Mutations affecting assembly and stability of tubulin: evidence for a nonessential beta-tubulin in CHO cells.

abstract：:The eukaryotic genome is divided into chromosomal domains of distinct gene activities. Transcriptionally silent chromatin tends to encroach upon active chromatin. Barrier elements that can block the spread of silent chromatin have been documented, but the mechanisms of their function are not resolved. We show that the...

journal_title：Molecular and cellular biology

authors： Bi X,Yu Q,Sandmeier JJ,Zou Y

更新日期：2004-03-01 00:00:00

abstract：:U12-dependent introns containing alterations of the 3' splice site AC dinucleotide or alterations in the spacing between the branch site and the 3' splice site were examined for their effects on splice site selection in vivo and in vitro. Using an intron with a 5' splice site AU dinucleotide, any nucleotide could serv...

journal_title：Molecular and cellular biology

authors： Dietrich RC,Peris MJ,Seyboldt AS,Padgett RA

更新日期：2001-03-01 00:00:00

abstract：:DNA supercoiling in the chloroplast of the unicellular green alga Chlamydomonas reinhardtii was found to change with a diurnal rhythm in cells growing in alternating 12-h dark-12-h light periods. Highest and lowest DNA superhelicities occurred at the beginning and towards the end of the 12-h light periods, respectivel...

journal_title：Molecular and cellular biology

authors： Salvador ML,Klein U,Bogorad L

更新日期：1998-12-01 00:00:00

abstract：:Synemin, a 230-kilodalton polypeptide component of avian muscle and erythrocyte intermediate filaments, is also found in association with the vimentin filaments of lens tissue. In chicken lens cells, synemin is bound to the core vimentin polymer with the same 180-nm periodicity that it exhibits in erythrocytes. Its so...

journal_title：Molecular and cellular biology

authors： Granger BL,Lazarides E

更新日期：1984-10-01 00:00:00

abstract：:Previously, we described a retrovirus vector that contained two genes: a 5' gene under transcriptional control of the viral long terminal repeat and a 3' gene under transcriptional control of the herpes simplex virus thymidine kinase promoter. By using a biological assay, we found that expression of the 5' gene is sup...

journal_title：Molecular and cellular biology

authors： Emerman M,Temin HM

更新日期：1986-03-01 00:00:00

abstract：:Transcriptional activation of eukaryotic genes depends on the precise and ordered recruitment of activators, chromatin modifiers/remodelers, coactivators, and general transcription factors to the promoters of target genes. Using the human matrix metalloproteinase 9 (MMP-9) gene as a model system, we investigated the s...

journal_title：Molecular and cellular biology

authors： Ma Z,Shah RC,Chang MJ,Benveniste EN

更新日期：2004-06-01 00:00:00

abstract：:Nuclear microinjection of c-H-ras DNA induced DNA synthesis in reversibly nonproliferating quiescent human cells. The proto-oncogene and oncogene forms were equally effective inducers. In contrast, c-H-ras DNA either alone or in combination with the adenovirus E1A gene did not cause terminally nondividing senescent ce...

journal_title：Molecular and cellular biology

authors： Lumpkin CK,Knepper JE,Butel JS,Smith JR,Pereira-Smith OM

更新日期：1986-08-01 00:00:00

abstract：:Fibroblasts transformed by Fos oncogenes display increased expression of a number of genes implicated in tumor cell invasion and metastasis. In contrast to normal 208F rat fibroblasts, Fos-transformed 208F fibroblasts are growth factor independent for invasion. We demonstrate that invasion of v-Fos- or epidermal growt...

journal_title：Molecular and cellular biology

authors： Lamb RF,Hennigan RF,Turnbull K,Katsanakis KD,MacKenzie ED,Birnie GD,Ozanne BW

更新日期：1997-02-01 00:00:00

abstract：:Immunoglobulin (Ig) mu heavy-chain gene enhancer activity is mediated by multiple DNA binding proteins. Mutations of several protein binding sites in the enhancer do not affect enhancer activity significantly. This feature, termed redundancy, is thought to be due to functional compensation of the mutated sites by othe...

journal_title：Molecular and cellular biology

authors： Dang W,Nikolajczyk BS,Sen R

更新日期：1998-11-01 00:00:00

abstract：:Strong evidence indicates that endosome-localized epidermal growth factor receptor (EGFR) plays an important role in cell signaling. However, elimination of endosomal signaling does not attenuate EGF-induced physiological outcomes, arguing against physiological relevance. Recently we established a system to specifical...

journal_title：Molecular and cellular biology

authors： Pennock S,Wang Z

更新日期：2003-08-01 00:00:00

abstract：:The Werner syndrome protein (WRN) suppresses the loss of telomeres replicated by lagging-strand synthesis by a yet to be defined mechanism. Here, we show that whereas either WRN or the Bloom syndrome helicase (BLM) stimulates DNA polymerase δ progression across telomeric G-rich repeats, only WRN promotes sequential st...

journal_title：Molecular and cellular biology

authors： Li B,Reddy S,Comai L

更新日期：2017-01-19 00:00:00

abstract：:The tumor necrosis factor alpha (TNF-alpha) gene is rapidly activated by lipopolysaccharide (LPS). Here, we show that extracellular signal-regulated kinase (ERK) kinase activity but not calcineurin phosphatase activity is required for LPS-stimulated TNF-alpha gene expression. In LPS-stimulated macrophages, the ERK sub...

journal_title：Molecular and cellular biology

authors： Tsai EY,Falvo JV,Tsytsykova AV,Barczak AK,Reimold AM,Glimcher LH,Fenton MJ,Gordon DC,Dunn IF,Goldfeld AE

更新日期：2000-08-01 00:00:00

abstract：:The Son of sevenless proteins (Sos) are guanine nucleotide exchange factors involved in the activation of Ras by cytoplasmic and receptor tyrosine kinases. Growth factor stimulation rapidly induces the phosphorylation of Sos on multiple serine and threonine sites. Previous studies have demonstrated that growth factor-...

journal_title：Molecular and cellular biology

authors： Corbalan-Garcia S,Yang SS,Degenhardt KR,Bar-Sagi D

更新日期：1996-10-01 00:00:00

abstract：:Following a proliferative phase of variable duration, most normal somatic cells enter a growth arrest state known as replicative senescence. In addition to telomere shortening, a variety of environmental insults and signaling imbalances can elicit phenotypes closely resembling senescence. We used p53(-/-) and p21(-/-)...

journal_title：Molecular and cellular biology

authors： Wei W,Hemmer RM,Sedivy JM

更新日期：2001-10-01 00:00:00

abstract：:The RHO1 gene encodes a homolog of the mammalian RhoA small GTP-binding protein in the yeast Saccharomyces cerevisiae. Rho1p is localized at the growth site and is required for bud formation. The RHO1(G22S, D125N) mutation is a temperature-sensitive and dominant negative mutation of RHO1, and a multicopy suppressor of...

journal_title：Molecular and cellular biology

authors： Hirano H,Tanaka K,Ozaki K,Imamura H,Kohno H,Hihara T,Kameyama T,Hotta K,Arisawa M,Watanabe T,Qadota H,Ohya Y,Takai Y

更新日期：1996-08-01 00:00:00

abstract：:To study the relationship between oncogenesis by v-src and normal cellular signalling pathways, we determined the effects of v-src on 3T3-TNR9 cells, a Swiss 3T3 variant which does not respond mitogenically to tumor promoters such as 12-O-tetradecanoyl-phorbol-13-acetate (TPA). We found that src was unable to transfor...

journal_title：Molecular and cellular biology

authors： Nori M,Shawver LK,Weber MJ

更新日期：1990-08-01 00:00:00

abstract：:A number of deletion mutants were isolated, including 5', 3', and internal deletions in the 5'-flanking region of the human cellular oncogene related to the Harvey sarcoma virus (c-H-ras), and their transforming activities were examined in NIH 3T3 cells. DNA sequences which could not be detected without losing transfo...

journal_title：Molecular and cellular biology

authors： Honkawa H,Masahashi W,Hashimoto S,Hashimoto-Gotoh T

更新日期：1987-08-01 00:00:00

abstract：:Previous studies have identified novel modifications in the core fold domain of histone H2B, but relatively little is known about the function of these putative histone modification sites. We have mutated core modifiable residues that are conserved in Saccharomyces cerevisiae histone H2B and characterized the effects ...

journal_title：Molecular and cellular biology

authors： Kyriss MN,Jin Y,Gallegos IJ,Sanford JA,Wyrick JJ

更新日期：2010-07-01 00:00:00

abstract：:U6 RNA genes from the trypanosomatids Crithidia fasciculata and Leptomonas seymouri have been isolated and sequenced. As in Trypanosoma brucei, the U6 RNA genes in both C. fasciculata and L. seymouri are arranged in close linkage with upstream tRNA genes. The U6 RNA sequences from C. fasciculata and L. seymouri deviat...

journal_title：Molecular and cellular biology

authors： Xu GL,Wieland B,Bindereif A

更新日期：1994-07-01 00:00:00

abstract：:To study repair of DNA double-strand breaks (DSBs) in mammalian chromosomes, we designed DNA substrates containing a thymidine kinase (TK) gene disrupted by the 18-bp recognition site for yeast endonuclease I-SceI. Some substrates also contained a second defective TK gene sequence to serve as a genetic donor in recomb...

journal_title：Molecular and cellular biology

authors： Lin Y,Lukacsovich T,Waldman AS

更新日期：1999-12-01 00:00:00

abstract：:Interleukin 1 (IL-1) induces the synthesis of kappa immunoglobulin light chains and the expression of surface immunoglobulin in the murine pre-B-cell line 70Z/3 (J. G. Giri, P. W. Kincade, and S. B. Mizel, J. Immunol. 132:223-228, 1984). In the present study, we found that these effects of IL-1 are mimicked by cyclic ...

journal_title：Molecular and cellular biology

authors： Shirakawa F,Chedid M,Suttles J,Pollok BA,Mizel SB

更新日期：1989-03-01 00:00:00

abstract：:The Drosophila melanogaster Myb-MuvB/dREAM complex (MMB/dREAM) participates in both the activation and repression of developmentally regulated genes and origins of DNA replication. Mutants in MMB subunits exhibit diverse phenotypes, including lethality, eye defects, reduced fecundity, and sterility. Here, we used P-el...

journal_title：Molecular and cellular biology

authors： Lewis PW,Sahoo D,Geng C,Bell M,Lipsick JS,Botchan MR

更新日期：2012-08-01 00:00:00

abstract：:Vimentin is a tissue-specific, developmentally regulated member of the intermediate filament protein family normally expressed in cells of mesenchymal origin. Transcription factors which recognize specific cis-acting elements of the chicken gene include Sp-1 and the 95-kDa silencer protein which binds to a 40-bp silen...

journal_title：Molecular and cellular biology

authors： Stover DM,Zehner ZE

更新日期：1992-05-01 00:00:00

abstract：:The Cyp cell line consists of mouse cells transformed by a thermosensitive polyomavirus (Py) genome and routinely propagated at 39 degrees C. Cyp cells are readily induced to synthesize free Py DNA by being transferred to 33 degrees C. In one subclone (C12/a1/S48, or S48) of this line, such induction resulted in the i...

journal_title：Molecular and cellular biology

authors： Huberdeau D,Sylla BS,Herring-Gillam E,Bourgaux-Ramoisy D,Bourgaux P

更新日期：1985-10-01 00:00:00

abstract：:p53 not only functions as a transcription factor but also has a direct, apoptogenic role at the mitochondria. We have discovered that DNA damage-induced p53-Bcl2 binding is associated with decreased Bcl2-Bax interaction and increased apoptotic cell death in a mechanism regulated by Bcl2's flexible loop regulatory doma...

journal_title：Molecular and cellular biology

authors： Deng X,Gao F,Flagg T,Anderson J,May WS

更新日期：2006-06-01 00:00:00

abstract：:Mos is a germ cell-specific serine/threonine protein kinase that activates mitogen-activated protein kinase (MAPK) through MAPK kinase (MKK). In Xenopus oocytes, Mos synthesis is required for progesterone-induced activation of MAPK and maturation promoting factor. Injection of Mos or active MAPK causes mitotic arrest ...

journal_title：Molecular and cellular biology

authors： Chen M,Cooper JA

更新日期：1995-09-01 00:00:00

abstract：:The erb A oncogene is a dominant negative allele of a thyroid hormone receptor gene and acts in the cancer cell by encoding a transcriptional repressor. We demonstrate here that the DNA sequence recognition properties of the oncogenic form of the erb A protein are significantly altered from those of the normal thyroid...

journal_title：Molecular and cellular biology

authors： Chen H,Smit-McBride Z,Lewis S,Sharif M,Privalsky ML

更新日期：1993-04-01 00:00:00

abstract：:The Src homology 2-containing phosphotyrosine phosphatase (SHP2) is primarily a positive effector of receptor tyrosine kinase signaling. However, the molecular mechanism by which SHP2 effects its biological function is unknown. In this report, we provide evidence that defines the molecular mechanism and site of action...

journal_title：Molecular and cellular biology

authors： Agazie YM,Hayman MJ

更新日期：2003-11-01 00:00:00

abstract：:Imprinting of the linked and oppositely expressed mouse H19 and Igf2 genes requires a 2-kb differentially methylated domain (DMD) that is located 2 kb upstream of H19. This element is postulated to function as a methylation-sensitive insulator. Here we test whether an additional sequence 5' of H19 is required for H19 ...

journal_title：Molecular and cellular biology

authors： Thorvaldsen JL,Mann MR,Nwoko O,Duran KL,Bartolomei MS

更新日期：2002-04-01 00:00:00

abstract：:The imprinted expression of the H19 and Igf2 genes in the mouse is controlled by an imprinting control center (ICR) whose activity is regulated by parent-of-origin differences in methylation. The only protein that has been implicated in ICR function is the zinc-finger protein CTCF, which binds at multiple sites within...

journal_title：Molecular and cellular biology

authors： Katz DJ,Beer MA,Levorse JM,Tilghman SM

更新日期：2005-05-01 00:00:00