Extramitochondrial citrate synthase activity in bakers' yeast.

abstract：:Wnt signaling is thought to be mediated via interactions between beta-catenin and members of the LEF-1/TCF family of transcription factors. Here we study the mechanism of transcriptional regulation by LEF-1 in response to a Wnt-1 signal under conditions of endogenous beta-catenin in NIH 3T3 cells, and we examine wheth...

journal_title：Molecular and cellular biology

authors： Hsu SC,Galceran J,Grosschedl R

更新日期：1998-08-01 00:00:00

abstract：:Saccharomyces cerevisiae contains several prion elements, which are epigenetically transmitted as self-perpetuating protein conformations. One such prion is [SWI + ], whose protein determinant is Swi1, a subunit of the SWI/SNF chromatin-remodeling complex. We previously rep...

journal_title：Molecular and cellular biology

authors： Valtierra S,Du Z,Li L

更新日期：2017-09-26 00:00:00

abstract：:The biosynthesis, phosphorylation, and degradation of the epidermal growth factor (EGF) receptor were examined in normal human fibroblasts. The receptor was initially synthesized as an Mr = 160,000 immature form which matured to an Mr = 170,000 form in a monensin-sensitive manner. Tunicamycin treatment led to the accu...

journal_title：Molecular and cellular biology

authors： Decker SJ

更新日期：1984-09-01 00:00:00

abstract：:The tumor necrosis factor alpha (TNF-alpha) gene is rapidly activated by lipopolysaccharide (LPS). Here, we show that extracellular signal-regulated kinase (ERK) kinase activity but not calcineurin phosphatase activity is required for LPS-stimulated TNF-alpha gene expression. In LPS-stimulated macrophages, the ERK sub...

journal_title：Molecular and cellular biology

authors： Tsai EY,Falvo JV,Tsytsykova AV,Barczak AK,Reimold AM,Glimcher LH,Fenton MJ,Gordon DC,Dunn IF,Goldfeld AE

更新日期：2000-08-01 00:00:00

abstract：:Pref-1/Dlk1 is made as an epidermal growth factor (EGF) repeat-containing transmembrane protein but is cleaved by tumor necrosis factor alpha converting enzyme (TACE) to generate a biologically active soluble form. Soluble Pref-1 inhibits adipocyte differentiation through the activation of extracellular signal-regulat...

journal_title：Molecular and cellular biology

authors： Wang Y,Zhao L,Smas C,Sul HS

更新日期：2010-07-01 00:00:00

abstract：:To examine the functional role of the interaction between Munc18c and syntaxin 4 in the regulation of GLUT4 translocation in 3T3L1 adipocytes, we assessed the effects of introducing three different peptide fragments (20 to 24 amino acids) of Munc18c from evolutionarily conserved regions of the Sec1 protein family pred...

journal_title：Molecular and cellular biology

authors： Thurmond DC,Kanzaki M,Khan AH,Pessin JE

更新日期：2000-01-01 00:00:00

abstract：:The Polycomb group (PcG) of proteins represses homeotic gene expression through the assembly of multiprotein complexes on key regulatory elements. The mechanisms mediating complex assembly have remained enigmatic since most PcG proteins fail to bind DNA. We now demonstrate that the human PcG protein dinG interacts wit...

journal_title：Molecular and cellular biology

authors： Tuckfield A,Clouston DR,Wilanowski TM,Zhao LL,Cunningham JM,Jane SM

更新日期：2002-03-01 00:00:00

abstract：:Human CRK protein is a homolog of the chicken v-crk oncogene product and consists mostly of src homology region 2 (SH2) and SH3, which are shared by many proteins, in particular those involved in signal transduction. SH2 has been shown to bind specifically to phosphotyrosine-containing peptides. We report here that bo...

journal_title：Molecular and cellular biology

authors： Tanaka S,Hattori S,Kurata T,Nagashima K,Fukui Y,Nakamura S,Matsuda M

更新日期：1993-07-01 00:00:00

abstract：:To analyze determinants within the Na,K-ATPase alpha subunit that contribute to differential ouabain sensitivity, we constructed and expressed a panel of chimeric cDNA molecules between ouabain-resistant and ouabain-sensitive alpha subunit cDNAs. When introduced into ouabain-sensitive monkey CV-1 cells, ouabain-resist...

journal_title：Molecular and cellular biology

authors： Emanuel JR,Graw S,Housman D,Levenson R

更新日期：1989-09-01 00:00:00

abstract：:The lactogenic hormones, i.e., prolactin and glucocorticoids, act in concert to stimulate transcription factors responsible for hormone-dependent milk protein gene expression. In the mammary gland, prolactin activates Stat5a and Stat5b and glucocorticoids activate the glucocorticoid receptor (GR). Immunoprecipitation ...

journal_title：Molecular and cellular biology

authors： Cella N,Groner B,Hynes NE

更新日期：1998-04-01 00:00:00

abstract：:The E2F1, -2, and -3 transcription factors are key downstream targets of the retinoblastoma protein (pRB) tumor suppressor that drive expression of proliferation-associated genes. Here we use mutant mouse strains to investigate E2F3's role in vivo. We show that E2F3 is essential for embryonic viability in the pure 129...

journal_title：Molecular and cellular biology

authors： Cloud JE,Rogers C,Reza TL,Ziebold U,Stone JR,Picard MH,Caron AM,Bronson RT,Lees JA

更新日期：2002-04-01 00:00:00

abstract：:Ribosome biogenesis is a highly regulated process ensuring that cell growth (increase in biomass) is coordinated with cell proliferation. The formation of eukaryotic ribosomes is a multistep process initiated by the transcription and processing of rRNA in the nucleolus. Concomitant with this, several preribosomal part...

journal_title：Molecular and cellular biology

authors： Castle CD,Cassimere EK,Lee J,Denicourt C

更新日期：2010-09-01 00:00:00

abstract：:The transcriptional regulating protein of 132 kDa (TReP-132) has been identified in steroidogenic tissues, where it acts as a coactivator of steroidogenic factor 1 (SF-1). We show here that TReP-132 plays a role in the control of cell proliferation. In human HeLa cells, TReP-132 knockdown by using small interfering RN...

journal_title：Molecular and cellular biology

authors： Gizard F,Robillard R,Barbier O,Quatannens B,Faucompré A,Révillion F,Peyrat JP,Staels B,Hum DW

更新日期：2005-06-01 00:00:00

abstract：:RAG1 and RAG2 catalyze the first DNA cleavage steps in V(D)J recombination. We demonstrate that the isolated central domain of RAG1 has inherent single-stranded (ss) DNA cleavage activity, which does not require, but is enhanced by, RAG2. The central domain, therefore, contains the active-site residues necessary to pe...

journal_title：Molecular and cellular biology

authors： De P,Peak MM,Rodgers KK

更新日期：2004-08-01 00:00:00

abstract：:Entry into mitosis requires the activity of the Cdc2 kinase. Cdc2 associates with the B-type cyclins, and the Cdc2-cyclin B heterodimer is in turn regulated by phosphorylation. Phosphorylation of threonine 161 is required for the Cdc2-cyclin B complex to be catalytically active, whereas phosphorylation of threonine 14...

journal_title：Molecular and cellular biology

authors： Liu F,Stanton JJ,Wu Z,Piwnica-Worms H

更新日期：1997-02-01 00:00:00

abstract：:CCN2/CTGF is a multifunctional factor that plays a crucial role in the growth and differentiation of chondrocytes. The chicken ccn2 gene is regulated not only at the transcriptional level but also by the interaction between a posttranscriptional element in the 3' untranslated region (3'-UTR) and a cofactor. In the pre...

journal_title：Molecular and cellular biology

authors： Mukudai Y,Kubota S,Kawaki H,Kondo S,Eguchi T,Sumiyoshi K,Ohgawara T,Shimo T,Takigawa M

更新日期：2008-10-01 00:00:00

abstract：:Precise regulation of cellular proliferation, differentiation, and senescence results in the continuous renewal of the intestinal epithelium with maintenance of a highly ordered tissue architecture. Here we show that an intestine-specific homeobox gene, Cdx2, is a transcription factor that regulates both proliferation...

journal_title：Molecular and cellular biology

authors： Suh E,Traber PG

更新日期：1996-02-01 00:00:00

abstract：:Simian virus 40 (SV40) large tumor antigen (T antigen) has been shown to inhibit p53-dependent transcription by preventing p53 from binding to its cognate cis element. Data presented in this report provide the first direct functional evidence that T antigen, under certain conditions, may also repress p53-dependent tra...

journal_title：Molecular and cellular biology

authors： Sheppard HM,Corneillie SI,Espiritu C,Gatti A,Liu X

更新日期：1999-04-01 00:00:00

abstract：:The C-terminal domain of the RNA polymerase II largest subunit (the Rpb1 CTD) is composed of tandem heptad repeats of the consensus sequence Y(1)S(2)P(3)T(4)S(5)P(6)S(7). We reported previously that Thr 4 is phosphorylated and functions in histone mRNA 3'-end formation in chicken DT40 cells. Here, we have extended our...

journal_title：Molecular and cellular biology

authors： Hsin JP,Xiang K,Manley JL

更新日期：2014-07-01 00:00:00

abstract：:The c-myc proto-oncogene encodes a transcription factor that participates in the regulation of cellular proliferation, differentiation, and apoptosis. Ectopic overexpression of c-Myc has been shown to sensitize cells to apoptosis. We report here that cells lacking c-Myc activity due to disruption of the c-myc gene by ...

journal_title：Molecular and cellular biology

authors： Adachi S,Obaya AJ,Han Z,Ramos-Desimone N,Wyche JH,Sedivy JM

更新日期：2001-08-01 00:00:00

abstract：:"Neutropenia" refers to deficient numbers of neutrophils, the most abundant type of white blood cell. Two main forms of inherited neutropenia are cyclic neutropenia, in which neutrophil counts oscillate with a 21-day frequency, and severe congenital neutropenia, in which static neutropenia may evolve at times into leu...

journal_title：Molecular and cellular biology

authors： Salipante SJ,Rojas ME,Korkmaz B,Duan Z,Wechsler J,Benson KF,Person RE,Grimes HL,Horwitz MS

更新日期：2009-08-01 00:00:00

abstract：:To gain insight into the role of Myc family oncoproteins and their associated protein Max in vertebrate growth and development, we sought to identify homologs in the zebra fish (Brachydanio rerio). A combination of a polymerase chain reaction-based cloning strategy and low-stringency hybridization screening allowed fo...

journal_title：Molecular and cellular biology

authors： Schreiber-Agus N,Horner J,Torres R,Chiu FC,DePinho RA

更新日期：1993-05-01 00:00:00

abstract：:Previous analyses have identified related cytoplasmic Lsm1-7p and nuclear Lsm2-8p complexes. Here we report that mature heat shock and MET mRNAs that are trapped in the nucleus due to a block in mRNA export were strongly stabilized in strains lacking Lsm6p or the nucleus-specific Lsm8p protein but not by the absence o...

journal_title：Molecular and cellular biology

authors： Kufel J,Bousquet-Antonelli C,Beggs JD,Tollervey D

更新日期：2004-11-01 00:00:00

abstract：:Kinase-negative mutants of S49 mouse lymphoma cells, which lack detectable catalytic (C) subunit of cyclic AMP-dependent protein kinase, nevertheless contain cytoplasmic mRNAs for the two major forms of C subunit, C alpha and C beta. Investigation of the metabolism of C subunits in wild-type and mutant cells was under...

journal_title：Molecular and cellular biology

authors： Steinberg RA

更新日期：1991-02-01 00:00:00

abstract：:We have cloned and characterized the gene encoding the late histone H1-beta subtype from the sea urchin Strongylocentrotus purpuratus. The gene contains all of the upstream sequence homologies previously seen in late H1-gamma genes. The expression of H1-beta mRNA is coordinated with that of H1-gamma mRNA, and like H1-...

journal_title：Molecular and cellular biology

authors： Lai ZC,Childs G

更新日期：1988-04-01 00:00:00

abstract：:Spt6 is a highly conserved histone chaperone that interacts directly with both RNA polymerase II and histones to regulate gene expression. To gain a comprehensive understanding of the roles of Spt6, we performed genome-wide analyses of transcription, chromatin structure, and histone modifications in a Schizosaccharomy...

journal_title：Molecular and cellular biology

authors： DeGennaro CM,Alver BH,Marguerat S,Stepanova E,Davis CP,Bähler J,Park PJ,Winston F

更新日期：2013-12-01 00:00:00

abstract：:Mitogen-activated protein (MAP) kinase phosphatase 1 (MKP-1) has been shown to play a critical role in mediating the feedback control of MAP kinase cascades in a variety of cellular processes, including proliferation and stress responsiveness. Although MKP-1 expression is induced by a broad array of extracellular stim...

journal_title：Molecular and cellular biology

authors： Li J,Gorospe M,Hutter D,Barnes J,Keyse SM,Liu Y

更新日期：2001-12-01 00:00:00

abstract：:We have cloned CDC9, the structural gene for Saccharomyces cerevisiae DNA ligase, and investigated its transcriptional regulation both as a function of cell cycle stage and after UV irradiation. The steady-state level of DNA ligase mRNA increases at least fourfold in late G1, after the completion of start but before S...

journal_title：Molecular and cellular biology

authors： Peterson TA,Prakash L,Prakash S,Osley MA,Reed SI

更新日期：1985-01-01 00:00:00

abstract：:Mdm2, a regulator of the tumor suppressor p53, is frequently overexpressed in human malignancies. Mdm2 also has unresolved, p53-independent functions that contribute to tumorigenesis. Here, we show that increased Mdm2 expression induced chromosome/chromatid breaks and delayed DNA double-strand break repair in cells la...

journal_title：Molecular and cellular biology

authors： Bouska A,Lushnikova T,Plaza S,Eischen CM

更新日期：2008-08-01 00:00:00

abstract：:Cyclic AMP-responsive element-binding protein 3-like 3, hepatocyte specific (CREBH), is a hepatic transcription factor that functions as a key regulator of energy homeostasis. Here, we defined a regulatory CREBH posttranslational modification process, namely, lysine-specific acetylation, and its functional involvement...

journal_title：Molecular and cellular biology

authors： Kim H,Mendez R,Chen X,Fang D,Zhang K

更新日期：2015-12-01 00:00:00