Mitogenic effects of the proto-oncogene and oncogene forms of c-H-ras DNA in human diploid fibroblasts.

abstract：:Vascular endothelial growth factor (VEGF) is a key angiogenic factor expressed under restricted nutrient and oxygen conditions in most solid tumors. The expression of VEGF under hypoxic conditions requires transcription through activated hypoxia-inducible factor 1 (HIF-1), increased mRNA stability, and facilitated tra...

journal_title：Molecular and cellular biology

authors： Vumbaca F,Phoenix KN,Rodriguez-Pinto D,Han DK,Claffey KP

更新日期：2008-01-01 00:00:00

abstract：:Mos is a germ cell-specific serine/threonine protein kinase that activates mitogen-activated protein kinase (MAPK) through MAPK kinase (MKK). In Xenopus oocytes, Mos synthesis is required for progesterone-induced activation of MAPK and maturation promoting factor. Injection of Mos or active MAPK causes mitotic arrest ...

journal_title：Molecular and cellular biology

authors： Chen M,Cooper JA

更新日期：1995-09-01 00:00:00

abstract：:In Saccharomyces cerevisiae, pseudouridine formation in the middle position of the tRNA(Tyr) anticodon (psi 35) is dependent on the presence of the intron in the tRNA(Tyr) gene (Johnson and Abelson, Nature 302:681-687, 1983). Drosophila melanogaster tRNA(Tyr) genes contain introns of three size classes: 20 or 21 base ...

journal_title：Molecular and cellular biology

authors： Choffat Y,Suter B,Behra R,Kubli E

更新日期：1988-08-01 00:00:00

abstract：:Mutagenesis studies have demonstrated the requirement for the CD28-responsive element (CD28RE) within the interleukin-2 (IL-2) promoter for transcriptional upregulation by CD28. Here, we demonstrate that CD28 responsiveness is conferred by a composite element containing both the CD28RE and the NF-IL-2B AP-1 sites (RE/...

journal_title：Molecular and cellular biology

authors： Shapiro VS,Truitt KE,Imboden JB,Weiss A

更新日期：1997-07-01 00:00:00

abstract：:The C proteins (C1 and C2) of HeLa 40S heterogeneous nuclear ribonucleoprotein particles copurify under native conditions as a stable complex with a fixed molar protein ratio (S.F. Barnett, W.M. LeStourgeon, and D.L. Friedman, J. Biochem. Biophys. Methods 16:87-97, 1988). Gel filtration chromatography and velocity sed...

journal_title：Molecular and cellular biology

authors： Barnett SF,Friedman DL,LeStourgeon WM

更新日期：1989-02-01 00:00:00

abstract：:Tyrosine phosphorylation of CAS (Crk-associated substrate, p130(Cas)) has been implicated as a key signaling step in integrin control of normal cellular behaviors, including motility, proliferation, and survival. Aberrant CAS tyrosine phosphorylation may contribute to cell transformation by certain oncoproteins, inclu...

journal_title：Molecular and cellular biology

authors： Ruest PJ,Shin NY,Polte TR,Zhang X,Hanks SK

更新日期：2001-11-01 00:00:00

abstract：:The 3' regulatory region (3' RR) of the murine immunoglobulin heavy chain (IgH) locus contains multiple DNase I-hypersensitive (hs) sites. Proximal sites hs3A, hs1.2, and hs3B are located in an extensive palindromic region and together with hs4 are associated with enhancers involved in the expression and class switch ...

journal_title：Molecular and cellular biology

authors： Giambra V,Volpi S,Emelyanov AV,Pflugh D,Bothwell AL,Norio P,Fan Y,Ju Z,Skoultchi AI,Hardy RR,Frezza D,Birshtein BK

更新日期：2008-10-01 00:00:00

abstract：:The yeast mitochondrial DNA group II introns aI1 and aI2 are retroelements that insert site specifically into intronless alleles by a process called homing. Here, we used patterns of flanking marker coconversion in crosses with wild-type and mutant aI2 introns to distinguish three coexisting homing pathways: two that ...

journal_title：Molecular and cellular biology

authors： Eskes R,Liu L,Ma H,Chao MY,Dickson L,Lambowitz AM,Perlman PS

更新日期：2000-11-01 00:00:00

abstract：:A 249-nucleotide coding region instability determinant (CRD) destabilizes c-myc mRNA. Previous experiments identified a CRD-binding protein (CRD-BP) that appears to protect the CRD from endonuclease cleavage. However, it was unclear why a CRD-BP is required to protect a well-translated mRNA whose coding region is cove...

journal_title：Molecular and cellular biology

authors： Lemm I,Ross J

更新日期：2002-06-01 00:00:00

abstract：:The Capn5 gene was inactivated by homologous recombination in ES cells that subsequently colonized the germ line of mice. The targeted mutation integrated a lacZ expression cassette into the Capn5 gene, allowing the expression of Capn5 mRNA to be examined in detail in heterozygous animals. Expression was observed in e...

journal_title：Molecular and cellular biology

authors： Franz T,Winckler L,Boehm T,Dear TN

更新日期：2004-02-01 00:00:00

abstract：:Analysis of the p50 and p65 subunits of the NF-kappa B transcription factor complex has revealed that both proteins can interact with related DNA sequences through either homo- or heterodimer formation. In addition, the product of the proto-oncogene c-rel can bind to similar DNA motifs by itself or as a heterodimer wi...

journal_title：Molecular and cellular biology

authors： Kunsch C,Ruben SM,Rosen CA

更新日期：1992-10-01 00:00:00

abstract：:SLC5A8 is a putative tumor suppressor that is inactivated in more than 10 different types of cancer, but neither the oncogenic signaling responsible for SLC5A8 inactivation nor the functional relevance of SLC5A8 loss to tumor growth has been elucidated. Here, we identify oncogenic HRAS (HRAS(G12V)) as a potent mediato...

journal_title：Molecular and cellular biology

authors： Elangovan S,Pathania R,Ramachandran S,Ananth S,Padia RN,Srinivas SR,Babu E,Hawthorn L,Schoenlein PV,Boettger T,Smith SB,Prasad PD,Ganapathy V,Thangaraju M

更新日期：2013-10-01 00:00:00

abstract：:Although globin mRNAs are considered prototypes of highly stable messages, the mechanisms responsible for their longevity remain largely undefined. As an initial step in identifying potential cis-acting elements or structures which contribute to their stability, we analyzed the defect in expression of a naturally occu...

journal_title：Molecular and cellular biology

authors： Weiss IM,Liebhaber SA

更新日期：1994-12-01 00:00:00

abstract：:Mutations in the SRA1 or SRA3 gene eliminate the requirement for either RAS gene (RAS1 or RAS2) in Saccharomyces cerevisiae. We cloned SRA1 and SRA3 and determined their DNA sequences. SRA1 encodes the regulatory subunit of the cyclic AMP (cAMP)-dependent protein kinase and therefore is identical to REG1 and BCY1. Thi...

journal_title：Molecular and cellular biology

authors： Cannon JF,Tatchell K

更新日期：1987-08-01 00:00:00

abstract：:GATA-3 is a zinc finger transcription factor which is expressed in a highly restricted and strongly conserved tissue distribution pattern in vertebrate organisms, specifically, in a subset of hematopoietic cells, in cells within the central and peripheral nervous systems, in the kidney, and in placental trophoblasts. ...

journal_title：Molecular and cellular biology

authors： Yang Z,Gu L,Romeo PH,Bories D,Motohashi H,Yamamoto M,Engel JD

更新日期：1994-03-01 00:00:00

abstract：:TEL1 is important in Saccharomyces cerevisiae telomere maintenance, and its kinase activity is required. Tel1p associates with telomeres in vivo, is enriched at short telomeres, and enhances the binding of telomerase components to short telomeres. However, it is unclear how the kinase activity and telomere association...

journal_title：Molecular and cellular biology

authors： Ma Y,Greider CW

更新日期：2009-09-01 00:00:00

abstract：:The gene coding for the most abundant antifreeze protein (AFP) in the winter flounder was placed downstream of the Drosophila melanogaster hsp70 promoter and introduced into the D. melanogaster germ line by P-element-mediated transformation. In each of six transgenic strains tested, heat shock treatment induced the ex...

journal_title：Molecular and cellular biology

authors： Rancourt DE,Walker VK,Davies PL

更新日期：1987-06-01 00:00:00

abstract：:RNA interference (RNAi) in animals, cosuppression in plants, and quelling in fungi are homology-dependent gene silencing mechanisms in which the introduction of either double-stranded RNA (dsRNA) or transgenes induces sequence-specific mRNA degradation. These phenomena share a common genetic and mechanistic basis. The...

journal_title：Molecular and cellular biology

authors： Catalanotto C,Pallotta M,ReFalo P,Sachs MS,Vayssie L,Macino G,Cogoni C

更新日期：2004-03-01 00:00:00

abstract：:Mutation in the REC2 gene of Ustilago maydis leads to defects in DNA repair, recombination, and meiosis. Analysis of the primary sequence of the Rec2 protein reveals a region with significant homology to bacterial RecA protein and to the yeast recombination proteins Dmc1, Rad51, and Rad57. This homologous region in th...

journal_title：Molecular and cellular biology

authors： Rubin BP,Ferguson DO,Holloman WK

更新日期：1994-09-01 00:00:00

abstract：:We have previously identified an 11-base DNA sequence, 5'-G-G-T-A-C-C-T-A-A-C-C-3' (simian virus 40 [SV40] map position 294 to 304), which is important in the control of SV40 late RNA expression in vitro and in vivo (Brady et al., Cell 31:625-633, 1982). We report here the identification of another domain of the SV40 ...

journal_title：Molecular and cellular biology

authors： Brady J,Radonovich M,Thoren M,Das G,Salzman NP

更新日期：1984-01-01 00:00:00

abstract：:The alpha 2 protein of the yeast Saccharomyces cerevisiae normally represses a set of cell-type-specific genes (the a-specific genes) that are transcribed by RNA polymerase II. In this study, we determined whether alpha 2 can affect transcription by other RNA polymerases. We find that alpha 2 can repress transcription...

journal_title：Molecular and cellular biology

authors： Herschbach BM,Johnson AD

更新日期：1993-07-01 00:00:00

abstract：:The Ras pathway is critical for the development and function of T lymphocytes. The stimulation of this GTPase in T cells occurs primarily through the Vav1- and phospholipase C-gamma1-dependent activation of RasGRP1, a diacylglycerol-responsive Ras GDP/GTP exchange factor. Here, we show that a second exchange factor, R...

journal_title：Molecular and cellular biology

authors： Ruiz S,Santos E,Bustelo XR

更新日期：2007-12-01 00:00:00

abstract：:Thrombospondins are a family of stress-inducible secreted glycoproteins that underlie tissue remodeling. We recently reported that thrombospondin-4 (Thbs4) has a critical intracellular function, regulating the adaptive endoplasmic reticulum (ER) stress pathway through activating transcription factor 6α (Atf6α). In the...

journal_title：Molecular and cellular biology

authors： Brody MJ,Schips TG,Vanhoutte D,Kanisicak O,Karch J,Maliken BD,Blair NS,Sargent MA,Prasad V,Molkentin JD

更新日期：2015-10-12 00:00:00

abstract：:Chemical carcinogenesis in mouse skin has been useful in delineating the molecular events that underlie squamous cell carcinoma progression. A late event in this progression, the epithelial-to-mesenchymal transition (EMT), is characterized by the loss of epithelial markers and the presence of mesenchymal markers. One ...

journal_title：Molecular and cellular biology

authors： Hulboy DL,Matrisian LM,Crawford HC

更新日期：2001-08-01 00:00:00

abstract：:The baker's yeast Saccharomyces cerevisiae possesses a single gene encoding heat shock transcription factor (HSF), which is required for the activation of genes that participate in stress protection as well as normal growth and viability. Yeast HSF (yHSF) contains two distinct transcriptional activation regions locate...

journal_title：Molecular and cellular biology

authors： Santoro N,Johansson N,Thiele DJ

更新日期：1998-11-01 00:00:00

abstract：:The p53 tumor suppressor gene product is a sequence-specific DNA-binding protein that is necessary for the G1 arrest of many cell types. Consistent with its role as a cell cycle checkpoint factor, p53 has been shown to be capable of both transcriptional activation and repression. Here we show a new potential role for ...

journal_title：Molecular and cellular biology

authors： Miller SD,Farmer G,Prives C

更新日期：1995-12-01 00:00:00

abstract：:Aryl hydrocarbon receptor (AhR), a ligand-activated transcription factor, is known to mediate a wide variety of pharmacological and toxicological effects caused by polycyclic aromatic hydrocarbons. Recent studies have revealed that AhR is involved in the normal development and homeostasis of many organs. Here, we demo...

journal_title：Molecular and cellular biology

authors： Sekine H,Mimura J,Oshima M,Okawa H,Kanno J,Igarashi K,Gonzalez FJ,Ikuta T,Kawajiri K,Fujii-Kuriyama Y

更新日期：2009-12-01 00:00:00

abstract：:Abundant approximately 28-nucleotide RNAs that are thought to direct histone H3 lysine 9 (H3K9) methylation and promote the elimination of nearly 15 Mbp of DNA from the developing somatic genome are generated during Tetrahymena thermophila conjugation. To identify the protein(s) that generates these small RNAs, we stu...

journal_title：Molecular and cellular biology

authors： Malone CD,Anderson AM,Motl JA,Rexer CH,Chalker DL

更新日期：2005-10-01 00:00:00

abstract：:The Msh2-Msh3 heterodimer recognizes various DNA mispairs, including loops of DNA ranging from 1 to 14 nucleotides and some base-base mispairs. Homology modeling of the mispair-binding domain (MBD) of Msh3 using the related Msh6 MBD revealed that mismatch recognition must be different, even though the MBD folds must b...

journal_title：Molecular and cellular biology

authors： Dowen JM,Putnam CD,Kolodner RD

更新日期：2010-07-01 00:00:00

abstract：:The abundance of the mRNA for human triosephosphate isomerase (TPI) is decreased to approximately 20% of normal by frameshift and nonsense mutations that cause translation to terminate at a nonsense codon within the first three-fourths of the reading frame. Results of previous studies inhibiting RNA synthesis with act...

journal_title：Molecular and cellular biology

authors： Cheng J,Maquat LE

更新日期：1993-03-01 00:00:00