Abstract:
:Eucaryotic initiation factor 4A (eIF-4A) is a member of a family of proteins believed to be involved in the ATP-dependent melting of RNA secondary structure. These proteins contain a derivative of the consensus ATP-binding site AXXGXGKT. To assess the importance of the consensus amino acid sequence in eIF-4A for ATP binding, we mutated the consensus amino-proximal glycine and lysine to isoleucine and asparagine, respectively. The effect of the mutations was examined by UV-induced cross-linking of [alpha-32P]dATP to eIF-4A. Mutation of the lysine residue (but not of the glycine residue) resulted in the loss of [alpha-32P]dATP cross-linking to eIF-4A, suggesting that the lysine is an important determinant in ATP binding to eIF-4A.
journal_name
Mol Cell Bioljournal_title
Molecular and cellular biologyauthors
Rozen F,Pelletier J,Trachsel H,Sonenberg Ndoi
10.1128/mcb.9.9.4061subject
Has Abstractpub_date
1989-09-01 00:00:00pages
4061-3issue
9eissn
0270-7306issn
1098-5549journal_volume
9pub_type
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pub_type: 杂志文章
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更新日期:1995-07-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:2007-05-01 00:00:00
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pub_type: 杂志文章
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更新日期:2003-12-01 00:00:00
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journal_title:Molecular and cellular biology
pub_type: 杂志文章,收录出版
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pub_type: 杂志文章
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journal_title:Molecular and cellular biology
pub_type: 杂志文章
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更新日期:1994-08-01 00:00:00
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journal_title:Molecular and cellular biology
pub_type: 杂志文章
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更新日期:2006-08-01 00:00:00
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journal_title:Molecular and cellular biology
pub_type: 杂志文章
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更新日期:1995-11-01 00:00:00