Abstract:
:The oxidation of methanol follows a well-defined pathway and is similar for several methylotrophic yeasts. The use of methanol as the sole carbon source for the growth of Pichia pastoris stimulates the expression of a family of genes. Three methanol-responsive genes have been isolated; cDNA copies have been made from mRNAs of these genes, and the protein products from in vitro translations have been examined. The identification of alcohol oxidase as one of the cloned, methanol-regulated genes has been made by enzymatic, immunological, and sequence analyses. Methanol-regulated expression of each of these three isolated genes can be demonstrated to occur at the level of transcription. Finally, DNA subfragments of two of the methanol-responsive genomic clones from P. pastoris have been isolated and tentatively identified as containing the control regions involved in methanol regulation.
journal_name
Mol Cell Bioljournal_title
Molecular and cellular biologyauthors
Ellis SB,Brust PF,Koutz PJ,Waters AF,Harpold MM,Gingeras TRdoi
10.1128/mcb.5.5.1111subject
Has Abstractpub_date
1985-05-01 00:00:00pages
1111-21issue
5eissn
0270-7306issn
1098-5549journal_volume
5pub_type
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