Homologous plasmid recombination is elevated in immortally transformed cells.

abstract：:Mutations in the gene encoding methyl-CpG-binding protein 2 (MeCP2) lead to disrupted neuronal function and can cause the neurodevelopmental disorder Rett syndrome. MeCP2 is a transcriptional regulator that binds to methylated DNA and is most abundant in neuronal nuclei. The mechanisms by which MeCP2 regulates gene ex...

journal_title：Molecular and cellular biology

authors： Gonzales ML,Adams S,Dunaway KW,LaSalle JM

更新日期：2012-07-01 00:00:00

abstract：:As revealed by intracellular pools of nonactive Fas (Apo-1), export of Fas to the cell surface is often impaired in human tumors, thereby inactivating Fas ligand-mediated apoptosis. Here, we demonstrate that association with Fas-associated phosphatase 1 (FAP-1) attenuates Fas export to the cell surface. Forced express...

journal_title：Molecular and cellular biology

authors： Ivanov VN,Lopez Bergami P,Maulit G,Sato TA,Sassoon D,Ronai Z

更新日期：2003-05-01 00:00:00

abstract：:Telomerase is a ribonucleoprotein enzyme that adds telomeric sequence repeats to the ends of linear chromosomes. In vitro, telomerase has been observed to add repeats to a DNA oligonucleotide primer in a processive manner, leading to the postulation of a DNA anchor site separate from the catalytic site of the enzyme. ...

journal_title：Molecular and cellular biology

authors： Hammond PW,Lively TN,Cech TR

更新日期：1997-01-01 00:00:00

abstract：:DHCR24/seladin-1, a crucial enzyme in sterol synthesis, is of lower abundance in brain areas affected by Alzheimer's disease. While high levels of DHCR24/seladin-1 exert antiapoptotic function by conferring resistance against oxidative stress, the molecular mechanism for this protective effect is not fully understood....

journal_title：Molecular and cellular biology

authors： Kuehnle K,Crameri A,Kälin RE,Luciani P,Benvenuti S,Peri A,Ratti F,Rodolfo M,Kulic L,Heppner FL,Nitsch RM,Mohajeri MH

更新日期：2008-01-01 00:00:00

abstract：:Dominant and recessive mutations at the SSN20 locus were previously isolated as extragenic suppressors of mutations in three genes (SNF2, SNF5, and SNF6) that are required in trans to derepress invertase expression. All ssn20 alleles cause recessive, temperature-sensitive lethality. In this study we cloned the SSN20 g...

journal_title：Molecular and cellular biology

authors： Neigeborn L,Celenza JL,Carlson M

更新日期：1987-02-01 00:00:00

abstract：:To study the relationship between oncogenesis by v-src and normal cellular signalling pathways, we determined the effects of v-src on 3T3-TNR9 cells, a Swiss 3T3 variant which does not respond mitogenically to tumor promoters such as 12-O-tetradecanoyl-phorbol-13-acetate (TPA). We found that src was unable to transfor...

journal_title：Molecular and cellular biology

authors： Nori M,Shawver LK,Weber MJ

更新日期：1990-08-01 00:00:00

abstract：:Using nontumorigenic immortalized human cell lines KMST-6 (KMST) and SUSM-1 (SUSM), we attempted to identify the chromosome that carries a putative senescence-related gene(s). These cell lines are the only ones that have been established independently from normal human diploid fibroblasts following in vitro mutagenesi...

journal_title：Molecular and cellular biology

authors： Ogata T,Ayusawa D,Namba M,Takahashi E,Oshimura M,Oishi M

更新日期：1993-10-01 00:00:00

abstract：:The Integrator Complex is a group of proteins responsible for the endonucleolytic cleavage of primary small nuclear RNA (snRNA) transcripts within the nucleus. Integrator subunits 9 and 11 (IntS9/11) are thought to contain the catalytic activity based on their high sequence similarity to CPSF100 and CPSF73, which have...

journal_title：Molecular and cellular biology

authors： Albrecht TR,Wagner EJ

更新日期：2012-03-01 00:00:00

abstract：:The gene coding for the most abundant antifreeze protein (AFP) in the winter flounder was placed downstream of the Drosophila melanogaster hsp70 promoter and introduced into the D. melanogaster germ line by P-element-mediated transformation. In each of six transgenic strains tested, heat shock treatment induced the ex...

journal_title：Molecular and cellular biology

authors： Rancourt DE,Walker VK,Davies PL

更新日期：1987-06-01 00:00:00

abstract：:The putative promoter of the variant surface glycoprotein (VSG) gene of Trypanosoma brucei was cloned into a plasmid containing the chloramphenicol acetyltransferase (CAT) gene. After electroporation into trypanosomes, this construct directed the expression of the CAT reporter gene. The essential region for promoter a...

journal_title：Molecular and cellular biology

authors： Jefferies D,Tebabi P,Pays E

更新日期：1991-01-01 00:00:00

abstract：:In mammals, the gene coding for apolipoprotein AI (apoAI), a protein of the plasma lipid transport system, is expressed only in the liver and the intestine. A series of plasmids containing various lengths of sequences flanking the 5' end of the human apoAI gene were constructed and assayed for transient expression aft...

journal_title：Molecular and cellular biology

authors： Sastry KN,Seedorf U,Karathanasis SK

更新日期：1988-02-01 00:00:00

abstract：:Although DNA breakage and reunion in nonhomologous recombination are poorly understood, previous work suggests that short sequence homologies may play a role in the end-joining step in mammalian cells. To study the mechanism of end joining in more detail, we inserted a polylinker into the simian virus 40 T-antigen int...

journal_title：Molecular and cellular biology

authors： Roth DB,Wilson JH

更新日期：1986-12-01 00:00:00

abstract：:Injection of transcription activator-like effector nucleases (TALEN) mRNAs into mouse zygotes transferred into foster mothers efficiently generated founder mice with heritable mutations in targeted genes. Immunofluorescence visualization of phosphorylated histone 2A (γH2AX) combined with fluorescence in situ hybridiza...

journal_title：Molecular and cellular biology

authors： Li C,Qi R,Singleterry R,Hyle J,Balch A,Li X,Sublett J,Berns H,Valentine M,Valentine V,Sherr CJ

更新日期：2014-05-01 00:00:00

abstract：:An ssu72 mutant of Saccharomyces cerevisiae was identified as an enhancer of a TFIIB defect (sua7-1) that confers both a cold-sensitive growth defect and a downstream shift in transcription start site selection. The ssu72-1 allele did not affect cold sensitivity but, in combination with sua7-1, created a heat-sensitiv...

journal_title：Molecular and cellular biology

authors： Sun ZW,Hampsey M

更新日期：1996-04-01 00:00:00

abstract：:Lactoperoxidase was used to selectively radiolabel endocytic membrane. CHO cells were incubated with enzyme at 37 degrees C for 10 min to permit lactoperoxidase internalization. Radioiodination was done at 4 degrees C. About 90% of the radioiodinated products pelleted at 100,000 X g. From 12 to 15 different electropho...

journal_title：Molecular and cellular biology

authors： Storrie B,Dreesen TD,Maurey KM

更新日期：1981-03-01 00:00:00

abstract：:Nuclear hormone receptor coregulator (NRC) is a 2,063-amino-acid coregulator of nuclear hormone receptors and other transcription factors (e.g., c-Fos, c-Jun, and NF-kappaB). We and others have generated C57BL/6-129S6 hybrid (C57/129) NRC(+/-) mice that appear outwardly normal and grow and reproduce. In contrast, homo...

journal_title：Molecular and cellular biology

authors： Mahajan MA,Das S,Zhu H,Tomic-Canic M,Samuels HH

更新日期：2004-06-01 00:00:00

abstract：:In this report, we document an unusual mode of tissue-enriched gene expression that is primarily mediated by alternative and inefficient splicing. We have analyzed posttranscriptional regulation of the Drosophila erect wing gene, which provides a vital neuronal function and is essential for the formation of certain mu...

journal_title：Molecular and cellular biology

authors： Koushika SP,Soller M,DeSimone SM,Daub DM,White K

更新日期：1999-06-01 00:00:00

abstract：:Changes in endothelial nitric oxide synthase (eNOS) expression may be involved in the endothelium-dependent vasorelaxation dysfunction associated with several vascular diseases. In the present work, we demonstrate that eNOS mRNA contains a previously undescribed cis element in the 3' untranslated region (3' UTR). A U+...

journal_title：Molecular and cellular biology

authors： Alonso J,Sánchez de Miguel L,Montón M,Casado S,López-Farré A

更新日期：1997-10-01 00:00:00

abstract：:E3 ubiquitin ligases, which target specific molecules for proteolytic destruction, have emerged as key regulators of immune functions. Several E3 ubiquitin ligases, including c-Cbl, Cbl-b, GRAIL, Itch, and Nedd4, have been shown to negatively regulate T-cell activation. Here, we report that the HECT-type E3 ligase AIP...

journal_title：Molecular and cellular biology

authors： Chen A,Gao B,Zhang J,McEwen T,Ye SQ,Zhang D,Fang D

更新日期：2009-10-01 00:00:00

abstract：:Deacetylation of histones is carried out by a corepressor complex in which Sin3A is an essential scaffold protein. Two proteins in this complex, the Sin3A-associated proteins SAP30L and SAP30, have previously been suggested to function as linker molecules between various corepressors. In this report, we demonstrate ne...

journal_title：Molecular and cellular biology

authors： Viiri KM,Jänis J,Siggers T,Heinonen TY,Valjakka J,Bulyk ML,Mäki M,Lohi O

更新日期：2009-01-01 00:00:00

abstract：:Using competition mobility shift, methylation interference, and proteolytic clipping DNA binding assays, we demonstrate that the protein binding the major histocompatibility complex A beta CCAAT box is indistinguishable from the protein previously named NF-Y, which binds the major histocompatibility complex E alpha CC...

journal_title：Molecular and cellular biology

authors： Kern MJ,Woodward JG

更新日期：1991-01-01 00:00:00

abstract：:Repetitive DNA sequences have been implicated in the mediation of DNA rearrangement in mammalian cells. We have tested this hypothesis by using a dihydrofolate reductase (DHFR) expression vector into which candidate sequences were inserted. DHFR- Chinese hamster ovary (CHO) cells were transfected with this vector, the...

journal_title：Molecular and cellular biology

authors： Heartlein MW,Knoll JH,Latt SA

更新日期：1988-09-01 00:00:00

abstract：:The aryl hydrocarbon receptor (AhR) is a ligand-dependent transcription factor that can be activated by structurally diverse chemicals. To examine the mechanisms responsible for the promiscuity in AhR ligand binding, we determined the effects of mutations within the AhR ligand-binding domain (LBD) on the activity of d...

journal_title：Molecular and cellular biology

authors： Soshilov AA,Denison MS

更新日期：2014-05-01 00:00:00

abstract：:Regulatory regions of the mouse muscle creatine kinase (MCK) gene, previously discovered by analysis in cultured muscle cells, were analyzed in transgenic mice. The 206-bp MCK enhancer at nt-1256 was required for high-level expression of MCK-chloramphenicol acetyltransferase fusion genes in skeletal and cardiac muscle...

journal_title：Molecular and cellular biology

authors： Donoviel DB,Shield MA,Buskin JN,Haugen HS,Clegg CH,Hauschka SD

更新日期：1996-04-01 00:00:00

abstract：:Osteoclast differentiation factor (ODF)/receptor activator of NF-kappaB ligand is essential for inducing the differentiation of mature osteoclasts. We find that nuclear factor Y (NF-Y) binds to the CCAAT box on the ODF promoter and regulates its basal transcriptional activity. The CCAAT box on the ODF gene is required...

journal_title：Molecular and cellular biology

authors： Kabe Y,Yamada J,Uga H,Yamaguchi Y,Wada T,Handa H

更新日期：2005-01-01 00:00:00

abstract：:Sequence analysis of a mouse testicular alpha-tubulin partial cDNA, pRD alpha TT1, reveals an isotype that differs from both the somatic and the predominant testicular alpha tubulins at approximately 30% of the 212 amino acid residues determined. Although this mouse testicular cDNA retains the highly conserved sequenc...

journal_title：Molecular and cellular biology

authors： Hecht NB,Distel RJ,Yelick PC,Tanhauser SM,Driscoll CE,Goldberg E,Tung KS

更新日期：1988-02-01 00:00:00

abstract：:Expression of the recombinant human U1-70K protein in COS cells resulted in its rapid transport to the nucleus, even when binding to U1 RNA was debilitated. Deletion analysis of the U1-70K protein revealed the existence of two segments of the protein which were independently capable of nuclear localization. One nuclea...

journal_title：Molecular and cellular biology

authors： Romac JM,Graff DH,Keene JD

更新日期：1994-07-01 00:00:00

abstract：:We demonstrate that the immunoglobulin heavy-chain enhancer functions as the promoter for I mu sterile transcription. The enhancer itself, when placed 5' to the bacterial cat or neo genes, is able to direct transcription by using heterogeneous start sites that are generally the same as those found with bona fide I mu ...

journal_title：Molecular and cellular biology

authors： Su LK,Kadesch T

更新日期：1990-06-01 00:00:00

abstract：:Tristetraprolin (TTP) regulates the expression of AU-rich element-containing mRNAs through promoting the degradation and repressing the translation of target mRNA. While the mechanism for promoting target mRNA degradation has been extensively studied, the mechanism underlying translational repression is not well estab...

journal_title：Molecular and cellular biology

authors： Tao X,Gao G

更新日期：2015-11-01 00:00:00

abstract：:Plasmids containing the hormone regulatory element of mouse mammary tumor virus linked to the thymidine kinase promoter of herpes simplex virus and the reporter gene chloramphenicol acetyltransferase of Escherichia coli respond to glucocorticoids and progestins when transfected into appropriate cells. In the human mam...

journal_title：Molecular and cellular biology

authors： Piña B,Haché RJ,Arnemann J,Chalepakis G,Slater EP,Beato M

更新日期：1990-02-01 00:00:00