A leaderless mRNA can bind to mammalian 80S ribosomes and direct polypeptide synthesis in the absence of translation initiation factors.

abstract：:Telomerase is a ribonucleoprotein enzyme that adds telomeric sequence repeats to the ends of linear chromosomes. In vitro, telomerase has been observed to add repeats to a DNA oligonucleotide primer in a processive manner, leading to the postulation of a DNA anchor site separate from the catalytic site of the enzyme. ...

journal_title：Molecular and cellular biology

authors： Hammond PW,Lively TN,Cech TR

更新日期：1997-01-01 00:00:00

abstract：:The class II transactivator (CIITA) is induced by gamma interferon (IFN-gamma) and activates major histocompatibility complex class II; however, this report shows it suppresses other genes. An N-terminal 36 amino acids of CIITA mediates suppression of the collagen alpha(2)(I) promoter via binding to CREB-binding prote...

journal_title：Molecular and cellular biology

authors： Zhu XS,Ting JP

更新日期：2001-10-01 00:00:00

abstract：:The retinoblastoma protein (RB) represses RNA polymerase III transcription effectively both in vivo and in vitro. Here we demonstrate that the general transcription factors snRNA-activating protein complex (SNAP(c)) and TATA binding protein (TBP) are important for RB repression of human U6 snRNA gene transcription by ...

journal_title：Molecular and cellular biology

authors： Hirsch HA,Gu L,Henry RW

更新日期：2000-12-01 00:00:00

abstract：:Translocation of preproteins across the mitochondrial outer membrane is mediated by the TOM complex. This complex consists of receptor components for the initial contact with preproteins at the mitochondrial surface and membrane-embedded proteins which promote transport and form the translocation pore. In order to und...

journal_title：Molecular and cellular biology

authors： Rapaport D,Künkele KP,Dembowski M,Ahting U,Nargang FE,Neupert W,Lill R

更新日期：1998-09-01 00:00:00

abstract：:An enzyme that hydrolyzes one PPi bond of the cap structure of mRNA, yielding m7GDP and 5'-p RNA was purified from Saccharomyces cerevisiae to a stage suitable for characterization. The specificity of the enzyme was studied, using both yeast mRNA and synthetic RNAs labeled in the cap structure. A synthetic capped RNA ...

journal_title：Molecular and cellular biology

authors： Stevens A

更新日期：1988-05-01 00:00:00

abstract：:We report in vivo analysis of histone and RNA polymerase II (pol II) occupancy at the 601 element, which functions as a strong in vitro nucleosome-positioning element and transcriptional pause site. Surprisingly, nucleosomes were not strongly positioned over the 601 element inserted either within a yeast chromosomal o...

journal_title：Molecular and cellular biology

authors： Perales R,Zhang L,Bentley D

更新日期：2011-08-01 00:00:00

abstract：:We find that immediately following transcript initiation, RNA polymerase II pauses at several locations even in the presence of relatively high (200 microM) levels of nucleoside triphosphates. Strong pauses with half-lives of >30 s were observed at +7, +18/19, and about +25 on the template used in these experiments. W...

journal_title：Molecular and cellular biology

authors： Pal M,Luse DS

更新日期：2002-01-01 00:00:00

abstract：:The divergently transcribed DIT1 and DIT2 genes of Saccharomyces cerevisiae, which belong to the mid-late class of sporulation-specific genes, are subject to Ssn6-Tup1-mediated repression in mitotic cells. The Ssn6-Tup1 complex, which is required for repression of diverse sets of coordinately regulated genes, is known...

journal_title：Molecular and cellular biology

authors： Rothfels K,Tanny JC,Molnar E,Friesen H,Commisso C,Segall J

更新日期：2005-08-01 00:00:00

abstract：:Approximately 30% of human tumors examined for mutations in polymerase beta (pol beta) appear to express pol beta variant proteins (D. Starcevic, S. Dalal, and J. B. Sweasy, Cell Cycle 3:998-1001, 2004). Many of these variants result from a single amino acid substitution. We have previously shown that the K289M and I2...

journal_title：Molecular and cellular biology

authors： Lang T,Dalal S,Chikova A,DiMaio D,Sweasy JB

更新日期：2007-08-01 00:00:00

abstract：:In thyroid hormone-depleted rats, the rate of transcription of the growth hormone (GH) gene in the anterior pituitary gland is lower than the rate in euthyroid controls, and there is a corresponding reduction in the abundance of the GH mRNA. Concomitantly, the poly(A) tail of the GH mRNA increases in length. Examinati...

journal_title：Molecular and cellular biology

authors： Murphy D,Pardy K,Seah V,Carter D

更新日期：1992-06-01 00:00:00

abstract：:The E1a gene of adenovirus encodes two proteins, 289 and 243 amino acids long, which have positive (transactivator) and negative (enhancer repressor) RNA polymerase II transcriptional regulatory properties and cell transformation activities including cooperation with an activated ras gene. The E1a transforming functio...

journal_title：Molecular and cellular biology

authors： Velcich A,Ziff E

更新日期：1988-05-01 00:00:00

abstract：:A cell-free nuclear replication system that is S-phase specific, that requires the activity of DNA polymerase alpha, and that is stimulated three- to eightfold by cytoplasmic factors from S-phase cells was used to examine the temporal specificity of chromosomal DNA synthesis in vitro. Temporal specificity of DNA synth...

journal_title：Molecular and cellular biology

authors： Heintz NH,Stillman BW

更新日期：1988-05-01 00:00:00

abstract：:Plasmid DNA substrates were X-irradiated and injected into the nuclei of Xenopus laevis oocytes. After incubation for 20 h, DNA was recovered from the oocytes and analyzed simultaneously for repair and for intermolecular homologous recombination by electrophoresis and bacterial transformation. Oocyte-mediated repair o...

journal_title：Molecular and cellular biology

authors： Sweigert SE,Carroll D

更新日期：1990-11-01 00:00:00

abstract：:Fibroblasts transformed by Fos oncogenes display increased expression of a number of genes implicated in tumor cell invasion and metastasis. In contrast to normal 208F rat fibroblasts, Fos-transformed 208F fibroblasts are growth factor independent for invasion. We demonstrate that invasion of v-Fos- or epidermal growt...

journal_title：Molecular and cellular biology

authors： Lamb RF,Hennigan RF,Turnbull K,Katsanakis KD,MacKenzie ED,Birnie GD,Ozanne BW

更新日期：1997-02-01 00:00:00

abstract：:Human antigen R (ELAVL1; HuR) is perhaps the best-characterized RNA-binding protein. Through its overexpression in various tumor types, HuR promotes posttranscriptional regulation of target genes in multiple core signaling pathways associated with tumor progression. The role of HuR overexpression in pancreatic tumorig...

journal_title：Molecular and cellular biology

authors： Peng W,Furuuchi N,Aslanukova L,Huang YH,Brown SZ,Jiang W,Addya S,Vishwakarma V,Peters E,Brody JR,Dixon DA,Sawicki JA

更新日期：2018-01-16 00:00:00

abstract：:The biosynthesis, phosphorylation, and degradation of the epidermal growth factor (EGF) receptor were examined in normal human fibroblasts. The receptor was initially synthesized as an Mr = 160,000 immature form which matured to an Mr = 170,000 form in a monensin-sensitive manner. Tunicamycin treatment led to the accu...

journal_title：Molecular and cellular biology

authors： Decker SJ

更新日期：1984-09-01 00:00:00

abstract：:Pax family transcription factors bind DNA through the paired domain. This domain, which is comprised of two helix-turn-helix motifs and a beta-hairpin structure, is a target of mutations in congenital disorders of mice and humans. Previously, we showed that Pax-5 (B-cell-specific activator protein) recruits proteins o...

journal_title：Molecular and cellular biology

authors： Wheat W,Fitzsimmons D,Lennox H,Krautkramer SR,Gentile LN,McIntosh LP,Hagman J

更新日期：1999-03-01 00:00:00

abstract：:The enhancer of yellow 1 gene, e(y)1, of Drosophila melanogaster has been cloned and demonstrated to encode the TAFII40 protein. The e(y)1 gene is expressed in females much more strongly than in males due to the accumulation of e(y)1 mRNA in the ovaries. Two different e(y)1 mutations have been obtained. The e(y)1(ul) ...

journal_title：Molecular and cellular biology

authors： Soldatov A,Nabirochkina E,Georgieva S,Belenkaja T,Georgiev P

更新日期：1999-05-01 00:00:00

abstract：:The GCN4 gene encodes a positive effector of amino acid biosynthetic genes in Saccharomyces cerevisiae. Genetic analysis has suggested that GCN4 is regulated by a hierarchy of interacting positive and negative effectors in response to amino acid starvation. Results presented here for a GCN4-lacZ gene fusion support th...

journal_title：Molecular and cellular biology

authors： Hinnebusch AG

更新日期：1985-09-01 00:00:00

abstract：:The hexanucleotide AAUAAA has been demonstrated to be part of the signal for cleavage and polyadenylation at appropriate sites on eucaryotic mRNA precursors. Since this sequence is not unique to polyadenylation sites, it cannot be the entire signal for the cleavage event. We have extended the definition of the polyade...

journal_title：Molecular and cellular biology

authors： Sadofsky M,Alwine JC

更新日期：1984-08-01 00:00:00

abstract：:Interleukin-5 (IL-5) is produced by T lymphocytes and known to support B-cell growth and eosinophilic differentiation of the progenitor cells. Using ATL-16T cells which express IL-5 mRNA, we have identified a region within the human IL-5 gene promoter that regulates IL-5 gene transcription. This cis-acting sequence co...

journal_title：Molecular and cellular biology

authors： Yamagata T,Nishida J,Sakai R,Tanaka T,Honda H,Hirano N,Mano H,Yazaki Y,Hirai H

更新日期：1995-07-01 00:00:00

abstract：:Human 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole sensitivity-inducing factor (DSIF) and negative elongation factor (NELF) negatively regulate transcription elongation by RNA polymerase II (RNAPII) in vitro. However, the physiological roles of this negative regulation are not well understood. Here, by using a num...

journal_title：Molecular and cellular biology

authors： Aida M,Chen Y,Nakajima K,Yamaguchi Y,Wada T,Handa H

更新日期：2006-08-01 00:00:00

abstract：:The levels of intramolecular plasmid recombination, following transfection of a plasmid substrate for homologous recombination into normal and immortally transformed cells, have been examined by two independent assays. In the first assay, recovered plasmid was tested for DNA rearrangements which regenerate a functiona...

journal_title：Molecular and cellular biology

authors： Finn GK,Kurz BW,Cheng RZ,Shmookler Reis RJ

更新日期：1989-09-01 00:00:00

abstract：:Previous results of our cDNA microarray analysis to look for genes whose expression level correlates well with in vitro tubulogenesis by NP31 endothelial cells revealed the transcription factor ATF3 known to be responsive to stress such as reactive oxygen species (ROS). Anti-ATF3 small interfering RNA gave an inhibito...

journal_title：Molecular and cellular biology

authors： Okamoto A,Iwamoto Y,Maru Y

更新日期：2006-02-01 00:00:00

abstract：:Homologous recombination of DNA molecules injected into Xenopus laevis oocyte nuclei is extremely efficient when those molecules are linear and have overlapping homologous ends. It was previously shown that a 5'----3' exonuclease activity in oocytes attacks injected linear DNAs and leaves them with single-stranded 3' ...

journal_title：Molecular and cellular biology

authors： Maryon E,Carroll D

更新日期：1991-06-01 00:00:00

abstract：:In mammals, the gene coding for apolipoprotein AI (apoAI), a protein of the plasma lipid transport system, is expressed only in the liver and the intestine. A series of plasmids containing various lengths of sequences flanking the 5' end of the human apoAI gene were constructed and assayed for transient expression aft...

journal_title：Molecular and cellular biology

authors： Sastry KN,Seedorf U,Karathanasis SK

更新日期：1988-02-01 00:00:00

abstract：:There exist in the Xenopus laevis genome clusters of tandemly repeated DNA sequences, consisting of two types of 393-base-pair repeating unit. Each such cluster contains several units of one of these paired tandem repeats (PTR-1), followed by several units of the other repeat (PTR-2). The number of repeats of each typ...

journal_title：Molecular and cellular biology

authors： Carroll D,Garrett JE,Lam BS

更新日期：1984-02-01 00:00:00

abstract：:Minichromosome maintenance protein 1 (Mcm1) is required for efficient replication of autonomously replicating sequence (ARS)-containing plasmids in yeast cells. Reduced DNA binding activity in the Mcm1-1 mutant protein (P97L) results in selective initiation of a subset of replication origins and causes instability of ...

journal_title：Molecular and cellular biology

authors： Chang VK,Donato JJ,Chan CS,Tye BK

更新日期：2004-07-01 00:00:00

abstract：:The androgen receptor (AR) is a ligand-activated transcription factor that interacts with coregulatory proteins during androgen-dependent gene regulation. Melanoma antigen gene protein 11 (MAGE-11) is an AR coregulator that specifically binds the AR NH(2)-terminal FXXLF motif and modulates the AR NH(2)- and carboxyl-t...

journal_title：Molecular and cellular biology

authors： Bai S,Wilson EM

更新日期：2008-03-01 00:00:00

abstract：:Mitogen-activated protein (MAP) kinase phosphatase 1 (MKP-1) has been shown to play a critical role in mediating the feedback control of MAP kinase cascades in a variety of cellular processes, including proliferation and stress responsiveness. Although MKP-1 expression is induced by a broad array of extracellular stim...

journal_title：Molecular and cellular biology

authors： Li J,Gorospe M,Hutter D,Barnes J,Keyse SM,Liu Y

更新日期：2001-12-01 00:00:00