Unraveling the intricate organization of mammalian mitochondrial presequence translocases: existence of multiple translocases for maintenance of mitochondrial function.

abstract：:The c-myc proto-oncogene encodes a transcription factor that participates in the regulation of cellular proliferation, differentiation, and apoptosis. Ectopic overexpression of c-Myc has been shown to sensitize cells to apoptosis. We report here that cells lacking c-Myc activity due to disruption of the c-myc gene by ...

journal_title：Molecular and cellular biology

authors： Adachi S,Obaya AJ,Han Z,Ramos-Desimone N,Wyche JH,Sedivy JM

更新日期：2001-08-01 00:00:00

abstract：:We have examined the possible role of adenosine 3',5'-phosphate (cAMP) in functions associated with the plasma membranes of Saccharomyces cerevisiae. Purified membranes from this source contained an adenylate cyclase which was insensitive to activation by fluoride or guanine nucleotides, only weakly responsive to chan...

journal_title：Molecular and cellular biology

authors： Jaynes PK,McDonough JP,Mahler HR

更新日期：1982-12-01 00:00:00

abstract：:In selective autophagy, the adaptor protein SQSTM1/p62 plays a critical role in recognizing/loading cargo (e.g., malfolded proteins) into autophagosomes for lysosomal degradation. Here we report that whereas SQSTM1/p62 levels fluctuated in a time-dependent manner during autophagy, inhibition or knockdown of Cdk9/cycli...

journal_title：Molecular and cellular biology

authors： Chen S,Zhou L,Zhang Y,Leng Y,Pei XY,Lin H,Jones R,Orlowski RZ,Dai Y,Grant S

更新日期：2014-09-15 00:00:00

abstract：:Using chromatin immunoprecipitation assays, we studied the 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)-mediated recruitment of the aryl hydrocarbon receptor (AhR) and several co-regulators to the CYP1A1 promoter. AhR displayed a time-dependent recruitment, reaching a peak at 75 min and maintaining promoter occupancy fo...

journal_title：Molecular and cellular biology

authors： Matthews J,Wihlén B,Thomsen J,Gustafsson JA

更新日期：2005-07-01 00:00:00

abstract：:During development and erythropoiesis, globin gene expression is finely modulated through an important network of transcription factors and chromatin modifying activities. In this report we provide in vivo evidence that endogenous Ikaros is recruited to the human beta-globin locus and targets the histone deacetylase H...

journal_title：Molecular and cellular biology

authors： Bottardi S,Ross J,Bourgoin V,Fotouhi-Ardakani N,Affar el B,Trudel M,Milot E

更新日期：2009-03-01 00:00:00

abstract：:The putative promoter of the variant surface glycoprotein (VSG) gene of Trypanosoma brucei was cloned into a plasmid containing the chloramphenicol acetyltransferase (CAT) gene. After electroporation into trypanosomes, this construct directed the expression of the CAT reporter gene. The essential region for promoter a...

journal_title：Molecular and cellular biology

authors： Jefferies D,Tebabi P,Pays E

更新日期：1991-01-01 00:00:00

abstract：:Nuclear extracts from chicken erythroid cells selectively stimulate transcription of the chicken histone H5 gene (and not of other chicken histone genes) after coinjection into frog oocytes. This effect is shown to involve an enhancerlike activity, and a region of the H5 gene sufficient to mediate trans-activation is ...

journal_title：Molecular and cellular biology

authors： Wigley PL,Wells JR

更新日期：1987-10-01 00:00:00

abstract：:Drugs that produce covalent interstrand cross-links (ICLs) in DNA remain central to the treatment of cancer, but the cell cycle checkpoints activated by ICLs have received little attention. We have used the fission yeast, Schizosaccharomyces pombe, to elucidate the checkpoint responses to the ICL-inducing anticancer d...

journal_title：Molecular and cellular biology

authors： Lambert S,Mason SJ,Barber LJ,Hartley JA,Pearce JA,Carr AM,McHugh PJ

更新日期：2003-07-01 00:00:00

abstract：:Skp2B, an F-box protein of unknown function, is frequently overexpressed in breast cancer. In order to determine the function of Skp2B and whether it has a role in breast cancer, we performed a two-hybrid screen and established transgenic mice expressing Skp2B in the mammary glands. We found that Skp2B interacts with ...

journal_title：Molecular and cellular biology

authors： Umanskaya K,Radke S,Chander H,Monardo R,Xu X,Pan ZQ,O'Connell MJ,Germain D

更新日期：2007-11-01 00:00:00

abstract：:A pool of Candida albicans RsaI fragments cloned onto a vector containing pBR322 sequences and the Candida ADE2 gene was used to transform a Candida ade2 mutant to adenine protrophy. A potential autonomously replicating sequence (ARS) in Candida DNA was identified by two criteria: instability of the selectable marker ...

journal_title：Molecular and cellular biology

authors： Kurtz MB,Cortelyou MW,Miller SM,Lai M,Kirsch DR

更新日期：1987-01-01 00:00:00

abstract：:In Saccharomyces cerevisiae, the GTP-binding Ypt1 protein (Ypt1p) is essential for endoplasmic reticulum-to-Golgi protein transport. By exploiting a GAL10-YPT1 fusion to regulate YPT1 expression, three multicopy suppressors, SLY2, SLY12, and SLY41, and a single-copy suppressor, SLY1-20, that allowed YPT1-independent g...

journal_title：Molecular and cellular biology

authors： Dascher C,Ossig R,Gallwitz D,Schmitt HD

更新日期：1991-02-01 00:00:00

abstract：:In resting cells, the NFAT1 transcription factor is kept inactive in the cytoplasm by phosphorylation on multiple serine residues. These phosphorylated residues are primarily contained within two types of serine-rich motifs, the SRR-1 and SP motifs, which are conserved within the NFAT family. Several different kinases...

journal_title：Molecular and cellular biology

authors： Okamura H,Garcia-Rodriguez C,Martinson H,Qin J,Virshup DM,Rao A

更新日期：2004-05-01 00:00:00

abstract：:We have cloned a human gene encoding the 70,000-dalton heat shock protein (HSP70) from a human genomic library, using the Drosophila HSP70 gene as a heterologous hybridization probe. The human recombinant clone hybridized to a 2.6-kilobase polyadenylated mRNA from HeLa cells exposed to 43 degrees C for 2 h. The 2.6-ki...

journal_title：Molecular and cellular biology

authors： Wu B,Hunt C,Morimoto R

更新日期：1985-02-01 00:00:00

abstract：:Methyl-accepting assays and a sensitive method for labeling specific CpG sites have been used to show that the DNA of F9 embryonal carcinoma cells decreases in 5-methylcytosine content by ca. 9% during retinoic acid-induced differentiation, whereas the DNA of dimethyl sulfoxide-induced Friend murine erythroleukemia (M...

journal_title：Molecular and cellular biology

authors： Bestor TH,Hellewell SB,Ingram VM

更新日期：1984-09-01 00:00:00

abstract：:Cells of Candida albicans WO-1 spontaneously switch between a white and opaque CFU, and this phase transition involves a dramatic change in cellular phenotype. By using a differential hybridization screen, an opaque-specific cDNA, Op1a, which represents the transcript of a gene regulated by switching, has been isolate...

journal_title：Molecular and cellular biology

authors： Morrow B,Srikantha T,Soll DR

更新日期：1992-07-01 00:00:00

abstract：:After flagellar detachment in Chlamydomonas reinhardi, there is a rapid synthesis and accumulation of mRNAs for tubulin and other flagellar proteins. Maximum levels of these mRNAs (flagellar RNAs) are reached within 1 h after deflagellation, after which they are rapidly degraded to their predeflagellation levels. The ...

journal_title：Molecular and cellular biology

authors： Baker EJ,Keller LR,Schloss JA,Rosenbaum JL

更新日期：1986-01-01 00:00:00

abstract：:The activities of the related Abl and Arg nonreceptor tyrosine kinases are kept under tight control in cells, but exposure to several different stimuli results in a two- to fivefold stimulation of kinase activity. Following the breakdown of inhibitory intramolecular interactions, Abl activation requires phosphorylatio...

journal_title：Molecular and cellular biology

authors： Tanis KQ,Veach D,Duewel HS,Bornmann WG,Koleske AJ

更新日期：2003-06-01 00:00:00

abstract：:The expression of three different actin genes in the sea urchin, Strongylocentrotus purpuratus, was monitored in embryos and adult tissues by using untranslated mRNA sequences as specific hybridization probes. Three distinct patterns of expression were found: muscle specific, embryo specific, and constitutive (i.e., p...

journal_title：Molecular and cellular biology

authors： Garcia R,Paz-Aliaga B,Ernst SG,Crain WR Jr

更新日期：1984-05-01 00:00:00

abstract：:SEC62 is required for the import of secretory protein precursors into the endoplasmic reticulum (ER) of Saccharomyces cerevisiae. The DNA sequence of SEC62 predicts a 32-kDa polypeptide with two potential membrane-spanning segments. Two antisera directed against different portions of the SEC62 coding region specifical...

journal_title：Molecular and cellular biology

authors： Deshaies RJ,Schekman R

更新日期：1990-11-01 00:00:00

abstract：:The human immunodeficiency virus type 1 (HIV-1) Tat protein activates transcription elongation by stimulating the Tat-activated kinase (TAK/p-TEFb), a protein kinase composed of CDK9 and its cyclin partner, cyclin T1. CDK9 is able to hyperphosphorylate the carboxyl-terminal domain (CTD) of the large subunit of RNA pol...

journal_title：Molecular and cellular biology

authors： Bourgeois CF,Kim YK,Churcher MJ,West MJ,Karn J

更新日期：2002-02-01 00:00:00

abstract：:Selective transcription of the insulin gene in pancreatic beta cells is regulated by its enhancer, located within the 5'-flanking region of the insulin gene. Transcription from the enhancer is controlled by both positive- and negative-acting cellular transcription factors. It was previously shown that both the 243- an...

journal_title：Molecular and cellular biology

authors： Stein RW,Whelan J

更新日期：1989-10-01 00:00:00

abstract：:With v-yes DNA as the probe, a human cDNA library made from placental RNA was screened under relaxed conditions, and DNA clones derived from a novel genetic locus, termed lyn, were obtained. Nucleotide sequencing revealed that lyn could encode a novel tyrosine kinase that was very similar to mouse T-lymphocyte-specifi...

journal_title：Molecular and cellular biology

authors： Yamanashi Y,Fukushige S,Semba K,Sukegawa J,Miyajima N,Matsubara K,Yamamoto T,Toyoshima K

更新日期：1987-01-01 00:00:00

abstract：:The Suppressor of the Hairy wing [Su(Hw)] binding region within the gypsy retrotransposon is the best known chromatin insulator in Drosophila melanogaster. According to previous data, two copies of the gypsy insulator inserted between an enhancer and a promoter neutralize each other's actions, which is indicative of a...

journal_title：Molecular and cellular biology

authors： Kravchenko E,Savitskaya E,Kravchuk O,Parshikov A,Georgiev P,Savitsky M

更新日期：2005-11-01 00:00:00

abstract：:The ADP/ATP translocator, a transmembrane protein of the mitochondrial inner membrane, is coded in Saccharomyces cerevisiae by the nuclear gene PET9. DNA sequence analysis of the PET9 gene showed that it encoded a protein of 309 amino acids which exhibited a high degree of homology with mitochondrial translocator prot...

journal_title：Molecular and cellular biology

authors： Adrian GS,McCammon MT,Montgomery DL,Douglas MG

更新日期：1986-02-01 00:00:00

abstract：:A special group of mitochondrial outer membrane proteins spans the membrane once, exposing soluble domains to both sides of the membrane. These proteins are synthesized in the cytosol and then inserted into the membrane by an unknown mechanism. To identify proteins that are involved in the biogenesis of the single-spa...

journal_title：Molecular and cellular biology

authors： Papić D,Elbaz-Alon Y,Koerdt SN,Leopold K,Worm D,Jung M,Schuldiner M,Rapaport D

更新日期：2013-10-01 00:00:00

abstract：:Barsoum and Varshavsky (Proc. Natl. Acad. Sci. U.S.A. 80:5330-5334, 1983) suggest that polypeptide mitogens and the mitogenic tumor promoter 12-O-tetradecanoylphorbol-13-acetate (TPA) stimulate gene amplification by related pathways. I demonstrated that TPA and the polypeptide mitogen fibroblast growth factor (FGF) bo...

journal_title：Molecular and cellular biology

authors： Herschman HR

更新日期：1985-05-01 00:00:00

abstract：:A murine monoclonal antibody was identified by its ability to induce a reversible antiproliferative effect on a human lymphoma cell line. Immunoprecipitation studies revealed that the antibody reacted with a 26-kilodalton cell surface protein (TAPA-1). A diverse group of human cell lines, including hematolymphoid, neu...

journal_title：Molecular and cellular biology

authors： Oren R,Takahashi S,Doss C,Levy R,Levy S

更新日期：1990-08-01 00:00:00

abstract：:The Ki-67 protein is widely used as a tumor proliferation marker. However, whether Ki-67 affects cell cycle progression has been controversial. Here we demonstrate that depletion of Ki-67 in human hTERT-RPE1, WI-38, IMR90, and hTERT-BJ cell lines and primary fibroblast cells slowed entry into S phase and coordinately ...

journal_title：Molecular and cellular biology

authors： Sun X,Bizhanova A,Matheson TD,Yu J,Zhu LJ,Kaufman PD

更新日期：2017-08-11 00:00:00

abstract：:Ret/ptc2 is a constitutively active, oncogenic form of the c-Ret receptor tyrosine kinase. Like the other papillary thyroid carcinoma forms of Ret, Ret/ptc2 is activated through fusion of the Ret tyrosine kinase domain to the dimerization domain of another protein. Investigation of requirements for Ret/ptc2 mitogenic ...

journal_title：Molecular and cellular biology

authors： Durick K,Gill GN,Taylor SS

更新日期：1998-04-01 00:00:00

abstract：:In a previous paper, we have shown that in the absence of stress, mouse embryonal carcinoma cells, like mouse early embryo multipotent cells, synthesize high levels of 89- and 70-kilodalton heat shock proteins (HSP)(O. Bensaude and M. Morange, EMBO J. 2:173-177, 1983). We report here the pattern of proteins synthesize...

journal_title：Molecular and cellular biology

authors： Morange M,Diu A,Bensaude O,Babinet C

更新日期：1984-04-01 00:00:00