An octamer motif contributes to the expression of the retinoic acid-regulated zinc finger gene Rex-1 (Zfp-42) in F9 teratocarcinoma cells.

abstract：:Expression of the avian very-low-density apolipoprotein II (apoVLDLII) gene is completely dependent on estrogen and restricted to the liver. We have identified binding sites for nonhistone nuclear proteins located between -1.96 and -2.61 kilobases. One of these sites, located at -2.6 kilobases (designated site 1), was...

journal_title：Molecular and cellular biology

authors： Hoodless PA,Roy RN,Ryan AK,Haché RJ,Vasa MZ,Deeley RG

更新日期：1990-01-01 00:00:00

abstract：:Regulatory regions of the mouse muscle creatine kinase (MCK) gene, previously discovered by analysis in cultured muscle cells, were analyzed in transgenic mice. The 206-bp MCK enhancer at nt-1256 was required for high-level expression of MCK-chloramphenicol acetyltransferase fusion genes in skeletal and cardiac muscle...

journal_title：Molecular and cellular biology

authors： Donoviel DB,Shield MA,Buskin JN,Haugen HS,Clegg CH,Hauschka SD

更新日期：1996-04-01 00:00:00

abstract：:The Cyp cell line consists of mouse cells transformed by a thermosensitive polyomavirus (Py) genome and routinely propagated at 39 degrees C. Cyp cells are readily induced to synthesize free Py DNA by being transferred to 33 degrees C. In one subclone (C12/a1/S48, or S48) of this line, such induction resulted in the i...

journal_title：Molecular and cellular biology

authors： Huberdeau D,Sylla BS,Herring-Gillam E,Bourgaux-Ramoisy D,Bourgaux P

更新日期：1985-10-01 00:00:00

abstract：:We have characterized the interactions between the TATA element and other sequence elements of a human heat shock protein 70 (hsp70) promoter by a mutational approach. Expression of a distal element of this promoter requires an intact TATA element in human cell lines. The hsp70 TATA element can be functionally replace...

journal_title：Molecular and cellular biology

authors： Greene JM,Kingston RE

更新日期：1990-04-01 00:00:00

abstract：:Ras proteins can activate at least three classes of downstream target proteins: Raf kinases, phosphatidylinositol-3 phosphate (PI3) kinase, and Ral-specific guanine nucleotide exchange factors (Ral-GEFs). In NIH 3T3 cells, activated Ral-GEFs contribute to Ras-induced cell proliferation and oncogenic transformation by ...

journal_title：Molecular and cellular biology

authors： Goi T,Rusanescu G,Urano T,Feig LA

更新日期：1999-03-01 00:00:00

abstract：:Transfection experiments with constructs containing various 5'-deleted fragments of the human lipoprotein lipase (LPL) promoter and the chloramphenicol acetyltransferase reporter gene revealed an LPL silencer element (LSE) in the region of nucleotides -225 to -81 of the LPL gene that functioned in Chinese hamster ovar...

journal_title：Molecular and cellular biology

authors： Tanuma Y,Nakabayashi H,Esumi M,Endo H

更新日期：1995-01-01 00:00:00

abstract：:The Notch receptor controls development by activating transcription of specific target genes in response to extracellular signals. The factors that control assembly of the Notch activator complex on target genes and its ability to activate transcription are not fully known. Here we show, through genetic and molecular ...

journal_title：Molecular and cellular biology

authors： Gause M,Eissenberg JC,Macrae AF,Dorsett M,Misulovin Z,Dorsett D

更新日期：2006-03-01 00:00:00

abstract：:A single-base deletion within the protein-coding region of the adenovirus type 5 early region 1A (E1A) genes, 399 bases downstream from the transcription start site, depresses transcription to 2% of the wild-type rate. Complementation studies demonstrated that this was due to two effects of the mutation: first, inacti...

journal_title：Molecular and cellular biology

authors： Osborne TF,Arvidson DN,Tyau ES,Dunsworth-Browne M,Berk AJ

更新日期：1984-07-01 00:00:00

abstract：:Saccharomyces cerevisiae dbf4 and cdc7 cell cycle mutants block initiation of DNA synthesis (i.e., are iDS mutants) at 37 degrees C and arrest the cell cycle with a 1C DNA content. Surprisingly, certain dbf4 and cdc7 strains divide their chromatin at 37 degrees C. We found that the activation of the Cdc28 mitotic prot...

journal_title：Molecular and cellular biology

authors： Toyn JH,Johnson AL,Johnston LH

更新日期：1995-10-01 00:00:00

abstract：:Oxygen radicals regulate many physiological processes, such as signaling, proliferation, and apoptosis, and thus play a pivotal role in pathophysiology and disease development. There are at least two thioredoxin reductase/thioredoxin/peroxiredoxin systems participating in the cellular defense against oxygen radicals. ...

journal_title：Molecular and cellular biology

authors： Conrad M,Jakupoglu C,Moreno SG,Lippl S,Banjac A,Schneider M,Beck H,Hatzopoulos AK,Just U,Sinowatz F,Schmahl W,Chien KR,Wurst W,Bornkamm GW,Brielmeier M

更新日期：2004-11-01 00:00:00

abstract：:Transfection of a plasmid encoding the Epstein-Barr virus (EBV) nuclear antigen 2 (EBNA2) gene confers resistance to the antiproliferative effect of alpha interferon (IFN-alpha) in EBV-negative U968 cells (P. Aman and A. von Gabain, EMBO J. 9:147-152, 1990). We studied the expression of IFN-stimulated genes (ISGs) in ...

journal_title：Molecular and cellular biology

authors： Kanda K,Decker T,Aman P,Wahlström M,von Gabain A,Kallin B

更新日期：1992-11-01 00:00:00

abstract：:The first processing step in the maturation of mouse precursor rRNA involves cleavage at nucleotide ca. +650, at the 5' border of a 200-nucleotide region that is conserved across mammals and contains the sequences that direct the processing. To identify the relevant sequence elements, we used rRNAs with small internal...

journal_title：Molecular and cellular biology

authors： Craig N,Kass S,Sollner-Webb B

更新日期：1991-01-01 00:00:00

abstract：:We previously established that the phage phiC31 integrase, a site-specific recombinase, mediates efficient integration in the human cell environment at attB and attP phage attachment sites on extrachromosomal vectors. We show here that phage attP sites inserted at various locations in human and mouse chromosomes serve...

journal_title：Molecular and cellular biology

authors： Thyagarajan B,Olivares EC,Hollis RP,Ginsburg DS,Calos MP

更新日期：2001-06-01 00:00:00

abstract：:The extracellular matrix (ECM) promotes tissue morphogenesis, cell migration, and the differentiation of a variety of cell types. However, the mechanisms by which ECM causes differentiated gene expression have been unknown. In this report, we show that culturing the hepatocyte-derived cell line H2.35 on an ECM gel cha...

journal_title：Molecular and cellular biology

authors： DiPersio CM,Jackson DA,Zaret KS

更新日期：1991-09-01 00:00:00

abstract：:RNA interference (RNAi) in animals, cosuppression in plants, and quelling in fungi are homology-dependent gene silencing mechanisms in which the introduction of either double-stranded RNA (dsRNA) or transgenes induces sequence-specific mRNA degradation. These phenomena share a common genetic and mechanistic basis. The...

journal_title：Molecular and cellular biology

authors： Catalanotto C,Pallotta M,ReFalo P,Sachs MS,Vayssie L,Macino G,Cogoni C

更新日期：2004-03-01 00:00:00

abstract：:Thrombin stimulation of platelets induces a transient increase in the specific activity of pp60c-src followed by a redistribution of pp60c-src to the Triton X-100-insoluble, cytoskeleton-rich fraction. Concomitant with the observed increase in pp60c-src activity was a rapid dephosphorylation of tyrosine 527 in 10 to 1...

journal_title：Molecular and cellular biology

authors： Clark EA,Brugge JS

更新日期：1993-03-01 00:00:00

abstract：:The product of the putative proto-oncogene bcl-3 is an I kappa B-like molecule with novel binding properties specific for a subset of the rel family of transcriptional regulators. In vitro, Bcl-3 protein specifically inhibited the DNA binding of both the homodimeric NF-kappa B p50 subunit and a closely related homolog...

journal_title：Molecular and cellular biology

authors： Nolan GP,Fujita T,Bhatia K,Huppi C,Liou HC,Scott ML,Baltimore D

更新日期：1993-06-01 00:00:00

abstract：:The proto-oncogene c-myb is essential for a controlled balance between cell growth and differentiation. Aberrant c-Myb activity has been reported for numerous human cancers, and enforced c-Myb transcription can transform cells of lymphoid origin by stimulating cellular proliferation and inhibiting apoptotic pathways. ...

journal_title：Molecular and cellular biology

authors： Nicot C,Mahieux R,Pise-Masison C,Brady J,Gessain A,Yamaoka S,Franchini G

更新日期：2001-11-01 00:00:00

abstract：:Inactivation of the tumor suppressor p53 by degradation is a mechanism utilized by cells to adapt to endoplasmic reticulum (ER) stress. However, the mechanisms of p53 destabilization by ER stress are not known. We demonstrate here that the E3 ubiquitin-ligase Hdm2 is essential for the nucleocytoplasmic transport and p...

journal_title：Molecular and cellular biology

authors： Pluquet O,Qu LK,Baltzis D,Koromilas AE

更新日期：2005-11-01 00:00:00

abstract：:The yeast mitochondrial DNA group II introns aI1 and aI2 are retroelements that insert site specifically into intronless alleles by a process called homing. Here, we used patterns of flanking marker coconversion in crosses with wild-type and mutant aI2 introns to distinguish three coexisting homing pathways: two that ...

journal_title：Molecular and cellular biology

authors： Eskes R,Liu L,Ma H,Chao MY,Dickson L,Lambowitz AM,Perlman PS

更新日期：2000-11-01 00:00:00

abstract：:The essential Saccharomyces cerevisiae gene BDP1 encodes a subunit of RNA polymerase III (Pol III) transcription factor (TFIIIB); TATA box binding protein (TBP) and Brf1 are the other subunits of this three-protein complex. Deletion analysis defined three segments of Bdp1 that are essential for viability. A central se...

journal_title：Molecular and cellular biology

authors： Ishiguro A,Kassavetis GA,Geiduschek EP

更新日期：2002-05-01 00:00:00

abstract：:Eight strains of Chinese hamster ovary (CHO) cells having an assembly-defective beta-tubulin were found among revertants of strain Cmd 4, a mutant with a conditional lethal mutation in a beta-tubulin gene (F. Cabral, M. E. Sobel, and M. M. Gottesman, Cell 20:29-36, 1980). The altered beta-tubulins in these strains hav...

journal_title：Molecular and cellular biology

authors： Boggs B,Cabral F

更新日期：1987-08-01 00:00:00

abstract：:Chicken heart mesenchymal cells do not proliferate in medium of physiological composition containing plasma (S. Balk, Proc. Natl. Acad. Sci. USA 77:6606-6610, 1980). To understand the molecular events involved in cell quiescence and in the initiation of cell division under physiological conditions, we examined the dif...

journal_title：Molecular and cellular biology

authors： Bédard PA,Balk SD,Gunther HS,Morisi A,Erikson RL

更新日期：1987-04-01 00:00:00

abstract：:Host cell cycle genes provide important functions to retroviruses and retroviruslike elements. To define some of these functions, the cell cycle dependence of transposition of the yeast retroviruslike element Ty3 was examined. Ty3 is unique among retroviruslike elements because of the specificity of its integration, w...

journal_title：Molecular and cellular biology

authors： Menees TM,Sandmeyer SB

更新日期：1994-12-01 00:00:00

abstract：:The UvsW protein of bacteriophage T4 is involved in many aspects of phage DNA metabolism, including repair, recombination, and recombination-dependent replication. UvsW has also been implicated in the repression of origin-dependent replication at late times of infection, when UvsW is normally synthesized. Two well-cha...

journal_title：Molecular and cellular biology

authors： Dudas KC,Kreuzer KN

更新日期：2001-04-01 00:00:00

abstract：:Transcription factor CREB regulates cyclic AMP (cAMP)-dependent gene expression by binding to and activating transcription from cAMP response elements (CREs) in the promoters of target genes. The transcriptional transactivation functions of CREB are activated by its phosphorylation by cAMP-dependent protein kinase A (...

journal_title：Molecular and cellular biology

authors： Vallejo M,Gosse ME,Beckman W,Habener JF

更新日期：1995-01-01 00:00:00

abstract：:Cell polarity plays a critical role in the development of all metazoans; however, the mechanisms of cell polarity and the specific role of cell polarity pathways in mammalian organisms are still poorly understood. Lethal giant larvae (Lgl) is an apical-basal polarity gene identified in Drosophila, where it functions a...

journal_title：Molecular and cellular biology

authors： Sripathy S,Lee M,Vasioukhin V

更新日期：2011-07-01 00:00:00

abstract：:The regulation of telomerase reverse transcriptase (TERT) plays an important role in the proliferative capacity and survival of cells. Here, we report that exogenously as well as endogenously induced oxidative stress leads to translocation of endogenous as well as overexpressed human TERT from the nucleus into the cyt...

journal_title：Molecular and cellular biology

authors： Haendeler J,Hoffmann J,Brandes RP,Zeiher AM,Dimmeler S

更新日期：2003-07-01 00:00:00

abstract：:We determined the nature of mutations occurring at the autosomal APRT locus in mismatch-repair-proficient and -deficient colorectal carcinoma cell lines. The analysis of mutations that result in APRT deficiency in a mismatch-repair-deficient strain of DLD-1 heterozygous for this locus enabled us to measure the rate of...

journal_title：Molecular and cellular biology

authors： Phear G,Bhattacharyya NP,Meuth M

更新日期：1996-11-01 00:00:00

abstract：:We investigated two phenotypically distinct types of diphtheria toxin-resistant mutants of Chinese hamster cells and compared their resistance with that of naturally resistant mouse cells. All are resistant due to a defect in the process of internalization and delivery of toxin to its target in the cytosol, elongation...

journal_title：Molecular and cellular biology

authors： Didsbury JR,Moehring JM,Moehring TJ

更新日期：1983-07-01 00:00:00