Abstract:
:Mutation in the REC2 gene of Ustilago maydis leads to defects in DNA repair, recombination, and meiosis. Analysis of the primary sequence of the Rec2 protein reveals a region with significant homology to bacterial RecA protein and to the yeast recombination proteins Dmc1, Rad51, and Rad57. This homologous region in the U. maydis Rec2 protein was found to be functionally sensitive to mutation, lending support to the hypothesis that Rec2 has a functional RecA-like domain essential for activity in recombination and repair. Homologous recombination between plasmid and chromosomal DNA sequences is reduced substantially in the rec2 mutant following transformation. The frequency can be restored to a level approaching, but not exceeding, that observed in the wild-type strain if transformation is performed with cells containing multiple copies of REC2.
journal_name
Mol Cell Bioljournal_title
Molecular and cellular biologyauthors
Rubin BP,Ferguson DO,Holloman WKdoi
10.1128/mcb.14.9.6287subject
Has Abstractpub_date
1994-09-01 00:00:00pages
6287-96issue
9eissn
0270-7306issn
1098-5549journal_volume
14pub_type
杂志文章abstract::Transcriptional silencing at the budding yeast silent mating type (HM) loci and telomeric DNA regions requires Sir2, a conserved NAD-dependent histone deacetylase, Sir3, Sir4, histones H3 and H4, and several DNA-binding proteins. Silencing at the yeast ribosomal DNA (rDNA) repeats requires a complex containing Sir2, N...
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pub_type: 杂志文章
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更新日期:1996-01-01 00:00:00
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更新日期:2013-03-01 00:00:00
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pub_type: 杂志文章
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更新日期:2002-11-01 00:00:00
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