...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Gizard F,Robillard R,Barbier O,Quatannens B,Faucompré A,Révillion F,Peyrat JP,Staels B,Hum DW

    更新日期:2005-06-01 00:00:00

  • Co-amplification of rRNA genes with CAD genes in N-(phosphonacetyl)-L-aspartate-resistant Syrian hamster cells.

    abstract::The amplified CAD genes in N-(phosphonacetyl)-L-aspartate (PALA)-resistant Syrian hamster cells are located in an expanded chromosomal region emanating from the site of the wild-type gene at the tip of the short arm of chromosome B-9. The terminus of B-9 in PALA-sensitive cells contains a cluster of rRNA genes (i.e., ...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Wahl GM,Vitto L,Rubnitz J

    更新日期:1983-11-01 00:00:00

  • Stably integrated mouse mammary tumor virus long terminal repeat DNA requires the octamer motifs for basal promoter activity.

    abstract::In the mouse mammary tumor virus promoter, a tandem of octamer motifs, recognized by ubiquitous and tissue-restricted Oct transcription factors, is located upstream of the TATA box and next to a binding site for the transcription factor nuclear factor I (NF-I). Their function was investigated with mutant long terminal...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Buetti E

    更新日期:1994-02-01 00:00:00

  • Peroxisome proliferator-activated receptor gamma target gene encoding a novel angiopoietin-related protein associated with adipose differentiation.

    abstract::The nuclear receptor peroxisome proliferator-activated receptor gamma regulates adipose differentiation and systemic insulin signaling via ligand-dependent transcriptional activation of target genes. However, the identities of the biologically relevant target genes are largely unknown. Here we describe the isolation a...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Yoon JC,Chickering TW,Rosen ED,Dussault B,Qin Y,Soukas A,Friedman JM,Holmes WE,Spiegelman BM

    更新日期:2000-07-01 00:00:00

  • Modulation of epidermal growth factor receptor proto-oncogene transcription by a promoter site sensitive to S1 nuclease.

    abstract::The epidermal growth factor (EGF) receptor is the functional target of the mitogen EGF and the cellular homolog of the avian erythroblastosis virus erbB oncogene product. Regulation of expression of the proto-oncogene encoding the EGF receptor can be elucidated by studying the structure and function of the gene promot...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Johnson AC,Jinno Y,Merlino GT

    更新日期:1988-10-01 00:00:00

  • Binding of the termination factor Nsi1 to its cognate DNA site is sufficient to terminate RNA polymerase I transcription in vitro and to induce termination in vivo.

    abstract::Different models have been proposed explaining how eukaryotic gene transcription is terminated. Recently, Nsi1, a factor involved in silencing of ribosomal DNA (rDNA), was shown to be required for efficient termination of rDNA transcription by RNA polymerase I (Pol I) in the yeast Saccharomyces cerevisiae. Nsi1 contai...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Merkl P,Perez-Fernandez J,Pilsl M,Reiter A,Williams L,Gerber J,Böhm M,Deutzmann R,Griesenbeck J,Milkereit P,Tschochner H

    更新日期:2014-10-01 00:00:00

  • FKLF, a novel Krüppel-like factor that activates human embryonic and fetal beta-like globin genes.

    abstract::A cDNA encoding a novel Krüppel-type zinc finger protein, FKLF, was cloned from fetal globin-expressing human fetal erythroid cells. The deduced polypeptide sequence composed of 512 amino acids revealed that, like Sp1 and EKLF, FKLF has three contiguous zinc fingers at the near carboxyl-terminal end. A long amino-term...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Asano H,Li XS,Stamatoyannopoulos G

    更新日期:1999-05-01 00:00:00

  • Glucocorticoid-induced leucine zipper inhibits the Raf-extracellular signal-regulated kinase pathway by binding to Raf-1.

    abstract::Glucocorticoid-induced leucine zipper (GILZ) is a leucine zipper protein, whose expression is augmented by dexamethasone (DEX) treatment and downregulated by T-cell receptor (TCR) triggering. Stable expression of GILZ in T cells mimics some of the effects of glucocorticoid hormones (GCH) in GCH-mediated immunosuppress...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Ayroldi E,Zollo O,Macchiarulo A,Di Marco B,Marchetti C,Riccardi C

    更新日期:2002-11-01 00:00:00

  • The posttranslational processing of ras p21 is critical for its stimulation of yeast adenylate cyclase.

    abstract::Mammalian ras genes substitute for the yeast RAS gene, and their products activate adenylate cyclase in yeast cells, although the direct target protein of mammalian ras p21s remains to be identified. ras p21s undergo posttranslational processing, including prenylation, proteolysis, methylation, and palmitoylation, at ...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Horiuchi H,Kaibuchi K,Kawamura M,Matsuura Y,Suzuki N,Kuroda Y,Kataoka T,Takai Y

    更新日期:1992-10-01 00:00:00

  • Transcription of alpha-specific genes in Saccharomyces cerevisiae: DNA sequence requirements for activity of the coregulator alpha 1.

    abstract::Transcription activation of alpha-specific genes in Saccharomyces cerevisiae is regulated by two proteins, MCM1 and alpha 1, which bind to DNA sequences, called P'Q elements, found upstream of alpha-specific genes. Neither MCM1 nor alpha 1 alone binds efficiently to P'Q elements. Together, however, they bind cooperati...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Hagen DC,Bruhn L,Westby CA,Sprague GF Jr

    更新日期:1993-11-01 00:00:00

  • Induction of specific transcription by RNA polymerase III in transformed cells.

    abstract::RNA polymerase III (pol III) transcripts of the highly repeated mouse B2 gene family are increased in many oncogenically transformed murine cell lines. In cells transformed by simian virus 40, the small, cytoplasmic B2 RNAs are present at 20-fold-higher levels than in normal cells (M. R. D. Scott, K. Westphal, and P. ...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Carey MF,Singh K,Botchan M,Cozzarelli NR

    更新日期:1986-09-01 00:00:00

  • TFIIA induces conformational changes in TFIID via interactions with the basic repeat.

    abstract::DNA-binding studies with Saccharomyces cerevisiae TFIID point mutants indicated that TFIIA interacts with the basic repeat region of TFIID and induces structural changes. The latter was shown by the ability of TFIIA to compensate for TFIID point mutants defective for DNA binding. Interaction with TFIIA also rendered T...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Lee DK,DeJong J,Hashimoto S,Horikoshi M,Roeder RG

    更新日期:1992-11-01 00:00:00

  • Membrane localization of phosphatidylinositol 3-kinase is sufficient to activate multiple signal-transducing kinase pathways.

    abstract::Phosphatidylinositol (PI) 3-kinase is a cytoplasmic signaling molecule recruited to the membrane by activated growth factor receptors. The p85 subunit of PI 3-kinase links the catalytic p110 subunit to activated growth factor receptors and is required for enzymatic activity of p110. In this report, we describe the eff...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Klippel A,Reinhard C,Kavanaugh WM,Apell G,Escobedo MA,Williams LT

    更新日期:1996-08-01 00:00:00

  • Synergy between Apc min and an activated ras mutation is sufficient to induce colon carcinomas.

    abstract::Colon carcinomas appear to arise from the cumulative effect of mutations to several genes (APC, DCC, p53, ras, hMLH1, and hMSH2). By using novel colonic epithelial cell lines derived from the Immorto mouse, named the YAMC (young adult mouse colon) cell line, and an Immorto-Min mouse hybrid, named the IMCE (Immorto-Min...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: D'Abaco GM,Whitehead RH,Burgess AW

    更新日期:1996-03-01 00:00:00

  • Munc18c function is required for insulin-stimulated plasma membrane fusion of GLUT4 and insulin-responsive amino peptidase storage vesicles.

    abstract::To examine the functional role of the interaction between Munc18c and syntaxin 4 in the regulation of GLUT4 translocation in 3T3L1 adipocytes, we assessed the effects of introducing three different peptide fragments (20 to 24 amino acids) of Munc18c from evolutionarily conserved regions of the Sec1 protein family pred...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Thurmond DC,Kanzaki M,Khan AH,Pessin JE

    更新日期:2000-01-01 00:00:00

  • Progenitor stage-specific activity of a cis-acting double GATA motif for Gata1 gene expression.

    abstract::GATA1 is a master regulator of erythropoiesis, expression of which is regulated by multiple discrete cis-acting elements. In this study, we examine the activity of a promoter-proximal double GATA (dbGATA) motif, using a Gata1 bacterial artificial chromosome (BAC)-transgenic green fluorescent protein (GFP) reporter (G1...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Moriguchi T,Suzuki M,Yu L,Takai J,Ohneda K,Yamamoto M

    更新日期:2015-03-01 00:00:00

  • Mammalian DET1 regulates Cul4A activity and forms stable complexes with E2 ubiquitin-conjugating enzymes.

    abstract::DET1 (de-etiolated 1) is an essential negative regulator of plant light responses, and it is a component of the Arabidopsis thaliana CDD complex containing DDB1 and COP10 ubiquitin E2 variant. Human DET1 has recently been isolated as one of the DDB1- and Cul4A-associated factors, along with an array of WD40-containing...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Pick E,Lau OS,Tsuge T,Menon S,Tong Y,Dohmae N,Plafker SM,Deng XW,Wei N

    更新日期:2007-07-01 00:00:00

  • Multiple sequence elements and a maternal mRNA product control cdk2 RNA polyadenylation and translation during early Xenopus development.

    abstract::Cytoplasmic poly(A) elongation is one mechanism that regulates translational recruitment of maternal mRNA in early development. In Xenopus laevis, poly(A) elongation is controlled by two cis elements in the 3' untranslated regions of responsive mRNAs: the hexanucleotide AAUAAA and a U-rich structure with the general s...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Stebbins-Boaz B,Richter JD

    更新日期:1994-09-01 00:00:00

  • The hypoxia-inducible factor 1/NOR-1 axis regulates the survival response of endothelial cells to hypoxia.

    abstract::Hypoxia induces apoptosis but also triggers adaptive mechanisms to ensure cell survival. Here we show that the prosurvival effects of hypoxia-inducible factor 1 (HIF-1) in endothelial cells are mediated by neuron-derived orphan receptor 1 (NOR-1). The overexpression of NOR-1 decreased the rate of endothelial cells und...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Martorell L,Gentile M,Rius J,Rodríguez C,Crespo J,Badimon L,Martínez-González J

    更新日期:2009-11-01 00:00:00

  • Ral-specific guanine nucleotide exchange factor activity opposes other Ras effectors in PC12 cells by inhibiting neurite outgrowth.

    abstract::Ras proteins can activate at least three classes of downstream target proteins: Raf kinases, phosphatidylinositol-3 phosphate (PI3) kinase, and Ral-specific guanine nucleotide exchange factors (Ral-GEFs). In NIH 3T3 cells, activated Ral-GEFs contribute to Ras-induced cell proliferation and oncogenic transformation by ...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Goi T,Rusanescu G,Urano T,Feig LA

    更新日期:1999-03-01 00:00:00

  • Phosphorylation of mammalian eukaryotic translation initiation factor 6 and its Saccharomyces cerevisiae homologue Tif6p: evidence that phosphorylation of Tif6p regulates its nucleocytoplasmic distribution and is required for yeast cell growth.

    abstract::The synthesis of 60S ribosomal subunits in Saccharomyces cerevisiae requires Tif6p, the yeast homologue of mammalian eukaryotic translation initiation factor 6 (eIF6). In the present work, we have isolated a protein kinase from rabbit reticulocyte lysates on the basis of its ability to phosphorylate recombinant human ...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Basu U,Si K,Deng H,Maitra U

    更新日期:2003-09-01 00:00:00

  • Hematologic disease induced in BALB/c mice by a bcr-abl retrovirus is influenced by the infection conditions.

    abstract::Irradiated mice reconstituted with bone marrow cells infected with a retrovirus carrying the bcr-abl oncogene of human chronic myeloid leukemia are subject to a range of neoplastic hematopoietic diseases, both myeloid and lymphoid. Comparison of DBA/2 and C57BL/6 mice has revealed a marked strain difference in suscept...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Elefanty AG,Cory S

    更新日期:1992-04-01 00:00:00

  • The three mouse multidrug resistance (mdr) genes are expressed in a tissue-specific manner in normal mouse tissues.

    abstract::The gene responsible for multidrug resistance (mdr), which encodes the P-glycoprotein, is a member of a multigene family. We have identified distinct mdr gene transcripts encoded by three separate mdr genes in the mouse. Expression levels of each mdr gene are dramatically different in various mouse tissues. Specific m...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Croop JM,Raymond M,Haber D,Devault A,Arceci RJ,Gros P,Housman DE

    更新日期:1989-03-01 00:00:00

  • Regulated degradation of ornithine decarboxylase requires interaction with the polyamine-inducible protein antizyme.

    abstract::Intracellular degradation of vertebrate ornithine decarboxylase (ODC) is accelerated by polyamines, the products of the pathway controlled by ODC. Antizyme, a reversible, tightly binding protein inhibitor of ODC activity, is believed to be involved in this process. Mouse and Trypanosoma brucei ODCs are structurally si...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Li X,Coffino P

    更新日期:1992-08-01 00:00:00