Mdm2 promotes genetic instability and transformation independent of p53.

abstract：:Several Epstein-Barr virus (EBV)-negative Burkitt lymphoma-derived cell lines (for example, BL41 and Ramos) are extremely sensitive to genotoxic drugs despite being functionally null for the tumor suppressor p53. They rapidly undergo apoptosis, largely from G(2)/M of the cell cycle. 5-bromo-2'-deoxyuridine labeling ex...

journal_title：Molecular and cellular biology

authors： Wade M,Allday MJ

更新日期：2000-02-01 00:00:00

abstract：:To investigate the mechanism of action of volatile anesthetics, we are studying mutants of the yeast Saccharomyces cerevisiae that have altered sensitivity to isoflurane, a widely used clinical anesthetic. Several lines of evidence from these studies implicate a role for ubiquitin metabolism in cellular response to vo...

journal_title：Molecular and cellular biology

authors： Wolfe D,Reiner T,Keeley JL,Pizzini M,Keil RL

更新日期：1999-12-01 00:00:00

abstract：:Gene expression programs required for differentiation depend on both DNA-bound transcription factors and surrounding histone modifications. Expression of the basic helix-loop-helix (bHLH) protein NeuroD1 is restricted to endocrine cells in the gastrointestinal (GI) tract, where it is important for endocrine differenti...

journal_title：Molecular and cellular biology

authors： Ray SK,Li HJ,Metzger E,Schüle R,Leiter AB

更新日期：2014-06-01 00:00:00

abstract：:p53 not only functions as a transcription factor but also has a direct, apoptogenic role at the mitochondria. We have discovered that DNA damage-induced p53-Bcl2 binding is associated with decreased Bcl2-Bax interaction and increased apoptotic cell death in a mechanism regulated by Bcl2's flexible loop regulatory doma...

journal_title：Molecular and cellular biology

authors： Deng X,Gao F,Flagg T,Anderson J,May WS

更新日期：2006-06-01 00:00:00

abstract：:Mitochondria are indispensable organelles implicated in multiple aspects of cellular processes, including tumorigenesis. Heat shock proteins play a critical regulatory role in accurately delivering the nucleus-encoded proteins through membrane-bound presequence translocase (Tim23 complex) machinery. Although altered e...

journal_title：Molecular and cellular biology

authors： Sinha D,Srivastava S,Krishna L,D'Silva P

更新日期：2014-05-01 00:00:00

abstract：:The immunosuppressant and anticancer drug rapamycin works by inducing inhibitory protein complexes with the kinase mTOR, an important regulator of growth and proliferation. The obligatory accessory partner of rapamycin is believed to be FK506-binding protein 12 (FKBP12). Here we show that rapamycin complexes of larger...

journal_title：Molecular and cellular biology

authors： März AM,Fabian AK,Kozany C,Bracher A,Hausch F

更新日期：2013-04-01 00:00:00

abstract：:In Saccharomyces cerevisiae, COX5a and COX5b encode two distinct forms of cytochrome c oxidase subunit V, Va and Vb, respectively. To determine the relative contribution of COX5a and COX5b to cytochrome c oxidase function, we have disrupted each gene. Cytochrome c oxidase activity levels and respiration rates of strai...

journal_title：Molecular and cellular biology

authors： Trueblood CE,Poyton RO

更新日期：1987-10-01 00:00:00

abstract：:Many selectable mutants of somatic Chinese hamster cells have been described, but very few of the mutations have been mapped to specific chromosomes. We have utilized the microcell-mediated gene transfer technique to establish the location of three selectable genetic markers on chromosome 2 of Chinese hamster. Microce...

journal_title：Molecular and cellular biology

authors： Worton R,Duff C,Flintoff W

更新日期：1981-04-01 00:00:00

abstract：:Gene targeting is a very powerful tool for studying mammalian development and physiology and for creating models of human diseases. In many instances, however, it is desirable to study different modifications of a target gene, but this is limited by the generally low frequency of homologous recombination in mammalian ...

journal_title：Molecular and cellular biology

authors： Cohen-Tannoudji M,Robine S,Choulika A,Pinto D,El Marjou F,Babinet C,Louvard D,Jaisser F

更新日期：1998-03-01 00:00:00

abstract：:A potential p120 GTPase-activating protein (RasGAP) effector, G3BP (RasGAP Src homology 3 [SH3] binding protein), was previously identified based on its ability to bind the SH3 domain of RasGAP. Here we show that G3BP colocalizes and physically interacts with RasGAP at the plasma membrane of serum-stimulated but not q...

journal_title：Molecular and cellular biology

authors： Gallouzi IE,Parker F,Chebli K,Maurier F,Labourier E,Barlat I,Capony JP,Tocque B,Tazi J

更新日期：1998-07-01 00:00:00

abstract：:The mSin3A-histone deacetylase corepressor is a multiprotein complex that is recruited by DNA binding transcriptional repressors. Sin3 has four paired amphipathic alpha helices (PAH1 to -4) that are protein-protein interaction motifs and is the scaffold upon which the complex assembles. We identified a novel mSin3A-in...

journal_title：Molecular and cellular biology

authors： Yochum GS,Ayer DE

更新日期：2001-07-01 00:00:00

abstract：:Protein 4.1G is a membrane skeletal protein found in specific subcellular structures in myelinated Schwann cells and seminiferous tubules. Here, we show that in the mouse sciatic nerve, protein 4.1G colocalized at Schmidt-Lanterman incisures (SLI) and the paranodes with a member of the membrane-associated guanylate ki...

journal_title：Molecular and cellular biology

authors： Terada N,Saitoh Y,Ohno N,Komada M,Saitoh S,Peles E,Ohno S

更新日期：2012-01-01 00:00:00

abstract：:We investigated the possible interactions between pp39mos and p34cdc2 kinase in NIH 3T3 cells transformed by c-mosxe. pp39mos is coprecipitated with p34cdc2 when using either anti-PSTAIR antibody or p13suc1-Sepharose beads. Likewise, p34cdc2 is coprecipitated with pp39mos when using anti-mos antibody. However, pp39mos...

journal_title：Molecular and cellular biology

authors： Zhou R,Daar I,Ferris DK,White G,Paules RS,Vande Woude G

更新日期：1992-08-01 00:00:00

abstract：:Despite detailed knowledge of the components of the spindle assembly checkpoint, a molecular explanation of how cells die after prolonged spindle checkpoint activation, and thus how microtubule inhibitors and other antimitotic drugs ultimately elicit their lethal effects, has yet to emerge. Mitotically arrested cells ...

journal_title：Molecular and cellular biology

authors： Terrano DT,Upreti M,Chambers TC

更新日期：2010-02-01 00:00:00

abstract：:S1 mapping of the termination region in the ribosomal DNA from a thermophilic fungus, Thermomyces lanuginosus, revealed three distinct termini corresponding to the mature 25S rRNA, a precursor that is 19 nucleotides longer and corresponds to the 37S precursor in yeast cells, and a putative termination site at +96 that...

journal_title：Molecular and cellular biology

authors： Walker K,Wong WM,Nazar RN

更新日期：1990-01-01 00:00:00

abstract：:The first intron of the human collagen alpha 1(I) gene contains several positively and negatively acting elements. We have studied the transcription of collagen-human growth hormone fusion genes, containing deletions and rearrangements of collagen intronic sequences, by transient transfection of chick tendon fibroblas...

journal_title：Molecular and cellular biology

authors： Bornstein P,McKay J,Liska DJ,Apone S,Devarayalu S

更新日期：1988-11-01 00:00:00

abstract：:The single-copy gene encoding the alpha subunit of glycoprotein hormones is expressed in the pituitaries of all mammals and in the placentas of only primates and horses. We have systematically analyzed the promoter-regulatory elements of the human and bovine alpha-subunit genes to elucidate the molecular mechanisms un...

journal_title：Molecular and cellular biology

authors： Bokar JA,Keri RA,Farmerie TA,Fenstermaker RA,Andersen B,Hamernik DL,Yun J,Wagner T,Nilson JH

更新日期：1989-11-01 00:00:00

abstract：:We report the cloning and functional analysis of a complete clone for the third member of the mouse mdr gene family, mdr3. Nucleotide and predicted amino acid sequence analyses showed that the three mouse mdr genes encode highly homologous membrane glycoproteins, which share the same length (1,276 residues), the same ...

journal_title：Molecular and cellular biology

authors： Devault A,Gros P

更新日期：1990-04-01 00:00:00

abstract：:The transcriptional factor Snail1 is a repressor of E-cadherin (CDH1) gene expression essential for triggering epithelial-mesenchymal transition. Snail1 represses CDH1, directly binding its promoter and inducing the synthesis of the Zeb1 repressor. In this article, we show that repression of CDH1 by Snail1, but not by...

journal_title：Molecular and cellular biology

authors： Herranz N,Pasini D,Díaz VM,Francí C,Gutierrez A,Dave N,Escrivà M,Hernandez-Muñoz I,Di Croce L,Helin K,García de Herreros A,Peiró S

更新日期：2008-08-01 00:00:00

abstract：:In cystic fibrosis (CF), epithelial cells are unable to normally up-regulate apical membrane Cl- secretion in response to agents which increase cyclic AMP, but they do increase Cl- secretion in response to increases in intracellular Ca2+. Since intracellular divalent cations regulate the expression of many genes, we h...

journal_title：Molecular and cellular biology

authors： Bargon J,Trapnell BC,Chu CS,Rosenthal ER,Yoshimura K,Guggino WB,Dalemans W,Pavirani A,Lecocq JP,Crystal RG

更新日期：1992-04-01 00:00:00

abstract：:In the yeast Saccharomyces cerevisiae, the CDC25 gene product activates adenylate cyclase through RAS1 and RAS2 gene products. We have recently described the cloning of a DNA fragment which suppresses the cdc25 mutation but not ras1, ras2, or cdc35 mutations. This fragment contains a 5'-truncated open reading frame wh...

journal_title：Molecular and cellular biology

authors： Damak F,Boy-Marcotte E,Le-Roscouet D,Guilbaud R,Jacquet M

更新日期：1991-01-01 00:00:00

abstract：:Translation initiation in eukaryotic cells is known to be a complex multistep process which involves numerous protein factors. Here we demonstrate that leaderless mRNAs with initiator Met-tRNA can bind directly to 80S mammalian ribosomes in the absence of initiation factors and that the complexes thus formed are fully...

journal_title：Molecular and cellular biology

authors： Andreev DE,Terenin IM,Dunaevsky YE,Dmitriev SE,Shatsky IN

更新日期：2006-04-01 00:00:00

abstract：:The structural maintenance of chromosomes (Smc) family members Smc5 and Smc6 are both essential in budding and fission yeasts. Yeast smc5/6 mutants are hypersensitive to DNA damage, and Smc5/6 is recruited to HO-induced double-strand breaks (DSBs), facilitating intersister chromatid recombinational repair. To determin...

journal_title：Molecular and cellular biology

authors： Stephan AK,Kliszczak M,Dodson H,Cooley C,Morrison CG

更新日期：2011-04-01 00:00:00

abstract：:We examined the effects of 18 h of incubation of Chinese hamster ovary (CHO K1) cells with cycloheximide, hydroxyurea, and aphidicolin. Treatment of cells with cycloheximide alone at a concentration adequate to inhibit DNA synthesis to less than 10% of control was significantly less cytotoxic and clastogenic than trea...

journal_title：Molecular and cellular biology

authors： Sherwood SW,Schumacher RI,Schimke RT

更新日期：1988-07-01 00:00:00

abstract：:Acquisition of the ability to produce gamma interferon (IFN-gamma) is a fundamental property of memory T cells and enables one subset (T helper 1 [TH1]) to deliver its effector functions. To examine regulation of IFN-gamma gene expression in a model system which recapitulates TH1 differentiation, we prepared reporter ...

journal_title：Molecular and cellular biology

authors： Aune TM,Penix LA,Rincón MR,Flavell RA

更新日期：1997-01-01 00:00:00

abstract：:Transcription of the c-rel proto-oncogene was induced transiently when resting mouse NIH 3T3 fibroblasts were stimulated with serum or phorbol-12-myristate-13-acetate. Addition of cycloheximide increased the steady-state levels of c-rel mRNA. These results indicate that c-rel is another member of the early-response ge...

journal_title：Molecular and cellular biology

authors： Bull P,Hunter T,Verma IM

更新日期：1989-11-01 00:00:00

abstract：:Granulocyte-macrophage colony-stimulating factor (GM-CSF) stimulates myeloid progenitor cell proliferation and enhances the function of terminally differentiated effector cells. Interleukin-3 (IL-3) stimulation results in the proliferation and maturation of early bone marrow progenitor cells. These activities are medi...

journal_title：Molecular and cellular biology

authors： Sakamoto KM,Fraser JK,Lee HJ,Lehman E,Gasson JC

更新日期：1994-09-01 00:00:00

abstract：:Plasmids containing the hormone regulatory element of mouse mammary tumor virus linked to the thymidine kinase promoter of herpes simplex virus and the reporter gene chloramphenicol acetyltransferase of Escherichia coli respond to glucocorticoids and progestins when transfected into appropriate cells. In the human mam...

journal_title：Molecular and cellular biology

authors： Piña B,Haché RJ,Arnemann J,Chalepakis G,Slater EP,Beato M

更新日期：1990-02-01 00:00:00

abstract：:AIRE is a transcriptional activator that directs the ectopic expression of many tissue-specific genes in medullary thymic epithelial cells, which plays an important role in the negative selection of autoreactive T cells. However, its mechanism of action remains poorly understood. In this study, we found that AIRE regu...

journal_title：Molecular and cellular biology

authors： Oven I,Brdicková N,Kohoutek J,Vaupotic T,Narat M,Peterlin BM

更新日期：2007-12-01 00:00:00

abstract：:Multidrug resistance (MDR) to different cytotoxic compounds in the yeast Saccharomyces cerevisiae can arise from overexpression of the Pdr5 (Sts1, Ydr1, or Lem1) ATP-binding cassette (ABC) multidrug transporter. We have raised polyclonal antibodies recognizing the yeast Pdr5 ABC transporter to study its biogenesis and...

journal_title：Molecular and cellular biology

authors： Egner R,Mahé Y,Pandjaitan R,Kuchler K

更新日期：1995-11-01 00:00:00