Abstract:
:Inactivation of the TATA-binding protein-containing complex TFIIIB contributes to the mitotic repression of RNA polymerase III transcription, both in frogs and in humans (J. M. Gottesfeld, V. J. Wolf, T. Dang, D. J. Forbes, and P. Hartl, Science 263:81-84, 1994; R. J. White, T. M. Gottlieb, C. S. Downes, and S. P. Jackson, Mol. Cell. Biol. 15:1983-1992, 1995). Using extracts of synchronized proliferating HeLa cells, we show that TFIIIB activity remains low during the early part of G1 phase and increases only gradually as cells approach S phase. As a result, the transcription of all class III genes tested is significantly less active in early G1 than it is in S or G2 phase, both in vitro and in vivo. The increased activity of TFIIIB as cells progress through interphase appears to be due to changes in the TATA-binding protein-associated components of this complex. The data suggest that TFIIIB is an important target for the cell cycle regulation of RNA polymerase III transcription during both mitosis and interphase of actively proliferating HeLa cells.
journal_name
Mol Cell Bioljournal_title
Molecular and cellular biologyauthors
White RJ,Gottlieb TM,Downes CS,Jackson SPdoi
10.1128/mcb.15.12.6653subject
Has Abstractpub_date
1995-12-01 00:00:00pages
6653-62issue
12eissn
0270-7306issn
1098-5549journal_volume
15pub_type
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journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/mcb.8.7.2955
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pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/mcb.15.7.3945
更新日期:1995-07-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:1991-12-01 00:00:00
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更新日期:1995-02-01 00:00:00
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更新日期:1999-05-01 00:00:00
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doi:10.1128/mcb.13.12.7522
更新日期:1993-12-01 00:00:00
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journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/mcb.6.1.15
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pub_type: 杂志文章
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更新日期:1999-07-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/mcb.6.7.2482
更新日期:1986-07-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:2003-07-01 00:00:00
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pub_type: 杂志文章
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更新日期:1989-09-01 00:00:00