Isolated clusters of paired tandemly repeated sequences in the Xenopus laevis genome.

abstract：:An elastase-human growth hormone (hGH) fusion gene containing 205 base pairs of elastase 5' flanking region is expressed exclusively in pancreatic acinar cells of transgenic mice. This paper shows that the promoter region (-72 to +8) and the enhancer (-205 to -73) function independently of each other. The elastase enh...

journal_title：Molecular and cellular biology

authors： Ornitz DM,Hammer RE,Davison BL,Brinster RL,Palmiter RD

更新日期：1987-10-01 00:00:00

abstract：:Peroxisomal PTS2-dependent matrix protein import starts with the recognition of the PTS2 targeting signal by the import receptor Pex7p. Subsequently, the formed Pex7p/cargo complex is transported from the cytosol to the peroxisomal docking complex, consisting of Pex13p and Pex14p. In Saccharomyces cerevisiae, the latt...

journal_title：Molecular and cellular biology

authors： Stein K,Schell-Steven A,Erdmann R,Rottensteiner H

更新日期：2002-09-01 00:00:00

abstract：:The Saccharomyces cerevisiae pheromone a-factor is produced by a cells and interacts with alpha cells to cause cell cycle arrest and other physiological responses associated with mating. Two a-factor structural genes, MFA1 and MFA2, have been previously cloned with synthetic probes based on the a-factor amino acid seq...

journal_title：Molecular and cellular biology

authors： Michaelis S,Herskowitz I

更新日期：1988-03-01 00:00:00

abstract：:Serum induction of c-jun expression in HeLa cells requires a MEF2 site at -59 in the c-jun promoter. MEF2 sites, found in many muscle-specific enhancers, are bound by a family of transcription factors, MEF2A through -D, which are related to serum response factor in their DNA binding domains. We have found that MEF2D i...

journal_title：Molecular and cellular biology

authors： Han TH,Prywes R

更新日期：1995-06-01 00:00:00

abstract：:The polycomb protein Bmi-1 represses the INK4a locus, which encodes the tumor suppressors p16 and p14(ARF). Here we report that Bmi-1 is downregulated when WI-38 human fibroblasts undergo replicative senescence, but not quiescence, and extends replicative life span when overexpressed. Life span extension by Bmi-1 requ...

journal_title：Molecular and cellular biology

authors： Itahana K,Zou Y,Itahana Y,Martinez JL,Beausejour C,Jacobs JJ,Van Lohuizen M,Band V,Campisi J,Dimri GP

更新日期：2003-01-01 00:00:00

abstract：:The NuA4 histone acetyltransferase (HAT) multisubunit complex is responsible for acetylation of histone H4 and H2A N-terminal tails in yeast. Its catalytic component, Esa1, is essential for cell cycle progression, gene-specific regulation and has been implicated in DNA repair. Almost all NuA4 subunits have clear homol...

journal_title：Molecular and cellular biology

authors： Doyon Y,Selleck W,Lane WS,Tan S,Côté J

更新日期：2004-03-01 00:00:00

abstract：:In the yeast Saccharomyces cerevisiae, yeast RAS proteins are potent activators of adenylate cyclase. In the present work we measured the activity of adenylate cyclase in membranes from Saccharomyces cerevisiae which overexpress this enzyme. The response of the enzyme to added RAS2 proteins bound with various guanine ...

journal_title：Molecular and cellular biology

authors： Field J,Broek D,Kataoka T,Wigler M

更新日期：1987-06-01 00:00:00

abstract：:The cytotoxic serine protease B (CSP-B) gene is activated during cytotoxic T-lymphocyte maturation. In this report, we demonstrate that the PEER T-cell line (bearing gamma/delta T-cell receptors) accumulates CSP-B mRNA following exposure to 12-O-tetradecanoylphorbol-13-acetate (TPA) and N6-2'-O-dibutyryladenosine 3',5...

journal_title：Molecular and cellular biology

authors： Hanson RD,Ley TJ

更新日期：1990-11-01 00:00:00

abstract：:Inactivation of DNA mismatch repair by mutation or by transcriptional silencing of the MLH1 gene results in genome instability and cancer predisposition. We recently found (P. V. Shcherbakova and T. A. Kunkel, Mol. Cell. Biol. 19:3177-3183, 1999) that an elevated spontaneous mutation rate can also result from increase...

journal_title：Molecular and cellular biology

authors： Shcherbakova PV,Hall MC,Lewis MS,Bennett SE,Martin KJ,Bushel PR,Afshari CA,Kunkel TA

更新日期：2001-02-01 00:00:00

abstract：:The Drosophila melanogaster GAGA factor (encoded by the Trithorax-like [Trl] gene) is required for correct chromatin architecture at diverse chromosomal sites. The Trl gene encodes two alternatively spliced isoforms of the GAGA factor (GAGA-519 and GAGA-581) that are identical except for the length and sequence of the...

journal_title：Molecular and cellular biology

authors： Greenberg AJ,Schedl P

更新日期：2001-12-01 00:00:00

abstract：:USF is a family of basic helix-loop transcriptional factors that recognizes DNA-binding sites similar to those of the Myc oncoproteins. Here, various functional domains in the mouse USF2 protein were identified and characterized. Indirect immunofluorescence studies with transiently transfected cells revealed that both...

journal_title：Molecular and cellular biology

authors： Luo X,Sawadogo M

更新日期：1996-04-01 00:00:00

abstract：:The signal-transducing adaptor protein 2 (STAP-2) is a recently identified adaptor protein that contains a pleckstrin homology (PH) and Src homology 2 (SH2)-like domains, as well as a proline-rich domain in its C-terminal region. In previous studies, we demonstrated that STAP-2 binds to MyD88 and IKK-alpha or IKK-beta...

journal_title：Molecular and cellular biology

authors： Ikeda O,Sekine Y,Yasui T,Oritani K,Sugiyma K,Muromoto R,Ohbayashi N,Yoshimura A,Matsuda T

更新日期：2008-08-01 00:00:00

abstract：:We have recently reported that the yeast chromatin-remodeling factor Swi1 can exist as a prion, [SWI(+)], demonstrating a link between prionogenesis and global transcriptional regulation. To shed light on how the Swi1 conformational switch influences Swi1 function and to define the sequence and structural requirements...

journal_title：Molecular and cellular biology

authors： Du Z,Crow ET,Kang HS,Li L

更新日期：2010-10-01 00:00:00

abstract：:Mammalian eukaryotic translation initiation factor 4F (eIF4F) is a cap-binding protein complex consisting of three subunits: eIF4E, eIF4A, and eIF4G. In yeast and plants, two related eIF4G species are encoded by two different genes. To date, however, only one functional eIF4G polypeptide, referred to here as eIF4GI, h...

journal_title：Molecular and cellular biology

authors： Gradi A,Imataka H,Svitkin YV,Rom E,Raught B,Morino S,Sonenberg N

更新日期：1998-01-01 00:00:00

abstract：:Telomerase is a ribonucleoprotein reverse transcriptase responsible for the maintenance of one strand of the telomere terminal repeats. The catalytic protein subunit of the telomerase complex, known as TERT, possesses a reverse transcriptase (RT) domain that mediates nucleotide addition. The RT domain of TERT is disti...

journal_title：Molecular and cellular biology

authors： Lue NF,Lin YC,Mian IS

更新日期：2003-12-01 00:00:00

abstract：:Rpd3(L) and Rpd3(S) are distinct multisubunit complexes containing the Rpd3 histone deacetylase. Disruption of the GCN5 histone acetyltransferase gene shows a strong synthetic phenotype when combined with either an sds3 mutation affecting only the Rpd3(L) complex or an rco1 mutation affecting only Rpd3(S). However, th...

journal_title：Molecular and cellular biology

authors： Biswas D,Takahata S,Stillman DJ

更新日期：2008-07-01 00:00:00

abstract：:IRE1-alpha is an integral membrane protein of the endoplasmic reticulum (ER) that is a key sensor in the cellular transcriptional response to stress in the ER. Upon induction of ER stress, IRE1-alpha is activated, resulting in the synthesis of the active form of the transcription factor XBP1 via IRE1-mediated splicing...

journal_title：Molecular and cellular biology

authors： Huang ZM,Tan T,Yoshida H,Mori K,Ma Y,Yen TS

更新日期：2005-09-01 00:00:00

abstract：:The transcription and DNA repair factor TFIIH is composed of 10 subunits. Mutations in the XPB, XPD, and p8 subunits are genetically linked to human diseases, including cancer. However, no reports of mutations in other TFIIH subunits have been reported in higher eukaryotes. Here, we analyze at genetic, molecular, and ...

journal_title：Molecular and cellular biology

authors： Fregoso M,Lainé JP,Aguilar-Fuentes J,Mocquet V,Reynaud E,Coin F,Egly JM,Zurita M

更新日期：2007-05-01 00:00:00

abstract：:Thyroid hormone (T(3)) activates nuclear receptor transcription factors, encoded by the TRalpha (NR1A1) and TRbeta (NR1A2) genes, to regulate target gene expression. Several TR isoforms exist, and studies of null mice have identified some unique functions for individual TR variants, although considerable redundancy oc...

journal_title：Molecular and cellular biology

authors： Williams GR

更新日期：2000-11-01 00:00:00

abstract：:Activation of transforming potential of the cellular raf gene has uniformly been associated with the deletion of amino-terminal coding sequences. In order to determine whether 5' truncation alone could activate cellular raf, we constructed 21 human c-raf-1 cDNAs with variable BAL 31-generated deletions distal to a Mol...

journal_title：Molecular and cellular biology

authors： Stanton VP Jr,Nichols DW,Laudano AP,Cooper GM

更新日期：1989-02-01 00:00:00

abstract：:Sequencing studies have shown that in somatic cells alternating runs of purines and pyrimidines are frequently associated with recombination crossover points. To test whether such sequences actually promote recombination, we have examined the effects of poly[d(pGpT).d(pApC)] and poly[d(pCpG).d(pCpG)] repeats on a homo...

journal_title：Molecular and cellular biology

authors： Bullock P,Miller J,Botchan M

更新日期：1986-11-01 00:00:00

abstract：:Mrc1 is a conserved checkpoint mediator protein that transduces the replication stress signal to the downstream effector kinase. The loss of mrc1 checkpoint activity results in the aberrant activation of late/dormant origins in the presence of hydroxyurea. Mrc1 was also suggested to regulate orders of early origin fir...

journal_title：Molecular and cellular biology

authors： Matsumoto S,Kanoh Y,Shimmoto M,Hayano M,Ueda K,Fukatsu R,Kakusho N,Masai H

更新日期：2017-03-17 00:00:00

abstract：:By using a retrovirus-derived vector system, we generated derivatives of the human colon cancer cell line HT29 that stably overexpress a full-length cDNA encoding the beta 1 isoform of rat protein kinase C (PKC). Two of these cell lines, PKC6 and PKC7, displayed an 11- to 15-fold increase in PKC activity when compared...

journal_title：Molecular and cellular biology

authors： Choi PM,Tchou-Wong KM,Weinstein IB

更新日期：1990-09-01 00:00:00

abstract：:The Asf1 and Rad6 pathways have been implicated in a number of common processes such as suppression of gross chromosomal rearrangements (GCRs), DNA repair, modification of chromatin, and proper checkpoint functions. We examined the relationship between Asf1 and different gene products implicated in postreplication rep...

journal_title：Molecular and cellular biology

authors： Kats ES,Enserink JM,Martinez S,Kolodner RD

更新日期：2009-10-01 00:00:00

abstract：:Different models have been proposed explaining how eukaryotic gene transcription is terminated. Recently, Nsi1, a factor involved in silencing of ribosomal DNA (rDNA), was shown to be required for efficient termination of rDNA transcription by RNA polymerase I (Pol I) in the yeast Saccharomyces cerevisiae. Nsi1 contai...

journal_title：Molecular and cellular biology

authors： Merkl P,Perez-Fernandez J,Pilsl M,Reiter A,Williams L,Gerber J,Böhm M,Deutzmann R,Griesenbeck J,Milkereit P,Tschochner H

更新日期：2014-10-01 00:00:00

abstract：:SRC family kinases play essential roles in a variety of cellular functions, including proliferation, survival, differentiation, and apoptosis. The activities of these kinases are regulated by intramolecular interactions and by heterologous binding partners that modulate the transition between active and inactive struc...

journal_title：Molecular and cellular biology

authors： Burnham MR,Bruce-Staskal PJ,Harte MT,Weidow CL,Ma A,Weed SA,Bouton AH

更新日期：2000-08-01 00:00:00

abstract：:The cytokine gamma interferon (IFN-gamma) and the calcitropic steroid hormone 1,25-dihydroxyvitamin D (1,25D) are activators of macrophage immune function. In sarcoidosis, tuberculosis, and several granulomatoses, IFN-gamma induces 1,25D synthesis by macrophages and inhibits 1,25D induction of 24-hydroxylase, a key en...

journal_title：Molecular and cellular biology

authors： Vidal M,Ramana CV,Dusso AS

更新日期：2002-04-01 00:00:00

abstract：:Extinction of phosphoenolpyruvate carboxykinase (PCK) gene expression in hepatoma x fibroblast hybrids is mediated by a trans-acting genetic locus designated tissue-specific extinguisher 1 (TSE1). To identify PCK gene sequences required for extinction, hepatoma transfectants expressing PCK-thymidine kinase (TK) chimer...

journal_title：Molecular and cellular biology

authors： Thayer MJ,Lugo TG,Leach RJ,Fournier RE

更新日期：1990-06-01 00:00:00

abstract：:Illegitimate recombination events in mammalian cells often contain extraneous nucleotides or filler DNA at the recombinant joins. The polyomavirus-transformed cell line 7axB has previously been found to contain 37 base pairs (bp) of filler DNA at one virus-host join of the single insert of integrated viral DNA (A. Hay...

journal_title：Molecular and cellular biology

authors： Williams TJ,Fried M

更新日期：1986-06-01 00:00:00

abstract：:We analyzed the transcriptional properties of a repetitive sequence element, BmX, that belongs to a large gene family (approximately 2 x 10(4) copies) in the genome of the Bombyx mori silkworm. We discovered BmX elements because of their ability to direct transcription by polymerase III in vitro and used them to test ...

journal_title：Molecular and cellular biology

authors： Wilson ET,Condliffe DP,Sprague KU

更新日期：1988-02-01 00:00:00